LOCUS       NM_001364716           15419 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens myosin phosphatase Rho interacting protein (MPRIP),
            transcript variant 3, mRNA.
ACCESSION   NM_001364716 XM_011523761
VERSION     NM_001364716.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Ding,C.Z., Wang,G.L., Jiang,G.Q., Wang,H.T., Liu,Y.Y., Zhang,H.L.,
            Sun,F. and Wei,L.
  TITLE     [circDDX17 targets miR-223-3p / RIP3 to regulate the proliferation
            and apoptosis of non-small cell lung cancer cells]
  JOURNAL   Zhonghua Zhong Liu Za Zhi 46 (3), 239-248 (2024)
   PUBMED   38494770
  REMARK    GeneRIF: [circDDX17 targets miR-223-3p / RIP3 to regulate the
            proliferation and apoptosis of non-small cell lung cancer cells].
REFERENCE   2  (bases 1 to 15419)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 15419)
  AUTHORS   Balaban,C., Sztacho,M., Antiga,L., Miladinovic,A., Harata,M. and
            Hozak,P.
  TITLE     PIP2-Effector Protein MPRIP Regulates RNA Polymerase II
            Condensation and Transcription
  JOURNAL   Biomolecules 13 (3), 426 (2023)
   PUBMED   36979361
  REMARK    GeneRIF: PIP2-Effector Protein MPRIP Regulates RNA Polymerase II
            Condensation and Transcription.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 15419)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 15419)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 15419)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   7  (bases 1 to 15419)
  AUTHORS   Koga,Y. and Ikebe,M.
  TITLE     p116Rip decreases myosin II phosphorylation by activating myosin
            light chain phosphatase and by inactivating RhoA
  JOURNAL   J Biol Chem 280 (6), 4983-4991 (2005)
   PUBMED   15545284
  REMARK    GeneRIF: p116Rip is an important regulatory component that controls
            the RhoA signaling pathway, thus regulating MLCP activity and
            myosin phosphorylation in cells
REFERENCE   8  (bases 1 to 15419)
  AUTHORS   Mulder,J., Ariaens,A., van den Boomen,D. and Moolenaar,W.H.
  TITLE     p116Rip targets myosin phosphatase to the actin cytoskeleton and is
            essential for RhoA/ROCK-regulated neuritogenesis
  JOURNAL   Mol Biol Cell 15 (12), 5516-5527 (2004)
   PUBMED   15469989
REFERENCE   9  (bases 1 to 15419)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   10 (bases 1 to 15419)
  AUTHORS   Surks,H.K., Richards,C.T. and Mendelsohn,M.E.
  TITLE     Myosin phosphatase-Rho interacting protein. A new member of the
            myosin phosphatase complex that directly binds RhoA
  JOURNAL   J Biol Chem 278 (51), 51484-51493 (2003)
   PUBMED   14506264
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104024.8, AC079111.7 and AC055811.20.
            
            On Jan 21, 2020 this sequence version replaced NM_001364716.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA1966682 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000651222.2/
                                                ENSP00000498253.1
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AC104024.8         1-31                c
            32-515              AC079111.7         33392-33875
            516-593             AC079111.7         66614-66691
            594-659             AC079111.7         68915-68980
            660-811             AC079111.7         117606-117757
            812-896             AC079111.7         122521-122605
            897-1128            AC079111.7         127123-127354
            1129-1642           AC079111.7         128820-129333
            1643-1781           AC079111.7         133531-133669
            1782-1895           AC079111.7         134460-134573
            1896-1952           AC079111.7         136940-136996
            1953-2021           AC079111.7         138223-138291
