LOCUS       NM_007289               5656 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens membrane metalloendopeptidase (MME), transcript
            variant 2b, mRNA.
ACCESSION   NM_007289
VERSION     NM_007289.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Zhang,B., Gang,Q., Meng,L., Li,Z., Chu,X., Wu,H., Yang,J., Huang,B.
            and Du,K.
  TITLE     A novel variant of biallelic MME gene associated with autosomal
            recessive late-onset distal hereditary motor neuropathy in Chinese
            families
  JOURNAL   BMC Med Genomics 17 (1), 223 (2024)
   PUBMED   39232784
  REMARK    GeneRIF: A novel variant of biallelic MME gene associated with
            autosomal recessive late-onset distal hereditary motor neuropathy
            in Chinese families.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Grosz,B.R., Parmar,J.M., Ellis,M., Bryen,S., Simons,C.,
            Reis,A.L.M., Stevanovski,I., Deveson,I.W., Nicholson,G., Laing,N.,
            Wallis,M., Ravenscroft,G., Kumar,K.R., Vucic,S. and Kennerson,M.L.
  TITLE     A deep intronic variant in MME causes autosomal recessive
            Charcot-Marie-Tooth neuropathy through aberrant splicing
  JOURNAL   J Peripher Nerv Syst 29 (2), 262-274 (2024)
   PUBMED   38860315
  REMARK    GeneRIF: A deep intronic variant in MME causes autosomal recessive
            Charcot-Marie-Tooth neuropathy through aberrant splicing.
REFERENCE   3  (bases 1 to 5656)
  AUTHORS   Shigematsu,K., Komori,N., Ideno,M. and Yamagishi,H.
  TITLE     'Evaluation of neprilysin activity in Adipose-Derived stem cells
            from Alzheimer's disease patients'
  JOURNAL   Neurosci Lett 825, 137705 (2024)
   PUBMED   38428725
  REMARK    GeneRIF: 'Evaluation of neprilysin activity in Adipose-Derived stem
            cells from Alzheimer's disease patients'.
REFERENCE   4  (bases 1 to 5656)
  AUTHORS   Chen,H., Huang,Z., Lei,A., Yu,X., Shen,M. and Wu,D.
  TITLE     miRNA-211-5p inhibition enhances the protective effect of
            hucMSC-derived exosome in Abeta1-40 -induced SH-SY5Y cells by
            increasing NEP expression
  JOURNAL   J Biochem Mol Toxicol 38 (1), e23624 (2024)
   PUBMED   38229323
  REMARK    GeneRIF: miRNA-211-5p inhibition enhances the protective effect of
            hucMSC-derived exosome in Abeta1-40 -induced SH-SY5Y cells by
            increasing NEP expression.
REFERENCE   5  (bases 1 to 5656)
  AUTHORS   Bird,T.D.
  TITLE     Charcot-Marie-Tooth Neuropathy Type 2 - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301462
REFERENCE   6  (bases 1 to 5656)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
REFERENCE   7  (bases 1 to 5656)
  AUTHORS   Shipp,M.A., Tarr,G.E., Chen,C.Y., Switzer,S.N., Hersh,L.B.,
            Stein,H., Sunday,M.E. and Reinherz,E.L.
  TITLE     CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides
            and regulates the growth of small cell carcinomas of the lung
  JOURNAL   Proc Natl Acad Sci U S A 88 (23), 10662-10666 (1991)
   PUBMED   1660144
REFERENCE   8  (bases 1 to 5656)
  AUTHORS   Vijayaraghavan,J., Scicli,A.G., Carretero,O.A., Slaughter,C.,
            Moomaw,C. and Hersh,L.B.
  TITLE     The hydrolysis of endothelins by neutral endopeptidase 24.11
            (enkephalinase)
  JOURNAL   J Biol Chem 265 (24), 14150-14155 (1990)
   PUBMED   2201681
REFERENCE   9  (bases 1 to 5656)
  AUTHORS   Barker,P.E., Shipp,M.A., D'Adamio,L., Masteller,E.L. and
            Reinherz,E.L.
