LOCUS       NM_002431               2648 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens MNAT1 component of CDK activating kinase (MNAT1),
            transcript variant 1, mRNA.
ACCESSION   NM_002431
VERSION     NM_002431.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Li,H., Dai,H., Shi,T., Cheng,X., Sun,M., Chen,K., Wang,M. and
            Wei,Q.
  TITLE     Potentially functional variants in nucleotide excision repair
            pathway genes predict platinum treatment response of Chinese
            ovarian cancer patients
  JOURNAL   Carcinogenesis 41 (9), 1229-1237 (2020)
   PUBMED   32663249
  REMARK    GeneRIF: Potentially functional variants in nucleotide excision
            repair pathway genes predict platinum treatment response of Chinese
            ovarian cancer patients.
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 2648)
  AUTHORS   Wang,J., Ni,J., Song,D., Ding,M., Huang,J., Li,W. and He,G.
  TITLE     The regulatory effect of has-circ-0001146/miR-26a-5p/MNAT1 network
            on the proliferation and invasion of osteosarcoma
  JOURNAL   Biosci Rep 40 (6) (2020)
   PUBMED   32453410
  REMARK    GeneRIF: The regulatory effect of has-circ-0001146/miR-26a-5p/MNAT1
            network on the proliferation and invasion of osteosarcoma.
REFERENCE   4  (bases 1 to 2648)
  AUTHORS   Peissert,S., Sauer,F., Grabarczyk,D.B., Braun,C., Sander,G.,
            Poterszman,A., Egly,J.M., Kuper,J. and Kisker,C.
  TITLE     In TFIIH the Arch domain of XPD is mechanistically essential for
            transcription and DNA repair
  JOURNAL   Nat Commun 11 (1), 1667 (2020)
   PUBMED   32245994
  REMARK    GeneRIF: Study presents the high-resolution crystal structure of
            the Arch domain of XPD with its interaction partner MAT1, a central
            component of the CDK activating kinase complex. The analysis of the
            interface led to the identification of amino acid residues that are
            crucial for the MAT1-XPD interaction.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2648)
  AUTHORS   Wang,J., Ni,J., Song,D., Ding,M., Huang,J., Li,W. and He,G.
  TITLE     MAT1 facilitates the lung metastasis of osteosarcoma through
            upregulation of AKT1 expression
  JOURNAL   Life Sci 234, 116771 (2019)
   PUBMED   31421084
  REMARK    GeneRIF: MAT1 serves as a promoter in the lung metastasis of
            osteosarcoma through increasing AKT1 expression.
REFERENCE   6  (bases 1 to 2648)
  AUTHORS   Aprelikova,O., Xiong,Y. and Liu,E.T.
  TITLE     Both p16 and p21 families of cyclin-dependent kinase (CDK)
            inhibitors block the phosphorylation of cyclin-dependent kinases by
            the CDK-activating kinase
  JOURNAL   J Biol Chem 270 (31), 18195-18197 (1995)
   PUBMED   7629134
REFERENCE   7  (bases 1 to 2648)
  AUTHORS   Desai,D., Wessling,H.C., Fisher,R.P. and Morgan,D.O.
  TITLE     Effects of phosphorylation by CAK on cyclin binding by CDC2 and
            CDK2
  JOURNAL   Mol Cell Biol 15 (1), 345-350 (1995)
   PUBMED   7799941
REFERENCE   8  (bases 1 to 2648)
  AUTHORS   Le Goff,P., Montano,M.M., Schodin,D.J. and Katzenellenbogen,B.S.
  TITLE     Phosphorylation of the human estrogen receptor. Identification of
            hormone-regulated sites and examination of their influence on
            transcriptional activity
  JOURNAL   J Biol Chem 269 (6), 4458-4466 (1994)
   PUBMED   8308015
REFERENCE   9  (bases 1 to 2648)
  AUTHORS   Jacob,G.A., Luse,S.W. and Luse,D.S.
  TITLE     Abortive initiation is increased only for the weakest members of a
            set of down mutants of the adenovirus 2 major late promoter
  JOURNAL   J Biol Chem 266 (33), 22537-22544 (1991)
   PUBMED   1939271
REFERENCE   10 (bases 1 to 2648)
  AUTHORS   Conaway,R.C. and Conaway,J.W.