            2022-2111           AC079111.7         141048-141137
            2112-2221           AC079111.7         145210-145319
            2222-2792           AC079111.7         149336-149906
            2793-2909           AC079111.7         152144-152260
            2910-6716           AC079111.7         155013-158819
            6717-6864           AC079111.7         162622-162769
            6865-6982           AC055811.20        35113-35230
            6983-7142           AC055811.20        36331-36490
            7143-7262           AC055811.20        37708-37827
            7263-7349           AC055811.20        38841-38927
            7350-7512           AC055811.20        39665-39827
            7513-7598           AC055811.20        42418-42503
            7599-15419          AC055811.20        47238-55058
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p11.2"
     gene            1..15419
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /note="myosin phosphatase Rho interacting protein"
                     /db_xref="GeneID:23164"
                     /db_xref="HGNC:HGNC:30321"
                     /db_xref="MIM:612935"
     exon            1..515
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             393..7670
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /note="isoform 3 is encoded by transcript variant 3; Rho
                     interacting protein 3"
                     /codon_start=1
                     /product="myosin phosphatase Rho-interacting protein
                     isoform 3"
                     /protein_id="NP_001351645.2"
                     /db_xref="CCDS:CCDS92265.1"
                     /db_xref="GeneID:23164"
                     /db_xref="HGNC:HGNC:30321"
                     /db_xref="MIM:612935"
                     /translation="MSAAKENPCRKFQANIFNKSKCQNCFKPRESHLLNDEDLTQAKP
                     IYGGWLLLAPDGTDFDNPVHRSRKWQRRFFILYEHGLLRYALDEMPTTLPQGTINMNQ
                     CTDVVDGEGRTGQKFSLCILTPEKEHFIRAETKEIVSGWLEMLMVYPRTNKQNQKKKR
                     KVEPPTPQEPGPAKVAVTSSSSSSSSSSSIPSAEKVPTTKSTLWQEEMRTKDQPDGSS
                     LSPAQSPSQSQPPAASSLREPGLESKEEESAMSSDRMDCGRKVRVESGYFSLEKTKQD
                     LKAEEQQLPPPLSPPSPSTPNHRYSCPESPSQELGGPLPSPGPRLPHQMVCSISLSSL
                     DVASQPPAYVDSGSTRGRGTERLGSAFAFKASRQYATLADVPKAIRISHREAFQVERR
                     RLERRTRARSPGREEVARLFGNERRRSQVIEKFEALDIEKAEHMETNAVGPSPSSDTR
                     QGRSEKRAFPRKRDFTNEAPPAPLPDASASPLSPHRRAKSLDRRSTEPSVTPDLLNFK
                     KGWLTKQYEDGQWKKHWFVLADQSLRYYRDSVAEEAADLDGEIDLSACYDVTEYPVQR
                     NYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVHPTTAPDVTSSLPEEKNKSSCSFE
                     TCPRPTEKQEAELGEPDPEQKRSRARERRREGRSKTFDWAEFRPIQQALAQERVGGVG
                     PADTHEPLRPEAEPGELERERARRREERRKRFGMLDATDGPGTEDAALRMEVDRSPGL
                     PMSDLKTHNVHVEIEQRWHQVETTPLREEKQVPIAPVHLSSEDGGDRLSTHELTSLLE
                     KELEQSQKEASDLLEQNRLLQDQLRVALGREQSAREGYVLQTEVAASPSGAWQRLHRV
                     NQDLQSELEAQCQRQELITHQIQTLKRSYGEAKDTIRHHEAEIRSLQARLSNAAAELA
                     IKEQALAKLKGDLKREQGRVREQLEERQHSEAALSSQLRASEQKLKSAEALLLEKTQE
                     LRGLETQQALQRDRQKEVQRLQERIADLSQQLGASEQAQRLMEEKLQRNYELLLESCE
                     KEKQALLQNLKEVEDKASAYEDQLQGQAQQVETLQKEKLSATFEGSEQVHQLEEQLEA
                     REASVRRLAEHVQSLCDERDLLRQRFQELTERVATSDEDVAELREKLRRREADNQSLE
                     HSYQRVSSQLQSMHTLLREKEEELERIKEAHEKVLEKKEQDLNEALVKMVALGSSLEE
                     TEIKLQAKEEILRKFASESPKDMEEPRSTPEETERDGTLLPGQPVQATRAPLGLPHTR
                     LEDEDEDLGAPPGEEYGDGSPSREDSMVPPKSVEVLDREGHQQGTAKLDQGAPGVKRQ
                     RIRFSTIQCQRYIHPEGSEKTWTSSTSSDTSQDRSPSEESMSSEPAPSVLPATGDSDT
                     YLSIIHSLETKLYVTEEKLKDVTVRLESQQGQSREALLALHHQWAGTEAQLREQLRAS
