LOCUS       NM_001350600            8514 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens MMS22 like, DNA repair protein (MMS22L), transcript
            variant 3, mRNA.
ACCESSION   NM_001350600 XM_017010657
VERSION     NM_001350600.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   van Schie,J.J., de Lint,K., Pai,G.M., Rooimans,M.A., Wolthuis,R.M.
            and de Lange,J.
  TITLE     MMS22L-TONSL functions in sister chromatid cohesion in a pathway
            parallel to DSCC1-RFC
  JOURNAL   Life Sci Alliance 6 (2), e202201596 (2022)
   PUBMED   36622344
  REMARK    GeneRIF: MMS22L-TONSL functions in sister chromatid cohesion in a
            pathway parallel to DSCC1-RFC.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Huang,T.H., Fowler,F., Chen,C.C., Shen,Z.J., Sleckman,B. and
            Tyler,J.K.
  TITLE     The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated
            Rad51 Loading onto ssDNA during Homologous Recombination in Human
            Cells
  JOURNAL   Mol Cell 69 (5), 879-892 (2018)
   PUBMED   29478807
  REMARK    GeneRIF: uncovered DNA-PKcs-dependent DNA damage-induced ASF1A
            phosphorylation, which enhances chromatin assembly, promoting
            MMS22L-TONSL recruitment and, hence, Rad51 loading.
            Erratum:[Mol Cell. 2020 Mar 5;77(5):1153. doi:
            10.1016/j.molcel.2020.02.019.. PMID: 32142686]
REFERENCE   3  (bases 1 to 8514)
  AUTHORS   Schrors,B., Lubcke,S., Lennerz,V., Fatho,M., Bicker,A., Wolfel,C.,
            Derigs,P., Hankeln,T., Schadendorf,D., Paschen,A. and Wolfel,T.
  TITLE     HLA class I loss in metachronous metastases prevents continuous T
            cell recognition of mutated neoantigens in a human melanoma model
  JOURNAL   Oncotarget 8 (17), 28312-28327 (2017)
   PUBMED   28423700
REFERENCE   4  (bases 1 to 8514)
  AUTHORS   Piwko,W., Mlejnkova,L.J., Mutreja,K., Ranjha,L., Stafa,D.,
            Smirnov,A., Brodersen,M.M., Zellweger,R., Sturzenegger,A.,
            Janscak,P., Lopes,M., Peter,M. and Cejka,P.
  TITLE     The MMS22L-TONSL heterodimer directly promotes RAD51-dependent
            recombination upon replication stress
  JOURNAL   EMBO J 35 (23), 2584-2601 (2016)
   PUBMED   27797818
  REMARK    GeneRIF: By specifically regulating RAD51 activity at uncoupled
            replication forks, MMS22L-TONSL stabilizes perturbed replication
            forks by promoting replication fork reversal and stimulating their
            homologous recombination-mediated restart in vivo.
REFERENCE   5  (bases 1 to 8514)
  AUTHORS   Saredi,G., Huang,H., Hammond,C.M., Alabert,C., Bekker-Jensen,S.,
            Forne,I., Reveron-Gomez,N., Foster,B.M., Mlejnkova,L., Bartke,T.,
            Cejka,P., Mailand,N., Imhof,A., Patel,D.J. and Groth,A.
  TITLE     H4K20me0 marks post-replicative chromatin and recruits the
            TONSL-MMS22L DNA repair complex
  JOURNAL   Nature 534 (7609), 714-718 (2016)
   PUBMED   27338793
  REMARK    GeneRIF: new histones incorporated during DNA replication provide a
            signature of post-replicative chromatin, read by the human
            TONSL-MMS22L homologous recombination complex
REFERENCE   6  (bases 1 to 8514)
  AUTHORS   Piwko,W., Buser,R. and Peter,M.
  TITLE     Rescuing stalled replication forks: MMS22L-TONSL, a novel complex
            for DNA replication fork repair in human cells
  JOURNAL   Cell Cycle 10 (11), 1703-1705 (2011)
   PUBMED   21519189
REFERENCE   7  (bases 1 to 8514)
  AUTHORS   Piwko,W., Olma,M.H., Held,M., Bianco,J.N., Pedrioli,P.G.,
            Hofmann,K., Pasero,P., Gerlich,D.W. and Peter,M.
