LOCUS       NM_001039396            4418 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens macrophage expressed 1 (MPEG1), mRNA.
ACCESSION   NM_001039396 XM_166227 XM_943258
VERSION     NM_001039396.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Rodriguez-Silvestre,P., Laub,M., Krawczyk,P.A., Davies,A.K.,
            Schessner,J.P., Parveen,R., Tuck,B.J., McEwan,W.A., Borner,G.H.H.
            and Kozik,P.
  TITLE     Perforin-2 is a pore-forming effector of endocytic escape in
            cross-presenting dendritic cells
  JOURNAL   Science 380 (6651), 1258-1265 (2023)
   PUBMED   37347855
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Pastar,I., Sawaya,A.P., Marjanovic,J., Burgess,J.L., Strbo,N.,
            Rivas,K.E., Wikramanayake,T.C., Head,C.R., Stone,R.C., Jozic,I.,
            Stojadinovic,O., Kornfeld,E.Y., Kirsner,R.S., Lev-Tov,H. and
            Tomic-Canic,M.
  TITLE     Intracellular Staphylococcus aureus triggers pyroptosis and
            contributes to inhibition of healing due to perforin-2 suppression
  JOURNAL   J Clin Invest 131 (24) (2021)
   PUBMED   34730110
  REMARK    GeneRIF: Intracellular Staphylococcus aureus triggers pyroptosis
            and contributes to inhibition of healing due to perforin-2
            suppression.
REFERENCE   3  (bases 1 to 4418)
  AUTHORS   Merselis,L.C., Rivas,Z.P. and Munson,G.P.
  TITLE     Breaching the Bacterial Envelope: The Pivotal Role of Perforin-2
            (MPEG1) Within Phagocytes
  JOURNAL   Front Immunol 12, 597951 (2021)
   PUBMED   33692780
  REMARK    GeneRIF: Breaching the Bacterial Envelope: The Pivotal Role of
            Perforin-2 (MPEG1) Within Phagocytes.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4418)
  AUTHORS   Merselis,L.C., Jiang,S.Y., Nelson,S.F., Lee,H., Prabaker,K.K.,
            Baker,J.L., Munson,G.P. and Butte,M.J.
  TITLE     MPEG1/Perforin-2 Haploinsufficiency Associated Polymicrobial Skin
            Infections and Considerations for Interferon-gamma Therapy
  JOURNAL   Front Immunol 11, 601584 (2020)
   PUBMED   33224153
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4418)
  AUTHORS   Kattel,S., Bhatt,H., Xu,S., Gurung,S., Pokharel,S. and Sharma,U.C.
  TITLE     Macrophage-specific protein perforin-2 is associated with poor
            neurological recovery and reduced survival after sudden cardiac
            arrest
  JOURNAL   Resuscitation 155, 180-188 (2020)
   PUBMED   32828820
  REMARK    GeneRIF: Macrophage-specific protein perforin-2 is associated with
            poor neurological recovery and reduced survival after sudden
            cardiac arrest.
REFERENCE   6  (bases 1 to 4418)
  AUTHORS   McCormack,R. and Podack,E.R.
  TITLE     Perforin-2/Mpeg1 and other pore-forming proteins throughout
            evolution
  JOURNAL   J Leukoc Biol 98 (5), 761-768 (2015)
   PUBMED   26307549
  REMARK    GeneRIF: the impact of Perforin-2/macrophage-expressed protein 1
            from the earliest multicellular organisms to modern vertebrates, as
            well as review the development of other membrane attack complexes
            of complement and Perforin domain member proteins
            Review article
REFERENCE   7  (bases 1 to 4418)
  AUTHORS   McCormack,R.M., de Armas,L.R., Shiratsuchi,M., Fiorentino,D.G.,
            Olsson,M.L., Lichtenheld,M.G., Morales,A., Lyapichev,K.,
            Gonzalez,L.E., Strbo,N., Sukumar,N., Stojadinovic,O., Plano,G.V.,
            Munson,G.P., Tomic-Canic,M., Kirsner,R.S., Russell,D.G. and
            Podack,E.R.
  TITLE     Perforin-2 is essential for intracellular defense of parenchymal
            cells and phagocytes against pathogenic bacteria
  JOURNAL   Elife 4, e06508 (2015)
   PUBMED   26402460
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4418)
  AUTHORS   Fields,K.A., McCormack,R., de Armas,L.R. and Podack,E.R.