                     LLQVGALASQLEQERQERARRVEGHVGELGDFQVKNSQALMCLENCREQLRSLPRASQ
                     EDEQDARAASLASVESALVSAIQALQHWPAPAHGGARAQLETGGTEENGKPASLQQCS
                     QSELTEQEQVRLLSDQIALEASLISQIADSLKNTTSDVSRMLHEISWSGQPPMESAGA
                     PVDTWARKVLVDGEFWSQVESLRKHLGTLGGEAVGASGDGQQSIPQGLAPILANATWV
                     RAELSFATQSVRESFHRRLQSIQETLRGTQTALRQHKCLLREILGAYQTPDFERVMQQ
                     VLEALRLPAGHEDGVQLSWDLSPLGEVLGRDSDSSQEPFDVSDQSPGAFVAIQEELAQ
                     QLKEKASLLEEIAAALPSLPPVESLRDCQKLLQVSQSLSYNTCLGGLGQYSSLLVQDA
                     IIQAQVCYASCRIRLEYEKELQLCKESWQTREPSCSEQAQAARALREEYEELLRKQKS
                     EYLDVIAIVERENAELKAKAAQLDHQQQCLEDAESKHSMSMFTLRGRYEEEIRCVVEQ
                     LTRTESTLQAERSRVLSQLDASVRDRQDMERHHGEQIQTLEDRFQLKVRELQTIHEEE
                     LRTLQEHYSQSLRCLQDTLCLHQGPHPKALPAPAPNWQATQGEADSMTGLRERIQELE
                     AQMDVMREELGHKDLEGDAATLREKYQRDLESLKATCERGFAAMEETHQKKIEDLQRQ
                     HQRELEKLREEKDRLLAEETAATISAIEAMKNAHREEMERELEKSQRSQISSVNSDVE
                     ALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAH
                     NQELNNRLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLRVKESEIQYLKQE
                     ISSLKDELQTALRDKKYASDKYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKS
                     PDSATVSGYDIMKSKSNPDFLKKDRSCVTRQLRNIRSKSLKEGLTVQERLKLFESRDL
                     KKD"
     exon            516..593
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            594..659
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            660..811
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            812..896
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1128
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1642
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1781
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1895
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..1952
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2021
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2111
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2112..2221
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2222..2792
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2793..2909
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..6716
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            6717..6864
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            6865..6982
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            6983..7142
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            7143..7262
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            7263..7349
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            7350..7512
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            7513..7598
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     exon            7599..15419
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8319..8324
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /note="hexamer: ATTAAA"
     polyA_site      8336
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /note="major polyA site"
     regulatory      15398..15403
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
                     /note="hexamer: AATAAA"
     polyA_site      15419
                     /gene="MPRIP"
                     /gene_synonym="M-RIP; MRIP; p116Rip; RHOIP3; RIP3"
ORIGIN      
        1 gtagtgcgtg aggcgctccg gtcccatttg cagcggccgc ggggcgccga gggcagctgc
       61 ggcggcgcgg acgagccggg acggcggcga ccggagcctg agaggcgggc cgcagcggga
      121 agcagcgggc ccgaggccgc gtccatgggc ccgcggcggc cgggcggcgg cggtgcgggc
      181 gggagctggg cgtcgcgcgc gctgtgaggc ccgggcggct caggagcctc ggcgcgtgca
      241 gcgaaccgcc cgccgggaag gaggccgggc cgggcctgcg gcggggccgg cgagggatgc