  TITLE     RNAi-based screening identifies the Mms22L-Nfkbil2 complex as a
            novel regulator of DNA replication in human cells
  JOURNAL   EMBO J 29 (24), 4210-4222 (2010)
   PUBMED   21113133
  REMARK    GeneRIF: Results strongly suggest that the Mms22L-Nfkbil2 complex
            contributes to genome stability by regulating the chromatin state
            at stalled replication forks.
REFERENCE   8  (bases 1 to 8514)
  AUTHORS   O'Connell,B.C., Adamson,B., Lydeard,J.R., Sowa,M.E., Ciccia,A.,
            Bredemeyer,A.L., Schlabach,M., Gygi,S.P., Elledge,S.J. and
            Harper,J.W.
  TITLE     A genome-wide camptothecin sensitivity screen identifies a
            mammalian MMS22L-NFKBIL2 complex required for genomic stability
  JOURNAL   Mol Cell 40 (4), 645-657 (2010)
   PUBMED   21055985
  REMARK    GeneRIF: This study identifies MMS22L-NFKBIL2 as components of the
            replication stress control pathway.
REFERENCE   9  (bases 1 to 8514)
  AUTHORS   Duro,E., Lundin,C., Ask,K., Sanchez-Pulido,L., MacArtney,T.J.,
            Toth,R., Ponting,C.P., Groth,A., Helleday,T. and Rouse,J.
  TITLE     Identification of the MMS22L-TONSL complex that promotes homologous
            recombination
  JOURNAL   Mol Cell 40 (4), 632-644 (2010)
   PUBMED   21055984
  REMARK    GeneRIF: MMS22L and TONSL are required for the maintenance of
            genome stability.
REFERENCE   10 (bases 1 to 8514)
  AUTHORS   O'Donnell,L., Panier,S., Wildenhain,J., Tkach,J.M., Al-Hakim,A.,
            Landry,M.C., Escribano-Diaz,C., Szilard,R.K., Young,J.T., Munro,M.,
            Canny,M.D., Kolas,N.K., Zhang,W., Harding,S.M., Ylanko,J.,
            Mendez,M., Mullin,M., Sun,T., Habermann,B., Datti,A., Bristow,R.G.,
            Gingras,A.C., Tyers,M.D., Brown,G.W. and Durocher,D.
  TITLE     The MMS22L-TONSL complex mediates recovery from replication stress
            and homologous recombination
  JOURNAL   Mol Cell 40 (4), 619-631 (2010)
   PUBMED   21055983
  REMARK    GeneRIF: These results indicate that MMS22L and TONSL are genome
            caretakers that stimulate the recombination-dependent repair of
            stalled or collapsed replication forks.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353679.18, AL590484.10 and
            AL023656.8.
            
            On Jun 2, 2019 this sequence version replaced NM_001350600.1.
            
            Summary: The protein encoded by this gene forms a complex with
            tonsoku-like, DNA repair protein (TONSL), and this complex
            recognizes and repairs DNA double-strand breaks at sites of stalled
            or collapsed replication forks. The encoded protein also can bind
            with the histone-associated protein NFKBIL2 to help regulate the
            chromatin state at stalled replication forks. Finally, this gene
            appears to be overexpressed in most lung and esophageal cancers.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Apr 2017].
