LOCUS       NM_198468               8683 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens MMS22 like, DNA repair protein (MMS22L), transcript
            variant 1, mRNA.
ACCESSION   NM_198468 XM_171118
VERSION     NM_198468.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   van Schie,J.J., de Lint,K., Pai,G.M., Rooimans,M.A., Wolthuis,R.M.
            and de Lange,J.
  TITLE     MMS22L-TONSL functions in sister chromatid cohesion in a pathway
            parallel to DSCC1-RFC
  JOURNAL   Life Sci Alliance 6 (2), e202201596 (2022)
   PUBMED   36622344
  REMARK    GeneRIF: MMS22L-TONSL functions in sister chromatid cohesion in a
            pathway parallel to DSCC1-RFC.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   Huang,T.H., Fowler,F., Chen,C.C., Shen,Z.J., Sleckman,B. and
            Tyler,J.K.
  TITLE     The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated
            Rad51 Loading onto ssDNA during Homologous Recombination in Human
            Cells
  JOURNAL   Mol Cell 69 (5), 879-892 (2018)
   PUBMED   29478807
  REMARK    GeneRIF: uncovered DNA-PKcs-dependent DNA damage-induced ASF1A
            phosphorylation, which enhances chromatin assembly, promoting
            MMS22L-TONSL recruitment and, hence, Rad51 loading.
            Erratum:[Mol Cell. 2020 Mar 5;77(5):1153. doi:
            10.1016/j.molcel.2020.02.019.. PMID: 32142686]
REFERENCE   3  (bases 1 to 8683)
  AUTHORS   Schrors,B., Lubcke,S., Lennerz,V., Fatho,M., Bicker,A., Wolfel,C.,
            Derigs,P., Hankeln,T., Schadendorf,D., Paschen,A. and Wolfel,T.
  TITLE     HLA class I loss in metachronous metastases prevents continuous T
            cell recognition of mutated neoantigens in a human melanoma model
  JOURNAL   Oncotarget 8 (17), 28312-28327 (2017)
   PUBMED   28423700
REFERENCE   4  (bases 1 to 8683)
  AUTHORS   Piwko,W., Mlejnkova,L.J., Mutreja,K., Ranjha,L., Stafa,D.,
            Smirnov,A., Brodersen,M.M., Zellweger,R., Sturzenegger,A.,
            Janscak,P., Lopes,M., Peter,M. and Cejka,P.
  TITLE     The MMS22L-TONSL heterodimer directly promotes RAD51-dependent
            recombination upon replication stress
  JOURNAL   EMBO J 35 (23), 2584-2601 (2016)
   PUBMED   27797818
  REMARK    GeneRIF: By specifically regulating RAD51 activity at uncoupled
            replication forks, MMS22L-TONSL stabilizes perturbed replication
            forks by promoting replication fork reversal and stimulating their
            homologous recombination-mediated restart in vivo.
REFERENCE   5  (bases 1 to 8683)
  AUTHORS   Saredi,G., Huang,H., Hammond,C.M., Alabert,C., Bekker-Jensen,S.,
            Forne,I., Reveron-Gomez,N., Foster,B.M., Mlejnkova,L., Bartke,T.,
            Cejka,P., Mailand,N., Imhof,A., Patel,D.J. and Groth,A.
  TITLE     H4K20me0 marks post-replicative chromatin and recruits the
            TONSL-MMS22L DNA repair complex
  JOURNAL   Nature 534 (7609), 714-718 (2016)
   PUBMED   27338793
  REMARK    GeneRIF: new histones incorporated during DNA replication provide a
            signature of post-replicative chromatin, read by the human
            TONSL-MMS22L homologous recombination complex
REFERENCE   6  (bases 1 to 8683)
  AUTHORS   Piwko,W., Buser,R. and Peter,M.
  TITLE     Rescuing stalled replication forks: MMS22L-TONSL, a novel complex
            for DNA replication fork repair in human cells
  JOURNAL   Cell Cycle 10 (11), 1703-1705 (2011)
   PUBMED   21519189
REFERENCE   7  (bases 1 to 8683)
  AUTHORS   Piwko,W., Olma,M.H., Held,M., Bianco,J.N., Pedrioli,P.G.,
            Hofmann,K., Pasero,P., Gerlich,D.W. and Peter,M.