      301 ggcgcggccg cgctgagccc ctagcccgcc gggagcgcca ggccggccag gcctgcgccg
      361 ccgccgccgc cgccgtcgcc gccgcgccga ccatgtcggc agccaaggag aacccgtgca
      421 ggaaattcca ggccaacatc ttcaacaaga gcaagtgtca gaactgcttc aagccccgcg
      481 agtcgcatct gctcaacgac gaggacctga cgcaggcaaa acccatttat ggcggttggc
      541 tgctcctggc tccagatggg accgactttg acaacccagt gcaccggtct cggaaatggc
      601 agcgacggtt cttcatcctt tacgagcacg gcctcttgcg ctacgccctg gatgagatgc
      661 ccacgaccct tcctcagggc accatcaaca tgaaccagtg cacagatgtg gtggatgggg
      721 agggccgcac gggccagaag ttctccctgt gtattctgac gcctgagaag gagcatttca
      781 tccgggcgga gaccaaggag atcgtcagtg ggtggctgga gatgctcatg gtctatcccc
      841 ggaccaacaa gcagaatcag aagaagaaac ggaaagtgga gccccccaca ccacaggagc
      901 ctgggcctgc caaggtggct gttaccagca gcagcagcag cagcagcagc agcagcagca
      961 tccccagtgc tgagaaagtc cccaccacca agtccacact ctggcaggaa gaaatgagga
     1021 ccaaggacca gccagatggc agcagcctga gtccagctca gagtcccagc cagagccagc
     1081 ctcctgctgc cagctccctg cgggaacctg ggctagagag caaagaagag gagagcgcca
     1141 tgagtagcga ccgcatggac tgtggccgca aagtccgggt ggagagcggc tacttctctc
     1201 tggagaagac caaacaggac ttgaaggctg aagaacagca gctgcccccg ccgctctccc
     1261 ctcccagccc cagcaccccc aaccacaggt acagttgccc cgagtcgccc tcccaggagc
     1321 tcggtggtcc tcttccttcc ccaggtcctc gactccccca ccaaatggtc tgcagcatct
     1381 ccctcagctc cctggatgtg gccagccagc cacctgccta cgtggactct ggcagcacta
     1441 gggggcgggg gacagagaga ctggggagcg cctttgcctt taaagccagc aggcaatatg
     1501 ccaccctggc cgacgtccct aaggccatca ggatcagcca ccgagaagcc ttccaggtgg
     1561 agagaaggcg gctggagcgt agaactcggg cccggagccc tggcagggag gaggtggccc
     1621 gtctgtttgg caacgagcgg aggaggtccc aggtgattga aaagtttgag gccttggaca
     1681 ttgagaaggc agagcacatg gagaccaatg cagtggggcc ctcaccatcc agcgacacac
     1741 gccagggccg cagcgagaag agggcgttcc ctaggaagcg ggacttcacc aatgaagccc
     1801 ccccagctcc tctcccagac gcctcggctt cccccctgtc tccacaccga agagccaagt
     1861 cactggacag gaggtccacg gagccctccg tgacgcccga cctgctgaat ttcaagaaag
     1921 gctggctgac taagcagtat gaggacggcc agtggaagaa acactggttt gtcctcgccg
     1981 atcaaagcct gagatactac agggattcag tggctgagga ggcagccgac ttggatggag
     2041 aaattgactt gtccgcatgt tacgatgtca cagagtatcc agttcagaga aactatggct
     2101 tccagataca tacaaaggag ggcgagttta ccctgtcggc catgacatct gggattcggc
     2161 ggaactggat ccagaccatc atgaagcacg tgcacccgac cactgccccg gatgtgacca
     2221 gctcgttgcc agaggaaaaa aacaagagca gctgctcttt tgagacctgc ccgaggccta
     2281 ctgagaagca agaggcagag ctgggggagc cggaccctga gcagaagagg agccgcgcac
     2341 gggagcggag gcgagagggc cgctccaaga cctttgactg ggctgagttc cgtcccatcc
     2401 agcaggccct ggctcaggag cgggtgggcg gcgtggggcc tgctgacacc cacgagcccc
     2461 tgcgccctga ggcggagcct ggggagctgg agcgggagcg tgcacggagg cgggaggagc
     2521 gccgcaagcg cttcgggatg ctcgacgcca cagacgggcc aggcactgag gatgcagccc
     2581 tgcgcatgga ggtggaccgg agcccagggc tgcctatgag cgacctcaaa acgcataacg
     2641 tccacgtgga gattgagcag cggtggcatc aggtggagac cacacctctc cgggaagaga
     2701 agcaggtgcc catcgccccc gtccacctgt cttctgaaga tgggggtgac cggctctcca
     2761 cacacgagct gacctctctg ctcgagaagg agctggagca gagccagaag gaggcctcag
     2821 accttctgga gcagaaccgg ctcctgcagg accagctgag ggtggccctg ggccgggagc
     2881 agagcgcccg tgagggctac gtgctgcaga ctgaagtggc cgcctcccca tcgggtgcct
     2941 ggcagaggct ccatagagtc aaccaagacc ttcaaagtga gcttgaagcc cagtgccagc
     3001 gccaggagct gattacacac cagattcaga ccctgaagcg tagctatggg gaggccaagg
     3061 acacgatccg gcaccacgag gctgagatcc ggagccttca ggcacggctc agcaatgcgg
     3121 ccgctgagct agccatcaag gagcaggcgc tggccaagct caagggcgac ctgaagcggg
     3181 agcagggccg ggtccgcgag cagctggagg agcggcaaca cagcgaggcg gcgctgagca
     3241 gccagctgag ggctagcgag cagaagctca agagtgctga ggccctgctg ctggagaaga
