LOCUS       NM_001350599            8574 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens MMS22 like, DNA repair protein (MMS22L), transcript
            variant 2, mRNA.
ACCESSION   NM_001350599 XM_006715430
VERSION     NM_001350599.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   van Schie,J.J., de Lint,K., Pai,G.M., Rooimans,M.A., Wolthuis,R.M.
            and de Lange,J.
  TITLE     MMS22L-TONSL functions in sister chromatid cohesion in a pathway
            parallel to DSCC1-RFC
  JOURNAL   Life Sci Alliance 6 (2), e202201596 (2022)
   PUBMED   36622344
  REMARK    GeneRIF: MMS22L-TONSL functions in sister chromatid cohesion in a
            pathway parallel to DSCC1-RFC.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Huang,T.H., Fowler,F., Chen,C.C., Shen,Z.J., Sleckman,B. and
            Tyler,J.K.
  TITLE     The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated
            Rad51 Loading onto ssDNA during Homologous Recombination in Human
            Cells
  JOURNAL   Mol Cell 69 (5), 879-892 (2018)
   PUBMED   29478807
  REMARK    GeneRIF: uncovered DNA-PKcs-dependent DNA damage-induced ASF1A
            phosphorylation, which enhances chromatin assembly, promoting
            MMS22L-TONSL recruitment and, hence, Rad51 loading.
            Erratum:[Mol Cell. 2020 Mar 5;77(5):1153. doi:
            10.1016/j.molcel.2020.02.019.. PMID: 32142686]
REFERENCE   3  (bases 1 to 8574)
  AUTHORS   Schrors,B., Lubcke,S., Lennerz,V., Fatho,M., Bicker,A., Wolfel,C.,
            Derigs,P., Hankeln,T., Schadendorf,D., Paschen,A. and Wolfel,T.
  TITLE     HLA class I loss in metachronous metastases prevents continuous T
            cell recognition of mutated neoantigens in a human melanoma model
  JOURNAL   Oncotarget 8 (17), 28312-28327 (2017)
   PUBMED   28423700
REFERENCE   4  (bases 1 to 8574)
  AUTHORS   Piwko,W., Mlejnkova,L.J., Mutreja,K., Ranjha,L., Stafa,D.,
            Smirnov,A., Brodersen,M.M., Zellweger,R., Sturzenegger,A.,
            Janscak,P., Lopes,M., Peter,M. and Cejka,P.
  TITLE     The MMS22L-TONSL heterodimer directly promotes RAD51-dependent
            recombination upon replication stress
  JOURNAL   EMBO J 35 (23), 2584-2601 (2016)
   PUBMED   27797818
  REMARK    GeneRIF: By specifically regulating RAD51 activity at uncoupled
            replication forks, MMS22L-TONSL stabilizes perturbed replication
            forks by promoting replication fork reversal and stimulating their
            homologous recombination-mediated restart in vivo.
REFERENCE   5  (bases 1 to 8574)
  AUTHORS   Saredi,G., Huang,H., Hammond,C.M., Alabert,C., Bekker-Jensen,S.,
            Forne,I., Reveron-Gomez,N., Foster,B.M., Mlejnkova,L., Bartke,T.,
            Cejka,P., Mailand,N., Imhof,A., Patel,D.J. and Groth,A.
  TITLE     H4K20me0 marks post-replicative chromatin and recruits the
            TONSL-MMS22L DNA repair complex
  JOURNAL   Nature 534 (7609), 714-718 (2016)
   PUBMED   27338793
  REMARK    GeneRIF: new histones incorporated during DNA replication provide a
            signature of post-replicative chromatin, read by the human
            TONSL-MMS22L homologous recombination complex
REFERENCE   6  (bases 1 to 8574)
  AUTHORS   Piwko,W., Buser,R. and Peter,M.
  TITLE     Rescuing stalled replication forks: MMS22L-TONSL, a novel complex
            for DNA replication fork repair in human cells
  JOURNAL   Cell Cycle 10 (11), 1703-1705 (2011)
   PUBMED   21519189
REFERENCE   7  (bases 1 to 8574)
  AUTHORS   Piwko,W., Olma,M.H., Held,M., Bianco,J.N., Pedrioli,P.G.,
            Hofmann,K., Pasero,P., Gerlich,D.W. and Peter,M.