  TITLE     The common acute lymphoblastic leukemia antigen gene maps to
            chromosomal region 3 (q21-q27)
  JOURNAL   J Immunol 142 (1), 283-287 (1989)
   PUBMED   2521237
REFERENCE   10 (bases 1 to 5656)
  AUTHORS   Skidgel,R.A.
  TITLE     Characterization of the metabolism of substance P and neurotensin
            by human angiotensin I converting enzyme and 'enkephalinase'
  JOURNAL   Prog Clin Biol Res 192, 371-378 (1985)
   PUBMED   2417254
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC117384.5 and AC106724.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 1, 2019 this sequence version replaced NM_007289.3.
            
            Summary: The protein encoded by this gene is a type II
            transmembrane glycoprotein and a common acute lymphocytic leukemia
            antigen that is an important cell surface marker in the diagnosis
            of human acute lymphocytic leukemia (ALL). The encoded protein is
            present on leukemic cells of pre-B phenotype, which represent 85%
            of cases of ALL. This protein is not restricted to leukemic cells,
            however, and is found on a variety of normal tissues. The protein
            is a neutral endopeptidase that cleaves peptides at the amino side
            of hydrophobic residues and inactivates several peptide hormones
            including glucagon, enkephalins, substance P, neurotensin,
            oxytocin, and bradykinin. [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (2b) is the longest transcript and
            includes alternate exon 2b. Variants 1, 1bis, 2a, 2b, 3, and 4 all
            encode the same isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.2490519.1,
                                           SRR14038192.2768796.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360490.7/ ENSP00000353679.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               AC117384.5         70927-71074         c
            149-318             AC117384.5         67066-67235         c
            319-354             AC117384.5         66299-66334         c
            355-516             AC117384.5         36238-36399         c
            517-597             AC117384.5         34834-34914         c
            598-693             AC117384.5         34634-34729         c
            694-812             AC117384.5         34407-34525         c
            813-878             AC117384.5         32582-32647         c
            879-1013            AC117384.5         13157-13291         c
            1014-1115           AC117384.5         11101-11202         c
            1116-1252           AC117384.5         9266-9402           c
            1253-1346           AC117384.5         9063-9156           c
            1347-1475           AC117384.5         7822-7950           c
            1476-1574           AC117384.5         6936-7034           c
            1575-1655           AC117384.5         4169-4249           c
            1656-1759           AC117384.5         2740-2843           c
            1760-1818           AC106724.3         40249-40307         c
            1819-1938           AC106724.3         33676-33795         c
            1939-2072           AC106724.3         32072-32205         c
            2073-2138           AC106724.3         31900-31965         c
            2139-2234           AC106724.3         28485-28580         c
            2235-2311           AC106724.3         28085-28161         c
            2312-5656           AC106724.3         16993-20337         c
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.2"
     gene            1..5656
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /note="membrane metalloendopeptidase"
                     /db_xref="GeneID:4311"
                     /db_xref="HGNC:HGNC:7154"
                     /db_xref="MIM:120520"
     exon            1..148
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            149..318
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..2411
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /EC_number="3.4.24.11"
                     /note="isoform a is encoded by transcript variant 2b;