  TITLE     RNAi-based screening identifies the Mms22L-Nfkbil2 complex as a
            novel regulator of DNA replication in human cells
  JOURNAL   EMBO J 29 (24), 4210-4222 (2010)
   PUBMED   21113133
  REMARK    GeneRIF: Results strongly suggest that the Mms22L-Nfkbil2 complex
            contributes to genome stability by regulating the chromatin state
            at stalled replication forks.
REFERENCE   8  (bases 1 to 8683)
  AUTHORS   O'Connell,B.C., Adamson,B., Lydeard,J.R., Sowa,M.E., Ciccia,A.,
            Bredemeyer,A.L., Schlabach,M., Gygi,S.P., Elledge,S.J. and
            Harper,J.W.
  TITLE     A genome-wide camptothecin sensitivity screen identifies a
            mammalian MMS22L-NFKBIL2 complex required for genomic stability
  JOURNAL   Mol Cell 40 (4), 645-657 (2010)
   PUBMED   21055985
  REMARK    GeneRIF: This study identifies MMS22L-NFKBIL2 as components of the
            replication stress control pathway.
REFERENCE   9  (bases 1 to 8683)
  AUTHORS   Duro,E., Lundin,C., Ask,K., Sanchez-Pulido,L., MacArtney,T.J.,
            Toth,R., Ponting,C.P., Groth,A., Helleday,T. and Rouse,J.
  TITLE     Identification of the MMS22L-TONSL complex that promotes homologous
            recombination
  JOURNAL   Mol Cell 40 (4), 632-644 (2010)
   PUBMED   21055984
  REMARK    GeneRIF: MMS22L and TONSL are required for the maintenance of
            genome stability.
REFERENCE   10 (bases 1 to 8683)
  AUTHORS   O'Donnell,L., Panier,S., Wildenhain,J., Tkach,J.M., Al-Hakim,A.,
            Landry,M.C., Escribano-Diaz,C., Szilard,R.K., Young,J.T., Munro,M.,
            Canny,M.D., Kolas,N.K., Zhang,W., Harding,S.M., Ylanko,J.,
            Mendez,M., Mullin,M., Sun,T., Habermann,B., Datti,A., Bristow,R.G.,
            Gingras,A.C., Tyers,M.D., Brown,G.W. and Durocher,D.
  TITLE     The MMS22L-TONSL complex mediates recovery from replication stress
            and homologous recombination
  JOURNAL   Mol Cell 40 (4), 619-631 (2010)
   PUBMED   21055983
  REMARK    GeneRIF: These results indicate that MMS22L and TONSL are genome
            caretakers that stimulate the recombination-dependent repair of
            stalled or collapsed replication forks.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY007888.1, AK128060.1,
            AL590484.10 and AL023656.8.
            
            On Jun 2, 2019 this sequence version replaced NM_198468.3.
