LOCUS       NM_001015052            1036 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens N-methylpurine DNA glycosylase (MPG), transcript
            variant 2, mRNA.
ACCESSION   NM_001015052
VERSION     NM_001015052.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Liu,X., Liu,L., Wu,A., Huang,S., Xu,Z., Zhang,X., Li,Z., Li,H. and
            Dong,J.
  TITLE     Transformed astrocytes confer temozolomide resistance on
            glioblastoma via delivering ALKBH7 to enhance APNG expression after
            educating by glioblastoma stem cells-derived exosomes
  JOURNAL   CNS Neurosci Ther 30 (2), e14599 (2024)
   PUBMED   38332576
  REMARK    GeneRIF: Transformed astrocytes confer temozolomide resistance on
            glioblastoma via delivering ALKBH7 to enhance APNG expression after
            educating by glioblastoma stem cells-derived exosomes.
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 1036)
  AUTHORS   Hamad,S.H., Brinkman,M.C., Tsai,Y.H., Mellouk,N., Cross,K.,
            Jaspers,I., Clark,P.I. and Granville,C.A.
  TITLE     Pilot Study to Detect Genes Involved in DNA Damage and Cancer in
            Humans: Potential Biomarkers of Exposure to E-Cigarette Aerosols
  JOURNAL   Genes (Basel) 12 (3), 448 (2021)
   PUBMED   33809907
  REMARK    GeneRIF: Pilot Study to Detect Genes Involved in DNA Damage and
            Cancer in Humans: Potential Biomarkers of Exposure to E-Cigarette
            Aerosols.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1036)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1036)
  AUTHORS   Thelen,A.Z. and O'Brien,P.J.
  TITLE     Recognition of 1,N2-ethenoguanine by alkyladenine DNA glycosylase
            is restricted by a conserved active-site residue
  JOURNAL   J Biol Chem 295 (6), 1685-1693 (2020)
   PUBMED   31882538
  REMARK    GeneRIF: Recognition of 1,N (2)-ethenoguanine by alkyladenine DNA
            glycosylase is restricted by a conserved active-site residue.
REFERENCE   6  (bases 1 to 1036)
  AUTHORS   Saparbaev,M. and Laval,J.
  TITLE     Excision of hypoxanthine from DNA containing dIMP residues by the
            Escherichia coli, yeast, rat, and human alkylpurine DNA
            glycosylases
  JOURNAL   Proc Natl Acad Sci U S A 91 (13), 5873-5877 (1994)
   PUBMED   8016081
  REMARK    GeneRIF: The human alkyl-N-purine-DNA glycosylase (ANPG or MPG)
            excises with high efficiency hypoxanthine residues, deamination
            product of adenine, when present in DNA. ANPG is by far the most
            efficient hypoxanthine-DNA glycosylase of all the enzymes tested.
REFERENCE   7  (bases 1 to 1036)
  AUTHORS   Kielman,M.F., Smits,R., Devi,T.S., Fodde,R. and Bernini,L.F.
  TITLE     Homology of a 130-kb region enclosing the alpha-globin gene
            cluster, the alpha-locus controlling region, and two non-globin
            genes in human and mouse
  JOURNAL   Mamm Genome 4 (6), 314-323 (1993)
   PUBMED   8318735
REFERENCE   8  (bases 1 to 1036)
  AUTHORS   Samson,L., Derfler,B., Boosalis,M. and Call,K.
  TITLE     Cloning and characterization of a 3-methyladenine DNA glycosylase
            cDNA from human cells whose gene maps to chromosome 16
  JOURNAL   Proc Natl Acad Sci U S A 88 (20), 9127-9131 (1991)
   PUBMED   1924375
REFERENCE   9  (bases 1 to 1036)
  AUTHORS   Chakravarti,D., Ibeanu,G.C., Tano,K. and Mitra,S.
  TITLE     Cloning and expression in Escherichia coli of a human cDNA encoding
            the DNA repair protein N-methylpurine-DNA glycosylase
  JOURNAL   J Biol Chem 266 (24), 15710-15715 (1991)
   PUBMED   1874728
REFERENCE   10 (bases 1 to 1036)
  AUTHORS   O'Connor,T.R. and Laval,J.
  TITLE     Human cDNA expressing a functional DNA glycosylase excising
            3-methyladenine and 7-methylguanine
  JOURNAL   Biochem Biophys Res Commun 176 (3), 1170-1177 (1991)
   PUBMED   1645538
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM796301.1 and BC014991.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001015052.2.
