LOCUS       NM_032351               1553 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein L45 (MRPL45),
            transcript variant 1, mRNA.
ACCESSION   NM_032351 XM_936540
VERSION     NM_032351.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Itoh,Y., Andrell,J., Choi,A., Richter,U., Maiti,P., Best,R.B.,
            Barrientos,A., Battersby,B.J. and Amunts,A.
  TITLE     Mechanism of membrane-tethered mitochondrial protein synthesis
  JOURNAL   Science 371 (6531), 846-849 (2021)
   PUBMED   33602856
REFERENCE   3  (bases 1 to 1553)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1553)
  AUTHORS   Brown,A., Rathore,S., Kimanius,D., Aibara,S., Bai,X.C., Rorbach,J.,
            Amunts,A. and Ramakrishnan,V.
  TITLE     Structures of the human mitochondrial ribosome in native states of
            assembly
  JOURNAL   Nat Struct Mol Biol 24 (10), 866-869 (2017)
   PUBMED   28892042
REFERENCE   5  (bases 1 to 1553)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   6  (bases 1 to 1553)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   7  (bases 1 to 1553)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   8  (bases 1 to 1553)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1553)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   10 (bases 1 to 1553)
  AUTHORS   Koc,E.C., Burkhart,W., Blackburn,K., Moyer,M.B., Schlatzer,D.M.,
            Moseley,A. and Spremulli,L.L.
  TITLE     The large subunit of the mammalian mitochondrial ribosome. Analysis
            of the complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (47), 43958-43969 (2001)
   PUBMED   11551941
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK293605.1 and BC006235.2.
            
            On Nov 24, 2018 this sequence version replaced NM_032351.5.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 39S subunit protein. Alternative
            splicing results in multiple transcript variants. Pseudogenes
            corresponding to this gene are found on chromosomes 2p and 17q.
            [provided by RefSeq, May 2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK172741.1, SRR5189661.101169.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 11551941; reported by
                                                  MitoCarta
            MANE Ensembl match                 :: ENST00000613675.5/
                                                  ENSP00000484903.1
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1126              AK293605.1         112-1237
            1127-1553           BC006235.2         1111-1537
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q12"
     gene            1..1553
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /note="mitochondrial ribosomal protein L45"
                     /db_xref="GeneID:84311"
                     /db_xref="HGNC:HGNC:16651"
                     /db_xref="MIM:611850"
     exon            1..82
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..937
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /note="isoform 1 is encoded by transcript variant 1; 39S
                     ribosomal protein L45, mitochondrial; mitochondrial large
                     ribosomal subunit protein mL45"
                     /codon_start=1
                     /product="large ribosomal subunit protein mL45 isoform 1"
                     /protein_id="NP_115727.5"
                     /db_xref="CCDS:CCDS11326.1"
                     /db_xref="GeneID:84311"
                     /db_xref="HGNC:HGNC:16651"
                     /db_xref="MIM:611850"
                     /translation="MAAPIPQGFSCLSRFLGWWSRQPVLVTQSAAIVPVRTKKRFTPP
                     IYQPKFKTEKEFMQHARKAGLVIPPEKSDRSIHLACTAGIFDAYVPPEGDARISSLSK
                     EGLIERTERMKKTMASQVSIRRIKDYDANFKIKDFPEKAKDIFIEAHLCLNNSDHDRL
                     HTLVTEHCFPDMTWDIKYKTVRWSFVESLEPSHVVQVRCSSMMNQGNVYGQITVRMHT
                     RQTLAIYDRFGRLMYGQEDVPKDVLEYVVFEKQLTNPYGSWRMHTKIVPPWAPPKQPI
                     LKTVMIPGPQLKPEEEYEEAQGEAQKPQLA"
     exon            83..260
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /inference="alignment:Splign:2.1.0"
     exon            261..378
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /inference="alignment:Splign:2.1.0"
     exon            379..477
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /inference="alignment:Splign:2.1.0"
     exon            478..526
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /inference="alignment:Splign:2.1.0"
     exon            527..676
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /inference="alignment:Splign:2.1.0"
     exon            677..850
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /inference="alignment:Splign:2.1.0"
     exon            851..1553
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1531..1536
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /note="hexamer: TATAAA"
     polyA_site      1553
                     /gene="MRPL45"
                     /gene_synonym="L45mt; Mba1; mL45; MRP-L45"
                     /note="major polyA site"
ORIGIN      
        1 cctttgcggg aacaagatgg cagcccccat acctcaaggg ttctcttgtt tatcgaggtt
       61 tttgggctgg tggtctcggc agccagttct ggtgactcag tccgcagcta tagttccagt
      121 aagaactaaa aaacgtttca cacctcctat ttatcaacct aaatttaaaa cagaaaagga
      181 gtttatgcaa catgcccgga aagcaggatt ggttattcct ccagaaaaat cggaccgttc
      241 catacatctg gcctgtacag ctggtatatt tgatgcctat gttcctcctg agggtgatgc
      301 acgcatatca tctctttcaa aggagggact gatagagaga actgaacgaa tgaagaagac
      361 tatggcatca caagtgtcaa tccggaggat aaaagactat gatgccaact ttaaaataaa
      421 ggacttccct gaaaaagcta aggatatctt tattgaagct cacctttgtc taaataactc
      481 agaccatgac cggcttcata ccttggtaac tgaacactgt tttccagaca tgacttggga
      541 catcaaatat aagaccgtcc gctggagctt tgtggaatct ttagagccct ctcatgttgt
      601 tcaagttcgc tgttcaagta tgatgaacca gggcaacgtg tacggccaga tcaccgtacg
      661 catgcacacc cggcagactc tggccatcta tgaccggttt ggccggttga tgtatggaca
      721 ggaagatgta cccaaggatg tcctggagta tgttgtattc gaaaagcagt tgacaaaccc
      781 ctatggaagc tggagaatgc ataccaagat cgttccccca tgggcacccc ctaagcagcc
      841 catccttaag acggtgatga tccctggccc tcagctgaaa ccagaagaag aatatgaaga
      901 ggcacaagga gaggcccaga agcctcagct agcctgatga caaaaatgac ttctagggtg
      961 aagcctgggt gatgaggctg ctggaagctt tgaagtctcc cattcccctc atgctataaa
     1021 aagaactacc tttgttctct cccatcctgc tcaggtcttt tcagcagtct catcatcagc
     1081 aaccatgact gatgactggg ccctagcagg tggcaggtat aacatggcca tggacactct
     1141 tcttttttaa attttatgtc tagcttctga gtctagatga aagacagtat gtttcagaga
     1201 acattggata tcagtttttc ccacagcagg gactgtgaga gacaaccagc agcatcctct
     1261 ttgtaatcac agggcaggga tcagagtttg aaatgaaatg ttgtcagggt gttggaaaaa
     1321 ttttggtgag ttctgcacat ttcccctggt tcaggctggg catggaccag ccttcagatg
     1381 gcagaagtgg aagatgagcc tacttgtgag cgatgtgact ttaaggaaat gaagactggg
     1441 gaagaataat tagtgtttat aagacattta agaggccctt tttcatatac tgactcactg
     1501 atgaatcagc atttgcattt tatggaaaaa tataaatcca aagaaataat tta
//