            
            Summary: The protein encoded by this gene forms a complex with
            tonsoku-like, DNA repair protein (TONSL), and this complex
            recognizes and repairs DNA double-strand breaks at sites of stalled
            or collapsed replication forks. The encoded protein also can bind
            with the histone-associated protein NFKBIL2 to help regulate the
            chromatin state at stalled replication forks. Finally, this gene
            appears to be overexpressed in most lung and esophageal cancers.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Apr 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a). Variants 1 and 2
            both encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK128060.1, SRR14038196.390373.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                HY007888.1         63-102
            41-1179             AK128060.1         1-1139
            1180-1180           AL590484.10        58932-58932         c
            1181-1991           AK128060.1         1141-1951
            1992-1997           AL590484.10        24771-24776         c
            1998-2929           AK128060.1         1958-2889
            2930-2930           AL023656.8         86288-86288         c
            2931-3485           AK128060.1         2891-3445
            3486-3486           AL023656.8         73077-73077         c
            3487-4309           AK128060.1         3447-4269
            4310-8683           AL023656.8         49951-54324         c
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q16.1"
     gene            1..8683
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="MMS22 like, DNA repair protein"
                     /db_xref="GeneID:253714"
                     /db_xref="HGNC:HGNC:21475"
                     /db_xref="MIM:615614"
     exon            1..230
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            231..470
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     CDS             307..4038
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="isoform a is encoded by transcript variant 1"
                     /codon_start=1
                     /product="protein MMS22-like isoform a"
                     /protein_id="NP_940870.2"
                     /db_xref="CCDS:CCDS5039.1"
                     /db_xref="GeneID:253714"
                     /db_xref="HGNC:HGNC:21475"
                     /db_xref="MIM:615614"
                     /translation="MENCSAASTFLTDSLELELGTEWCKPPYFSCAVDNRGGGKHFSG
                     ESYLCSGALKRLILNLDPLPTNFEEDTLEIFGIQWVTETALVNSSRELFHLFRQQLYN
                     LETLLQSSCDFGKVSTLHCKADNIRQQCVLFLHYVKVFIFRYLKVQNAESHVPVHPYE
                     ALEAQLPSVLIDELHGLLLYIGHLSELPSVNIGAFVNQNQIKLFPPSWHLLHLHLDIH
                     WLVLEILYMLGEKLKQVVYGHQFMNLASDNLTNISLFEEHCETLLCDLISLSLNRYDK
                     VRSSESLMSDQCPCLCIKELWVLLIHLLDHRSKWFVSESFWNWLNKLLKTLLEKSSDR
                     RRSSMPVIQSRDPLGFSWWIITHVASFYKFDRHGVPDEMRKVESNWNFVEELLKKSIS
                     VQGVILEEQLRMYLHCCLTLCDFWEPNIAIVTILWEYYSKNLNSSFSISWLPFKGLAN
                     TMKSPLSMLEMVKTCCCDKQDQELYKSSSSYTIFLCILAKVVKKAMKSNGPHPWKQVK
                     GRIYSKFHQKRMEELTEVGLQNFFSLFLLLAAVAEVEDVASHVLDLLNFLKPAFVTSQ
                     RALIWKGHMAFLLMYAQKNLDIGVLAEKFSCAFREKAKEFLVSKNEEMVQRQTIWTLL
                     SIYIDGVQEVFETSYCLYPSHEKLLNDGFSMLLRACRESELRTVLSFLQAVLARIRSM
