LOCUS       NM_000250               3216 bp    mRNA    linear   PRI 10-DEC-2024
DEFINITION  Homo sapiens myeloperoxidase (MPO), mRNA.
ACCESSION   NM_000250
VERSION     NM_000250.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Zou,Z., Deng,Y., Zhang,Q., Long,Z., Zhu,W., Gao,P., Xu,Y., Zhou,Y.,
            Tao,Z. and Chen,J.
  TITLE     [The auxiliary diagnostic value of ECP and MPO expression in nasal
            secretions in different types of rhinitis]
  JOURNAL   Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 38 (4), 292-297
            (2024)
   PUBMED   38563171
  REMARK    GeneRIF: [The auxiliary diagnostic value of ECP and MPO expression
            in nasal secretions in different types of rhinitis].
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Nijakowski,K., Motylewska,B., Banasik,E., Rutkowski,R., Tsaryk,V.,
            Luczak,J., Korybalska,K., Witowski,J., Surdacka,A. and Eder,P.
  TITLE     Treatment regimens and disease activity could alter salivary
            myeloperoxidase levels in patients with inflammatory bowel diseases
  JOURNAL   Pol Arch Intern Med 134 (1) (2024)
   PUBMED   38164522
  REMARK    GeneRIF: Treatment regimens and disease activity could alter
            salivary myeloperoxidase levels in patients with inflammatory bowel
            diseases.
REFERENCE   3  (bases 1 to 3216)
  AUTHORS   Saed,G.M., Nawaz,A., Alvero,A.A., Harper,A.K. and Morris,R.T.
  TITLE     Monomeric myeloperoxidase is a specific biomarker for early-stage
            ovarian cancer
  JOURNAL   Biomarkers 28 (7), 663-671 (2023)
   PUBMED   37982229
  REMARK    GeneRIF: Monomeric myeloperoxidase is a specific biomarker for
            early-stage ovarian cancer.
REFERENCE   4  (bases 1 to 3216)
  AUTHORS   Mihalic,Z.N., Kloimbock,T., Cosic-Mujkanovic,N., Valadez-Cosmes,P.,
            Maitz,K., Kindler,O., Wadsack,C., Heinemann,A., Marsche,G.,
            Gauster,M., Pollheimer,J. and Kargl,J.
  TITLE     Myeloperoxidase enhances the migration and invasion of human
            choriocarcinoma JEG-3 cells
  JOURNAL   Redox Biol 67, 102885 (2023)
   PUBMED   37776707
  REMARK    GeneRIF: Myeloperoxidase enhances the migration and invasion of
            human choriocarcinoma JEG-3 cells.
REFERENCE   5  (bases 1 to 3216)
  AUTHORS   Nettersheim,F.S., Schluter,J.D., Kreuzberg,W., Mehrkens,D.,
            Grimm,S., Nemade,H., Braumann,S., Hof,A., Guthoff,H., Peters,V.,
            Hoyer,F.F., Kargapolova,Y., Lackmann,J.W., Muller,S.,
            Pallasch,C.P., Hallek,M., Sachinidis,A., Adam,M., Winkels,H.,
            Baldus,S., Geissen,S. and Mollenhauer,M.
  TITLE     Myeloperoxidase is a critical mediator of anthracycline-induced
            cardiomyopathy
  JOURNAL   Basic Res Cardiol 118 (1), 36 (2023)
   PUBMED   37656254
  REMARK    GeneRIF: Myeloperoxidase is a critical mediator of
            anthracycline-induced cardiomyopathy.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3216)
  AUTHORS   Taylor,K.L., Pohl,J. and Kinkade,J.M. Jr.
  TITLE     Unique autolytic cleavage of human myeloperoxidase. Implications
            for the involvement of active site MET409
  JOURNAL   J Biol Chem 267 (35), 25282-25288 (1992)
   PUBMED   1334087
REFERENCE   7  (bases 1 to 3216)
  AUTHORS   Zeng,J. and Fenna,R.E.
  TITLE     X-ray crystal structure of canine myeloperoxidase at 3 A resolution
  JOURNAL   J Mol Biol 226 (1), 185-207 (1992)
   PUBMED   1320128
REFERENCE   8  (bases 1 to 3216)
  AUTHORS   Srivastava,C.H., Rado,T.A., Bauerle,D. and Broxmeyer,H.E.
  TITLE     Regulation of human bone marrow lactoferrin and myeloperoxidase
            gene expression by tumor necrosis factor-alpha
  JOURNAL   J Immunol 146 (3), 1014-1019 (1991)
   PUBMED   1703177
REFERENCE   9  (bases 1 to 3216)
  AUTHORS   Yamada,M., Hur,S.J. and Toda,H.
  TITLE     Isolation and characterization of extracellular myeloperoxidase
            precursor in HL-60 cell cultures
  JOURNAL   Biochem Biophys Res Commun 166 (2), 852-859 (1990)
   PUBMED   2154223
REFERENCE   10 (bases 58 to 3215)
  AUTHORS   Hashinaka,K., Nishio,C., Hur,S.J., Sakiyama,F., Tsunasawa,S. and
            Yamada,M.