  TITLE     RNAi-based screening identifies the Mms22L-Nfkbil2 complex as a
            novel regulator of DNA replication in human cells
  JOURNAL   EMBO J 29 (24), 4210-4222 (2010)
   PUBMED   21113133
  REMARK    GeneRIF: Results strongly suggest that the Mms22L-Nfkbil2 complex
            contributes to genome stability by regulating the chromatin state
            at stalled replication forks.
REFERENCE   8  (bases 1 to 8574)
  AUTHORS   O'Connell,B.C., Adamson,B., Lydeard,J.R., Sowa,M.E., Ciccia,A.,
            Bredemeyer,A.L., Schlabach,M., Gygi,S.P., Elledge,S.J. and
            Harper,J.W.
  TITLE     A genome-wide camptothecin sensitivity screen identifies a
            mammalian MMS22L-NFKBIL2 complex required for genomic stability
  JOURNAL   Mol Cell 40 (4), 645-657 (2010)
   PUBMED   21055985
  REMARK    GeneRIF: This study identifies MMS22L-NFKBIL2 as components of the
            replication stress control pathway.
REFERENCE   9  (bases 1 to 8574)
  AUTHORS   Duro,E., Lundin,C., Ask,K., Sanchez-Pulido,L., MacArtney,T.J.,
            Toth,R., Ponting,C.P., Groth,A., Helleday,T. and Rouse,J.
  TITLE     Identification of the MMS22L-TONSL complex that promotes homologous
            recombination
  JOURNAL   Mol Cell 40 (4), 632-644 (2010)
   PUBMED   21055984
  REMARK    GeneRIF: MMS22L and TONSL are required for the maintenance of
            genome stability.
REFERENCE   10 (bases 1 to 8574)
  AUTHORS   O'Donnell,L., Panier,S., Wildenhain,J., Tkach,J.M., Al-Hakim,A.,
            Landry,M.C., Escribano-Diaz,C., Szilard,R.K., Young,J.T., Munro,M.,
            Canny,M.D., Kolas,N.K., Zhang,W., Harding,S.M., Ylanko,J.,
            Mendez,M., Mullin,M., Sun,T., Habermann,B., Datti,A., Bristow,R.G.,
            Gingras,A.C., Tyers,M.D., Brown,G.W. and Durocher,D.
  TITLE     The MMS22L-TONSL complex mediates recovery from replication stress
            and homologous recombination
  JOURNAL   Mol Cell 40 (4), 619-631 (2010)
   PUBMED   21055983
  REMARK    GeneRIF: These results indicate that MMS22L and TONSL are genome
            caretakers that stimulate the recombination-dependent repair of
            stalled or collapsed replication forks.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353679.18, AL590484.10 and
            AL023656.8.
            
            On Jun 2, 2019 this sequence version replaced NM_001350599.1.
            
            Summary: The protein encoded by this gene forms a complex with
            tonsoku-like, DNA repair protein (TONSL), and this complex
            recognizes and repairs DNA double-strand breaks at sites of stalled
            or collapsed replication forks. The encoded protein also can bind
            with the histone-associated protein NFKBIL2 to help regulate the
            chromatin state at stalled replication forks. Finally, this gene
            appears to be overexpressed in most lung and esophageal cancers.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Apr 2017].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.2440007.1,
                                           SRR14038196.2708671.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000683635.1/ ENSP00000508046.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               AL353679.18        13485-13605         c
            122-361             AL353679.18        12703-12942         c
            362-487             AL353679.18        11626-11751         c