                     HQQLCQELQRDNVDLFVQSSLSAKERHLAAVASALWRHFFSFLKSQRMSQVVPFSQLA
                     DAAADFTLLAMDMPSTAPSDFQPQPVISIIQLFGWDDIICPQVVARYLSHVLQNSTLC
                     EALSHSGYVSFQALTVRSWIRCVLQMYIKNLSGPDDLLIDKNLEEAVEKEYMKQLVKL
                     TRLLFNLSEVKSIFSKAQVEYLSISEDPKKALVRFFEAVGVTYGNVQTLSDKSAMVTK
                     SLEYLGEVLKYIKPYLGKKVFSAGLQLTYGMMGILVKSWAQIFATSKAQKLLFRIIDC
                     LLLPHAVLQQEKELPAPMLSAIQKSLPLYLQGMCIVCCQSQNPNAYLNQLLGNVIEQY
                     IGRFLPASPYVSDLGQHPVLLALRNTATIPPISSLKKCIVQVIRKSYLEYKGSSPPPR
                     LASILAFILQLFKETNTDIYEVELLLPGILKCLVLVSEPQVKRLATENLQYMVKACQV
                     GSEEEPSSQLTSVFRQFIQDYGMRYYYQVYSILETVATLDQQVVIHLISTLTQSLKDS
                     EQKWGLGRNIAQREAYSKLLSHLGQMGQDEMQRLENDNT"
     exon            471..596
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            597..646
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            647..734
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            735..912
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1003
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1134
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1248
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1425
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1488
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1608
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1835
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..2345
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2539
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2690
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..2842
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..2985
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2986..3145
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3146..3315
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3316..3527
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3528..3691
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3692..3788
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3789..3956
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3957..8683
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4289..4294
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="hexamer: ATTAAA"
     polyA_site      4310
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="major polyA site"
     regulatory      8661..8666
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="hexamer: AATAAA"
     polyA_site      8683
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="major polyA site"
ORIGIN      
        1 ctttccggag cgcgggcgcg cggtggcggg aatttcgcct gtttgcggtt tagaccccaa
       61 agattcctgt tggtggtctg ggtcacagga ggcaggtttc gggagctgga aatgtgagcg
      121 ggtacgacag gcaccgcggg taaccgacgc cccgggtcct tgctgcagcc gggtacgcgg
      181 gataccggca ccccgccttc tccgcccgag tgctgccagg cgtgggcctg gaatctcttc
      241 acaccttctc tttggagccc ttaatgatac gacgaacccc aagtgtttca gaacatgaag
      301 taaacaatgg agaactgttc tgctgcatcg acgttcctga ctgacagctt agagctggag
      361 ctggggacgg aatggtgcaa acctccttac ttttcttgtg ctgttgacaa cagaggagga
      421 ggaaaacatt tttctggaga atcctacctc tgcagcggag cccttaagcg attgattttg
      481 aatcttgacc ctttaccaac taattttgaa gaagatacct tggaaatatt tggcattcag
      541 tgggttactg aaacagcatt agtgaattca tctagagaac tctttcattt attcaggcaa