  TITLE     Perforin-2 restricts growth of Chlamydia trachomatis in macrophages
  JOURNAL   Infect Immun 81 (8), 3045-3054 (2013)
   PUBMED   23753625
  REMARK    GeneRIF: Knockdown of perforin-2 in macrophages did not alter the
            invasion of host cells but did result in chlamydial growth that
            closely mirrored that detected in HeLa cells.
REFERENCE   9  (bases 1 to 4418)
  AUTHORS   McCormack,R., de Armas,L.R., Shiratsuchi,M., Ramos,J.E. and
            Podack,E.R.
  TITLE     Inhibition of intracellular bacterial replication in fibroblasts is
            dependent on the perforin-like protein (perforin-2) encoded by
            macrophage-expressed gene 1
  JOURNAL   J Innate Immun 5 (2), 185-194 (2013)
   PUBMED   23257510
REFERENCE   10 (bases 1 to 4418)
  AUTHORS   Spilsbury,K., O'Mara,M.A., Wu,W.M., Rowe,P.B., Symonds,G. and
            Takayama,Y.
  TITLE     Isolation of a novel macrophage-specific gene by differential cDNA
            analysis
  JOURNAL   Blood 85 (6), 1620-1629 (1995)
   PUBMED   7888681
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA946058.1, BC104997.1, AP002358.3 and AW205154.1.
            
            On May 17, 2019 this sequence version replaced NM_001039396.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: SRR1803616.267486.1, AK097881.1
                                        [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361050.4/ ENSP00000354335.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                DA946058.1         95-135
            42-2258             BC104997.1         1-2217
            2259-4034           AP002358.3         28443-30218         c
            4035-4418           AW205154.1         16-399              c
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.1"
     gene            1..4418
                     /gene="MPEG1"
                     /gene_synonym="IMD77; Mpg-1; MPG1; MPS1; P-2"
                     /note="macrophage expressed 1"
                     /db_xref="GeneID:219972"
                     /db_xref="HGNC:HGNC:29619"
                     /db_xref="MIM:610390"
     exon            1..4418
                     /gene="MPEG1"
                     /gene_synonym="IMD77; Mpg-1; MPG1; MPS1; P-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..2213
                     /gene="MPEG1"
                     /gene_synonym="IMD77; Mpg-1; MPG1; MPS1; P-2"
                     /note="macrophage expressed gene 1; macrophage gene 1
                     protein; macrophage-expressed gene 1 protein; perforin 2"
                     /codon_start=1
                     /product="macrophage-expressed gene 1 protein precursor"
                     /protein_id="NP_001034485.1"
                     /db_xref="CCDS:CCDS41650.1"
                     /db_xref="GeneID:219972"
                     /db_xref="HGNC:HGNC:29619"
                     /db_xref="MIM:610390"
                     /translation="MNNFRATILFWAAAAWAKSGKPSGEMDEVGVQKCKNALKLPVLE
                     VLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILE
                     SWANYQSSTSYSINTELSLFSKVNGKFSTEFQRMKTLQVKDQAITTRVQVRNLVYTVK
                     INPTLELSSGFRKELLDISDRLENNQTRMATYLAELLVLNYGTHVTTSVDAGAALIQE
                     DHLRASFLQDSQSSRSAVTASAGLAFQNTVNFKFEENYTSQNVLTKSYLSNRTNSRVQ
                     SIGGVPFYPGITLQAWQQGITNHLVAIDRSGLPLHFFINPNMLPDLPGPLVKKVSKTV
                     ETAVKRYYTFNTYPGCTDLNSPNFNFQANTDDGSCEGKMTNFSFGGVYQECTQLSGNR
                     DVLLCQKLEQKNPLTGDFSCPSGYSPVHLLSQIHEEGYNHLECHRKCTLLVFCKTVCE