     3301 cgcaggagct acggggcctg gagacacagc aggcgctgca gcgggaccgg cagaaggagg
     3361 tccagaggct gcaggagcgc attgccgacc tcagccagca actgggcgcc agtgagcagg
     3421 cgcagcggct gatggaggag aagctgcaga gaaactatga gctgctgctg gagagctgtg
     3481 agaaggagaa gcaggcattg ctgcagaacc taaaggaagt ggaagacaag gccagcgcct
     3541 atgaggacca gctgcagggt caggcacagc aggtggagac cctgcagaag gagaagctga
     3601 gcgccacttt cgagggcagt gagcaggtgc accagctgga ggagcagctg gaggcacgag
     3661 aggccagcgt gcgcaggctc gcagagcacg tgcagagcct ctgtgacgag cgggacctcc
     3721 tcagacagcg gttccaggag ctgacagagc gcgtggccac gtccgacgag gatgtggctg
     3781 agctccggga aaagctgagg agaagagagg ctgacaacca gagcctggag cactcctacc
     3841 agagggtctc cagccagctg cagagcatgc acactctgct gagagagaag gaggaagagc
     3901 tggagcgcat taaggaagca catgagaagg ttctggagaa gaaggagcag gacctcaatg
     3961 aggctttggt taaaatggtt gccttgggga gcagcttaga ggaaacagaa attaagctcc
     4021 aggcaaaaga agagatttta aggaaatttg caagtgaatc tccaaaggac atggaagagc
     4081 cacggagtac ccctgaagag acagaaaggg atggcacttt gctcccaggc caaccagtcc
     4141 aagccactag ggcacctcta ggcctcccac acacaaggct cgaggatgag gacgaggacc
     4201 tgggggctcc tccgggggaa gagtacggtg atggcagccc cagtagggaa gacagcatgg
     4261 tgcccccaaa gtcagtggaa gtgcttgaca gggagggcca tcagcagggc acagccaaac
     4321 tcgaccaagg ggcacctggt gttaaaaggc aaagaatccg gttctccaca atccagtgcc
     4381 aaagatacat tcaccccgaa gggtctgaga agacctggac cagcagcaca tcttccgaca
     4441 ccagccagga ccggtcaccc tcggaagaaa gcatgtcctc agagcctgca cccagtgtac
     4501 tgcctgcaac tggcgactct gacacgtacc tctccatcat ccactccctg gagaccaagc
     4561 tctacgtcac agaggaaaag ctcaaagacg tgaccgtgag gctggagagc cagcagggtc
     4621 agagccgtga ggcactgctc gcactgcacc accagtgggc gggcaccgag gcccagctgc
     4681 gtgagcagct ccgcgccagc ctgctccagg ttggcgcact ggcctcccag ctggagcagg
     4741 agaggcagga gagggccagg agggttgaag ggcatgttgg agagcttggg gacttccagg
     4801 tcaagaatag tcaggccctg atgtgcctgg aaaattgccg agaacaactg agatctctgc
     4861 ctagggccag ccaggaggat gagcaggacg cacgcgcagc ctccctggcc agtgtggaga
     4921 gtgcactcgt cagcgccatc caagccctgc agcactggcc ggccccagcc catggcgggg
     4981 cccgtgcaca gctggagaca ggtggcaccg aggagaatgg gaagcctgcc tccctgcagc
     5041 agtgctccca gtctgagttg acagagcagg agcaggtgag gcttctttct gaccagattg
     5101 ctctggaggc ctcgctgatc agccagatag cagattccct gaagaacaca acatcagatg
     5161 tctcccgaat gctccatgag atttcttggt caggacagcc accgatggaa tctgctgggg
     5221 cccccgtaga cacctgggcc aggaaggtcc tagtggatgg tgagttctgg agccaggttg
     5281 agtctctgag gaagcacttg gggacactgg gaggagaggc agtcggtgcc tcaggagacg
     5341 ggcagcaaag catcccacag ggcctggccc ccatcctggc caatgccaca tgggtcaggg
     5401 cagagctcag ctttgccaca cagtcagtga gggagtcgtt ccaccgcagg ctacagagca
     5461 tccaggagac cctgcggggc acccagacgg ccctgcggca gcacaaatgc ctgctgaggg
     5521 aaatcctggg agcctaccaa accccagact ttgaaagagt gatgcagcag gtcttggaag
     5581 ccctcaggct tccagcgggc catgaagatg gtgttcagct gtcctgggac ctgagcccct
     5641 taggagaagt cctgggccga gactcagaca gctctcagga gcccttcgat gtgtctgacc
     5701 agagccctgg ggcctttgtt gctattcagg aggagcttgc ccagcagctg aaggagaagg
     5761 ccagcctctt agaggagata gcggctgcct taccatctct gccacctgtg gaatcgctga
     5821 gagattgcca gaagcttctc caggtgtccc agagtctctc gtataacact tgtttgggag
     5881 gcctcggtca gtattcttca ttgttggttc aggatgccat tattcaggcc caggtttgct
     5941 atgcgtcctg cagaatccgg ctagaatatg agaaggagct ccagctctgc aaggagtcct
     6001 ggcaaacccg ggagccctcc tgctcagagc aggcacaggc agcccgggcc ctgagggagg
     6061 agtatgagga gcttctccgc aagcagaaga gcgagtacct ggatgtgatc gccattgttg
     6121 aaagggagaa tgcagagctc aaggccaagg ccgcccagct agaccatcag cagcagtgtc
     6181 tggaggatgc agagagcaag cacagcatga gcatgttcac cctgcggggc aggtatgagg
     6241 aggagattcg gtgtgtggtg gagcagctga ccaggaccga gagcacactg caggctgagc
     6301 gcagccgggt cctgagccag ctggatgcct cggtcagaga caggcaggac atggagaggc