      601 caactgtaca acttggaaac cttgttacag tccagttgtg attttgggaa ggtatcaact
      661 ctacactgca aagcagacaa tattaggcag cagtgtgtac tatttctcca ttatgttaaa
      721 gttttcatct tcaggtatct gaaagtacag aatgctgaga gtcatgttcc tgtccatcct
      781 tatgaggctt tggaggctca gcttccctca gtgttgattg atgagcttca tggattactc
      841 ttgtatattg gacacctatc tgaacttccc agtgttaata taggagcatt tgtaaatcaa
      901 aaccagatta agctttttcc accgtcatgg catttattac atctccactt ggatatacat
      961 tggctggtgc tagaaattct ttacatgctg ggtgaaaaat tgaaacaagt tgtatatggt
     1021 catcagttta tgaatctggc aagtgacaat ttaaccaaca tcagcctatt tgaagaacat
     1081 tgtgaaactc tcctttgtga tttaataagc ctgtcactca acaggtacga caaggttagg
     1141 tcttctgaat cattaatgag tgaccagtgt ccatgtttat gcattaaaga attatgggtt
     1201 ctacttattc atcttctaga ccacagaagt aaatggtttg tctcggaatc attttggaac
     1261 tggttgaata aactacttaa aacactgctt gaaaaatcaa gtgaccgaag aagatcctct
     1321 atgcctgtaa tccagtccag ggatccatta ggttttagtt ggtggattat tactcatgta
     1381 gcatcatttt acaagtttga tcgccatgga gtaccagatg aaatgagaaa agtggaatca
     1441 aattggaact ttgtagaaga actgctgaaa aagtccatca gtgttcaggg tgtcattcta
     1501 gaagaacaat tacgaatgta tcttcactgt tgtttgacac tttgtgattt ctgggagcca
     1561 aacattgcaa ttgttaccat tttatgggaa tattatagta agaacctgaa tagttccttc
     1621 agtatttctt ggcttccttt taaaggcctt gctaatacca tgaagtcacc cttgtctatg
     1681 cttgaaatgg tgaagacttg ctgttgcgat aaacaagatc aggaactata taaatccagc
     1741 agtagttata ctatttttct ttgtattctg gcaaaagttg ttaaaaaagc aatgaagagc
     1801 aatggccctc atccttggaa acaagtcaaa ggaagaatat attcaaaatt ccatcaaaaa
     1861 agaatggaag aactaactga agttggtcta cagaactttt ttagcctttt tctactgtta
     1921 gcagctgttg cagaggtaga agatgttgca agtcatgttt tagacctcct gaatttcctc
     1981 aagcctgctt ttgtaacgtc tcagagagcc ctcatttgga agggtcacat ggccttcctc
     2041 ttgatgtatg cccagaaaaa tctggacatt ggtgttttgg ctgagaaatt ttcatgtgct
     2101 ttccgggaga aagcaaagga attcttggtg tctaagaatg aggaaatggt acagagacag
     2161 actatctgga cccttctttc catatacatt gatggtgttc aagaagtgtt tgagaccagc
     2221 tattgcttgt atccttccca tgaaaaactg cttaatgatg gatttagtat gcttctgcga
     2281 gcatgtcgag aatctgaact taggacagta ttgagcttcc tacaagctgt tctggccaga
     2341 atcaggagta tgcatcaaca attgtgtcag gaacttcaaa gggacaatgt ggacctattt
     2401 gtacagtctt cattatcggc taaagagcgc caccttgctg cagttgccag tgcactgtgg
     2461 agacatttct tttcattttt gaagagtcag agaatgtcac aggtagtgcc tttctcacaa
     2521 cttgcggatg cagctgcaga ctttactttg ctagcaatgg acatgccaag cacagctcca
     2581 tcagattttc agcctcagcc agttatatca attattcaac tttttggttg ggatgatatc
     2641 atctgccctc aagttgtagc aagatattta agtcatgtcc tacaaaatag cacattatgt
     2701 gaagcacttt ctcattcagg ctatgtatct tttcaagcct taaccgtaag atcatggatt
     2761 cgttgtgttt tgcaaatgta tattaaaaac ctctctgggc ctgatgattt gctcatagat
     2821 aaaaatctgg aagaggcagt tgaaaaagag tacatgaaac agttggtcaa actgacaaga
     2881 ttactattta atctctcaga agtaaagagt attttctcaa aggcccaagt tgaatattta
     2941 tccatctcag aagaccctaa aaaagcactt gttcgattct ttgaggctgt tggtgtaact
     3001 tacgggaacg tccagacact ttctgataaa tctgccatgg tcacaaagtc cttggaatac
     3061 cttggtgaag tattaaaata tattaagcct tatttgggaa aaaaagtttt cagtgcaggg
     3121 ctgcagctga cttatggaat gatgggaatt cttgtgaaat catgggcaca aatctttgcc
     3181 acttctaaag cccaaaaatt actattccgg atcatagatt gtttactgct gccacatgca
     3241 gtattacagc aagagaagga actgcctgca cctatgttgt cagcaattca gaaaagtctt
