LOCUS       NM_014161                970 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein L18 (MRPL18),
            transcript variant 1, mRNA.
ACCESSION   NM_014161
VERSION     NM_014161.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 970)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 970)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 970)
  AUTHORS   Brown,A., Rathore,S., Kimanius,D., Aibara,S., Bai,X.C., Rorbach,J.,
            Amunts,A. and Ramakrishnan,V.
  TITLE     Structures of the human mitochondrial ribosome in native states of
            assembly
  JOURNAL   Nat Struct Mol Biol 24 (10), 866-869 (2017)
   PUBMED   28892042
REFERENCE   5  (bases 1 to 970)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 970)
  AUTHORS   Smirnov,A., Entelis,N., Martin,R.P. and Tarassov,I.
  TITLE     Biological significance of 5S rRNA import into human mitochondria:
            role of ribosomal protein MRP-L18
  JOURNAL   Genes Dev 25 (12), 1289-1305 (2011)
   PUBMED   21685364
REFERENCE   7  (bases 1 to 970)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   8  (bases 1 to 970)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Behal,A., Touchman,J.W.,
            Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of adult human lens for the NEIBank
            Project: over 2000 non-redundant transcripts, novel genes and
            splice variants
  JOURNAL   Mol Vis 8, 171-184 (2002)
   PUBMED   12107413
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 970)
  AUTHORS   Koc,E.C., Burkhart,W., Blackburn,K., Moyer,M.B., Schlatzer,D.M.,
            Moseley,A. and Spremulli,L.L.
  TITLE     The large subunit of the mammalian mitochondrial ribosome. Analysis
            of the complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (47), 43958-43969 (2001)
   PUBMED   11551941
REFERENCE   10 (bases 1 to 970)
  AUTHORS   Kenmochi,N., Suzuki,T., Uechi,T., Magoori,M., Kuniba,M., Higa,S.,
            Watanabe,K. and Tanaka,T.
  TITLE     The human mitochondrial ribosomal protein genes: mapping of 54
            genes to the chromosomes and implications for human disorders
  JOURNAL   Genomics 77 (1-2), 65-70 (2001)
   PUBMED   11543634
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU932963.1, AF161556.1 and
            BC001623.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014161.4.
            
            Summary: This nuclear gene encodes a protein component of the
            larger 39S subunit of mitochondrial ribosome. This protein may also
            aid in the import of nuclear-encoded 5S rRNA into mitochondria.
            Alternative splicing results in multiple transcript variants, most
            of which are not predicted to encode a protein. A pseudogene of
            this gene is found on chromosome 16. [provided by RefSeq, Jan
            2016].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.506051.1,
                                           SRR5189661.14037.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000367034.5/
                                                  ENSP00000356001.4
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-173               BU932963.1         11-183
            174-962             AF161556.1         173-961
            963-970             BC001623.1         955-962
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.3"
     gene            1..970
                     /gene="MRPL18"
                     /gene_synonym="HSPC071; L18mt; MRP-L18; uL18m"
                     /note="mitochondrial ribosomal protein L18"
                     /db_xref="GeneID:29074"
                     /db_xref="HGNC:HGNC:14477"
                     /db_xref="MIM:611831"
     exon            1..173
                     /gene="MRPL18"
                     /gene_synonym="HSPC071; L18mt; MRP-L18; uL18m"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..664
                     /gene="MRPL18"
                     /gene_synonym="HSPC071; L18mt; MRP-L18; uL18m"
                     /note="isoform 1 is encoded by transcript variant 1; 39S
                     ribosomal protein L18, mitochondrial; mitochondrial large
                     ribosomal subunit protein uL18m"
                     /codon_start=1
                     /product="large ribosomal subunit protein uL18m isoform 1"
                     /protein_id="NP_054880.2"
                     /db_xref="CCDS:CCDS5270.1"
                     /db_xref="GeneID:29074"
                     /db_xref="HGNC:HGNC:14477"
                     /db_xref="MIM:611831"
                     /translation="MALRSRFWGLFSVCRNPGCRFAALSTSSEPAAKPEVDPVENEAV
                     APEFTNRNPRNLELLSVARKERGWRTVFPSREFWHRLRVIRTQHHVEALVEHQNGKVV
                     VSASTREWAIKKHLYSTRNVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKR
                     LQSAMTEGGVVLREPQRIYE"
     exon            174..360
                     /gene="MRPL18"
                     /gene_synonym="HSPC071; L18mt; MRP-L18; uL18m"
                     /inference="alignment:Splign:2.1.0"
     exon            361..592
                     /gene="MRPL18"
                     /gene_synonym="HSPC071; L18mt; MRP-L18; uL18m"
                     /inference="alignment:Splign:2.1.0"
     exon            593..970
                     /gene="MRPL18"
                     /gene_synonym="HSPC071; L18mt; MRP-L18; uL18m"
                     /inference="alignment:Splign:2.1.0"
     regulatory      946..951
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPL18"
                     /gene_synonym="HSPC071; L18mt; MRP-L18; uL18m"
                     /note="hexamer: AATAAA"
     polyA_site      970
                     /gene="MRPL18"
                     /gene_synonym="HSPC071; L18mt; MRP-L18; uL18m"
                     /note="major polyA site"
ORIGIN      
        1 gagagtttgg ggatctacag cagccaaagg cttgtccctg actttatatg gctgctcctg
       61 gcgagcgact gagtcgtccg tgaggaaaaa gaggcgaggc ttttccgaga tcgtctcagc
      121 gatggcgctt cggtcgcggt tttgggggtt gttctcggtt tgcaggaacc ctgggtgcag
      181 gttcgcagcc ctgtcaacca gctccgagcc ggcagcgaaa cctgaagtgg accctgtgga
      241 aaatgaagct gtcgccccag aattcaccaa ccggaacccc cggaacctgg agcttttatc
      301 tgtagccagg aaagagcggg gctggcggac ggtgtttccc tcccgtgagt tctggcacag
      361 gttgcgagtt ataaggactc agcatcatgt agaagcactt gtggagcatc agaatggcaa
      421 ggttgtggtt tcggcctcca ctcgtgagtg ggctattaaa aagcaccttt atagtaccag
      481 aaatgtggtg gcttgtgaga gtataggacg agtgctggca cagagatgct tagaggcggg
      541 aatcaacttc atggtctacc aaccaacccc gtgggaggca gcctcagact cgatgaaacg
      601 actacaaagt gccatgacag aaggtggtgt ggttctacgg gaacctcaga gaatctatga
      661 ataaatggaa gcattaattg ttttgaacat gtaaatataa atctgtcagc cactacagcc
      721 atcaaaagag agcatctgga agaacagcca gcttggaagt tttacagcaa taatgttgca
      781 gtggaatatt atttgtagtt aaggtcatcc tcctcccctt tctgtttttt taaatcaaga
      841 actacgttct gcccctctct tgggcttcag aagcatctaa gaaaagcagt catcaattat
      901 aattaacttt caaagggcaa gtcagaagtt gtttataaat tacaaaataa aggcatatta
      961 tgaactctta
//