            488-537             AL353679.18        9238-9287           c
            538-625             AL353679.18        3364-3451           c
            626-803             AL353679.18        3093-3270           c
            804-894             AL353679.18        291-381             c
            895-1025            AL590484.10        63401-63531         c
            1026-1139           AL590484.10        58864-58977         c
            1140-1316           AL590484.10        50086-50262         c
            1317-1379           AL590484.10        42157-42219         c
            1380-1499           AL590484.10        29390-29509         c
            1500-1726           AL590484.10        26955-27181         c
            1727-2236           AL590484.10        24423-24932         c
            2237-2430           AL023656.8         94287-94480         c
            2431-2581           AL023656.8         89694-89844         c
            2582-2733           AL023656.8         87198-87349         c
            2734-2876           AL023656.8         86233-86375         c
            2877-3036           AL023656.8         80853-81012         c
            3037-3206           AL023656.8         75861-76030         c
            3207-3418           AL023656.8         73036-73247         c
            3419-3582           AL023656.8         69792-69955         c
            3583-3679           AL023656.8         59561-59657         c
            3680-3847           AL023656.8         57643-57810         c
            3848-8574           AL023656.8         49951-54677         c
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q16.1"
     gene            1..8574
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="MMS22 like, DNA repair protein"
                     /db_xref="GeneID:253714"
                     /db_xref="HGNC:HGNC:21475"
                     /db_xref="MIM:615614"
     exon            1..121
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            122..361
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     CDS             198..3929
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="isoform a is encoded by transcript variant 2"
                     /codon_start=1
                     /product="protein MMS22-like isoform a"
                     /protein_id="NP_001337528.1"
                     /db_xref="CCDS:CCDS5039.1"
                     /db_xref="GeneID:253714"
                     /db_xref="HGNC:HGNC:21475"
                     /db_xref="MIM:615614"
                     /translation="MENCSAASTFLTDSLELELGTEWCKPPYFSCAVDNRGGGKHFSG
                     ESYLCSGALKRLILNLDPLPTNFEEDTLEIFGIQWVTETALVNSSRELFHLFRQQLYN
                     LETLLQSSCDFGKVSTLHCKADNIRQQCVLFLHYVKVFIFRYLKVQNAESHVPVHPYE
                     ALEAQLPSVLIDELHGLLLYIGHLSELPSVNIGAFVNQNQIKLFPPSWHLLHLHLDIH
                     WLVLEILYMLGEKLKQVVYGHQFMNLASDNLTNISLFEEHCETLLCDLISLSLNRYDK
                     VRSSESLMSDQCPCLCIKELWVLLIHLLDHRSKWFVSESFWNWLNKLLKTLLEKSSDR
                     RRSSMPVIQSRDPLGFSWWIITHVASFYKFDRHGVPDEMRKVESNWNFVEELLKKSIS
                     VQGVILEEQLRMYLHCCLTLCDFWEPNIAIVTILWEYYSKNLNSSFSISWLPFKGLAN
                     TMKSPLSMLEMVKTCCCDKQDQELYKSSSSYTIFLCILAKVVKKAMKSNGPHPWKQVK
                     GRIYSKFHQKRMEELTEVGLQNFFSLFLLLAAVAEVEDVASHVLDLLNFLKPAFVTSQ
                     RALIWKGHMAFLLMYAQKNLDIGVLAEKFSCAFREKAKEFLVSKNEEMVQRQTIWTLL
