LOCUS       NM_004994               2336 bp    mRNA    linear   PRI 15-MAR-2025
DEFINITION  Homo sapiens matrix metallopeptidase 9 (MMP9), mRNA.
ACCESSION   NM_004994
VERSION     NM_004994.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Khan,S., Khan,N.U., Khan,Y., Shehzad,I., Alanzi,A.R. and Chen,T.
  TITLE     Preliminary investigation of MMP8 (rs11225395) and MMP9 (rs3787268)
            polymorphisms association with breast cancer risk in pashtun women
            of Pakistan
  JOURNAL   Mol Biol Rep 51 (1), 1034 (2024)
   PUBMED   39361067
  REMARK    GeneRIF: Preliminary investigation of MMP8 (rs11225395) and MMP9
            (rs3787268) polymorphisms association with breast cancer risk in
            pashtun women of Pakistan.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Riguetto,C.M., Barbosa,E.B., Atihe,C.C., Reis,F., Alves,M. and
            Zantut-Wittmann,D.E.
  TITLE     Interaction of MMP-9 in the active phase of Graves' disease with
            and without ophthalmopathy
  JOURNAL   Am J Physiol Endocrinol Metab 327 (4), E577-E584 (2024)
   PUBMED   39259164
  REMARK    GeneRIF: Interaction of MMP-9 in the active phase of Graves'
            disease with and without ophthalmopathy.
REFERENCE   3  (bases 1 to 2336)
  AUTHORS   Xue,R., Fan,Z. and An,Y.
  TITLE     Knockdown of PRDX2 Inhibits the Proliferation, Growth, Migration,
            Invasion, and MMP9 Activity of Ewing's Sarcoma Cells Cultured In
            Vitro
  JOURNAL   Cancer Rep (Hoboken) 7 (9), e2122 (2024)
   PUBMED   39234629
  REMARK    GeneRIF: Knockdown of PRDX2 Inhibits the Proliferation, Growth,
            Migration, Invasion, and MMP9 Activity of Ewing's Sarcoma Cells
            Cultured In Vitro.
REFERENCE   4  (bases 1 to 2336)
  AUTHORS   Chiu,K.L., He,J.L., Chen,G.L., Shen,T.C., Chen,L.H., Chen,J.C.,
            Tsai,C.W., Chang,W.S., Hsia,T.C. and Bau,D.T.
  TITLE     Impacts of Matrix Metalloproteinase-9 Promoter Genotypes on Asthma
            Risk
  JOURNAL   In Vivo 38 (5), 2144-2151 (2024)
   PUBMED   39187355
  REMARK    GeneRIF: Impacts of Matrix Metalloproteinase-9 Promoter Genotypes
            on Asthma Risk.
REFERENCE   5  (bases 1 to 2336)
  AUTHORS   Ishibashi,R., Itani,M., Kawashima,A., Arakawa,Y. and Aoki,T.
  TITLE     JNK2-MMP-9 axis facilitates the progression of intracranial
            aneurysms
  JOURNAL   Sci Rep 14 (1), 19458 (2024)
   PUBMED   39169203
  REMARK    GeneRIF: JNK2-MMP-9 axis facilitates the progression of
            intracranial aneurysms.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2336)
  AUTHORS   Triebel,S., Blaser,J., Reinke,H. and Tschesche,H.
  TITLE     A 25 kDa alpha 2-microglobulin-related protein is a component of
            the 125 kDa form of human gelatinase
  JOURNAL   FEBS Lett 314 (3), 386-388 (1992)
   PUBMED   1281792
REFERENCE   7  (bases 1 to 2336)
  AUTHORS   Okada,Y., Gonoji,Y., Naka,K., Tomita,K., Nakanishi,I., Iwata,K.,
            Yamashita,K. and Hayakawa,T.
  TITLE     Matrix metalloproteinase 9 (92-kDa gelatinase/type IV collagenase)
            from HT 1080 human fibrosarcoma cells. Purification and activation
            of the precursor and enzymic properties
  JOURNAL   J Biol Chem 267 (30), 21712-21719 (1992)
   PUBMED   1400481
REFERENCE   8  (bases 1 to 2336)
  AUTHORS   Ogata,Y., Enghild,J.J. and Nagase,H.
  TITLE     Matrix metalloproteinase 3 (stromelysin) activates the precursor
            for the human matrix metalloproteinase 9
  JOURNAL   J Biol Chem 267 (6), 3581-3584 (1992)
   PUBMED   1371271
REFERENCE   9  (bases 1 to 2336)
  AUTHORS   Masure,S., Proost,P., Van Damme,J. and Opdenakker,G.
