LOCUS       NM_021126               1350 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens mercaptopyruvate sulfurtransferase (MPST), transcript
            variant 1, mRNA.
ACCESSION   NM_021126
VERSION     NM_021126.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Matyasova,K., Soltysova,A., Babula,P., Krizanova,O. and Liskova,V.
  TITLE     Role of the 3-mercaptopyruvate sulfurtransferase in
            colon/colorectal cancers
  JOURNAL   Eur J Cell Biol 103 (2), 151415 (2024)
   PUBMED   38631098
  REMARK    GeneRIF: Role of the 3-mercaptopyruvate sulfurtransferase in
            colon/colorectal cancers.
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Pedre,B., Talwar,D., Barayeu,U., Schilling,D., Luzarowski,M.,
            Sokolowski,M., Glatt,S. and Dick,T.P.
  TITLE     3-Mercaptopyruvate sulfur transferase is a protein persulfidase
  JOURNAL   Nat Chem Biol 19 (4), 507-517 (2023)
   PUBMED   36732619
  REMARK    GeneRIF: 3-Mercaptopyruvate sulfur transferase is a protein
            persulfidase.
REFERENCE   3  (bases 1 to 1350)
  AUTHORS   Zhang,J., Cen,L., Zhang,X., Tang,C., Chen,Y., Zhang,Y., Yu,M.,
            Lu,C., Li,M., Li,S., Lin,B., Zhang,T., Song,X., Yu,C., Wu,H. and
            Shen,Z.
  TITLE     MPST deficiency promotes intestinal epithelial cell apoptosis and
            aggravates inflammatory bowel disease via AKT
  JOURNAL   Redox Biol 56, 102469 (2022)
   PUBMED   36126419
  REMARK    GeneRIF: MPST deficiency promotes intestinal epithelial cell
            apoptosis and aggravates inflammatory bowel disease via AKT.
REFERENCE   4  (bases 1 to 1350)
  AUTHORS   Li,M., Song,X., Jin,Q., Chen,Y., Zhang,J., Gao,J., Cen,L., Lin,Y.,
            Xu,C., He,X., Li,Y. and Yu,C.
  TITLE     3-Mercaptopyruvate sulfurtransferase represses tumour progression
            and predicts prognosis in hepatocellular carcinoma
  JOURNAL   Liver Int 42 (5), 1173-1184 (2022)
   PUBMED   35243746
  REMARK    GeneRIF: 3-Mercaptopyruvate sulfurtransferase represses tumour
            progression and predicts prognosis in hepatocellular carcinoma.
REFERENCE   5  (bases 1 to 1350)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   6  (bases 1 to 1350)
  AUTHORS   Jurkowska,H., Placha,W., Nagahara,N. and Wrobel,M.
  TITLE     The expression and activity of cystathionine-gamma-lyase and
            3-mercaptopyruvate sulfurtransferase in human neoplastic cell lines
  JOURNAL   Amino Acids 41 (1), 151-158 (2011)
   PUBMED   20446008
  REMARK    GeneRIF: In all the investigated cell lines, the activity of MPST
            was higher than that of CST, which suggests that in these cells,
            the main pathway of sulfane sulfur formation is the MPST-catalyzed
            reaction.
REFERENCE   7  (bases 1 to 1350)
  AUTHORS   Krueger,K., Koch,K., Juhling,A., Tepel,M. and Scholze,A.
  TITLE     Low expression of thiosulfate sulfurtransferase (rhodanese)
            predicts mortality in hemodialysis patients
  JOURNAL   Clin Biochem 43 (1-2), 95-101 (2010)
   PUBMED   19695240
  REMARK    GeneRIF: Data suggest that impaired rhodanese expression is
            associated with increased whole cell reactive oxygen species as
            well as higher mitochondrial superoxide production and predicts
            mortality in hemodialysis patients.
REFERENCE   8  (bases 1 to 1350)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   9  (bases 1 to 1350)
  AUTHORS   Billaut-Laden,I., Rat,E., Allorge,D., Crunelle-Thibaut,A.,
            Cauffiez,C., Chevalier,D., Lo-Guidice,J.M. and Broly,F.
  TITLE     Evidence for a functional genetic polymorphism of the human
            mercaptopyruvate sulfurtransferase (MPST), a cyanide detoxification
            enzyme
  JOURNAL   Toxicol Lett 165 (2), 101-111 (2006)
   PUBMED   16545926
  REMARK    GeneRIF: This work is the first report of a functional genetic
            polymorphism affecting MPST and should help in investigation of
            disorders such as mercaptolactate-cysteine disulfiduria.
REFERENCE   10 (bases 1 to 1350)
  AUTHORS   Pallini,R., Guazzi,G.C., Cannella,C. and Cacace,M.G.
  TITLE     Cloning and sequence analysis of the human liver rhodanese:
            comparison with the bovine and chicken enzymes
  JOURNAL   Biochem Biophys Res Commun 180 (2), 887-893 (1991)
   PUBMED   1953758
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z73420.1 and BC016737.1.
            
            On Jun 4, 2020 this sequence version replaced NM_021126.7.
            
            Summary: This protein encoded by this gene catalyzes the transfer
            of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol
            compounds. It may be involved in cysteine degradation and cyanide
            detoxification. There is confusion in literature between this
            protein (mercaptopyruvate sulfurtransferase, MPST), which appears
            to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese,
            TST, GeneID:7263), which is a mitochondrial protein. Deficiency in
            MPST activity has been implicated in a rare inheritable disorder
            known as mercaptolactate-cysteine disulfiduria (MCDU).