     3301 cctttgtatc tccagggcat gtgtatcgtg tgttgtcaat ctcaaaatcc gaatgcctat
     3361 ttgaatcaat tgctagggaa tgttattgag cagtatattg ggcgatttct tccagcttca
     3421 ccatatgttt cagatcttgg acaacatcct gttttgctgg cattgagaaa cacagccact
     3481 attccaccaa tatcatctct aaagaaatgc attgtgcaag tcataaggaa atcctacctt
     3541 gagtataagg ggtcctcacc tcctcctcgc ttagcatcca ttctggcctt catcctccaa
     3601 ctcttcaagg aaactaacac agacatttat gaagttgaac tactcctccc tggcatttta
     3661 aaatgcttgg tgttagtcag tgaaccacaa gttaaaaggc tggccacaga gaacctgcaa
     3721 tacatggtaa aagcctgcca agtggggtca gaagaagaac cttcctccca gctgacttct
     3781 gtgtttaggc agtttatcca ggattatggt atgaggtact attaccaggt ttacagcatt
     3841 ttagaaacag tagcaacatt ggaccagcag gttgtcatcc acttgatttc tacccttact
     3901 cagtctctga aggattcaga gcagaaatgg ggccttggca ggaatatagc acaaagggaa
     3961 gcctatagca aacttttgtc tcaccttgga cagatgggac aagatgagat gcagagactg
     4021 gaaaatgata atacttaata tattttgtga ttcatcctgt ctattaatta tcagtatcta
     4081 aagccacatt gttccactct aattcctact tttaattaat tgtataatgt tctgtttaaa
     4141 ataatttcta ggattttttt tccttttata tctgtaagta aaaaatgtat aaataaggaa
     4201 ctgaaggtta attagcaact gtgtaatagg atccttggaa ctttttacaa aactgcattc
     4261 taccttgatg aaattttagt taaactaaat taaagaaaca ttttctgaga gtgttttctg
     4321 tgtgtgagta ttaagcagat acttcatcca tagacttttt gttgtagtat gacttcctgc
     4381 ctccctgcct tgtgactgat ttgtcatgtt tattttttat ttttacaata atagttatgt
     4441 tttaaaatga tactttttca aagtacctgg ctaccctata ctgtgttttt ggtgggggaa
     4501 aggtgttggg aaatgtgact gtgtatttgt ttttcttttt tatatgtgtg aggcaagtta
     4561 acaaagaaaa cccttacaaa gcacacttaa ctgtgttagg aatgagactg aatctggcta
     4621 aaggattata cagctgatat ccaaaaagaa gtgtaaacat gccaacaggg tttatattta
     4681 ggttccaaga gttgccaaat ttttttttta ttcatccttt cttttggagt tgtcattaaa
     4741 aaaaaaatca tatcaggagg aggaaataaa ggagacttta agaggagagt aaataaaaat
     4801 attctagaaa agtaacaata gtagtggtct gagaagtcag ctgatccatc ttcttgctct
     4861 ttagccagag cttcttataa cttagcattt tatccaagaa acctgtttag tttcctttaa
     4921 caacattgtt tctcaacttt tgaactctta actcatccag attgttttac cagttggagc
     4981 ttgtccttga ctccttttcc tattgagcat tatttggttg aaagagctcc agtcctagtt
     5041 ttgggaaccc acctgggtct caaactcagc tttgctttat gatcttgagc tagttacgta
     5101 acctttctca gtctagtttc ttcatggaga agaaaagaag aatcccttta ctatttgaca
     5161 gtgttgttgt gagaatcaat gggataatgt acgtgaaaat atttggtggc ccaaattctg
     5221 ttgggaaatg tacagacacg attagtttat tactcgtgca caattaaggc aaagaacacc
     5281 tctcaatttt atctttctct aatgatatgt cccagatgaa tgatcccact ttccaatgtt
     5341 ttcttacatc atgttttcta actttgaagt actttgtttc cttcttggga ttatagtctt
     5401 tatcatggtt ctactcttcc ttcaagaaaa catttcaacc cttctctggc tcaaggagta
     5461 tctttaaaaa tgaaatactt tgcaatcagt attctgtagt taatagcgtt ctcttaatta
     5521 taatctgtga aatccaaagg agtgtctgtt attttcataa catataaatg gtccttctct
     5581 caaatcagct tcatcaaagg gaataaatga aagcttaagg ctgtttaata gacttcataa
     5641 cactgaattt cttttcatca tgatagtgac ttactaaaca aataatgtat ggcttggaaa
     5701 gttccagcag tcactaagga gttttatcta tttaaggtga tgcctttaaa gaggttattt
     5761 atgtgtatat gtgtttttgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgg
     5821 gttttttttt tccaaaggag attgataaag atacctttga gaatcttgct ccttagagta
     5881 tgaaaacgtt taaaggatag gaaaagctgc tactgaatca tgctttagga catatatcta
     5941 tagatactaa gaataactaa aatttgtact taagaatggt aacaacaaag tgtgtaacaa