                     SIYIDGVQEVFETSYCLYPSHEKLLNDGFSMLLRACRESELRTVLSFLQAVLARIRSM
                     HQQLCQELQRDNVDLFVQSSLSAKERHLAAVASALWRHFFSFLKSQRMSQVVPFSQLA
                     DAAADFTLLAMDMPSTAPSDFQPQPVISIIQLFGWDDIICPQVVARYLSHVLQNSTLC
                     EALSHSGYVSFQALTVRSWIRCVLQMYIKNLSGPDDLLIDKNLEEAVEKEYMKQLVKL
                     TRLLFNLSEVKSIFSKAQVEYLSISEDPKKALVRFFEAVGVTYGNVQTLSDKSAMVTK
                     SLEYLGEVLKYIKPYLGKKVFSAGLQLTYGMMGILVKSWAQIFATSKAQKLLFRIIDC
                     LLLPHAVLQQEKELPAPMLSAIQKSLPLYLQGMCIVCCQSQNPNAYLNQLLGNVIEQY
                     IGRFLPASPYVSDLGQHPVLLALRNTATIPPISSLKKCIVQVIRKSYLEYKGSSPPPR
                     LASILAFILQLFKETNTDIYEVELLLPGILKCLVLVSEPQVKRLATENLQYMVKACQV
                     GSEEEPSSQLTSVFRQFIQDYGMRYYYQVYSILETVATLDQQVVIHLISTLTQSLKDS
                     EQKWGLGRNIAQREAYSKLLSHLGQMGQDEMQRLENDNT"
     exon            362..487
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            488..537
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            538..625
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            626..803
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            804..894
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1025
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1139
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1316
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1379
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1499
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1726
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..2236
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2430
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2431..2581
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2582..2733
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2876
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            2877..3036
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..3206
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3207..3418
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3419..3582
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3583..3679
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3680..3847
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     exon            3848..8574
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4180..4185
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="hexamer: ATTAAA"
     polyA_site      4201
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="major polyA site"
     regulatory      8552..8557
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="hexamer: AATAAA"
     polyA_site      8574
                     /gene="MMS22L"
                     /gene_synonym="C6orf167; dJ39B17.2"
                     /note="major polyA site"
ORIGIN      
        1 ctttccggag cgcgggcgcg cggtggcggg aatttcgcct gtttgcggtt tagaccccaa
       61 agattcctgt tggtggtctg ggtcacagga ggcaggtttc gggagctgga aatgtgagcg
      121 ggaatctctt cacaccttct ctttggagcc cttaatgata cgacgaaccc caagtgtttc
      181 agaacatgaa gtaaacaatg gagaactgtt ctgctgcatc gacgttcctg actgacagct
      241 tagagctgga gctggggacg gaatggtgca aacctcctta cttttcttgt gctgttgaca
      301 acagaggagg aggaaaacat ttttctggag aatcctacct ctgcagcgga gcccttaagc
      361 gattgatttt gaatcttgac cctttaccaa ctaattttga agaagatacc ttggaaatat
      421 ttggcattca gtgggttact gaaacagcat tagtgaattc atctagagaa ctctttcatt
