LOCUS       NM_033362                959 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein S12 (MRPS12),
            transcript variant 2, mRNA.
ACCESSION   NM_033362
VERSION     NM_033362.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 959)
  AUTHORS   Qiu,X., Guo,D., Du,J., Bai,Y. and Wang,F.
  TITLE     A novel biomarker, MRPS12 functions as a potential oncogene in
            ovarian cancer and is a promising prognostic candidate
  JOURNAL   Medicine (Baltimore) 100 (8), e24898 (2021)
   PUBMED   33663122
  REMARK    GeneRIF: A novel biomarker, MRPS12 functions as a potential
            oncogene in ovarian cancer and is a promising prognostic candidate.
REFERENCE   3  (bases 1 to 959)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   4  (bases 1 to 959)
  AUTHORS   Amunts,A., Brown,A., Toots,J., Scheres,S.H.W. and Ramakrishnan,V.
  TITLE     Ribosome. The structure of the human mitochondrial ribosome
  JOURNAL   Science 348 (6230), 95-98 (2015)
   PUBMED   25838379
REFERENCE   5  (bases 1 to 959)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (bases 1 to 959)
  AUTHORS   Cavdar Koc,E., Burkhart,W., Blackburn,K., Moseley,A. and
            Spremulli,L.L.
  TITLE     The small subunit of the mammalian mitochondrial ribosome.
            Identification of the full complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (22), 19363-19374 (2001)
   PUBMED   11279123
REFERENCE   7  (bases 1 to 959)
  AUTHORS   Mariottini,P., Shah,Z.H., Toivonen,J.M., Bagni,C., Spelbrink,J.N.,
            Amaldi,F. and Jacobs,H.T.
  TITLE     Expression of the gene for mitoribosomal protein S12 is controlled
            in human cells at the levels of transcription, RNA splicing, and
            translation
  JOURNAL   J Biol Chem 274 (45), 31853-31862 (1999)
   PUBMED   10542210
REFERENCE   8  (bases 1 to 959)
  AUTHORS   Johnson,D.F., Hamon,M. and Fischel-Ghodsian,N.
  TITLE     Characterization of the human mitochondrial ribosomal S12 gene
  JOURNAL   Genomics 52 (3), 363-368 (1998)
   PUBMED   9790755
REFERENCE   9  (bases 1 to 959)
  AUTHORS   Shah,Z.H., Migliosi,V., Miller,S.C., Wang,A., Friedman,T.B. and
            Jacobs,H.T.
  TITLE     Chromosomal locations of three human nuclear genes (RPSM12, TUFM,
            and AFG3L1) specifying putative components of the mitochondrial
            gene expression apparatus
  JOURNAL   Genomics 48 (3), 384-388 (1998)
   PUBMED   9545647
REFERENCE   10 (bases 1 to 959)
  AUTHORS   Shah,Z.H., O'Dell,K.M., Miller,S.C., An,X. and Jacobs,H.T.