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (b) is
            shorter at the N-terminus compared to isoform a.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.102715.1,
                                           SRR18074967.525325.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               AL353679.18        13485-13605         c
            122-361             AL353679.18        12703-12942         c
            362-487             AL353679.18        11626-11751         c
            488-575             AL353679.18        3364-3451           c
            576-753             AL353679.18        3093-3270           c
            754-844             AL353679.18        291-381             c
            845-965             AL590484.10        63411-63531         c
            966-1079            AL590484.10        58864-58977         c
            1080-1256           AL590484.10        50086-50262         c
            1257-1319           AL590484.10        42157-42219         c
            1320-1439           AL590484.10        29390-29509         c
            1440-1666           AL590484.10        26955-27181         c
            1667-2176           AL590484.10        24423-24932         c
            2177-2370           AL023656.8         94287-94480         c
            2371-2521           AL023656.8         89694-89844         c
            2522-2673           AL023656.8         87198-87349         c
            2674-2816           AL023656.8         86233-86375         c
            2817-2976           AL023656.8         80853-81012         c
            2977-3146           AL023656.8         75861-76030         c
            3147-3358           AL023656.8         73036-73247         c
            3359-3522           AL023656.8         69792-69955         c
            3523-3619           AL023656.8         59561-59657         c
            3620-3787           AL023656.8         57643-57810         c
            3788-8514           AL023656.8         49951-54677         c
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q16.1"
     gene            1..8514
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="MMS22 like, DNA repair protein"
                     /db_xref="GeneID:253714"
                     /db_xref="HGNC:HGNC:21475"
                     /db_xref="MIM:615614"
     exon            1..121
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            122..361
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            362..487
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            488..575
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            576..753
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            754..844
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            845..965
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1079
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     CDS             987..3869
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="isoform b is encoded by transcript variant 3"
                     /codon_start=1
                     /product="protein MMS22-like isoform b"
                     /protein_id="NP_001337529.1"
                     /db_xref="GeneID:253714"
                     /db_xref="HGNC:HGNC:21475"
                     /db_xref="MIM:615614"