      481 tattcaggca acaactgtac aacttggaaa ccttgttaca gtccagttgt gattttggga
      541 aggtatcaac tctacactgc aaagcagaca atattaggca gcagtgtgta ctatttctcc
      601 attatgttaa agttttcatc ttcaggtatc tgaaagtaca gaatgctgag agtcatgttc
      661 ctgtccatcc ttatgaggct ttggaggctc agcttccctc agtgttgatt gatgagcttc
      721 atggattact cttgtatatt ggacacctat ctgaacttcc cagtgttaat ataggagcat
      781 ttgtaaatca aaaccagatt aagctttttc caccgtcatg gcatttatta catctccact
      841 tggatataca ttggctggtg ctagaaattc tttacatgct gggtgaaaaa ttgaaacaag
      901 ttgtatatgg tcatcagttt atgaatctgg caagtgacaa tttaaccaac atcagcctat
      961 ttgaagaaca ttgtgaaact ctcctttgtg atttaataag cctgtcactc aacaggtacg
     1021 acaaggttag gtcttctgaa tcattaatga gtgaccagtg tccatgttta tgcattaaag
     1081 aattatgggt tctacttatt catcttctag accacagaag taaatggttt gtctcggaat
     1141 cattttggaa ctggttgaat aaactactta aaacactgct tgaaaaatca agtgaccgaa
     1201 gaagatcctc tatgcctgta atccagtcca gggatccatt aggttttagt tggtggatta
     1261 ttactcatgt agcatcattt tacaagtttg atcgccatgg agtaccagat gaaatgagaa
     1321 aagtggaatc aaattggaac tttgtagaag aactgctgaa aaagtccatc agtgttcagg
     1381 gtgtcattct agaagaacaa ttacgaatgt atcttcactg ttgtttgaca ctttgtgatt
     1441 tctgggagcc aaacattgca attgttacca ttttatggga atattatagt aagaacctga
     1501 atagttcctt cagtatttct tggcttcctt ttaaaggcct tgctaatacc atgaagtcac
     1561 ccttgtctat gcttgaaatg gtgaagactt gctgttgcga taaacaagat caggaactat
     1621 ataaatccag cagtagttat actatttttc tttgtattct ggcaaaagtt gttaaaaaag
     1681 caatgaagag caatggccct catccttgga aacaagtcaa aggaagaata tattcaaaat
     1741 tccatcaaaa aagaatggaa gaactaactg aagttggtct acagaacttt tttagccttt
     1801 ttctactgtt agcagctgtt gcagaggtag aagatgttgc aagtcatgtt ttagacctcc
     1861 tgaatttcct caagcctgct tttgtaacgt ctcagagagc cctcatttgg aagggtcaca
     1921 tggccttcct cttgatgtat gcccagaaaa atctggacat tggtgttttg gctgagaaat
     1981 tttcatgtgc tttccgggag aaagcaaagg aattcttggt gtctaagaat gaggaaatgg
     2041 tacagagaca gactatctgg acccttcttt ccatatacat tgatggtgtt caagaagtgt
     2101 ttgagaccag ctattgcttg tatccttccc atgaaaaact gcttaatgat ggatttagta
     2161 tgcttctgcg agcatgtcga gaatctgaac ttaggacagt attgagcttc ctacaagctg
     2221 ttctggccag aatcaggagt atgcatcaac aattgtgtca ggaacttcaa agggacaatg
     2281 tggacctatt tgtacagtct tcattatcgg ctaaagagcg ccaccttgct gcagttgcca
     2341 gtgcactgtg gagacatttc ttttcatttt tgaagagtca gagaatgtca caggtagtgc
     2401 ctttctcaca acttgcggat gcagctgcag actttacttt gctagcaatg gacatgccaa
     2461 gcacagctcc atcagatttt cagcctcagc cagttatatc aattattcaa ctttttggtt
     2521 gggatgatat catctgccct caagttgtag caagatattt aagtcatgtc ctacaaaata
     2581 gcacattatg tgaagcactt tctcattcag gctatgtatc ttttcaagcc ttaaccgtaa
     2641 gatcatggat tcgttgtgtt ttgcaaatgt atattaaaaa cctctctggg cctgatgatt
     2701 tgctcataga taaaaatctg gaagaggcag ttgaaaaaga gtacatgaaa cagttggtca
     2761 aactgacaag attactattt aatctctcag aagtaaagag tattttctca aaggcccaag
     2821 ttgaatattt atccatctca gaagacccta aaaaagcact tgttcgattc tttgaggctg
     2881 ttggtgtaac ttacgggaac gtccagacac tttctgataa atctgccatg gtcacaaagt
     2941 ccttggaata ccttggtgaa gtattaaaat atattaagcc ttatttggga aaaaaagttt
     3001 tcagtgcagg gctgcagctg acttatggaa tgatgggaat tcttgtgaaa tcatgggcac
     3061 aaatctttgc cacttctaaa gcccaaaaat tactattccg gatcatagat tgtttactgc
     3121 tgccacatgc agtattacag caagagaagg aactgcctgc acctatgttg tcagcaattc
