LOCUS       NM_014180               3173 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein L22 (MRPL22),
            transcript variant 1, mRNA.
ACCESSION   NM_014180
VERSION     NM_014180.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Brown,A., Rathore,S., Kimanius,D., Aibara,S., Bai,X.C., Rorbach,J.,
            Amunts,A. and Ramakrishnan,V.
  TITLE     Structures of the human mitochondrial ribosome in native states of
            assembly
  JOURNAL   Nat Struct Mol Biol 24 (10), 866-869 (2017)
   PUBMED   28892042
REFERENCE   3  (bases 1 to 3173)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   4  (bases 1 to 3173)
  AUTHORS   Brown,A., Amunts,A., Bai,X.C., Sugimoto,Y., Edwards,P.C.,
            Murshudov,G., Scheres,S.H.W. and Ramakrishnan,V.
  TITLE     Structure of the large ribosomal subunit from human mitochondria
  JOURNAL   Science 346 (6210), 718-722 (2014)
   PUBMED   25278503
REFERENCE   5  (bases 1 to 3173)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (bases 1 to 3173)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   7  (bases 1 to 3173)
  AUTHORS   O'Brien,T.W.
  TITLE     Evolution of a protein-rich mitochondrial ribosome: implications
            for human genetic disease
  JOURNAL   Gene 286 (1), 73-79 (2002)
   PUBMED   11943462
REFERENCE   8  (bases 1 to 3173)
  AUTHORS   Koc,E.C., Burkhart,W., Blackburn,K., Moyer,M.B., Schlatzer,D.M.,
            Moseley,A. and Spremulli,L.L.
  TITLE     The large subunit of the mammalian mitochondrial ribosome. Analysis
            of the complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (47), 43958-43969 (2001)
   PUBMED   11551941
REFERENCE   9  (bases 1 to 3173)
  AUTHORS   Kenmochi,N., Suzuki,T., Uechi,T., Magoori,M., Kuniba,M., Higa,S.,
            Watanabe,K. and Tanaka,T.
  TITLE     The human mitochondrial ribosomal protein genes: mapping of 54
            genes to the chromosomes and implications for human disorders
  JOURNAL   Genomics 77 (1-2), 65-70 (2001)
   PUBMED   11543634
REFERENCE   10 (bases 1 to 3173)
  AUTHORS   Goldschmidt-Reisin,S., Kitakawa,M., Herfurth,E.,
            Wittmann-Liebold,B., Grohmann,L. and Graack,H.R.
  TITLE     Mammalian mitochondrial ribosomal proteins. N-terminal amino acid
            sequencing, characterization, and identification of corresponding
            gene sequences
  JOURNAL   J Biol Chem 273 (52), 34828-34836 (1998)
   PUBMED   9857009
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP372420.1, AK000601.1,
            AC008410.6 and CR933679.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014180.3.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 39S subunit protein that belongs to
            the L22 ribosomal protein family. A pseudogene corresponding to
            this gene is found on chromosome 4q. Two transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.267279.1,
                                           SRR1803611.38860.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000523037.6/
                                                  ENSP00000431040.1
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                BP372420.1         1-13
            14-733              AK000601.1         1-720
            734-2754            AC008410.6         104973-106993       c
            2755-3173           CR933679.1         2616-3034
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.2"
     gene            1..3173
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /note="mitochondrial ribosomal protein L22"
                     /db_xref="GeneID:29093"
                     /db_xref="HGNC:HGNC:14480"
                     /db_xref="MIM:611835"
     exon            1..66
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..659
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /note="isoform a is encoded by transcript variant 1; 39S
                     ribosomal protein L22, mitochondrial; L25mt; 39S ribosomal
                     protein L25, mitochondrial; mitochondrial large ribosomal
                     subunit protein uL22m"
                     /codon_start=1
                     /product="large ribosomal subunit protein uL22m isoform a"
                     /protein_id="NP_054899.2"
                     /db_xref="CCDS:CCDS4331.1"
                     /db_xref="GeneID:29093"
                     /db_xref="HGNC:HGNC:14480"
                     /db_xref="MIM:611835"
                     /translation="MAAAVLGQLGALWIHNLRSRGKLALGVLPQSYIHTSASLDISRK
                     WEKKNKIVYPPQLPGEPRRPAEIYHCRRQIKYSKDKMWYLAKLIRGMSIDQALAQLEF
                     NDKKGAKIIKEVLLEAQDMAVRDHNVEFRSNLYIAESTSGRGQCLKRIRYHGRGRFGI
                     MEKVYCHYFVKLVEGPPPPPEPPKTAVAHAKEYIQQLRSRTIVHTL"
     exon            67..115
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /inference="alignment:Splign:2.1.0"
     exon            116..233
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /inference="alignment:Splign:2.1.0"
     exon            234..299
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /inference="alignment:Splign:2.1.0"