                     /translation="MSDQCPCLCIKELWVLLIHLLDHRSKWFVSESFWNWLNKLLKTL
                     LEKSSDRRRSSMPVIQSRDPLGFSWWIITHVASFYKFDRHGVPDEMRKVESNWNFVEE
                     LLKKSISVQGVILEEQLRMYLHCCLTLCDFWEPNIAIVTILWEYYSKNLNSSFSISWL
                     PFKGLANTMKSPLSMLEMVKTCCCDKQDQELYKSSSSYTIFLCILAKVVKKAMKSNGP
                     HPWKQVKGRIYSKFHQKRMEELTEVGLQNFFSLFLLLAAVAEVEDVASHVLDLLNFLK
                     PAFVTSQRALIWKGHMAFLLMYAQKNLDIGVLAEKFSCAFREKAKEFLVSKNEEMVQR
                     QTIWTLLSIYIDGVQEVFETSYCLYPSHEKLLNDGFSMLLRACRESELRTVLSFLQAV
                     LARIRSMHQQLCQELQRDNVDLFVQSSLSAKERHLAAVASALWRHFFSFLKSQRMSQV
                     VPFSQLADAAADFTLLAMDMPSTAPSDFQPQPVISIIQLFGWDDIICPQVVARYLSHV
                     LQNSTLCEALSHSGYVSFQALTVRSWIRCVLQMYIKNLSGPDDLLIDKNLEEAVEKEY
                     MKQLVKLTRLLFNLSEVKSIFSKAQVEYLSISEDPKKALVRFFEAVGVTYGNVQTLSD
                     KSAMVTKSLEYLGEVLKYIKPYLGKKVFSAGLQLTYGMMGILVKSWAQIFATSKAQKL
                     LFRIIDCLLLPHAVLQQEKELPAPMLSAIQKSLPLYLQGMCIVCCQSQNPNAYLNQLL
                     GNVIEQYIGRFLPASPYVSDLGQHPVLLALRNTATIPPISSLKKCIVQVIRKSYLEYK
                     GSSPPPRLASILAFILQLFKETNTDIYEVELLLPGILKCLVLVSEPQVKRLATENLQY
                     MVKACQVGSEEEPSSQLTSVFRQFIQDYGMRYYYQVYSILETVATLDQQVVIHLISTL
                     TQSLKDSEQKWGLGRNIAQREAYSKLLSHLGQMGQDEMQRLENDNT"
     exon            1080..1256
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1319
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1439
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1666
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..2176
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2370
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2371..2521
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2522..2673
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2674..2816
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2817..2976
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..3146
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3147..3358
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3359..3522
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3523..3619
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3787
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3788..8514
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4120..4125
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="hexamer: ATTAAA"
     polyA_site      4141
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="major polyA site"
     regulatory      8492..8497
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="hexamer: AATAAA"
     polyA_site      8514
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="major polyA site"
ORIGIN      
        1 ctttccggag cgcgggcgcg cggtggcggg aatttcgcct gtttgcggtt tagaccccaa
       61 agattcctgt tggtggtctg ggtcacagga ggcaggtttc gggagctgga aatgtgagcg
      121 ggaatctctt cacaccttct ctttggagcc cttaatgata cgacgaaccc caagtgtttc
      181 agaacatgaa gtaaacaatg gagaactgtt ctgctgcatc gacgttcctg actgacagct
      241 tagagctgga gctggggacg gaatggtgca aacctcctta cttttcttgt gctgttgaca
      301 acagaggagg aggaaaacat ttttctggag aatcctacct ctgcagcgga gcccttaagc
      361 gattgatttt gaatcttgac cctttaccaa ctaattttga agaagatacc ttggaaatat
      421 ttggcattca gtgggttact gaaacagcat tagtgaattc atctagagaa ctctttcatt
      481 tattcaggga aggtatcaac tctacactgc aaagcagaca atattaggca gcagtgtgta