                     neprilysin; membrane metallo-endopeptidase (neutral
                     endopeptidase, enkephalinase, CALLA, CD10);
                     atriopeptidase; common acute lymphocytic leukemia antigen;
                     neutral endopeptidase 24.11; skin fibroblast elastase;
                     neprilysin-411; neprilysin-390"
                     /codon_start=1
                     /product="neprilysin isoform a"
                     /protein_id="NP_009220.2"
                     /db_xref="CCDS:CCDS3172.1"
                     /db_xref="GeneID:4311"
                     /db_xref="HGNC:HGNC:7154"
                     /db_xref="MIM:120520"
                     /translation="MGKSESQMDITDINTPKPKKKQRWTPLEISLSVLVLLLTIIAVT
                     MIALYATYDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSS
                     RYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKL
                     LPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIH
                     IDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKV
                     MELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTV
                     NISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRN
                     AFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREV
                     FIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYF
                     ENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFS
                     AQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVY
                     QYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNH
                     KQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSY
                     MNPEKKCRVW"
     exon            319..354
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            355..516
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            517..597
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            598..693
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            694..812
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            813..878
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            879..1013
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1115
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1252
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1346
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1475
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..1574
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..1655
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1759
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1818
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1938
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2072
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2138
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2139..2234
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2311
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2312..5656
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5631..5636
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /note="hexamer: AATAAA"
     polyA_site      5656
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /note="major polyA site"
ORIGIN      
        1 atagttccct gcggcctctg ccttggggag ttatgttttg ttaccgagat ccgcgctacc
       61 agattgcacc ggggctgatt tgggggctgg gaatttgcca ttctgctgta cagacactga
      121 tttttttttc ttctttttaa aaagcaagat tttaggtgat gggcaagtca gaaagtcaga
      181 tggatataac tgatatcaac actccaaagc caaagaagaa acagcgatgg actccactgg
      241 agatcagcct ctcggtcctt gtcctgctcc tcaccatcat agctgtgaca atgatcgcac
      301 tctatgcaac ctacgatgat ggtatttgca agtcatcaga ctgcataaaa tcagctgctc
      361 gactgatcca aaacatggat gccaccactg agccttgtac agactttttc aaatatgctt
      421 gcggaggctg gttgaaacgt aatgtcattc ccgagaccag ctcccgttac ggcaactttg
      481 acattttaag agatgaacta gaagtcgttt tgaaagatgt ccttcaagaa cccaaaactg
      541 aagatatagt agcagtgcag aaagcaaaag cattgtacag gtcttgtata aatgaatctg
      601 ctattgatag cagaggtgga gaacctctac tcaaactgtt accagacata tatgggtggc
      661 cagtagcaac agaaaactgg gagcaaaaat atggtgcttc ttggacagct gaaaaagcta
      721 ttgcacaact gaattctaaa tatgggaaaa aagtccttat taatttgttt gttggcactg