  TITLE     Metazoan nuclear genes for mitoribosomal protein S12
  JOURNAL   Gene 204 (1-2), 55-62 (1997)
   PUBMED   9434165
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011455.6 and BC007499.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_033362.3.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 28S subunit protein that belongs to
            the ribosomal protein S12P family. The encoded protein is a key
            component of the ribosomal small subunit and controls the decoding
            fidelity and susceptibility to aminoglycoside antibiotics. The gene
            for mitochondrial seryl-tRNA synthetase is located upstream and
            adjacent to this gene, and both genes are possible candidates for
            the autosomal dominant deafness gene (DFNA4). Splice variants that
            differ in the 5' UTR have been found for this gene; all three
            variants encode the same protein. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1, 2, and 3 encode the same
            isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006424.1, CB126732.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000308018.9/
                                                  ENSP00000308845.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AC011455.6         84103-84113
            12-811              BC007499.2         1-800
            812-959             AC011455.6         86180-86327
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..959
                     /gene="MRPS12"
                     /gene_synonym="MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12;
                     uS12m"
                     /note="mitochondrial ribosomal protein S12"
                     /db_xref="GeneID:6183"
                     /db_xref="HGNC:HGNC:10380"
                     /db_xref="MIM:603021"
     exon            1..55
                     /gene="MRPS12"
                     /gene_synonym="MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12;
                     uS12m"
                     /inference="alignment:Splign:2.1.0"
     exon            56..123
                     /gene="MRPS12"
                     /gene_synonym="MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12;
                     uS12m"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..491
                     /gene="MRPS12"
                     /gene_synonym="MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12;
                     uS12m"
                     /note="28S ribosomal protein S12, mitochondrial; S12mt;
                     MRP-S12; mitochondrial small ribosomal subunit protein
                     uS12m"
                     /codon_start=1
                     /product="small ribosomal subunit protein uS12m precursor"
                     /protein_id="NP_203526.1"
                     /db_xref="CCDS:CCDS12525.1"
                     /db_xref="GeneID:6183"
                     /db_xref="HGNC:HGNC:10380"
                     /db_xref="MIM:603021"
                     /translation="MSWSGLLHGLNTSLTCGPALVPRLWATCSMATLNQMHRLGPPKR
                     PPRKLGPTEGRPQLKGVVLCTFTRKPKKPNSANRKCCRVRLSTGREAVCFIPGEGHTL
                     QEHQIVLVEGGRTQDLPGVKLTVVRGKYDCGHVQKK"
     exon            124..959
                     /gene="MRPS12"
                     /gene_synonym="MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12;
                     uS12m"
                     /inference="alignment:Splign:2.1.0"
     regulatory      781..786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS12"
                     /gene_synonym="MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12;
                     uS12m"
                     /note="hexamer: AATAAA"
     polyA_site      811
                     /gene="MRPS12"
                     /gene_synonym="MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12;
                     uS12m"
                     /note="major polyA site"
     regulatory      927..932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS12"
                     /gene_synonym="MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12;
                     uS12m"
                     /note="hexamer: ATTAAA"
     polyA_site      959
                     /gene="MRPS12"
                     /gene_synonym="MPR-S12; MT-RPS12; RPMS12; RPS12; RPSM12;
                     uS12m"
ORIGIN      
        1 agaggctaga agctggattc agcgtgtccg cgacctcacc tttaggtcct gtgagggacg
       61 gcccaggtgg caggatgtcc tggtctggcc ttctccatgg cctcaacacg tccctaactt
      121 gtggcccagc tctggttccc cggctctggg ctacctgctc catggctacc ctgaaccaga
      181 tgcaccgcct ggggcccccc aagcggccgc ctcggaagct gggccccacg gaaggccggc
      241 cgcagctgaa gggtgtggtc ctgtgcacgt ttacccgcaa gccgaagaag cccaactcag
      301 ccaatcgcaa gtgctgtcga gtgcggctca gcactggccg cgaggccgtc tgcttcatcc
      361 ctggggaggg ccacaccctg caggagcacc agattgtcct tgtggagggc ggccgcaccc
      421 aggacctgcc aggcgtcaag ctcaccgttg tgcgtggcaa gtacgactgt ggccacgtgc
      481 agaagaagtg acggctgggg gcacagtggg ctgggcgccc ctgcagaaca tgaaccttcc
      541 gctcctggct gccacagggt cctccgatgc tggcctttgc gcctctagag gcagccactc
      601 atggattcaa gtcctggctc cgcctcttcc atcaggacca ctattaagcc ataggagtcc
      661 tgggggtgca aagggtgccc ctctgtcaac acccttggct cctgtgttta gaggggtggc
      721 ctgaaggacc ttttctgctg ggacaagaca ctgtactgcc ctctgctggg aaggggtttt
      781 aataaacaga ccctggcgct tgtgatgtaa atccccttgt ggagtatttg ccctctgtgt
      841 ggctgtggca gtggacatct ctgagcttca gtttcctcca gaaaatagct ccattggtgg
      901 tatctccttt gtttggtttg gtgaagatta aattcgatgc tctatgtgca taacacgca
//