     6001 tatgtgtgtg tattatatgt tttgtattta tgtataagta acttttttta aagctttttt
     6061 ttaaattgaa aaaagaagaa aatttatggc tcagttcctt ttttttaaaa tcttcactga
     6121 tccttcactt attactacaa tcttgccctc tttcattccc agacttggag tcccttatac
     6181 ctgcttcttt aatagccact ttccctaaat accctgtaat ttctgaggct gctgcttcta
     6241 ccagtgtaat acaagccatt gtcttttggt caaatctgag tttgttctcc tttctcatgt
     6301 tctttgtcct ctctgtgaca attgtatttt cttttatctt cccatcattt ccacaatggt
     6361 actgctcctt cctacatata cagttgaccc ttgagcaaca caagtttgaa ctgtgcaggt
     6421 ccacttatat gtggatctaa aatacagtat ttgtgggatg caaaatctga gtatatggag
     6481 gaccaacttt ttgtagaggc aggttcctca gggttaactg caggacttga gtaagtgcag
     6541 attatggtat gcacagggga tcctggaacc agtccccaga ccaaaaatga ccactgtatc
     6601 ttgagagcac cttcatggtc ttctttcctg tccccagtcg taagatacga cccttcatct
     6661 gctttgtctc aacattctgt tagtggtgaa ctgataactc cagcttttct ctctcttttt
     6721 tttttttttt ttttttttgt tgttgttgtt gttgttgttt tgaggcagag tcttgctctg
     6781 tcgcccaggc tggagtgcag tggcacgatc tcggctcact gcaagctctg cctcccgggt
     6841 tcacgccatt ctcctgcctc agcctcccaa gtagctggga ctacaggtgc ccgccaccac
     6901 gcccggctaa ttttttttgt atttttagta gagacggggt ttcaccatgt tgggctcgat
     6961 ctcctgacct catgatccgc ccgcctcggc ctcccaaagt gctgggatta caggcgtgat
     7021 gagccaccta gtgctaattc atctctaaac ctctgtagtt ggagtctcct gaaatctcag
     7081 actacctcct gctttcattt tctacccagg gaatacacta ttgtcctaag ttatttagcc
     7141 ttgaaatgtc agaggcattt taggttcttc atgtttctcc cactttcagt tgattaccat
     7201 tacaggatct taggcttctt tttatctttt tgcagtgcct tgcatccact ggttgtttgt
     7261 tttttctgtt gggcactact tatttcctta cctctggttt cttctcctac agcttatgtt
     7321 ctgaaatgtg tattcctaaa atactgtttt cctcttgtta cttttctgct cgaggacttc
     7381 cagtaactta ccattgttta taaggtgatg ttcaaaccct tcagtctgga gactttctgc
     7441 cacctatctg actgtacctt tatcttcaag cttatctctt tccaaacaag aggctcattt
     7501 agcagctttc tctgattgcc atgttcatcc tacactcagc ctcattcagt ttacagcatg
     7561 gaaactgtat aggacctctt tcctatagaa attgaagaca cttaaatagg aagaaaatta
     7621 aaatatacat ttggatacat gagtattcca gtcaaataat atctataaaa taccagatag
     7681 agtataaaag acaactgaag gacaacagag tgatgaaagg actttattag gcatttggat
     7741 ttggttatga tttaaatttc aatttaatta gaacgtttcc atggcaagga aggaagcatg
     7801 gaggactgtg gaaaagtcat tcagtattga gttcatttgc attagaggaa tttcatagtt
     7861 taaaacttgt atatctttac ctatccttcg tatgttttct tcttaagcat atttgacttt
     7921 ttctacctca gcatctgtat aagaaaatat ttgtgagtca gatgtttgtg ggttttcctt
     7981 acctattatt attttcttcc atgctttaca acacattttt taaactacct tgttcttaaa
     8041 taattacacg gacctgcttc tgtgtacttt cacagaatct ttgacagtta aaaattgtat
     8101 gttatataaa aatttgacaa gcttctacag ttaggaaaag cctttagaaa tctgccttcc
     8161 ccaaaccgta tgttatcata gcactcatgt ctccccatgt ctaaaaggta aatagataca
     8221 gaaacctcac caaaagttga atcatctgta ctaaaccact gcttttttat ccagactttt
     8281 tatataaatc ttttattttc caaaaaactg atcttttacc tacccctctt agagttaaaa
     8341 atattgcctg tcaggcaaga gtatagtatg ccaatataaa taatatgctt tatttggtta
     8401 gaaacagttg gtttgggaag ctagcaacat agcatattct ttacaaattt aataaggtgt
     8461 attttgatac tgtgataacc tgcattgtaa attaccaaat ggttggaatt gaccagttta
     8521 tacttattaa aatttttgat gtgccaatgg ctatccaatt cattgcattt gtgaattgtg
     8581 cttttagaaa aattgtggac attttagttt tatatacata tttatgagtt atttgtgcat
     8641 atgataaaat tatatataat aataaaagta tatatgtaac tta
//