     6361 atcatggtga gcagatacag accctggagg acaggttcca gctcaaggtc cgggagctgc
     6421 agacgatcca cgaggaggag ctgaggaccc tgcaggagca ctactcgcag agcctgaggt
     6481 gccttcagga caccctctgc ctccaccagg ggccacaccc caaggccctg ccagcccctg
     6541 cccccaactg gcaggccacc cagggagagg ctgactccat gacggggctg agggagcgca
     6601 tccaggagct ggaggcccag atggatgtca tgcgggagga gctgggacac aaggacctgg
     6661 agggcgacgc ggccacactg cgtgagaagt accagaggga cttggagagc cttaaggcca
     6721 cgtgcgagcg agggtttgca gcaatggaag aaacgcacca gaagaagatt gaagatctcc
     6781 agaggcagca ccagcgggag ctagagaaac ttcgagaaga gaaagaccgc ctcctagccg
     6841 aggagacagc ggccaccatc tcagccatcg aagccatgaa gaacgcccac cgggaggaaa
     6901 tggagcggga gctggagaag agccagcggt cccagatcag cagcgtcaac tcggatgttg
     6961 aggccctgcg gcgccagtac ctggaggagc tgcagtcggt gcagcgggaa ctggaggtcc
     7021 tctcggagca gtactcgcag aagtgcctgg agaatgccca tctggcccag gcgctggagg
     7081 ccgagcggca ggccctgcgg cagtgccagc gtgagaacca ggagctcaat gcccacaacc
     7141 aggagctgaa caaccgcctg gctgcagaga tcacacggtt gcggacgctg ctgactgggg
     7201 acggcggtgg ggaggccact gggtcacccc ttgcacaggg caaggatgcc tatgaactag
     7261 aggtcttatt gcgggtaaag gaatcggaaa tacagtacct gaaacaggag attagctccc
     7321 tcaaggatga gctgcagacg gcactgcggg acaagaagta cgcaagtgac aagtacaaag
     7381 acatctacac agagctcagc atcgcgaagg ctaaggctga ctgtgacatc agcaggttga
     7441 aggagcagct caaggctgca acggaagcac tgggggagaa gtcccctgac agtgccacgg
     7501 tgtccggata tgatataatg aaatctaaaa gcaaccctga cttcttgaag aaagacagat
     7561 cctgtgtcac ccggcaactc agaaacatca ggtccaagag tctgaaggaa ggcctgacgg
     7621 tgcaagaacg gttgaagctc tttgaatcca gggacttgaa gaaagactag gtgtgtccca
     7681 tccaagttga gcacgcgcct tccccagctt gcagcagcac accccaagcg ctgcttttca
     7741 cctgtacctt tgttttatta ttattattat tattgctgtt gttgtcatcg ttaactgtgg
     7801 gcatggaatg cgtgaggctg gcttctgggt tgtccacacc actctctgct gtgttgactt
     7861 cctgttgtct tcatcaaagc ttttttccgt ggtattctaa aattaggcca gcagtggggg
     7921 ctgggagggc atctgtgtta gtcctttcct ggctgtgacc cgccacactc actgtcagta
     7981 ttaaggccca gcagcctgtt gataagctac cctgtctcac catgtgctgg tgtggaaacg
     8041 gggcccagcc agcacgcctc aaggtagatg gaatccccac tggtcagaga aaaagctatg
     8101 cggacactcc agcttggcct gggtcacagc actgactcct cacccgctag tctggctgtt
     8161 aagaggagaa agtgcactgc cttccagccc aggaggagga cagcattttg tatttgttcc
     8221 actgatgcag cttagaacca cacccctgag agtcgtggca aacctttcac aacctggaaa
     8281 atgttgaaag caaccattcc tatttttgtt tgttttttat taaatcttgc acaaaatccc
     8341 cggcccctct ccttccttcc ttccttcctt cctccgctcg ttcctttctt ggtctccagt
     8401 aaccctggtc ttttcataac tgctcgagat tgttgacctg cagcccaggt ttcagactct
     8461 gattgcaaaa aacaaatgaa ttccccccag gaatcattca aaatggggga aggtttgggg
     8521 gtttgggttt tttttttacc ttttggaaaa gaaaccgtca cattgctttg gaaaaggttg
     8581 agaggagacc cctgttaagt caagaagaaa gtacagagga tgtcagaatc tgatgagaac
     8641 agcacattag tgtttattga gactccgatc ttaactctca tttaattaat ctgagctctg
     8701 aaaacctatc ttgcagcatt tatctttaaa agagcctggt taaagtaaac ctatactaac
     8761 aattttgctt tttctaacag tttgaggaag acctttttaa ccaccacaaa acattctatg
     8821 gcaattcttg aaaatctctt aaattggagt ctattatggc cccatgaaaa ccattaatcc
     8881 cattaagata gggagtataa acccctggct ggtggaacag gttctgctac tttaggagca
     8941 aggtggggtg tgagtagatg gttttcatgc caagaacatg ctttcacttt gtattcatgc
     9001 ttgtgttggt gtgatggtct ctgtgggtgg gtggatgctt tgggcgttga aatctagaaa
     9061 tcctgttgct cagtttctag atgaagtcat gagcaaggcc atcagtggag ctctggcccc
     9121 gcccccaatg tgcagaaggg ccgggagcaa ggcctggagt tttcatgtgt tttcagaccc
     9181 aggtttaggt gctctcttct cactgaaata actaagtgct ctccactggc atcgagccct
     9241 ttccacaagt ttttaaggct cttaacccac actttcactc ctctgctact agtcttcagt
     9301 gttgttaaca gcaagagaaa attgggtttg tttaaaaatc tacttctctg aggtggcaca