  TITLE     Multiple species of myeloperoxidase messenger RNAs produced by
            alternative splicing and differential polyadenylation
  JOURNAL   Biochemistry 27 (16), 5906-5914 (1988)
   PUBMED   2903767
REFERENCE   11 (bases 1 to 3216)
  AUTHORS   Morishita,K., Tsuchiya,M., Asano,S., Kaziro,Y. and Nagata,S.
  TITLE     Chromosomal gene structure of human myeloperoxidase and regulation
            of its expression by granulocyte colony-stimulating factor
  JOURNAL   J Biol Chem 262 (31), 15208-15213 (1987)
   PUBMED   2444596
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M19507.1 and AC004687.2.
            
            On May 17, 2019 this sequence version replaced NM_000250.1.
            
            Summary: Myeloperoxidase (MPO) is a heme protein synthesized during
            myeloid differentiation that constitutes the major component of
            neutrophil azurophilic granules. Produced as a single chain
            precursor, myeloperoxidase is subsequently cleaved into a light and
            heavy chain. The mature myeloperoxidase is a tetramer composed of 2
            light chains and 2 heavy chains. This enzyme produces hypohalous
            acids central to the microbicidal activity of neutrophils.
            [provided by RefSeq, Nov 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M19507.1, X04876.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2149004, SAMEA2154529
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000225275.4/
                                                  ENSP00000225275.3
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3215              M19507.1           1-3215
            3216-3216           AC004687.2         143726-143726
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q22"
     gene            1..3216
                     /gene="MPO"
                     /note="myeloperoxidase"
                     /db_xref="GeneID:4353"
                     /db_xref="HGNC:HGNC:7218"
                     /db_xref="MIM:606989"
     exon            1..331
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     CDS             178..2415
                     /gene="MPO"
                     /EC_number="1.11.2.2"
                     /codon_start=1
                     /product="myeloperoxidase precursor"
                     /protein_id="NP_000241.1"
                     /db_xref="CCDS:CCDS11604.1"
                     /db_xref="GeneID:4353"
                     /db_xref="HGNC:HGNC:7218"
                     /db_xref="MIM:606989"
                     /translation="MGVPFFSSLRCMVDLGPCWAGGLTAEMKLLLALAGLLAILATPQ
                     PSEGAAPAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLRSGSASPMELLSYF
                     KQPVAATRTAVRAADYLHVALDLLERKLRSLWRRPFNVTDVLTPAQLNVLSKSSGCAY
                     QDVGVTCPEQDKYRTITGMCNNRRSPTLGASNRAFVRWLPAEYEDGFSLPYGWTPGVK
                     RNGFPVALARAVSNEIVRFPTDQLTPDQERSLMFMQWGQLLDHDLDFTPEPAARASFV
                     TGVNCETSCVQQPPCFPLKIPPNDPRIKNQADCIPFFRSCPACPGSNITIRNQINALT
                     SFVDASMVYGSEEPLARNLRNMSNQLGLLAVNQRFQDNGRALLPFDNLHDDPCLLTNR
                     SARIPCFLAGDTRSSEMPELTSMHTLLLREHNRLATELKSLNPRWDGERLYQEARKIV
                     GAMVQIITYRDYLPLVLGPTAMRKYLPTYRSYNDSVDPRIANVFTNAFRYGHTLIQPF
                     MFRLDNRYQPMEPNPRVPLSRVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIAVD
                     EIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPETVGQLGTVLRNL
                     KLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWEN
                     EGVFSMQQRQALAQISLPRIICDNTGITTVSKNNIFMSNSYPRDFVNCSTLPALNLAS
                     WREAS"
     exon            332..425
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            426..601
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            602..725
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            726..855
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1062
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1381
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1542
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1798
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1969
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2207
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..3216
