LOCUS       NM_002424               3055 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens matrix metallopeptidase 8 (MMP8), transcript variant
            1, mRNA.
ACCESSION   NM_002424
VERSION     NM_002424.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Khan,S., Khan,N.U., Khan,Y., Shehzad,I., Alanzi,A.R. and Chen,T.
  TITLE     Preliminary investigation of MMP8 (rs11225395) and MMP9 (rs3787268)
            polymorphisms association with breast cancer risk in pashtun women
            of Pakistan
  JOURNAL   Mol Biol Rep 51 (1), 1034 (2024)
   PUBMED   39361067
  REMARK    GeneRIF: Preliminary investigation of MMP8 (rs11225395) and MMP9
            (rs3787268) polymorphisms association with breast cancer risk in
            pashtun women of Pakistan.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Chen,C.H., Chin,Y.T., Hsu,S.W., Shih,L.C., Tien,H.C., Tsai,C.W.,
            Wang,Y.C., Liu,Y.F., Bau,D.T. and Chang,W.S.
  TITLE     Impact of Matrix Metalloproteinase-8 Polymorphisms on
            Nasopharyngeal Carcinoma Risk
  JOURNAL   Anticancer Res 44 (9), 3813-3820 (2024)
   PUBMED   39197904
  REMARK    GeneRIF: Impact of Matrix Metalloproteinase-8 Polymorphisms on
            Nasopharyngeal Carcinoma Risk.
REFERENCE   3  (bases 1 to 3055)
  AUTHORS   Wu,Y., Liu,H., Sun,Z., Liu,J., Li,K., Fan,R., Dai,F., Tang,H.,
            Hou,Q., Li,J. and Tang,X.
  TITLE     The adhesion-GPCR ADGRF5 fuels breast cancer progression by
            suppressing the MMP8-mediated antitumorigenic effects
  JOURNAL   Cell Death Dis 15 (6), 455 (2024)
   PUBMED   38937435
  REMARK    GeneRIF: The adhesion-GPCR ADGRF5 fuels breast cancer progression
            by suppressing the MMP8-mediated antitumorigenic effects.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3055)
  AUTHORS   Tavakoli,F., Faramarzi,M., Salimnezhad,S., Jafari,B., Eslami,H. and
            MohammadPourTabrizi,B.
  TITLE     Comparing the activity level of salivary matrix metalloproteinase-8
            in patients with diabetes and moderate to severe chronic
            generalized periodontitis
  JOURNAL   Clin Exp Dent Res 10 (2), e865 (2024)
   PUBMED   38433295
  REMARK    GeneRIF: Comparing the activity level of salivary matrix
            metalloproteinase-8 in patients with diabetes and moderate to
            severe chronic generalized periodontitis.
REFERENCE   5  (bases 1 to 3055)
  AUTHORS   Wang,T.Y., Weng,E.F., Hsu,Y.C., Shiu,L.P., Huang,T.W., Wu,H.C.,
            Hong,J.S. and Wang,S.M.
  TITLE     Inhibition of MMP8 effectively alleviates manic-like behavior and
            reduces neuroinflammation by modulating astrocytic CEBPD
  JOURNAL   J Neuroinflammation 21 (1), 61 (2024)
   PUBMED   38419037
  REMARK    GeneRIF: Inhibition of MMP8 effectively alleviates manic-like
            behavior and reduces neuroinflammation by modulating astrocytic
            CEBPD.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3055)
  AUTHORS   Farr,M., Pieper,M., Calvete,J. and Tschesche,H.
  TITLE     The N-terminus of collagenase MMP-8 determines superactivity and
            inhibition: a relation of structure and function analyzed by
            biomolecular interaction analysis
  JOURNAL   Biochemistry 38 (22), 7332-7338 (1999)
   PUBMED   10353844
REFERENCE   7  (bases 1 to 3055)
  AUTHORS   Blaser,J., Triebel,S., Reinke,H. and Tschesche,H.
  TITLE     Formation of a covalent Hg-Cys-bond during mercurial activation of
            PMNL procollagenase gives evidence of a cysteine-switch mechanism
  JOURNAL   FEBS Lett 313 (1), 59-61 (1992)
   PUBMED   1330697
REFERENCE   8  (bases 1 to 3055)
  AUTHORS   Blaser,J., Knauper,V., Osthues,A., Reinke,H. and Tschesche,H.
  TITLE     Mercurial activation of human polymorphonuclear leucocyte
            procollagenase
  JOURNAL   Eur J Biochem 202 (3), 1223-1230 (1991)
   PUBMED   1662606
REFERENCE   9  (bases 1 to 3055)
  AUTHORS   Devarajan,P., Mookhtiar,K., Van Wart,H. and Berliner,N.