     3181 agaaaagtct tcctttgtat ctccagggca tgtgtatcgt gtgttgtcaa tctcaaaatc
     3241 cgaatgccta tttgaatcaa ttgctaggga atgttattga gcagtatatt gggcgatttc
     3301 ttccagcttc accatatgtt tcagatcttg gacaacatcc tgttttgctg gcattgagaa
     3361 acacagccac tattccacca atatcatctc taaagaaatg cattgtgcaa gtcataagga
     3421 aatcctacct tgagtataag gggtcctcac ctcctcctcg cttagcatcc attctggcct
     3481 tcatcctcca actcttcaag gaaactaaca cagacattta tgaagttgaa ctactcctcc
     3541 ctggcatttt aaaatgcttg gtgttagtca gtgaaccaca agttaaaagg ctggccacag
     3601 agaacctgca atacatggta aaagcctgcc aagtggggtc agaagaagaa ccttcctccc
     3661 agctgacttc tgtgtttagg cagtttatcc aggattatgg tatgaggtac tattaccagg
     3721 tttacagcat tttagaaaca gtagcaacat tggaccagca ggttgtcatc cacttgattt
     3781 ctacccttac tcagtctctg aaggattcag agcagaaatg gggccttggc aggaatatag
     3841 cacaaaggga agcctatagc aaacttttgt ctcaccttgg acagatggga caagatgaga
     3901 tgcagagact ggaaaatgat aatacttaat atattttgtg attcatcctg tctattaatt
     3961 atcagtatct aaagccacat tgttccactc taattcctac ttttaattaa ttgtataatg
     4021 ttctgtttaa aataatttct aggatttttt ttccttttat atctgtaagt aaaaaatgta
     4081 taaataagga actgaaggtt aattagcaac tgtgtaatag gatccttgga actttttaca
     4141 aaactgcatt ctaccttgat gaaattttag ttaaactaaa ttaaagaaac attttctgag
     4201 agtgttttct gtgtgtgagt attaagcaga tacttcatcc atagactttt tgttgtagta
     4261 tgacttcctg cctccctgcc ttgtgactga tttgtcatgt ttatttttta tttttacaat
     4321 aatagttatg ttttaaaatg atactttttc aaagtacctg gctaccctat actgtgtttt
     4381 tggtggggga aaggtgttgg gaaatgtgac tgtgtatttg tttttctttt ttatatgtgt
     4441 gaggcaagtt aacaaagaaa acccttacaa agcacactta actgtgttag gaatgagact
     4501 gaatctggct aaaggattat acagctgata tccaaaaaga agtgtaaaca tgccaacagg
     4561 gtttatattt aggttccaag agttgccaaa tttttttttt attcatcctt tcttttggag
     4621 ttgtcattaa aaaaaaaatc atatcaggag gaggaaataa aggagacttt aagaggagag
     4681 taaataaaaa tattctagaa aagtaacaat agtagtggtc tgagaagtca gctgatccat
     4741 cttcttgctc tttagccaga gcttcttata acttagcatt ttatccaaga aacctgttta
     4801 gtttccttta acaacattgt ttctcaactt ttgaactctt aactcatcca gattgtttta
     4861 ccagttggag cttgtccttg actccttttc ctattgagca ttatttggtt gaaagagctc
     4921 cagtcctagt tttgggaacc cacctgggtc tcaaactcag ctttgcttta tgatcttgag
     4981 ctagttacgt aacctttctc agtctagttt cttcatggag aagaaaagaa gaatcccttt
     5041 actatttgac agtgttgttg tgagaatcaa tgggataatg tacgtgaaaa tatttggtgg
     5101 cccaaattct gttgggaaat gtacagacac gattagttta ttactcgtgc acaattaagg
     5161 caaagaacac ctctcaattt tatctttctc taatgatatg tcccagatga atgatcccac
     5221 tttccaatgt tttcttacat catgttttct aactttgaag tactttgttt ccttcttggg
     5281 attatagtct ttatcatggt tctactcttc cttcaagaaa acatttcaac ccttctctgg
     5341 ctcaaggagt atctttaaaa atgaaatact ttgcaatcag tattctgtag ttaatagcgt
     5401 tctcttaatt ataatctgtg aaatccaaag gagtgtctgt tattttcata acatataaat
     5461 ggtccttctc tcaaatcagc ttcatcaaag ggaataaatg aaagcttaag gctgtttaat
     5521 agacttcata acactgaatt tcttttcatc atgatagtga cttactaaac aaataatgta
     5581 tggcttggaa agttccagca gtcactaagg agttttatct atttaaggtg atgcctttaa
     5641 agaggttatt tatgtgtata tgtgtttttg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     5701 tgtgtgtgtg ggtttttttt ttccaaagga gattgataaa gatacctttg agaatcttgc
     5761 tccttagagt atgaaaacgt ttaaaggata ggaaaagctg ctactgaatc atgctttagg
     5821 acatatatct atagatacta agaataacta aaatttgtac ttaagaatgg taacaacaaa