                     /gene="MPO"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3188..3193
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MPO"
                     /note="hexamer: AATAAA"
     polyA_site      3216
                     /gene="MPO"
                     /note="major polyA site"
ORIGIN      
        1 gacaatatca ggtgagctgt ggaggtgggg tccttggaag ctggatgaca gcagctggca
       61 aggggataag agagcagtga gcccctccct caaggaggtc tggctttatc catagacagg
      121 gccctctgag gtggggctga ggtacaaagg gggattgagc agcccaggag aagagagatg
      181 ggggttccct tcttctcttc tctcagatgc atggtggact taggaccttg ctgggctggg
      241 ggtctcactg cagagatgaa gctgcttctg gccctagcag ggctcctggc cattctggcc
      301 acgccccagc cctctgaagg tgctgctcca gctgtcctgg gggaggtgga cacctcgttg
      361 gtgctgagct ccatggagga ggccaagcag ctggtggaca aggcctacaa ggagcggcgg
      421 gaaagcatca agcagcggct tcgcagcggc tcagccagcc ccatggaact cctatcctac
      481 ttcaagcagc cggtggcagc caccaggacg gcggtgaggg ccgctgacta cctgcacgtg
      541 gctctagacc tgctggagag gaagctgcgg tccctgtggc gaaggccatt caatgtcact
      601 gatgtgctga cgcccgccca gctgaatgtg ttgtccaagt caagcggctg cgcctaccag
      661 gacgtggggg tgacttgccc ggagcaggac aaataccgca ccatcaccgg gatgtgcaac
      721 aacagacgca gccccacgct gggggcctcc aaccgtgcct ttgtgcgctg gctgccggcg
      781 gagtatgagg acggcttctc tcttccctac ggctggacgc ccggggtcaa gcgcaacggc
      841 ttcccggtgg ctctggctcg cgcggtctcc aacgagatcg tgcgcttccc cactgatcag
      901 ctgactccgg accaggagcg ctcactcatg ttcatgcaat ggggccagct gttggaccac
      961 gacctcgact tcacccctga gccggccgcc cgggcctcct tcgtcactgg cgtcaactgc
     1021 gagaccagct gcgttcagca gccgccctgc ttcccgctca agatcccgcc caatgacccc
     1081 cgcatcaaga accaagccga ctgcatcccg ttcttccgct cctgcccggc ttgccccggg
     1141 agcaacatca ccatccgcaa ccagatcaac gcgctcactt ccttcgtgga cgccagcatg
     1201 gtgtacggca gcgaggagcc cctggccagg aacctgcgca acatgtccaa ccagctgggg
     1261 ctgctggccg tcaaccagcg cttccaagac aacggccggg ccctgctgcc ctttgacaac
     1321 ctgcacgatg acccctgtct cctcaccaac cgctcagcgc gcatcccctg cttcctggca
     1381 ggggacaccc gttccagtga gatgcccgag ctcacctcca tgcacaccct cttacttcgg
     1441 gagcacaacc ggctggccac agagctcaag agcctgaacc ctaggtggga tggggagagg
     1501 ctctaccagg aagcccggaa gatcgtgggg gccatggtcc agatcatcac ttaccgggac
     1561 tacctgcccc tggtgctggg gccaacggcc atgaggaagt acctgcccac gtaccgttcc
     1621 tacaatgact cagtggaccc acgcatcgcc aacgtcttca ccaatgcctt ccgctacggc
     1681 cacaccctca tccaaccctt catgttccgc ctggacaatc ggtaccagcc catggaaccc
     1741 aacccccgtg tccccctcag cagggtcttt tttgcctcct ggagggtcgt gctggaaggt
     1801 ggcattgacc ccatcctccg gggcctcatg gccacccctg ccaagctgaa tcgtcagaac
     1861 caaattgcag tggatgagat ccgggagcga ttgtttgagc aggtcatgag gattgggctg
     1921 gacctgcctg ctctgaacat gcagcgcagc agggaccacg gcctcccagg atacaatgcc
     1981 tggaggcgct tctgtgggct cccgcagcct gaaactgtgg gccagctggg cacggtgctg
     2041 aggaacctga aattggcgag gaaactgatg gagcagtatg gcacgcccaa caacatcgac
     2101 atctggatgg gcggcgtgtc cgagcctctg aagcgcaaag gccgcgtggg cccactcctc
     2161 gcctgcatca tcggtaccca gttcaggaag ctccgggatg gtgatcggtt ttggtgggag
     2221 aacgagggtg tgttcagcat gcagcagcga caggccctgg cccagatctc attgccccgg
     2281 atcatctgcg acaacacagg catcaccacc gtgtctaaga acaacatctt catgtccaac
     2341 tcatatcccc gggactttgt caactgcagt acacttcctg cattgaacct ggcttcctgg
     2401 agggaagcct cctagaggcc aggtaagggg gtgcagcagt gaggggtata tctgggctgg
     2461 ccagttggaa ccacggagat ctccttgccc tagatgagcc cagccctgtt ctgggtgcag
     2521 ctgagaaaat gagtgactag acgttcattt gtgtgctcat gtatgtgcga agtatataaa
     2581 ttggcttttc atgcgtgtgt gttgtctgaa catggggagt gtttcatggg ttatgtgtat
     2641 gtgccattta tgtgagtgtg tgtttgtgct gatgagaata ctgagtatgt ggaaggcagc
     2701 agagcggact ggtgaggagc acagctcagg aactagactg cctgggttcc aatcctggct
     2761 ctgtggcttg ctagctatgt gaccttgagc aaattaccct ccttaaacaa gagttttctt
     2821 ccttgtaaat tacatctgtc atggtttctt ggagggccca cttgtatcct ctggttcttc
     2881 atttattgag cacctactac atgcaaggca ctgtactagg cgtgagaagc atatagaggc
     2941 aagaaagaga taccaagatg ccatctgtgt cctggttagc agagctggac cagtggtgcc
     3001 ttggagggat aagccagctg cagctgggct gtgtggttga cttatgggcc cagccagcca
     3061 ggctcaggcc atggctcccc tttttcttcc tcaccctgat ttcttgctta ttcactgaag
     3121 ttctcctgaa gaggaactgg gcctgttgcc ctttctgtac catttatttg ctcccaatgt
     3181 ttatgataat aaaggcaccg ctgatgggga cctcca
//