  TITLE     Structure and expression of the cDNA encoding human neutrophil
            collagenase
  JOURNAL   Blood 77 (12), 2731-2738 (1991)
   PUBMED   1646048
REFERENCE   10 (bases 1 to 3055)
  AUTHORS   Knauper,V., Kramer,S., Reinke,H. and Tschesche,H.
  TITLE     Characterization and activation of procollagenase from human
            polymorphonuclear leucocytes. N-terminal sequence determination of
            the proenzyme and various proteolytically activated forms
  JOURNAL   Eur J Biochem 189 (2), 295-300 (1990)
   PUBMED   2159879
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DW009698.1, BC074988.2 and
            AP000647.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002424.2.
            
            Summary: This gene encodes a member of the matrix metalloproteinase
            (MMP) family of proteins. These proteins are involved in the
            breakdown of extracellular matrix in embryonic development,
            reproduction, and tissue remodeling, as well as in disease
            processes, such as arthritis and metastasis. Proteolysis at
            different sites on this protein results in multiple active forms of
            the enzyme with distinct N-termini. This protein functions in the
            degradation of type I, II and III collagens. The gene is part of a
            cluster of MMP genes which localize to chromosome 11q22.3.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (1) represents the shortest
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292659.1, AK313374.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000236826.8/ ENSP00000236826.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-61                DW009698.1         353-413             c
            62-1524             BC074988.2         1-1463
            1525-3055           AP000647.5         12546-14076         c
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q22.2"
     gene            1..3055
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /note="matrix metallopeptidase 8"
                     /db_xref="GeneID:4317"
                     /db_xref="HGNC:HGNC:7175"
                     /db_xref="MIM:120355"
     exon            1..201
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..1503
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /EC_number="3.4.24.34"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; PMNL collagenase; matrix metalloproteinase 8
                     (neutrophil collagenase); matrix metalloproteinase-8;
                     collagenase 2; PMN leukocyte collagenase"
                     /codon_start=1
                     /product="neutrophil collagenase isoform 1 preproprotein"
                     /protein_id="NP_002415.1"
                     /db_xref="CCDS:CCDS8320.1"
                     /db_xref="GeneID:4317"
                     /db_xref="HGNC:HGNC:7175"
                     /db_xref="MIM:120355"
                     /translation="MFSLKTLPFLLLLHVQISKAFPVSSKEKNTKTVQDYLEKFYQLP
                     SNQYQSTRKNGTNVIVEKLKEMQRFFGLNVTGKPNEETLDMMKKPRCGVPDSGGFMLT
                     PGNPKWERTNLTYRIRNYTPQLSEAEVERAIKDAFELWSVASPLIFTRISQGEADINI
                     AFYQRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDAEETWTNTSANYNLFLVAAHE
                     FGHSLGLAHSSDPGALMYPNYAFRETSNYSLPQDDIDGIQAIYGLSSNPIQPTGPSTP
                     KPCDPSLTFDAITTLRGEILFFKDRYFWRRHPQLQRVEMNFISLFWPSLPTGIQAAYE
                     DFDRDLIFLFKGNQYWALSGYDILQGYPKDISNYGFPSSVQAIDAAVFYRSKTYFFVN
                     DQFWRYDNQRQFMEPGYPKSISGAFPGIESKVDAVFQQEHFFHVFSGPRYYAFDLIAQ
                     RVTRVARGNKWLNCRYG"
     exon            202..446
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     exon            447..595
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     exon            596..721
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     exon            722..883
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1001
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1135
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1289
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1393
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..3055
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2384..2389
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /note="hexamer: AATAAA"
     polyA_site      2408