  TITLE     ATP activates transcription initiation from promoters by RNA
            polymerase II in a reversible step prior to RNA synthesis
  JOURNAL   J Biol Chem 263 (6), 2962-2968 (1988)
   PUBMED   2449431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CD701743.1, BC000820.1 and
            AL160236.4.
            
            On Nov 22, 2018 this sequence version replaced NM_002431.3.
            
            Summary: The protein encoded by this gene, along with cyclin H and
            CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This
            complex activates several cyclin-associated kinases and can also
            associate with TFIIH to activate transcription by RNA polymerase
            II. Two transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Sep 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.312590.1,
                                           SRR1660803.166695.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261245.9/ ENSP00000261245.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                CD701743.1         38-53
            17-1363             BC000820.1         16-1362
            1364-2648           AL160236.4         48977-50261         c
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.1"
     gene            1..2648
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /note="MNAT1 component of CDK activating kinase"
                     /db_xref="GeneID:4331"
                     /db_xref="HGNC:HGNC:7181"
                     /db_xref="MIM:602659"
     exon            1..191
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..1032
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     menage a trois 1 (CAK assembly factor); cyclin G1
                     interacting protein; CDK-activating kinase assembly factor
                     MAT1; MNAT1, CDK activating kinase assembly factor; menage
                     a trois homolog 1, cyclin H assembly factor; RING finger
                     protein 66; RING finger protein MAT1; CDK7/cyclin-H
                     assembly factor; menage a trois-like protein 1 cyclin H
                     assembly factor; MNAT CDK-activating kinase assembly
                     factor 1"
                     /codon_start=1
                     /product="CDK-activating kinase assembly factor MAT1
                     isoform 1"
                     /protein_id="NP_002422.1"
                     /db_xref="CCDS:CCDS9750.1"
                     /db_xref="GeneID:4331"
                     /db_xref="HGNC:HGNC:7181"
                     /db_xref="MIM:602659"
                     /translation="MDDQGCPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFVRGAG
                     NCPECGTPLRKSNFRVQLFEDPTVDKEVEIRKKVLKIYNKREEDFPSLREYNDFLEEV
                     EEIVFNLTNNVDLDNTKKKMEIYQKENKDVIQKNKLKLTREQEELEEALEVERQENEQ
                     RRLFIQKEEQLQQILKRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEMQLEKPKPV
                     KPVTFSTGIKMGQHISLAPIHKLEEALYEYQPLQIETYGPHVPELEMLGRLGYLNHVR
                     AASPQDLAGGYTSSLACHRALQDAFSGLFWQPS"
     exon            192..344
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /inference="alignment:Splign:2.1.0"
     exon            345..418
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /inference="alignment:Splign:2.1.0"
     exon            419..522
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /inference="alignment:Splign:2.1.0"
     exon            523..663
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /inference="alignment:Splign:2.1.0"
     exon            664..789
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /inference="alignment:Splign:2.1.0"
     exon            790..911
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /inference="alignment:Splign:2.1.0"
     exon            912..2648
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1341..1346
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /note="hexamer: AATAAA"