  TITLE     Purification and identification of 91-kDa neutrophil gelatinase.
            Release by the activating peptide interleukin-8
  JOURNAL   Eur J Biochem 198 (2), 391-398 (1991)
   PUBMED   1645657
REFERENCE   10 (bases 1 to 2336)
  AUTHORS   Van Ranst,M., Norga,K., Masure,S., Proost,P., Vandekerckhove,F.,
            Auwerx,J., Van Damme,J. and Opdenakker,G.
  TITLE     The cytokine-protease connection: identification of a 96-kD THP-1
            gelatinase and regulation by interleukin-1 and cytokine inducers
  JOURNAL   Cytokine 3 (3), 231-239 (1991)
   PUBMED   1653055
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from J05070.1, CN288579.1,
            AW468235.1 and BC006093.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004994.2.
            
            Summary: Proteins of the matrix metalloproteinase (MMP) family are
            involved in the breakdown of extracellular matrix in normal
            physiological processes, such as embryonic development,
            reproduction, and tissue remodeling, as well as in disease
            processes, such as arthritis and metastasis. Most MMP's are
            secreted as inactive proproteins which are activated when cleaved
            by extracellular proteinases. The enzyme encoded by this gene
            degrades type IV and V collagens. Studies in rhesus monkeys suggest
            that the enzyme is involved in IL-8-induced mobilization of
            hematopoietic progenitor cells from bone marrow, and murine studies
            suggest a role in tumor-associated tissue remodeling. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J05070.1, BC006093.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372330.3/ ENSP00000361405.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-854               J05070.1           1-854
            855-1406            CN288579.1         43-594
            1407-1739           J05070.1           1407-1739
            1740-1743           AW468235.1         612-615             c
            1744-2161           J05070.1           1744-2161
            2162-2336           BC006093.1         2148-2322
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..2336
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /note="matrix metallopeptidase 9"
                     /db_xref="GeneID:4318"
                     /db_xref="HGNC:HGNC:7176"
                     /db_xref="MIM:120361"
     exon            1..157
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..2143
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /EC_number="3.4.24.35"
                     /note="type V collagenase; matrix metalloproteinase 9
                     (gelatinase B, 92kDa gelatinase, 92kDa type IV
                     collagenase); macrophage gelatinase; matrix
                     metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa
                     type IV collagenase)"
                     /codon_start=1
                     /product="matrix metalloproteinase-9 preproprotein"
                     /protein_id="NP_004985.2"
                     /db_xref="CCDS:CCDS13390.1"
                     /db_xref="GeneID:4318"
                     /db_xref="HGNC:HGNC:7176"
                     /db_xref="MIM:120361"
                     /translation="MSLWQPLVLVLLVLGCCFAAPRQRQSTLVLFPGDLRTNLTDRQL
                     AEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCGVP
                     DLGRFQTFEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVY
                     SRDADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGVVV
                     PTRFGNADGAACHFPFIFEGRSYSACTTDGRSDGLPWCSTTANYDTDDRFGFCPSERL
                     YTQDGNADGKPCQFPFIFQGQSYSACTTDGRSDGYRWCATTANYDRDKLFGFCPTRAD
                     STVMGGNSAGELCVFPFTFLGKEYSTCTSEGRGDGRLWCATTSNFDSDKKWGFCPDQG
                     YSLFLVAAHEFGHALGLDHSSVPEALMYPMYRFTEGPPLHKDDVNGIRHLYGPRPEPE
                     PRPPTTTTPQPTAPPTVCPTGPPTVHPSERPTAGPTGPPSAGPTGPPTAGPSTATTVP
                     LSPVDDACNVNIFDAIAEIGNQLYLFKDGKYWRFSEGRGSRPQGPFLIADKWPALPRK
                     LDSVFEERLSKKLFFFSGRQVWVYTGASVLGPRRLDKLGLGADVAQVTGALRSGRGKM