     exon            300..377
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /inference="alignment:Splign:2.1.0"
     exon            378..447
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /inference="alignment:Splign:2.1.0"
     exon            448..3173
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /inference="alignment:Splign:2.1.0"
     regulatory      715..720
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /note="hexamer: AACAAA"
     polyA_site      733
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /note="major polyA site"
     regulatory      984..989
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /note="hexamer: AATACA"
     polyA_site      1008
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
     regulatory      3148..3153
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
                     /note="hexamer: ATTAAA"
     polyA_site      3173
                     /gene="MRPL22"
                     /gene_synonym="HSPC158; L22mt; MRP-L22; MRP-L25; RPML25;
                     uL22m"
ORIGIN      
        1 gcttgaactc ggcggcttcc gtagcgggag ggcgaaagat ggcggcggca gtactgggac
       61 agttgggtgc gttatggata cataacctga ggagccgggg gaagctggcc ttgggtgttt
      121 tacctcaatc atatatccac acaagtgctt ctcttgacat ttctcgaaaa tgggagaaga
      181 agaataaaat tgtttatcct ccacaactgc ctggagaacc tcggagacca gcagaaatct
      241 accactgtcg aagacaaata aaatatagca aagacaagat gtggtatttg gcaaaattga
      301 tacgaggaat gtctattgac caggctttgg ctcagttgga attcaatgac aaaaaagggg
      361 ccaaaataat taaagaggtt ctcttagaag cacaagatat ggcagtgaga gaccataacg
      421 tggaattcag gtccaattta tatatagctg agtccacctc aggacgaggc cagtgcctga
      481 aacgcatccg ctaccatggc agaggtcgct ttgggatcat ggagaaggtt tattgccatt
      541 attttgtgaa gttggtggaa gggcccccac ctccacctga gccaccaaag acggcagttg
      601 cccatgccaa agagtatatt cagcagcttc gcagccggac catcgttcac actctatgat
      661 gaggagattc agactccaca gtgtatatat tttgccattt attttctaaa aataaacaaa
      721 aattgaaggc aaatgctttt tatgatttct cattgaatta ttgaaacagt gtataactgc
      781 tagttgtaaa tgaatattta taaggctttg cccatttctt ttgagcctct gggcatattt
      841 gtatgcattt gcgacttgga aggggaaatc agctttaaac atagtaacag actatatttc
      901 aaaaggcttt tggagtaact ttgaaaggga atgttaactt tccaactagt gtcctgcagg
      961 tggctgtttt gatactggac ttgaatacag tgttatgagg taagaaaatt gcttttattt
     1021 cacttaattt tctcttggga ccatgggaag ttagagtggc tgcatgttgt ttcattacag
     1081 tattttaggg tgttcagtgg taccgttttt cctttcactg ttggcacagg ccagtgagtc
     1141 aaaaattata ttttccttta ctgttttagc agtaactcga aactacagga gtttattaga
     1201 tatacgaatc taaaactcca aaaaccttta actttgaaaa ttttcttttg gttaagcaaa
     1261 gcctgttttt atttcttctt ccaacagtgc tttatattgt ttggatatct tgtttaggat
     1321 tgatttattt ttcagggtaa attcaattac tgagagagga attgaaccaa tctcagcatt
     1381 gcattctaaa atggggcagt aggtggcact caacctttta ttagcagaaa attgttagtt
     1441 tcaaattaga gtgttcaaca ctgcttccct ctcattcata cacgtcttca ttaagaagaa
     1501 gtactctttg tctacagccc tagtccaact tttgcatgca aaagaagaat cgaggatgct
     1561 tgcttgttaa tgcagacttc tgggccccac cccaatgatt caattcacta ggtcttatgg
     1621 ggggctggtt tcagaaatct atgttttaaa caaacacttc caggtaattc tgttgtaggg
     1681 gactacgctt tgagaatacg gctttaggag aatgaaaaaa tgtagtttta ttgccatgtt
     1741 ttaagatggc attaataata gttactgttt actgggaact tgttatttgc aagctctgtg
     1801 ctagtatttt acataattct tcacaacatc ctgaaaatgt agcttttgtt ttaccacttt
     1861 acaaaggaat aaaccgaagc acacagaatt caaataacta tcacaagtca cacagcatgt
     1921 aaatattggg gctatgattg aaaggtttct aaagtagctg gttctgcata ccttttgagt
     1981 atacagctgg gtttttgtgt ctctccagtt aacctaatgc atgggtaaaa agacatcagg
     2041 ggtcagtgga ataaaccaag gtgtaggaca gattcattct gggtcctaag ctgttgtagc
     2101 atggagtcag ggaaataaca ctggaatatt aaaaggtaac cttcattcta gtctgtgcat
     2161 tctcattagc cagaaagaag actggatagg tctcttccct tgctggcctc catttcattc
     2221 atttatgaaa caagagactc aatgttctct gtagaaaacc ttttttagaa tcacttgaag
     2281 aatttgttta aaaatgcaaa ttcctgggct ctactctcag actaagtgaa tcagaagctt
     2341 tggtgtggga gaccatggaa cctgcagttt taataaatgc cccagtgatt tgcatgttgg
     2401 agaattatca caccaggtca ctgtgctctc atgtggtggg gatgctccac gattgctctt
     2461 ggagttgtga cttccttggg tgtgactaaa gccaggtggt gccatggaca tcataaatat
     2521 aaattatgta agtgaaggca ctgcttcagt caggctgtaa aagctatttt gaaagtggta
     2581 aggaaaaagt aggattgtaa tgaagaaaat ttccttccca ggttaagcct tcatttggaa
     2641 ctgcttccag tacgttacaa aaagtaataa tagctaacct gtgtcactga ctgtatctgg
     2701 tactatttta agcactatat atacattatc tcaatgaatc tttcccattg tgacatccgg
     2761 ctagttttcg tggctaagag tgtactttct aacatccatg aacattgtgg tccccacctg
     2821 aagtttcact ttgcatgttc agtgatatgg tttggctctg tgttcccacc caatcttatc
     2881 tcaaattgta atccccatgt gtccagggag ggacctgtaa tccccaccta gtgacggagg
     2941 gaggtgattg atgatgggtg tggtgtaccc catgctgttc tcgtgatagt gagtgggttc
     3001 tcatgagatc tgatggtttt ataagtgttt gaaagttccc tcttcactcg ctgtcttgcc
     3061 tgctgccatg taaaatgtgc ctacttcacc ctctgccatg attgtaagtt tcctgaggcc
     3121 tccccagcca tgcagaactt tgacacaatt aaatgtcttt tctttataaa tta
//