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
     regulatory      2889..2894
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /note="hexamer: AATAAA"
     polyA_site      2914
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /note="major polyA site"
     regulatory      3032..3037
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
                     /note="hexamer: ATTAAA"
     polyA_site      3055
                     /gene="MMP8"
                     /gene_synonym="CLG1; HNC; MMP-8; PMNL-CL"
ORIGIN      
        1 gacacatgat gctgtgaacg tcagggtgct cgccagggaa gggccctacc cagagggaca
       61 gaaagaaagc caggaggggt agagtttgaa gagaagatca tgttctccct gaagacgctt
      121 ccatttctgc tcttactcca tgtgcagatt tccaaggcct ttcctgtatc ttctaaagag
      181 aaaaatacaa aaactgttca ggactacctg gaaaagttct accaattacc aagcaaccag
      241 tatcagtcta caaggaagaa tggcactaat gtgatcgttg aaaagcttaa agaaatgcag
      301 cgattttttg ggttgaatgt gacggggaag ccaaatgagg aaactctgga catgatgaaa
      361 aagcctcgct gtggagtgcc tgacagtggt ggttttatgt taaccccagg aaaccccaag
      421 tgggaacgca ctaacttgac ctacaggatt cgaaactata ccccacagct gtcagaggct
      481 gaggtagaaa gagctatcaa ggatgccttt gaactctgga gtgttgcatc acctctcatc
      541 ttcaccagga tctcacaggg agaggcagat atcaacattg ctttttacca aagagatcac
      601 ggtgacaatt ctccatttga tggacccaat ggaatccttg ctcatgcctt tcagccaggc
      661 caaggtattg gaggagatgc tcattttgat gccgaagaaa catggaccaa cacctccgca
      721 aattacaact tgtttcttgt tgctgctcat gaatttggcc attctttggg gctcgctcac
      781 tcctctgacc ctggtgcctt gatgtatccc aactatgctt tcagggaaac cagcaactac
      841 tcactccctc aagatgacat cgatggcatt caggccatct atggactttc aagcaaccct
      901 atccaaccta ctggaccaag cacacccaaa ccctgtgacc ccagtttgac atttgatgct
      961 atcaccacac tccgtggaga aatacttttc tttaaagaca ggtacttctg gagaaggcat
     1021 cctcagctac aaagagtcga aatgaatttt atttctctat tctggccatc ccttccaact
     1081 ggtatacagg ctgcttatga agattttgac agagacctca ttttcctatt taaaggcaac
     1141 caatactggg ctctgagtgg ctatgatatt ctgcaaggtt atcccaagga tatatcaaac
     1201 tatggcttcc ccagcagcgt ccaagcaatt gacgcagctg ttttctacag aagtaaaaca
     1261 tacttctttg taaatgacca attctggaga tatgataacc aaagacaatt catggagcca
     1321 ggttatccca aaagcatatc aggtgccttt ccaggaatag agagtaaagt tgatgcagtt
     1381 ttccagcaag aacatttctt ccatgtcttc agtggaccaa gatattacgc atttgatctt
     1441 attgctcaga gagttaccag agttgcaaga ggcaataaat ggcttaactg tagatatggc
     1501 tgaagcaaaa tcaaatgtgg ctgtatccac tttcagaatg ttgaagggaa gttcagcaag
     1561 cattttcgtt acattgtgtc ctgcttatac ttttctcaat attaagtcat tgtttcccat
     1621 cactgtatcc attctacctg tcctccgtga aaatatgttt ggaatattcc actatttgca
     1681 gaggcttatt cagttcttac acattccatc ttacattagt gattccatca aagagaagga
     1741 aagtaagcct ttttgtcacc tcaatattta ctatttcaat acttacatat ctgacttcta
     1801 ggatttattg ttatattact tgcctatctg acttcataca tccctcagtt tcttaaaatg
     1861 tcctatgtat atcttctaca tgcaatttag aactagattt tggttagaag taaggattat
     1921 aaacaaccta gacagtaccc ttggccttta cagaaaatat ggtgctgttt tctacccttg
     1981 gaaagaaatg tagatgatat gtttcgtggg ttgaattgtg tcccccataa aagatatgtt
     2041 gaagttctaa ccccaggtac ccatgaatgt gagcttacca gggtctttgc agatgtaatt
     2101 agttaagtta aggtgagatc acactgaatt agggtgggct ctaaatccat tatgactgtt
     2161 gttcttataa gaagaagaga ggcatagtca cctaggggag gaggccgtat gaagacagag
     2221 gcagagattg gagtgacgca tctccaagcc aaggaattcc aaggactgta agccaccagt
     2281 agaagctttg aagaggcaag gaaggattcc ctccaatagc cttcaagtgt gaccctgctg
     2341 acacctgcag aattcggact tctatcctcc aaaaccgtga gggaataaat ttcctttgtt
     2401 ttaagccacc aactttgcaa tactttgtta cagcaaccct agacatgagg tactagacac
     2461 agtacatcta cacatatgaa aatgaatcaa cacagaatgc agaagtagaa cccttgctaa
     2521 ggactactgg gcatcttccc aggacagcag ccaaaagaga accaccactt cctctcctgc
     2581 ctcctccttg ctctctccta gagtccaaac ccaaatgggc cagttggatc tgatgttcgt
     2641 cagttcttta cttctatttc ctggggtact caggagggca cacactatag ataacttggg
     2701 ttagctgcat aaaattcaat gtctcattaa gttgcattaa actgagctta gatgtgtaag
     2761 tttgctaacg gatgggtttt tttgttaaga actataggat ttatgggacc aagtctagcg
     2821 agtccagata tcaaaatcat tataatgtta tatttgctgt tattagaata taatatagct
     2881 tattatacaa taaatatgta gactgtaaaa tatatttctc actagtacct cctattttct
     2941 ttctctgttg aagtttttaa atcccacaga taattaaatt ggcaccttta tgcttgttca
     3001 aaaattaaaa taatctatta aataagttca aattaaagat ttttacttca aatga
//