     polyA_site      1363
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /note="major polyA site"
     regulatory      2540..2545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /note="hexamer: AATAAA"
     polyA_site      2564
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
     regulatory      2617..2622
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
                     /note="hexamer: AATAAA"
     polyA_site      2648
                     /gene="MNAT1"
                     /gene_synonym="CAP35; MAT1; RNF66; TFB3"
ORIGIN      
        1 aagcgcctgt tggtaggaac ctgcttggtc gcgtctgagg gggcttgtag gtggctctgg
       61 ctgaaacagg cgcctgcgag agtctgtagg agggaaaccg ccatggacga tcagggttgc
      121 cctcggtgta agaccaccaa atatcggaac ccctccttga agctgatggt gaatgtgtgc
      181 ggacacactc tctgtgaaag ttgtgtagat ttactgtttg tgagaggagc tggaaactgc
      241 cctgagtgtg gtactccact cagaaagagc aacttcaggg tacaactctt tgaagatccc
      301 actgttgaca aggaggttga gatcaggaaa aaagtgctaa agatatacaa taaaagggaa
      361 gaagattttc ctagtctaag agaatacaat gatttcttgg aagaagtgga agaaattgtt
      421 ttcaacttga ccaacaatgt ggatttggac aacaccaaaa agaaaatgga gatataccaa
      481 aaggaaaaca aagatgttat tcagaaaaat aaattaaagc tgactcgaga acaggaagaa
      541 ctggaagaag ctttagaagt ggaacgacag gaaaatgaac aaagaagatt atttatacaa
      601 aaagaagaac aactgcagca gattctaaaa aggaagaata agcaggcttt tttagatgag
      661 ctggagagtt ctgatctccc tgttgctctg cttttggctc agcataaaga tagatctacc
      721 caattagaaa tgcaacttga gaaacccaaa cctgtaaaac cagtgacgtt ttccacaggc
      781 atcaaaatgg gtcaacatat ttcactggca cctattcaca agcttgaaga agctctgtat
      841 gaataccagc cactgcagat agagacatat ggaccacatg ttcctgagct tgagatgcta
      901 ggaagacttg ggtatttaaa ccatgtcaga gctgcctcac cacaggacct tgctggaggc
      961 tatacttctt ctcttgcttg tcacagagca ctacaggatg cattcagtgg gcttttctgg
     1021 cagcccagtt aaccatttat aagatttgga ccttggagct gaaccaggga gctagcaaaa
     1081 gtaaagcaga cttataaaat tatagctatg tgcagctgca caacacagtc cttccactag
     1141 cagctgtgtt aaagtattta taaggagaaa atttcagaac taagttgagt aatatagggg
     1201 atatatattt gtgaaaaata atttttactt atatttttca gaggatttga cacgataagc
     1261 ctcatctgat ggaagagagg aataaataat tcacctatat gtgtttgagg ttgtgacaga
     1321 cttataaaat ctttttaaaa aataaagcta taatttatat taagttctgt ggtttttctc
     1381 ttattacagt gttttacttg aacacatttt aaataccatt ttgattacaa catttactgt
     1441 taatatccaa gtctattatt tcttctcata aaatgttccc ctttttccta atgtcttgtt
     1501 aatactagtc cagacaagtg gatatatttc ctttgaccaa gttatggcaa gaaaaacctg
     1561 acccatgata atgtcattcc ctcaatacta aggactcaat gcctgtttta agcagtggaa
     1621 aatgaggcaa accttgatca aggtgttata gttcccagct gtgagccaca ctcaggataa
     1681 aacaaaaccc agcattaacc tgctttagtg acacattcaa agaagttaat aaaatttaat
     1741 acttaaatga tacctttcac ctccaatgta agctcatttt atgatgaatc ctttggagac
     1801 tggagaaggt acattttaag tacagttccc tatgtataaa ttgtatactg atacagtttt
     1861 tttgttgaaa cttcaaaggc ttttattacc agggttttgc ttaaagagtt tagatatgaa
     1921 taggatgata ttgcagcatc tcaaccttgt atgagtctgt tacatagagc attggatatg
     1981 ggatatagtt tcttaaggaa attaagggat ttcctatcaa atctctatta caacagatgt
     2041 tagttttatg ggtagaaact taggtgaaac aagacttgtg gttacattag gatggactat
     2101 ccatctgtaa caactccatc ttattttact aagcagttat taattaattt tagcaataca
     2161 tcttcatctt tattgtactt ctatctttgt taaatgagaa aaccaaggca tcaaataatt
     2221 gagtagacaa agatcatatt cacaagttag taaaaagcca gaattagaat gcaggttttt
     2281 tttttcaaag tccagttcca tctacattct accagactgc atatcctatt cctgtatcta
     2341 ttaggtattc ccgaactgag gattagtgtt tttaagtaca gtctataaat ctgttcaaag
     2401 aatagttaga acattgaatg tagagttaag gctttgcagc agccaattgc aaaaataagg
     2461 aatccagtct tttgaaaaga aacactactg cttgttctca taccttttat aaatgtattt
     2521 taaaaagaat gaactaatta ataaaacata tcaaaggctt ttaaggcttg ttttgagcct
     2581 gccttattgt ggaaattgca tgcacctggt ttggcaaata aattccaaaa cctttataca
     2641 tttgtaaa
//