     9361 gttgcgtagc tgtagtccca gctactcagg aggctgaggt agtaggattg cttgagcccg
     9421 ggaggtcgag gctgcaatca gtcatgatcg tgtcactgca ctccagcctg ggtgacaaag
     9481 caaggcccca tatcagatat agatatactt atcagacccc ccctgaccat ttagattggc
     9541 agtgctttga gaaatgcact atgacctttc tgtgtcaatg ggaatataca gaaggaacat
     9601 tcgggacccc gctgtccccc acagcctcat tgttgtctcc aggacactgc tgggtcacac
     9661 gaatgctcca ggacagacag ggacctggag tgcatcagga tctgaccaga taggagtttt
     9721 tgcctcgtgt ctgggtgcta cgattttgtg ccgttctctg aggtccacca cctgcccttc
     9781 ctggcatggt ttccttcgtg accatccctg ctgcccctgg gggtggaccc cactggccct
     9841 tctgcagaca gctccctgcc ttctgccctc cagggggttc tggccagagt ccatgcttgg
     9901 agacaggatc atctgccttc agccctcaca gtgctttaaa ttaaagcaag tttgcccata
     9961 ggacaaaaga gcatttgatt cccttttttc tgtcacatat cccttgaggc tggacttcag
    10021 gaatcctgga aaattaatat gagtgcagca tgtgaggggt cagagacagg ccagcagggc
    10081 gtctgcattc ctccctgcca caggtctctc cccagaggct ggtttagtgt agggtattgc
    10141 caggaaacgg actgaggctg ctttgctaag agctcctgaa aatgccctgg gcctgtcctg
    10201 gcgtttctga agagccctca tacagggaca gccaccatct gggtcaagga agtctgggtt
    10261 ccctgctggt gggctccatc ctgcgatgga gtgaaccagg cgagaaagga tgacgatgtt
    10321 cttcatgttg cacctggaca tgccccagga acagagactt gcccaggtgg caacactggc
    10381 acagatgttg acggctgccc aactggtgcc acactgagca gggagccttg tgctgcacag
    10441 ggctgggccc tctctccagt ttccttcctg caggcatcca aataccctgg aagggattta
    10501 acccctgaat tccagaggga agaaagaaga acagtgaaga agtagaactg gtttctgtat
    10561 ggggagagga aagtcttagg gacagctgca ggcggggtct caggctgctc cttggcacca
    10621 gctacacagt agtgagcttt cccagcttta ccgatgagga agaagttcaa atagatagac
    10681 ttcagcattt taattatttt cctataaatg tatttatgtg tagtatgcta gcaccagcca
    10741 gtaagctgtg ccacacatat gaatgggaaa gcgaggcagt tgtgctcgtg tgagtttctg
    10801 caggcttgtg ggtaattacc ttgtgtgcac gcctgcacgt gcagaatagt cactttctgc
    10861 tggtcagttt ctttatccac ccatggtgcc ccagccccag gcaggtgtgg agaccagcat
    10921 ttcagaggac gcgctgtcca cagcctcccg ggtctgagtg gatcattggg caggggtgga
    10981 gacagtgcgc tgccctctga gctggaagcc tgtgcttcag ggagtcataa tgggcctgtg
    11041 ctaagtgggt gatgcagtgg acatcccagg gcgactagag gtggcagtat cgcgaatttg
    11101 caggtttatt gaacaagagg taacatcgga gaggatcttg ccttcggatt cagcaagtat
    11161 gaaggcagaa gagcatggag agcaaggccc cacagcctgc ttagtgagtt ggaaggccca
    11221 gcaagaacct gtttctgcag cagccaccag ctcccatcac cccttgaccc tccagctcat
    11281 gctggagaag agggaatttt ggctgtttaa agaacacagt tgtgaatctc agaatgtgcc
    11341 tgaaaggaat actgacagat aaggccggaa acaaaactga tggcttgaaa aacattttta
    11401 tggaatgtat ttactatcat tttgttttac tatagaggta gatgggactc ttaacttttg
    11461 ggtacatgga aacatgctga aaactgaaca caatcctgat catcactcct gcctggctgt
    11521 ctcctgggag gctgccgggt gccacggagc tgggacacag cagagcccgc taggtgttgc
    11581 agggccctgg aggccaaggc caccctgtgt ggggtccctg ttggcagcca ggtccctaca
    11641 caaacaagta atcctgtttg gcctcctagg ttttgcatat gacctgcagc ctaatttggg
    11701 gtgtagggga agctctgctg gcccctgctc ctttgtatgt tgggtgactt taatggctgg
    11761 ccacataccc ctttctccca gctactcatt cactgacttg ggtaagttct aagacagttc
    11821 gcacttagaa aagaatgtga cacatcaaca ttaacttttc ctgaaaagaa gagtttgcct
    11881 aacatggtcc taaagaagct tggaatttat aagactttcc tttataagat atagtggggg
    11941 tttttttggg tggagggggg ttgttttttg ttttttgttt tcaagacaga gtctcgctct
    12001 gttgtccagg ctggagtgta gtggcatgat ctcggctcac tgcaacctct gcctcccagg
    12061 ttcatgccat tctcctgcct cagcctcccg agtagctggg actacaggtg tctgccgcca
    12121 cgcctggcta atttttttgt atttttagta gagacggggt ttcaccatgt tggtcaggat
    12181 ggtctcgatt tcctgacctc gtgatccgcc tgtctcggcc tcccaaagtg ctgggattac
    12241 aggcgtgagc caccacgcct ggcctataag atacggtaaa aaaaaaaaac tgtgacccct
    12301 ttgtcactaa gggagaaaga aattaagtat tgtcaaagtt ctataaagaa tggaaatgta
    12361 tgatattata cttcaaagga atttgatgtt gaaattttaa agaaaatttg tcatgttgat