            Alternatively spliced transcript variants encoding same or
            different isoforms have been identified for this gene. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016737.1, SRR14038191.1592128.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000429360.6/
                                                  ENSP00000411719.3
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression,
                                                  longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                Z73420.1           18782-18820
            40-1345             BC016737.1         5-1310
            1346-1350           Z73420.1           28851-28855
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.3"
     gene            1..1350
                     /gene="MPST"
                     /gene_synonym="MST; TST2; TUM1"
                     /note="mercaptopyruvate sulfurtransferase"
                     /db_xref="GeneID:4357"
                     /db_xref="HGNC:HGNC:7223"
                     /db_xref="MIM:602496"
     exon            1..131
                     /gene="MPST"
                     /gene_synonym="MST; TST2; TUM1"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1049
                     /gene="MPST"
                     /gene_synonym="MST; TST2; TUM1"
                     /EC_number="2.8.1.2"
                     /note="isoform 1 is encoded by transcript variant 1; human
                     liver rhodanese; 3-mercaptopyruvate sulfurtransferase;
                     tRNA thiouridin modification protein 1; testicular tissue
                     protein Li 200"
                     /codon_start=1
                     /product="3-mercaptopyruvate sulfurtransferase isoform 1"
                     /protein_id="NP_066949.2"
                     /db_xref="CCDS:CCDS46703.1"
                     /db_xref="GeneID:4357"
                     /db_xref="HGNC:HGNC:7223"
                     /db_xref="MIM:602496"
                     /translation="MAEPGSRESETRARSPSVAAMASPQLCRALVSAQWVAEALRAPR
                     AGQPLQLLDASWYLPKLGRDARREFEERHIPGAAFFDIDQCSDRTSPYDHMLPGAEHF
                     AEYAGRLGVGAATHVVIYDASDQGLYSAPRVWWMFRAFGHHAVSLLDGGLRHWLRQNL
                     PLSSGKSQPAPAEFRAQLDPAFIKTYEDIKENLESRRFQVVDSRATGRFRGTEPEPRD
                     GIEPGHIPGTVNIPFTDFLSQEGLEKSPEEIRHLFQEKKVDLSKPLVATCGSGVTACH
                     VALGAYLCGKPDVPIYDGSWVEWYMRARPEDVISEGRGKTH"
     exon            132..750
                     /gene="MPST"
                     /gene_synonym="MST; TST2; TUM1"
                     /inference="alignment:Splign:2.1.0"
     exon            751..1350
                     /gene="MPST"
                     /gene_synonym="MST; TST2; TUM1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1329..1334
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MPST"
                     /gene_synonym="MST; TST2; TUM1"
                     /note="hexamer: AATAAA"
     polyA_site      1350
                     /gene="MPST"
                     /gene_synonym="MST; TST2; TUM1"
                     /note="major polyA site"
ORIGIN      
        1 gtgccggagt ctcctccctt tggtccgctg caggttggtg gcgggaggag gggacagctg
       61 cgggcgcggg gagggggcgc cgcgccgcgg gggccatggc ggagccagga agccgggagt
      121 ccgagacccg ggcccgcagc ccgagtgtcg ccgccatggc ttcgccgcag ctctgccgcg
      181 cgctggtgtc ggcgcaatgg gtggcggagg cgctgcgggc cccgcgcgct gggcagcctc
      241 tgcagctgct ggacgcctcc tggtacctgc cgaagctggg gcgcgacgcg cgacgcgagt
      301 tcgaggagcg ccacatcccg ggcgccgctt tcttcgacat cgaccagtgc agcgaccgca
      361 cctcgcccta cgaccacatg ctgcccgggg ccgagcattt cgcggagtac gcaggccgcc
      421 tgggcgtggg cgcggccacc cacgtcgtga tctacgacgc cagcgaccag ggcctctact
      481 ccgccccgcg cgtctggtgg atgttccgcg ccttcggcca ccacgccgtg tcactgcttg
      541 atggcggcct ccgccactgg ctgcgccaga acctcccgct cagctccggc aagagccaac
      601 ctgctcccgc cgagttccgc gctcagctcg accccgcctt catcaagacc tacgaggaca
      661 tcaaggagaa cctggaatcc cggcgcttcc aggtggtgga ctcccgagcc actggcaggt
      721 tccgcggcac cgagcccgag ccccgagacg gcattgaacc tggccacatc ccaggtaccg
      781 tgaacatccc cttcacagac ttcctgagcc aggaggggct ggagaagagc cctgaggaga
      841 tccgccatct gttccaggag aagaaagtgg acctgtctaa gccactggtg gccacgtgtg
      901 gctctggcgt cacagcctgc cacgtggcac taggggccta cctctgcggc aagccagacg
      961 tgcccatcta cgatggctcc tgggtggagt ggtacatgcg cgcccggccc gaggatgtca
     1021 tctcagaggg ccgggggaag acccactgaa gctgggcagg acacaggcga gctcaggtga
     1081 tgccggccac cagcaatgcc tggcctggta gctccgcttc tgctttcacc aagagagtgt
     1141 ttcttcactc aactcaggtg gcatttgggg tgacatctca aaggccagga attccgttga
     1201 cttgttggct gccagtaggg gcgggaggaa aggcggaggc gagccctgga ggagggaggc
     1261 cacaactccg agctgcccac ctggtgctga gctggggccc cgcctccttt ctgttttatt
     1321 tttgaggaaa taaaataacc aagtgctaaa
//