      541 ctatttctcc attatgttaa agttttcatc ttcaggtatc tgaaagtaca gaatgctgag
      601 agtcatgttc ctgtccatcc ttatgaggct ttggaggctc agcttccctc agtgttgatt
      661 gatgagcttc atggattact cttgtatatt ggacacctat ctgaacttcc cagtgttaat
      721 ataggagcat ttgtaaatca aaaccagatt aagctttttc caccgtcatg gcatttatta
      781 catctccact tggatataca ttggctggtg ctagaaattc tttacatgct gggtgaaaaa
      841 ttgaaacaag ttgtatatgg tcatcagttt atgaatctgg caagtgacaa tttaaccaac
      901 atcagcctat ttgaagaaca ttgtgaaact ctcctttgtg atttaataag cctgtcactc
      961 aacaggttag gtcttctgaa tcattaatga gtgaccagtg tccatgttta tgcattaaag
     1021 aattatgggt tctacttatt catcttctag accacagaag taaatggttt gtctcggaat
     1081 cattttggaa ctggttgaat aaactactta aaacactgct tgaaaaatca agtgaccgaa
     1141 gaagatcctc tatgcctgta atccagtcca gggatccatt aggttttagt tggtggatta
     1201 ttactcatgt agcatcattt tacaagtttg atcgccatgg agtaccagat gaaatgagaa
     1261 aagtggaatc aaattggaac tttgtagaag aactgctgaa aaagtccatc agtgttcagg
     1321 gtgtcattct agaagaacaa ttacgaatgt atcttcactg ttgtttgaca ctttgtgatt
     1381 tctgggagcc aaacattgca attgttacca ttttatggga atattatagt aagaacctga
     1441 atagttcctt cagtatttct tggcttcctt ttaaaggcct tgctaatacc atgaagtcac
     1501 ccttgtctat gcttgaaatg gtgaagactt gctgttgcga taaacaagat caggaactat
     1561 ataaatccag cagtagttat actatttttc tttgtattct ggcaaaagtt gttaaaaaag
     1621 caatgaagag caatggccct catccttgga aacaagtcaa aggaagaata tattcaaaat
     1681 tccatcaaaa aagaatggaa gaactaactg aagttggtct acagaacttt tttagccttt
     1741 ttctactgtt agcagctgtt gcagaggtag aagatgttgc aagtcatgtt ttagacctcc
     1801 tgaatttcct caagcctgct tttgtaacgt ctcagagagc cctcatttgg aagggtcaca
     1861 tggccttcct cttgatgtat gcccagaaaa atctggacat tggtgttttg gctgagaaat
     1921 tttcatgtgc tttccgggag aaagcaaagg aattcttggt gtctaagaat gaggaaatgg
     1981 tacagagaca gactatctgg acccttcttt ccatatacat tgatggtgtt caagaagtgt
     2041 ttgagaccag ctattgcttg tatccttccc atgaaaaact gcttaatgat ggatttagta
     2101 tgcttctgcg agcatgtcga gaatctgaac ttaggacagt attgagcttc ctacaagctg
     2161 ttctggccag aatcaggagt atgcatcaac aattgtgtca ggaacttcaa agggacaatg
     2221 tggacctatt tgtacagtct tcattatcgg ctaaagagcg ccaccttgct gcagttgcca
     2281 gtgcactgtg gagacatttc ttttcatttt tgaagagtca gagaatgtca caggtagtgc
     2341 ctttctcaca acttgcggat gcagctgcag actttacttt gctagcaatg gacatgccaa
     2401 gcacagctcc atcagatttt cagcctcagc cagttatatc aattattcaa ctttttggtt
     2461 gggatgatat catctgccct caagttgtag caagatattt aagtcatgtc ctacaaaata
     2521 gcacattatg tgaagcactt tctcattcag gctatgtatc ttttcaagcc ttaaccgtaa
     2581 gatcatggat tcgttgtgtt ttgcaaatgt atattaaaaa cctctctggg cctgatgatt
     2641 tgctcataga taaaaatctg gaagaggcag ttgaaaaaga gtacatgaaa cagttggtca
     2701 aactgacaag attactattt aatctctcag aagtaaagag tattttctca aaggcccaag
     2761 ttgaatattt atccatctca gaagacccta aaaaagcact tgttcgattc tttgaggctg
     2821 ttggtgtaac ttacgggaac gtccagacac tttctgataa atctgccatg gtcacaaagt
     2881 ccttggaata ccttggtgaa gtattaaaat atattaagcc ttatttggga aaaaaagttt
     2941 tcagtgcagg gctgcagctg acttatggaa tgatgggaat tcttgtgaaa tcatgggcac
     3001 aaatctttgc cacttctaaa gcccaaaaat tactattccg gatcatagat tgtttactgc
     3061 tgccacatgc agtattacag caagagaagg aactgcctgc acctatgttg tcagcaattc
     3121 agaaaagtct tcctttgtat ctccagggca tgtgtatcgt gtgttgtcaa tctcaaaatc
