LOCUS       NM_017971                700 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein L20 (MRPL20),
            transcript variant 1, mRNA.
ACCESSION   NM_017971 XM_001125910 XM_930786 XM_930809
VERSION     NM_017971.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 700)
  AUTHORS   Brown,A., Rathore,S., Kimanius,D., Aibara,S., Bai,X.C., Rorbach,J.,
            Amunts,A. and Ramakrishnan,V.
  TITLE     Structures of the human mitochondrial ribosome in native states of
            assembly
  JOURNAL   Nat Struct Mol Biol 24 (10), 866-869 (2017)
   PUBMED   28892042
REFERENCE   3  (bases 1 to 700)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   4  (bases 1 to 700)
  AUTHORS   Brown,A., Amunts,A., Bai,X.C., Sugimoto,Y., Edwards,P.C.,
            Murshudov,G., Scheres,S.H.W. and Ramakrishnan,V.
  TITLE     Structure of the large ribosomal subunit from human mitochondria
  JOURNAL   Science 346 (6210), 718-722 (2014)
   PUBMED   25278503
REFERENCE   5  (bases 1 to 700)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (bases 1 to 700)
  AUTHORS   Stelzl,U., Worm,U., Lalowski,M., Haenig,C., Brembeck,F.H.,
            Goehler,H., Stroedicke,M., Zenkner,M., Schoenherr,A., Koeppen,S.,
            Timm,J., Mintzlaff,S., Abraham,C., Bock,N., Kietzmann,S.,
            Goedde,A., Toksoz,E., Droege,A., Krobitsch,S., Korn,B.,
            Birchmeier,W., Lehrach,H. and Wanker,E.E.
  TITLE     A human protein-protein interaction network: a resource for
            annotating the proteome
  JOURNAL   Cell 122 (6), 957-968 (2005)
   PUBMED   16169070
REFERENCE   7  (bases 1 to 700)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   8  (bases 1 to 700)
  AUTHORS   Koc,E.C., Burkhart,W., Blackburn,K., Moyer,M.B., Schlatzer,D.M.,
            Moseley,A. and Spremulli,L.L.
  TITLE     The large subunit of the mammalian mitochondrial ribosome. Analysis
            of the complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (47), 43958-43969 (2001)
   PUBMED   11551941
REFERENCE   9  (bases 1 to 700)
  AUTHORS   Kenmochi,N., Suzuki,T., Uechi,T., Magoori,M., Kuniba,M., Higa,S.,
            Watanabe,K. and Tanaka,T.
  TITLE     The human mitochondrial ribosomal protein genes: mapping of 54
            genes to the chromosomes and implications for human disorders
  JOURNAL   Genomics 77 (1-2), 65-70 (2001)
   PUBMED   11543634
REFERENCE   10 (bases 1 to 700)
  AUTHORS   Suzuki,T., Terasaki,M., Takemoto-Hori,C., Hanada,T., Ueda,T.,
            Wada,A. and Watanabe,K.
  TITLE     Structural compensation for the deficit of rRNA with proteins in
            the mammalian mitochondrial ribosome. Systematic analysis of
            protein components of the large ribosomal subunit from mammalian
            mitochondria
  JOURNAL   J Biol Chem 276 (24), 21724-21736 (2001)
   PUBMED   11279069
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG700526.1, BC009515.1 and
            AW015403.1.
            
            On Nov 23, 2018 this sequence version replaced NM_017971.3.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 39S subunit protein. A pseudogene
            corresponding to this gene is found on chromosome 21q. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.386128.1,
                                           SRR1163658.400595.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2147920, SAMEA2149178
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000344843.12/
                                                  ENSP00000341082.7
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                BG700526.1         23-34
            13-654              BC009515.1         1-642
            655-700             AW015403.1         30-75               c
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..700
                     /gene="MRPL20"
                     /gene_synonym="bL20m; L20mt; MRP-L20"
                     /note="mitochondrial ribosomal protein L20"
                     /db_xref="GeneID:55052"
                     /db_xref="HGNC:HGNC:14478"
                     /db_xref="MIM:611833"
     exon            1..163
                     /gene="MRPL20"
                     /gene_synonym="bL20m; L20mt; MRP-L20"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..526
                     /gene="MRPL20"
                     /gene_synonym="bL20m; L20mt; MRP-L20"
                     /note="isoform 1 is encoded by transcript variant 1; 39S
                     ribosomal protein L20, mitochondrial; mitochondrial large
                     ribosomal subunit protein bL20m"
                     /codon_start=1
                     /product="large ribosomal subunit protein bL20m isoform 1"
                     /protein_id="NP_060441.2"
                     /db_xref="CCDS:CCDS26.1"
                     /db_xref="GeneID:55052"
                     /db_xref="HGNC:HGNC:14478"
                     /db_xref="MIM:611833"
                     /translation="MVFLTAQLWLRNRVTDRYFRIQEVLKHARHFRGRKNRCYRLAVR
                     TVIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPALIGNLVKCQVELNRKVL
                     ADLAIYEPKTFKSLAALASRRRHEGFAAALGDGKEPEGIFSRVVQYH"
     exon            164..274
                     /gene="MRPL20"
                     /gene_synonym="bL20m; L20mt; MRP-L20"
                     /inference="alignment:Splign:2.1.0"
     exon            275..352
                     /gene="MRPL20"
                     /gene_synonym="bL20m; L20mt; MRP-L20"
                     /inference="alignment:Splign:2.1.0"
     exon            353..700
                     /gene="MRPL20"
                     /gene_synonym="bL20m; L20mt; MRP-L20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      679..684
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPL20"
                     /gene_synonym="bL20m; L20mt; MRP-L20"
                     /note="hexamer: AATGAA"
     polyA_site      700
                     /gene="MRPL20"
                     /gene_synonym="bL20m; L20mt; MRP-L20"
                     /note="major polyA site"
ORIGIN      
        1 atttccgacc cgggcaagat ggcagcggcg ctgcgcgtgc gttgttgagt gttcgggacg
       61 ccggcctgca ggcgccatgg tcttcctcac cgcgcagctc tggctgcgga atcgcgtcac
      121 cgaccgctac tttcggatcc aggaggtgct gaagcacgcc aggcacttcc ggggaaggaa
      181 aaatcgctgc tacaggttgg cggtcagaac cgtgattcga gcctttgtga aatgcaccaa
      241 agcccgatac ctgaagaaaa agaacatgag gaccctctgg attaatcgaa ttacagctgc
      301 tagccaggaa catggactga agtatccagc gctcattggg aatttagtta agtgccaggt
      361 ggagctcaac aggaaagtcc tagcggatct ggccatctac gagccaaaga ctttcaaatc
      421 tttggctgcc ttggccagta ggaggcgaca cgaaggattt gctgctgcct tgggggatgg
      481 gaaggaacct gaaggcattt tttccagagt ggtgcagtac cactgaggac tgttgctgta
      541 ttgattagga aaagagacag agtaatttgc agtttgtttg atttatactt ttgtttatct
      601 acaacccaat aacagacatg agggatggcc ctgtctctct gggacagagc ctcacagatg
      661 atgtccatgt tttgtgtgaa tgaaactcaa acactcttca
//
