LOCUS       NM_031903                859 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein L32 (MRPL32),
            transcript variant 1, mRNA.
ACCESSION   NM_031903
VERSION     NM_031903.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 859)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 859)
  AUTHORS   Brown,A., Rathore,S., Kimanius,D., Aibara,S., Bai,X.C., Rorbach,J.,
            Amunts,A. and Ramakrishnan,V.
  TITLE     Structures of the human mitochondrial ribosome in native states of
            assembly
  JOURNAL   Nat Struct Mol Biol 24 (10), 866-869 (2017)
   PUBMED   28892042
REFERENCE   4  (bases 1 to 859)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   5  (bases 1 to 859)
  AUTHORS   Brown,A., Amunts,A., Bai,X.C., Sugimoto,Y., Edwards,P.C.,
            Murshudov,G., Scheres,S.H.W. and Ramakrishnan,V.
  TITLE     Structure of the large ribosomal subunit from human mitochondria
  JOURNAL   Science 346 (6210), 718-722 (2014)
   PUBMED   25278503
REFERENCE   6  (bases 1 to 859)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   7  (bases 1 to 859)
  AUTHORS   Bommel,H., Xie,G., Rossoll,W., Wiese,S., Jablonka,S., Boehm,T. and
            Sendtner,M.
  TITLE     Missense mutation in the tubulin-specific chaperone E (Tbce) gene
            in the mouse mutant progressive motor neuronopathy, a model of
            human motoneuron disease
  JOURNAL   J Cell Biol 159 (4), 563-569 (2002)
   PUBMED   12446740
REFERENCE   8  (bases 1 to 859)
  AUTHORS   Koc,E.C., Burkhart,W., Blackburn,K., Moyer,M.B., Schlatzer,D.M.,
            Moseley,A. and Spremulli,L.L.
  TITLE     The large subunit of the mammalian mitochondrial ribosome. Analysis
            of the complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (47), 43958-43969 (2001)
   PUBMED   11551941
REFERENCE   9  (bases 1 to 859)
  AUTHORS   Kenmochi,N., Suzuki,T., Uechi,T., Magoori,M., Kuniba,M., Higa,S.,
            Watanabe,K. and Tanaka,T.
  TITLE     The human mitochondrial ribosomal protein genes: mapping of 54
            genes to the chromosomes and implications for human disorders
  JOURNAL   Genomics 77 (1-2), 65-70 (2001)
   PUBMED   11543634
REFERENCE   10 (bases 1 to 859)
  AUTHORS   Suzuki,T., Terasaki,M., Takemoto-Hori,C., Hanada,T., Ueda,T.,
            Wada,A. and Watanabe,K.
  TITLE     Structural compensation for the deficit of rRNA with proteins in
            the mammalian mitochondrial ribosome. Systematic analysis of
            protein components of the large ribosomal subunit from mammalian
            mitochondria
  JOURNAL   J Biol Chem 276 (24), 21724-21736 (2001)
   PUBMED   11279069
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010132.5.
            
            On Nov 23, 2018 this sequence version replaced NM_031903.2.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 39S subunit protein that belongs to
            the L32 ribosomal protein family. A pseudogene corresponding to
            this gene is found on chromosome Xp. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.362047.1, AB049649.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000223324.3/
                                                  ENSP00000223324.2
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               AC010132.5         35173-35313         c
            142-323             AC010132.5         32553-32734         c
            324-859             AC010132.5         29832-30367         c
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p14.1"
     gene            1..859
                     /gene="MRPL32"
                     /gene_synonym="bL32m; bMRP-59b; HSPC283; L32mt; MRP-L32"
                     /note="mitochondrial ribosomal protein L32"
                     /db_xref="GeneID:64983"
                     /db_xref="HGNC:HGNC:14035"
                     /db_xref="MIM:611839"
     exon            1..141
                     /gene="MRPL32"
                     /gene_synonym="bL32m; bMRP-59b; HSPC283; L32mt; MRP-L32"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..578
                     /gene="MRPL32"
                     /gene_synonym="bL32m; bMRP-59b; HSPC283; L32mt; MRP-L32"
                     /EC_number="3.6.5.3"
                     /note="39S ribosomal protein L32, mitochondrial;
                     mitochondrial large ribosomal subunit protein bL32m"
                     /codon_start=1
                     /product="large ribosomal subunit protein bL32m precursor"
                     /protein_id="NP_114109.1"
                     /db_xref="CCDS:CCDS5468.1"
                     /db_xref="GeneID:64983"
                     /db_xref="HGNC:HGNC:14035"
                     /db_xref="MIM:611839"
                     /translation="MALAMLVLVVSPWSAARGVLRNYWERLLRKLPQSRPGFPSPPWG
                     PALAVQGPAMFTEPANDTSGSKENSSLLDSIFWMAAPKNRRTIEVNRCRRRNPQKLIK
                     VKNNIDVCPECGHLKQKHVLCAYCYEKVCKETAEIRRQIGKQEGGPFKAPTIETVVLY
                     TGETPSEQDQGKRIIERDRKRPSWFTQN"
     exon            142..323
                     /gene="MRPL32"
                     /gene_synonym="bL32m; bMRP-59b; HSPC283; L32mt; MRP-L32"
                     /inference="alignment:Splign:2.1.0"
     exon            324..859
                     /gene="MRPL32"
                     /gene_synonym="bL32m; bMRP-59b; HSPC283; L32mt; MRP-L32"
                     /inference="alignment:Splign:2.1.0"
     regulatory      831..836
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPL32"
                     /gene_synonym="bL32m; bMRP-59b; HSPC283; L32mt; MRP-L32"
                     /note="hexamer: AATAAA"
     polyA_site      859
                     /gene="MRPL32"
                     /gene_synonym="bL32m; bMRP-59b; HSPC283; L32mt; MRP-L32"
                     /note="major polyA site"
ORIGIN      
        1 cagcagggaa aatggcgctg gccatgctgg tcttggtggt ttcgccgtgg tctgcggccc
       61 ggggagtgct tcgaaactac tgggagcgac tgctacggaa gcttccgcag agccggccgg
      121 gctttcccag tcctccgtgg ggaccagcat tagcagtaca gggcccagcc atgtttacag
      181 agccagcaaa tgataccagt ggaagtaaag agaattccag ccttttggac agtatctttt
      241 ggatggcagc tcccaaaaat agacgcacca ttgaagttaa ccggtgtagg agaagaaatc
      301 cgcagaagct tattaaagtt aagaacaaca tagacgtttg tcctgaatgt ggtcacctga
      361 aacagaaaca tgtcctttgt gcctactgct atgaaaaggt gtgcaaggag actgcagaaa
      421 tcagacgaca gatagggaag caagaagggg gcccttttaa ggctcccacc atagagactg
      481 tggtgctgta cacgggagag acaccgtctg aacaagatca gggcaagagg atcattgaac
      541 gagacagaaa gcgaccatcc tggttcaccc agaattgaca ccaaagatgt taaaagggta
      601 acttcacagt aaatcatttc tcctgaaata gaggaagatt ctttatgttg ttgtgcttgt
      661 ttttaaatca tcagtatagt ttaacacatt ctttctaagc agttttgtgt gggataattt
      721 gaagaatata ttatgagtaa actccgaaaa ttttgtttat ccaaaggctc aatggattat
      781 gtttctatta tatacaaggt tttaagtaaa cataaaattt ccagaacaaa aataaaaaat
      841 ttaaaattca tagcaaaaa
//