     3181 cgaatgccta tttgaatcaa ttgctaggga atgttattga gcagtatatt gggcgatttc
     3241 ttccagcttc accatatgtt tcagatcttg gacaacatcc tgttttgctg gcattgagaa
     3301 acacagccac tattccacca atatcatctc taaagaaatg cattgtgcaa gtcataagga
     3361 aatcctacct tgagtataag gggtcctcac ctcctcctcg cttagcatcc attctggcct
     3421 tcatcctcca actcttcaag gaaactaaca cagacattta tgaagttgaa ctactcctcc
     3481 ctggcatttt aaaatgcttg gtgttagtca gtgaaccaca agttaaaagg ctggccacag
     3541 agaacctgca atacatggta aaagcctgcc aagtggggtc agaagaagaa ccttcctccc
     3601 agctgacttc tgtgtttagg cagtttatcc aggattatgg tatgaggtac tattaccagg
     3661 tttacagcat tttagaaaca gtagcaacat tggaccagca ggttgtcatc cacttgattt
     3721 ctacccttac tcagtctctg aaggattcag agcagaaatg gggccttggc aggaatatag
     3781 cacaaaggga agcctatagc aaacttttgt ctcaccttgg acagatggga caagatgaga
     3841 tgcagagact ggaaaatgat aatacttaat atattttgtg attcatcctg tctattaatt
     3901 atcagtatct aaagccacat tgttccactc taattcctac ttttaattaa ttgtataatg
     3961 ttctgtttaa aataatttct aggatttttt ttccttttat atctgtaagt aaaaaatgta
     4021 taaataagga actgaaggtt aattagcaac tgtgtaatag gatccttgga actttttaca
     4081 aaactgcatt ctaccttgat gaaattttag ttaaactaaa ttaaagaaac attttctgag
     4141 agtgttttct gtgtgtgagt attaagcaga tacttcatcc atagactttt tgttgtagta
     4201 tgacttcctg cctccctgcc ttgtgactga tttgtcatgt ttatttttta tttttacaat
     4261 aatagttatg ttttaaaatg atactttttc aaagtacctg gctaccctat actgtgtttt
     4321 tggtggggga aaggtgttgg gaaatgtgac tgtgtatttg tttttctttt ttatatgtgt
     4381 gaggcaagtt aacaaagaaa acccttacaa agcacactta actgtgttag gaatgagact
     4441 gaatctggct aaaggattat acagctgata tccaaaaaga agtgtaaaca tgccaacagg
     4501 gtttatattt aggttccaag agttgccaaa tttttttttt attcatcctt tcttttggag
     4561 ttgtcattaa aaaaaaaatc atatcaggag gaggaaataa aggagacttt aagaggagag
     4621 taaataaaaa tattctagaa aagtaacaat agtagtggtc tgagaagtca gctgatccat
     4681 cttcttgctc tttagccaga gcttcttata acttagcatt ttatccaaga aacctgttta
     4741 gtttccttta acaacattgt ttctcaactt ttgaactctt aactcatcca gattgtttta
     4801 ccagttggag cttgtccttg actccttttc ctattgagca ttatttggtt gaaagagctc
     4861 cagtcctagt tttgggaacc cacctgggtc tcaaactcag ctttgcttta tgatcttgag
     4921 ctagttacgt aacctttctc agtctagttt cttcatggag aagaaaagaa gaatcccttt
     4981 actatttgac agtgttgttg tgagaatcaa tgggataatg tacgtgaaaa tatttggtgg
     5041 cccaaattct gttgggaaat gtacagacac gattagttta ttactcgtgc acaattaagg
     5101 caaagaacac ctctcaattt tatctttctc taatgatatg tcccagatga atgatcccac
     5161 tttccaatgt tttcttacat catgttttct aactttgaag tactttgttt ccttcttggg
     5221 attatagtct ttatcatggt tctactcttc cttcaagaaa acatttcaac ccttctctgg
     5281 ctcaaggagt atctttaaaa atgaaatact ttgcaatcag tattctgtag ttaatagcgt
     5341 tctcttaatt ataatctgtg aaatccaaag gagtgtctgt tattttcata acatataaat
     5401 ggtccttctc tcaaatcagc ttcatcaaag ggaataaatg aaagcttaag gctgtttaat
     5461 agacttcata acactgaatt tcttttcatc atgatagtga cttactaaac aaataatgta
     5521 tggcttggaa agttccagca gtcactaagg agttttatct atttaaggtg atgcctttaa
     5581 agaggttatt tatgtgtata tgtgtttttg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     5641 tgtgtgtgtg ggtttttttt ttccaaagga gattgataaa gatacctttg agaatcttgc
     5701 tccttagagt atgaaaacgt ttaaaggata ggaaaagctg ctactgaatc atgctttagg
     5761 acatatatct atagatacta agaataacta aaatttgtac ttaagaatgg taacaacaaa