                     LLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRV
                     SSRSELNQVDQVGYVTYDILQCPED"
     exon            158..390
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            391..539
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            540..668
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            669..842
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            843..1016
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1193
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1349
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1629
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..1769
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1920
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..2024
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2336
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2311..2316
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /note="hexamer: AATAAA"
     polyA_site      2336
                     /gene="MMP9"
                     /gene_synonym="CLG4B; GELB; MANDP2; MMP-9"
                     /note="major polyA site"
ORIGIN      
        1 agacacctct gccctcacca tgagcctctg gcagcccctg gtcctggtgc tcctggtgct
       61 gggctgctgc tttgctgccc ccagacagcg ccagtccacc cttgtgctct tccctggaga
      121 cctgagaacc aatctcaccg acaggcagct ggcagaggaa tacctgtacc gctatggtta
      181 cactcgggtg gcagagatgc gtggagagtc gaaatctctg gggcctgcgc tgctgcttct
      241 ccagaagcaa ctgtccctgc ccgagaccgg tgagctggat agcgccacgc tgaaggccat
      301 gcgaacccca cggtgcgggg tcccagacct gggcagattc caaacctttg agggcgacct
      361 caagtggcac caccacaaca tcacctattg gatccaaaac tactcggaag acttgccgcg
      421 ggcggtgatt gacgacgcct ttgcccgcgc cttcgcactg tggagcgcgg tgacgccgct
      481 caccttcact cgcgtgtaca gccgggacgc agacatcgtc atccagtttg gtgtcgcgga
      541 gcacggagac gggtatccct tcgacgggaa ggacgggctc ctggcacacg cctttcctcc
      601 tggccccggc attcagggag acgcccattt cgacgatgac gagttgtggt ccctgggcaa
      661 gggcgtcgtg gttccaactc ggtttggaaa cgcagatggc gcggcctgcc acttcccctt
      721 catcttcgag ggccgctcct actctgcctg caccaccgac ggtcgctccg acggcttgcc
      781 ctggtgcagt accacggcca actacgacac cgacgaccgg tttggcttct gccccagcga
      841 gagactctac acccaggacg gcaatgctga tgggaaaccc tgccagtttc cattcatctt
      901 ccaaggccaa tcctactccg cctgcaccac ggacggtcgc tccgacggct accgctggtg
      961 cgccaccacc gccaactacg accgggacaa gctcttcggc ttctgcccga cccgagctga
     1021 ctcgacggtg atggggggca actcggcggg ggagctgtgc gtcttcccct tcactttcct
     1081 gggtaaggag tactcgacct gtaccagcga gggccgcgga gatgggcgcc tctggtgcgc
     1141 taccacctcg aactttgaca gcgacaagaa gtggggcttc tgcccggacc aaggatacag
     1201 tttgttcctc gtggcggcgc atgagttcgg ccacgcgctg ggcttagatc attcctcagt
     1261 gccggaggcg ctcatgtacc ctatgtaccg cttcactgag gggcccccct tgcataagga
     1321 cgacgtgaat ggcatccggc acctctatgg tcctcgccct gaacctgagc cacggcctcc
     1381 aaccaccacc acaccgcagc ccacggctcc cccgacggtc tgccccaccg gaccccccac
     1441 tgtccacccc tcagagcgcc ccacagctgg ccccacaggt cccccctcag ctggccccac
     1501 aggtcccccc actgctggcc cttctacggc cactactgtg cctttgagtc cggtggacga
     1561 tgcctgcaac gtgaacatct tcgacgccat cgcggagatt gggaaccagc tgtatttgtt
     1621 caaggatggg aagtactggc gattctctga gggcaggggg agccggccgc agggcccctt
     1681 ccttatcgcc gacaagtggc ccgcgctgcc ccgcaagctg gactcggtct ttgaggagcg
     1741 gctctccaag aagcttttct tcttctctgg gcgccaggtg tgggtgtaca caggcgcgtc
     1801 ggtgctgggc ccgaggcgtc tggacaagct gggcctggga gccgacgtgg cccaggtgac
     1861 cggggccctc cggagtggca gggggaagat gctgctgttc agcgggcggc gcctctggag
     1921 gttcgacgtg aaggcgcaga tggtggatcc ccggagcgcc agcgaggtgg accggatgtt
     1981 ccccggggtg cctttggaca cgcacgacgt cttccagtac cgagagaaag cctatttctg
     2041 ccaggaccgc ttctactggc gcgtgagttc ccggagtgag ttgaaccagg tggaccaagt
     2101 gggctacgtg acctatgaca tcctgcagtg ccctgaggac tagggctccc gtcctgcttt
     2161 ggcagtgcca tgtaaatccc cactgggacc aaccctgggg aaggagccag tttgccggat
     2221 acaaactggt attctgttct ggaggaaagg gaggagtgga ggtgggctgg gccctctctt
     2281 ctcacctttg ttttttgttg gagtgtttct aataaacttg gattctctaa ccttta
//