            
            Transcript Variant: This variant (2) lacks an exon in the 5' region
            and initiates translation at an alternate start codon, compared to
            variant 1. The encoded isoform (b) is shorter and has a distinct
            N-terminus, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.264888.1,
                                           SRR7346977.427904.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356432.8/ ENSP00000348809.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 BM796301.1         56-61
            7-1036              BC014991.1         1-1030
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..1036
                     /gene="MPG"
                     /gene_synonym="AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1;
                     PIG11; PIG16"
                     /note="N-methylpurine DNA glycosylase"
                     /db_xref="GeneID:4350"
                     /db_xref="HGNC:HGNC:7211"
                     /db_xref="MIM:156565"
     exon            1..109
                     /gene="MPG"
                     /gene_synonym="AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1;
                     PIG11; PIG16"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..967
                     /gene="MPG"
                     /gene_synonym="AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1;
                     PIG11; PIG16"
                     /EC_number="3.2.2.21"
                     /note="isoform b is encoded by transcript variant 2;
                     proliferation-inducing protein 11; proliferation-inducing
                     protein 16; N-methylpurine-DNA glycosylase, MPG; 3' end of
                     the Mid1 gene, localized 68 kb upstream the humanzeta
                     globin gene on 16p; CRA36.1 (3-methyl-adenine DNA
                     glycosylase); 3-alkyladenine DNA glycosylase;
                     3-methyladenine DNA glycosidase"
                     /codon_start=1
                     /product="DNA-3-methyladenine glycosylase isoform b"
                     /protein_id="NP_001015052.1"
                     /db_xref="CCDS:CCDS32345.1"
                     /db_xref="GeneID:4350"
                     /db_xref="HGNC:HGNC:7211"
                     /db_xref="MIM:156565"
                     /translation="MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSS
                     DAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVL
                     VRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMY
                     FCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQ
                     ALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRG
                     SPWVSVVDRVAEQDTQA"
     exon            110..385
                     /gene="MPG"
                     /gene_synonym="AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1;
                     PIG11; PIG16"
                     /inference="alignment:Splign:2.1.0"
     exon            386..590
                     /gene="MPG"
                     /gene_synonym="AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1;
                     PIG11; PIG16"
                     /inference="alignment:Splign:2.1.0"
     exon            591..1036
                     /gene="MPG"
                     /gene_synonym="AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1;
                     PIG11; PIG16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1013..1018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MPG"
                     /gene_synonym="AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1;
                     PIG11; PIG16"
                     /note="hexamer: AATAAA"
     polyA_site      1036
                     /gene="MPG"
                     /gene_synonym="AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1;
                     PIG11; PIG16"
                     /note="major polyA site"
ORIGIN      
        1 ggtcctaggg gtgcttccgt ggtcggcggc tgctgggctc cgcgccgggg tccgagtccc
       61 acgaagcccc ggcccgagcc gccggatgcc cgcgcgcagc ggggcccagt tttgccgacg
      121 gatggggcaa aagaagcagc gaccagctag agcagggcag ccacacagct cgtccgacgc
      181 agcccaggca cctgcagagc agccacacag ctcgtccgat gcagcccagg caccttgccc
      241 cagggagcgc tgcttgggac cgcccaccac tccgggccca taccgcagca tctatttctc
      301 aagcccaaag ggccacctta cccgactggg gttggagttc ttcgaccagc cggcagtccc
      361 cctggcccgg gcatttctgg gacaggtcct agtccggcga cttcctaatg gcacagaact
      421 ccgaggccgc atcgtggaga ccgaggcata cctggggcca gaggatgaag ccgcccactc
      481 aaggggtggc cggcagaccc cccgcaaccg aggcatgttc atgaagccgg ggaccctgta
      541 cgtgtacatc atttacggca tgtacttctg catgaacatc tccagccagg gggacggggc
      601 ttgcgtcttg ctgcgagcac tggagcccct ggaaggtctg gagaccatgc gtcagcttcg
      661 cagcaccctc cggaaaggca ccgccagccg tgtcctcaag gaccgcgagc tctgcagtgg
      721 cccctccaag ctgtgccagg ccctggccat caacaagagc tttgaccaga gggacctggc
      781 acaggatgaa gctgtatggc tggagcgtgg tcccctggag cccagtgagc cggctgtagt
      841 ggcagcagcc cgggtgggcg tcggccatgc aggggagtgg gcccggaaac ccctccgctt
      901 ctatgtccgg ggcagcccct gggtcagtgt ggtcgacaga gtggctgagc aggacacaca
      961 ggcctgagca aagggcctgc ccagacaaga ttttttaatt gtttaaaaac cgaataaatg
     1021 ttttatttct agaaaa
//