                     DVFQVAKAEFRAFWCVASSQVPENSGLLFGGLFSSKSINPMTNAQSCPAGYFPLRLFE
                     NLKVCVSQDYELGSRFAVPFGGFFSCTVGNPLVDPAISRDLGAPSLKKCPGGFSQHPA
                     LISDGCQVSYCVKSGLFTGGSLPPARLPPFTRPPLMSQAATNTVIVTNSENARSWIKD
                     SQTHQWRLGEPIELRRAMNVIHGDGGGLSGGAAAGVTVGVTTILAVVITLAIYGTRKF
                     KKKAYQAIEERQSLVPGTAATGDTTYQEQGQSPA"
     regulatory      3836..3841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MPEG1"
                     /gene_synonym="IMD77; Mpg-1; MPG1; MPS1; P-2"
                     /note="hexamer: ATTAAA"
     polyA_site      3854
                     /gene="MPEG1"
                     /gene_synonym="IMD77; Mpg-1; MPG1; MPS1; P-2"
                     /note="major polyA site"
     regulatory      4081..4086
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MPEG1"
                     /gene_synonym="IMD77; Mpg-1; MPG1; MPS1; P-2"
                     /note="hexamer: ATTAAA"
     polyA_site      4101
                     /gene="MPEG1"
                     /gene_synonym="IMD77; Mpg-1; MPG1; MPS1; P-2"
     regulatory      4395..4400
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MPEG1"
                     /gene_synonym="IMD77; Mpg-1; MPG1; MPS1; P-2"
                     /note="hexamer: ATTAAA"
     polyA_site      4418
                     /gene="MPEG1"
                     /gene_synonym="IMD77; Mpg-1; MPG1; MPS1; P-2"
ORIGIN      
        1 agtttgccca aagcttgctg gccgctgtgt agggctggtg agtggctggg gctgtctgag
       61 ccatgaacaa cttcagggcc accatcctct tctgggcagc ggcagcatgg gctaaatcag
      121 gcaagccttc gggagagatg gacgaagttg gagttcaaaa atgcaagaat gccttgaaac
      181 tacctgtcct ggaagtccta cctggagggg gctgggacaa tctgcggaat gtggacatgg
      241 gacgagttat ggaattgact tactccaact gcaggacaac agaggatgga cagtatatca
      301 tccctgatga aatcttcacc attccccaga aacagagcaa cctggagatg aactcagaaa
      361 tcctggaatc ctgggcaaat taccagagta gcacctccta ctccatcaac acagaactct
      421 ctcttttttc caaagtcaat ggcaagtttt ccactgagtt ccagaggatg aagaccctcc
      481 aagtgaagga ccaagctata actacccgag ttcaggtaag aaacctcgtc tacacagtca
      541 aaatcaaccc aactttagag ctaagctcag gttttaggaa ggaactcctt gacatctctg
      601 accgtctaga gaacaaccag acgaggatgg ccacctacct ggcagaactc ctggtgctca
      661 actatggcac ccacgtcacc accagtgtcg acgctggggc tgctcttatt caggaggacc
      721 acctcagggc ctccttcctc caagacagcc agagcagtcg tagtgccgtg accgcctctg
      781 ctggacttgc ctttcaaaac accgtgaact tcaaatttga ggaaaactat acctcgcaga
      841 atgtcctcac caagagctac ctctcaaacc gaaccaactc cagggtgcag agcattggag
      901 gggttccttt ttacccaggc atcaccctcc aggcctggca gcagggtatc accaaccacc
      961 tggtggccat cgaccgctct ggcctgccgc tgcatttctt catcaacccc aacatgctac
     1021 ctgacttgcc aggccccctg gtgaagaagg tgtcaaagac agtggaaact gctgtgaagc
     1081 gctattatac attcaacacc taccctggct gcacagatct caattctccc aacttcaatt
     1141 ttcaggccaa cacggatgat ggctcctgcg aggggaaaat gaccaacttc tctttcggtg
     1201 gggtttatca ggaatgcact cagctctcag ggaataggga tgtcctcctc tgccaaaagt
     1261 tggagcagaa gaatccactc actggtgatt tctcctgccc ctctggctac tccccggtgc
     1321 acctgttatc ccagatccac gaggagggtt acaaccacct ggagtgtcat cgaaagtgca
     1381 ctctcctcgt cttctgcaag accgtgtgtg aagatgtgtt ccaggtggca aaagctgaat
     1441 ttagggcttt ttggtgtgtg gccagcagcc aagtacctga aaactcagga ctgctttttg
     1501 ggggcctctt cagcagcaag agcataaacc ccatgacaaa tgcacagtca tgcccagccg
     1561 gctactttcc actgagactc tttgaaaacc tcaaggtatg tgtttctcag gactatgagt