     5821 gtgtgtaaca atatgtgtgt gtattatatg ttttgtattt atgtataagt aacttttttt
     5881 aaagcttttt tttaaattga aaaaagaaga aaatttatgg ctcagttcct tttttttaaa
     5941 atcttcactg atccttcact tattactaca atcttgccct ctttcattcc cagacttgga
     6001 gtcccttata cctgcttctt taatagccac tttccctaaa taccctgtaa tttctgaggc
     6061 tgctgcttct accagtgtaa tacaagccat tgtcttttgg tcaaatctga gtttgttctc
     6121 ctttctcatg ttctttgtcc tctctgtgac aattgtattt tcttttatct tcccatcatt
     6181 tccacaatgg tactgctcct tcctacatat acagttgacc cttgagcaac acaagtttga
     6241 actgtgcagg tccacttata tgtggatcta aaatacagta tttgtgggat gcaaaatctg
     6301 agtatatgga ggaccaactt tttgtagagg caggttcctc agggttaact gcaggacttg
     6361 agtaagtgca gattatggta tgcacagggg atcctggaac cagtccccag accaaaaatg
     6421 accactgtat cttgagagca ccttcatggt cttctttcct gtccccagtc gtaagatacg
     6481 acccttcatc tgctttgtct caacattctg ttagtggtga actgataact ccagcttttc
     6541 tctctctttt tttttttttt tttttttttg ttgttgttgt tgttgttgtt ttgaggcaga
     6601 gtcttgctct gtcgcccagg ctggagtgca gtggcacgat ctcggctcac tgcaagctct
     6661 gcctcccggg ttcacgccat tctcctgcct cagcctccca agtagctggg actacaggtg
     6721 cccgccacca cgcccggcta attttttttg tatttttagt agagacgggg tttcaccatg
     6781 ttgggctcga tctcctgacc tcatgatccg cccgcctcgg cctcccaaag tgctgggatt
     6841 acaggcgtga tgagccacct agtgctaatt catctctaaa cctctgtagt tggagtctcc
     6901 tgaaatctca gactacctcc tgctttcatt ttctacccag ggaatacact attgtcctaa
     6961 gttatttagc cttgaaatgt cagaggcatt ttaggttctt catgtttctc ccactttcag
     7021 ttgattacca ttacaggatc ttaggcttct ttttatcttt ttgcagtgcc ttgcatccac
     7081 tggttgtttg ttttttctgt tgggcactac ttatttcctt acctctggtt tcttctccta
     7141 cagcttatgt tctgaaatgt gtattcctaa aatactgttt tcctcttgtt acttttctgc
     7201 tcgaggactt ccagtaactt accattgttt ataaggtgat gttcaaaccc ttcagtctgg
     7261 agactttctg ccacctatct gactgtacct ttatcttcaa gcttatctct ttccaaacaa
     7321 gaggctcatt tagcagcttt ctctgattgc catgttcatc ctacactcag cctcattcag
     7381 tttacagcat ggaaactgta taggacctct ttcctataga aattgaagac acttaaatag
     7441 gaagaaaatt aaaatataca tttggataca tgagtattcc agtcaaataa tatctataaa
     7501 ataccagata gagtataaaa gacaactgaa ggacaacaga gtgatgaaag gactttatta
     7561 ggcatttgga tttggttatg atttaaattt caatttaatt agaacgtttc catggcaagg
     7621 aaggaagcat ggaggactgt ggaaaagtca ttcagtattg agttcatttg cattagagga
     7681 atttcatagt ttaaaacttg tatatcttta cctatccttc gtatgttttc ttcttaagca
     7741 tatttgactt tttctacctc agcatctgta taagaaaata tttgtgagtc agatgtttgt
     7801 gggttttcct tacctattat tattttcttc catgctttac aacacatttt ttaaactacc
     7861 ttgttcttaa ataattacac ggacctgctt ctgtgtactt tcacagaatc tttgacagtt
     7921 aaaaattgta tgttatataa aaatttgaca agcttctaca gttaggaaaa gcctttagaa
     7981 atctgccttc cccaaaccgt atgttatcat agcactcatg tctccccatg tctaaaaggt
     8041 aaatagatac agaaacctca ccaaaagttg aatcatctgt actaaaccac tgctttttta
     8101 tccagacttt ttatataaat cttttatttt ccaaaaaact gatcttttac ctacccctct
     8161 tagagttaaa aatattgcct gtcaggcaag agtatagtat gccaatataa ataatatgct
     8221 ttatttggtt agaaacagtt ggtttgggaa gctagcaaca tagcatattc tttacaaatt
     8281 taataaggtg tattttgata ctgtgataac ctgcattgta aattaccaaa tggttggaat
     8341 tgaccagttt atacttatta aaatttttga tgtgccaatg gctatccaat tcattgcatt
     8401 tgtgaattgt gcttttagaa aaattgtgga cattttagtt ttatatacat atttatgagt
     8461 tatttgtgca tatgataaaa ttatatataa taataaaagt atatatgtaa ctta
//