     5881 gtgtgtaaca atatgtgtgt gtattatatg ttttgtattt atgtataagt aacttttttt
     5941 aaagcttttt tttaaattga aaaaagaaga aaatttatgg ctcagttcct tttttttaaa
     6001 atcttcactg atccttcact tattactaca atcttgccct ctttcattcc cagacttgga
     6061 gtcccttata cctgcttctt taatagccac tttccctaaa taccctgtaa tttctgaggc
     6121 tgctgcttct accagtgtaa tacaagccat tgtcttttgg tcaaatctga gtttgttctc
     6181 ctttctcatg ttctttgtcc tctctgtgac aattgtattt tcttttatct tcccatcatt
     6241 tccacaatgg tactgctcct tcctacatat acagttgacc cttgagcaac acaagtttga
     6301 actgtgcagg tccacttata tgtggatcta aaatacagta tttgtgggat gcaaaatctg
     6361 agtatatgga ggaccaactt tttgtagagg caggttcctc agggttaact gcaggacttg
     6421 agtaagtgca gattatggta tgcacagggg atcctggaac cagtccccag accaaaaatg
     6481 accactgtat cttgagagca ccttcatggt cttctttcct gtccccagtc gtaagatacg
     6541 acccttcatc tgctttgtct caacattctg ttagtggtga actgataact ccagcttttc
     6601 tctctctttt tttttttttt tttttttttg ttgttgttgt tgttgttgtt ttgaggcaga
     6661 gtcttgctct gtcgcccagg ctggagtgca gtggcacgat ctcggctcac tgcaagctct
     6721 gcctcccggg ttcacgccat tctcctgcct cagcctccca agtagctggg actacaggtg
     6781 cccgccacca cgcccggcta attttttttg tatttttagt agagacgggg tttcaccatg
     6841 ttgggctcga tctcctgacc tcatgatccg cccgcctcgg cctcccaaag tgctgggatt
     6901 acaggcgtga tgagccacct agtgctaatt catctctaaa cctctgtagt tggagtctcc
     6961 tgaaatctca gactacctcc tgctttcatt ttctacccag ggaatacact attgtcctaa
     7021 gttatttagc cttgaaatgt cagaggcatt ttaggttctt catgtttctc ccactttcag
     7081 ttgattacca ttacaggatc ttaggcttct ttttatcttt ttgcagtgcc ttgcatccac
     7141 tggttgtttg ttttttctgt tgggcactac ttatttcctt acctctggtt tcttctccta
     7201 cagcttatgt tctgaaatgt gtattcctaa aatactgttt tcctcttgtt acttttctgc
     7261 tcgaggactt ccagtaactt accattgttt ataaggtgat gttcaaaccc ttcagtctgg
     7321 agactttctg ccacctatct gactgtacct ttatcttcaa gcttatctct ttccaaacaa
     7381 gaggctcatt tagcagcttt ctctgattgc catgttcatc ctacactcag cctcattcag
     7441 tttacagcat ggaaactgta taggacctct ttcctataga aattgaagac acttaaatag
     7501 gaagaaaatt aaaatataca tttggataca tgagtattcc agtcaaataa tatctataaa
     7561 ataccagata gagtataaaa gacaactgaa ggacaacaga gtgatgaaag gactttatta
     7621 ggcatttgga tttggttatg atttaaattt caatttaatt agaacgtttc catggcaagg
     7681 aaggaagcat ggaggactgt ggaaaagtca ttcagtattg agttcatttg cattagagga
     7741 atttcatagt ttaaaacttg tatatcttta cctatccttc gtatgttttc ttcttaagca
     7801 tatttgactt tttctacctc agcatctgta taagaaaata tttgtgagtc agatgtttgt
     7861 gggttttcct tacctattat tattttcttc catgctttac aacacatttt ttaaactacc
     7921 ttgttcttaa ataattacac ggacctgctt ctgtgtactt tcacagaatc tttgacagtt
     7981 aaaaattgta tgttatataa aaatttgaca agcttctaca gttaggaaaa gcctttagaa
     8041 atctgccttc cccaaaccgt atgttatcat agcactcatg tctccccatg tctaaaaggt
     8101 aaatagatac agaaacctca ccaaaagttg aatcatctgt actaaaccac tgctttttta
     8161 tccagacttt ttatataaat cttttatttt ccaaaaaact gatcttttac ctacccctct
     8221 tagagttaaa aatattgcct gtcaggcaag agtatagtat gccaatataa ataatatgct
     8281 ttatttggtt agaaacagtt ggtttgggaa gctagcaaca tagcatattc tttacaaatt
     8341 taataaggtg tattttgata ctgtgataac ctgcattgta aattaccaaa tggttggaat
     8401 tgaccagttt atacttatta aaatttttga tgtgccaatg gctatccaat tcattgcatt
     8461 tgtgaattgt gcttttagaa aaattgtgga cattttagtt ttatatacat atttatgagt
     8521 tatttgtgca tatgataaaa ttatatataa taataaaagt atatatgtaa ctta
//