     1621 tgggaagcag gtttgcggtc ccctttggcg ggttctttag ctgcacagtt gggaaccccc
     1681 tggtagatcc tgctatatcc agagatttag gggcaccgtc tctgaaaaag tgccccgggg
     1741 gcttcagcca gcacccagcc ctcatcagcg atggatgcca agtgtcctat tgcgtcaaat
     1801 ccgggctctt cacaggaggg tccctgcccc ctgccaggct cccacctttc acccggccac
     1861 ccctcatgag tcaggctgcc accaatactg tcatagtgac caattctgag aatgcgagat
     1921 cctggattaa agactcccag acccaccagt ggaggctggg agaaccgata gagctgcgga
     1981 gggccatgaa tgtcatccat ggggatggtg gtggtctgtc aggaggggct gcagctgggg
     2041 tcacagtggg ggtcaccacc attctggctg ttgttatcac cttggccatc tacggcaccc
     2101 ggaagttcaa gaagaaagca tatcaggcaa ttgaggaaag gcagagtttg gttccaggca
     2161 ctgcagcaac tggagacacc acttaccaag agcaggggca gagtccagct taaatctctc
     2221 cccgaaaatg gtttctctca tctccagtgt ggtcattgct gaccactctg ttttcctaag
     2281 cattgaaatg gcaagtgcaa ccaaaagtag gtatattcgt gacttcttgt ttaggtctct
     2341 gggccaggaa attcatactg ttacatggat aaggttggga ttggggagag ggaacagttg
     2401 ggactagaag caaaagtgat tctgggacta aaataggaag cagatgtcct ttcccaatgt
     2461 gtgttgctgt cttcacctga atgcatttgt gtaaaaatag cggagggaca atgtgaacat
     2521 ttgtatttgg aagctatgaa tttactctga agtttgcagt tgtttccaat ttgtgagctc
     2581 taagagtttc tgcctgtaag aactactctc cttttatttt gatttttaaa aacctgtctg
     2641 aatttcacac tcttagagcc tggaagagcc ctgaaaagac acaagtcttg cctggctact
     2701 gctttttaac tttgagggct ctatgttgac agactgttat ctcctctggg tgacctcaaa
     2761 catctgaaaa gaaagatgtt gcctgtgcca attccacttt ttccagctgc cccttgatga
     2821 acactccctt ataccagacc actcttggac ttctgactgg tgtcatcaag tcctcagaaa
     2881 atattttaag ttattttaag ttattaagga agggatgatt tggagacaag gagtaatgaa
     2941 agatgggtaa aaactggaaa agattctggt gctaagtact accccttcat cttccatgga
     3001 tggtcattac ctttcctgtc ctcctgttat atgaacacac acacacacac acacacacac
     3061 acacacacac acgcacacac ataccacatt tcaataagtc ttcattgttc tgggtcctta
     3121 cctttcctgt cctcctgtta tatgaacaca cacacacaca cacacacaca cacacacgca
     3181 caccacattt caatgtctgc atggttctgg gggtaacaaa ggcaagaagt taaagtaaga
     3241 ccacatttga gtattactta ctctgtagaa tcaaaacaga gtagttaaca ccaattatgc
     3301 aaactacttt tttttccagc agaaagggag ctgacatgat caaatccatg ttttcaaatg
     3361 aactgaaaaa ggcatccagc accacttata acacatttat ttcagttcca ggctgacagc
     3421 cctggggcat ctagcaggat gcataattgt catctggtga gagttgggac tttgctcaat
     3481 tttaatacca atctctcctg attgtaatta cctccactac tttcatgatc cccctacaat
     3541 atttttttaa atgatatatt tattcactga atcaaatgtc attaatgagt tatcttctgt
     3601 ggaggatgac tgttctcttt gttaatgttc acaatcaaga tcttgggctg agaagaggcc
     3661 cttcacccaa ggagtttgaa gtatcacagt gtgtgggaag gtgggaacca ggatacccat
     3721 tcatttccaa ccgagacaca gagaagtgag tcacagaatt tgagcccgct ctcttgactg
     3781 cccagccaga gacactgatt tctgtaacct cttcacttga tcctgcctct taagcattaa
     3841 aacattctcc taaacctctg agtgttttgt gggttctggg tgttttgtac attttagcca
     3901 agctaaccac ttgtctgcaa gtactgactt tcctatgaat tctttgaaga ttattgagtc
     3961 agaaaggaaa aatatagccc caaattccca ggcttttaat gcattacatt aactgcctat
     4021 tgaaatgaga agttcttcac aaacttgtat acccactaac aagattgcac ataaacatgc
     4081 attaaagtat atactaagaa accctctgtc caacggctca tgcatatgaa gtccgaacat
     4141 gggagtttgc caattgcatt catcaagtcg ttttgcggag tcagatccct gatggaagag
     4201 ctcacaggct ctgccttcca agtcctgggt tcctaactgg tgaccttagc ctggggtctg
     4261 tggggagacc aaccctggct tccaagaaaa ccacattcca tggactatca gaaatagaca
     4321 cagatttggg tgacaaagct ggctctgtat ttgcatttta tttttgtgtt cttgtcagtt
     4381 tgggaatgat taatattaaa catttatttg agaaacaa
//