    12421 gagaagcttc actttcctgg gaacttcatt cgttttaggg catgagataa aagtcctggc
    12481 taggggagcc ataggtctgt tgtacaagga atttgctttc taaacaagtt gtaacttgcc
    12541 ctaaggtccc tgttggagca ctaagaggtg acacaggcca gagacaacgt ttcgtttccc
    12601 cttccctgca agctgggatc agccctgtgt tttctccttt cagctgaagt gagcgaaggt
    12661 tctcagtgct ggcaaaagag cccactttct aaaaggactt gggaagaaag ctgctgggaa
    12721 cttgcttatt aaaaagttcc ttagaattaa ggtatctacc cactgttttc gcacctttca
    12781 ccttcctggg ctttcctgcc ctccagcatt cttctctaga gaggttccta gcccgctcag
    12841 cgcgagcgtc tccagtaggt aatagcagct gaacgtgggt tttccacgga cttcaggctt
    12901 ggaggtgcca tatacaagca cacttcttcc ttcccctggc ttctccatgc caccacccac
    12961 tttaaagatg taaactcagt agatttttca tccagtgaac ggtcatcttc acatcgaaag
    13021 gtgaaggcca ccactgttct caatgccaag caacagaacg ttctgagatg gccgttcttc
    13081 cttgcacagc agctacggca ggttgttctg cagccacccc ttagaggggg ctcttcgttt
    13141 tacctttgta cagttcttgt gtttacacat ttgggccaaa cagctttcag caagggcatg
    13201 tgtccacagc tgatgggcag ttaagaacca gcctgagctg aaggctagta ataccgtgct
    13261 gtaggctctt taaaaggaaa gcctggcata aacccagcat ggaaaggaac attatcagtt
    13321 atctcaaatt ttgtctgcca gggacaagac cctgttcatt cttttgccct tttcagaact
    13381 gtgagcttca agtattcttg cttctctgta aagggaagac atctcccttc tctgaaatcc
    13441 ttcaacaaaa gaaaaggctc ttggcagggt aggggagtca gtagctcaac actagatcat
    13501 ccctagagat ggggcaagtt tctgtctgaa cacgtcttgg gtccgagtcc ttaggtgttc
    13561 ggatgcagta ctttgtgaat acttaagcta ctgcatgctt ggtgtagctt gcaatttctc
    13621 tgtatttaaa agcagctgtg tttattttct tcaaaataac ctgtatatta tttagagcaa
    13681 gcaatgtaaa tattactgag aagttactgc agggattttt gtgacagagt ttgtatgggt
    13741 ttttaaaaaa atcttagaca ccccttttta agatggggag aacagggttg actgcaccgt
    13801 tgaagcccgc ccagcattat aaggaaatgt ttttaatgac tgctgcatct ttgtaaaacg
    13861 tttggtcatc taacagatgg ttttaaagtg tacaatatcc aaaataacga tagccctgta
    13921 tccatacatt gtttcattga aagaattctc tattgcctct tcttggtaga gccagagtcc
    13981 ttaaggaaaa tcaggaaaat taagaaaatg atggtgccat cttgaccaga cttctgcaca
    14041 gtaatttaac gctatcctag ggagacttgg ttgaaggcac agttctggga tcagggtcta
    14101 aatgtgcagt ttctgagaac cttcaagacc actcactggg cagggctctg tggagcactg
    14161 gagctgtttg gattccccag ccctttggtc atatcctgga attccgtgga ggctgcagaa
    14221 cttagatgca gctgtttttt acagcaccta tttttgtcag attggtaagg aaacactgag
    14281 tcacagaata cttaagaatt ggagactcca gtaatgtagg atggcctgag aggacgtcca
    14341 agtcccaagg ggtggacacg gcatgttcct cgggcacagc ctcagtgggg gccttcccca
    14401 ggcgcagctc ggccacctga ggaaagggtg tttcggaggc gcagccacac acacagcgct
    14461 ggcagcctca cggtcacgcc catcactccc tgccccccac tgcccttgag aagttagtgg
    14521 tgtcacatcc ttagttttat agacagctag gaatagattg tgaagaacac tcagttcact
    14581 actgtgttac atttatatca caagcttcaa ttaaaatgga ttttaaagga ttttaggatt
    14641 tacctttagt attaacaacg tatctactga catactgtta ggattcaaaa ccagttaagt
    14701 ataagaatta cttcatgtgg ttttcctagg gtacaattta taaaaggtag aaagcatcca
    14761 agtggctcct caacaattac aattcttaat gatttttctc acagctgtgc ccttctgtca
    14821 gggtcagtgt caaaattcgt tatcaaaggc aaaacctact gtgccaagct ggggcgctat
    14881 atgtgaacgg agtggaaatg cttcagtcac ctctgccgca gcttgtgatt ccagcagttc
    14941 tcacaaacgt tctgtcacat gatgaaaaga agcagcttgt ataattccaa ctggtgtttc
    15001 atttctgttc taatgctaag tggtaacgct taacaaacag actaaaagct gtgtgcagaa
    15061 gaaagggctg aatgagtacc gcctccctag gttccagcac agcgctcggg tctaagaagt
    15121 agagccccgg ggtagggtgg gccatccact gtcaggccag tgtctcaaga aagcctgacc
    15181 agctgagctg ctgctttttt tttggggggg ggggggggag gggcgtcttg aggctttttt
    15241 tttttttaca aagttagttt gtgatcaacg attcactaca attgaagtgt tactttgtca
    15301 gaatatttat tcctttgtgt gacatgctag attccctgga tgtagctgat catttttatt
    15361 ttgtaaatat tacctaactt tacataaact atatcataat aaactatttt tgcatcacc
//