      781 atgataagaa ttctgtgaat catgtaattc atattgacca acctcgactt ggcctccctt
      841 ctagagatta ctatgaatgc actggaatct ataaagaggc ttgtacagca tatgtggatt
      901 ttatgatttc tgtggccaga ttgattcgtc aggaagaaag attgcccatc gatgaaaacc
      961 agcttgcttt ggaaatgaat aaagttatgg aattggaaaa agaaattgcc aatgctacgg
     1021 ctaaacctga agatcgaaat gatccaatgc ttctgtataa caagatgaca ttggcccaga
     1081 tccaaaataa cttttcacta gagatcaatg ggaagccatt cagctggttg aatttcacaa
     1141 atgaaatcat gtcaactgtg aatattagta ttacaaatga ggaagatgtg gttgtttatg
     1201 ctccagaata tttaaccaaa cttaagccca ttcttaccaa atattctgcc agagatcttc
     1261 aaaatttaat gtcctggaga ttcataatgg atcttgtaag cagcctcagc cgaacctaca
     1321 aggagtccag aaatgctttc cgcaaggccc tttatggtac aacctcagaa acagcaactt
     1381 ggagacgttg tgcaaactat gtcaatggga atatggaaaa tgctgtgggg aggctttatg
     1441 tggaagcagc atttgctgga gagagtaaac atgtggtcga ggatttgatt gcacagatcc
     1501 gagaagtttt tattcagact ttagatgacc tcacttggat ggatgccgag acaaaaaaga
     1561 gagctgaaga aaaggcctta gcaattaaag aaaggatcgg ctatcctgat gacattgttt
     1621 caaatgataa caaactgaat aatgagtacc tcgagttgaa ctacaaagaa gatgaatact
     1681 tcgagaacat aattcaaaat ttgaaattca gccaaagtaa acaactgaag aagctccgag
     1741 aaaaggtgga caaagatgag tggataagtg gagcagctgt agtcaatgca ttttactctt
     1801 caggaagaaa tcagatagtc ttcccagccg gcattctgca gccccccttc tttagtgccc
     1861 agcagtccaa ctcattgaac tatgggggca tcggcatggt cataggacac gaaatcaccc
     1921 atggcttcga tgacaatggc agaaacttta acaaagatgg agacctcgtt gactggtgga
     1981 ctcaacagtc tgcaagtaac tttaaggagc aatcccagtg catggtgtat cagtatggaa
     2041 acttttcctg ggacctggca ggtggacagc accttaatgg aattaataca ctgggagaaa
     2101 acattgctga taatggaggt cttggtcaag catacagagc ctatcagaat tatattaaaa
     2161 agaatggcga agaaaaatta cttcctggac ttgacctaaa tcacaaacaa ctatttttct
     2221 tgaactttgc acaggtgtgg tgtggaacct ataggccaga gtatgcggtt aactccatta
     2281 aaacagatgt gcacagtcca ggcaatttca ggattattgg gactttgcag aactctgcag
     2341 agttttcaga agcctttcac tgccgcaaga attcatacat gaatccagaa aagaagtgcc
     2401 gggtttggtg atcttcaaaa gaagcattgc agcccttggc tagacttgcc aacaccacag
     2461 aaatggggaa ttctctaatc gaaagaaaat gggccctagg ggtcactgta ctgacttgag
     2521 ggtgattaac agagagggca ccatcacaat acagataaca ttaggttgtc ctagaaaggg
     2581 tgtggaggga ggaagggggt ctaaggtcta tcaagtcaat catttctcac tgtgtacata
     2641 atgcttaatt tctaaagata atattactgt ttatttctgt ttctcatatg gtctaccagt
     2701 ttgctgatgt ccctagaaaa caatgcaaaa cctttgaggt agaccaggat ttctaatcaa
     2761 aagggaaaag aagatgttga agaatacagt taggcaccag aagaacagta ggtgacacta
     2821 tagtttaaaa cacattgcct aactactagt ttttactttt atttgcaaca tttacagtcc
     2881 ttcaaaatcc ttccaaagaa ttcttataca cattggggcc ttggagctta catagtttta
     2941 aactcatttt tgccatacat cagttattca ttctgtgatc atttatttta agcactctta
     3001 aagcaaaaaa tgaatgtcta aaattgtttt ttgttgtacc tgctttgact gatgctgaga
     3061 ttcttcaggc ttcctgcaat tttctaagca atttcttgct ctatctctca aaacttggta
     3121 tttttcagag atttatataa atgtaaaaat aataattttt atatttaatt attaactaca
     3181 tttatgagta actattatta taggtaatca atgaatattg aagtttcagc ttaaaataaa
     3241 cagttgtgaa ccaagatcta taaagcgata tacagatgaa aatttgagac tatttaaact
     3301 tataaatcat attgatgaaa agatttaagc acaaacttta gggtaaaaat tgccattgga
     3361 cagttgtcta gagatatata tacttgtggt tttcaaattg gactttcaaa attaaatctg
     3421 tccctgagag tgtctctgat aaaagggcaa atctgcacct atgtagctct gcatctcctg
     3481 tcttttcagg tttgtcatca gatggaaata ttttgataat aaattgaaat tgtgaactca
     3541 ttgctcccta agactgtgac aactgtctaa ctttagaagt gcatttctga atagaaatgg
     3601 gaggcctctg atggaccttc tagaattata agtcacaaag agttctggaa aagaactgtt
     3661 tactgcttga taggaattca tcttttgagg cttctgttcc tctcttttcc tgttgtattg
     3721 actattttcg ttcattactt gattaagatt ttacaaaaga ggagcacttc caaaattctt
     3781 atttttccta acaaaagatg aaagcaggga atttctatct aaatgatgag tattagttcc
     3841 ctgtctcttg aaaaatgccc atttgccttt aaaaaaaaaa gttacagaaa tactataaca
     3901 tatgtacata aattgcataa agcataagta tacagttcaa taaacttaac tttaactgaa
     3961 caatggccct gtagccagca cctgtaagaa acagagcagt accagcgctc taaaagcacc
     4021 tccttgtcac tttattactc ccagaacaac aactatcctg acttctaata tcattcacta
     4081 gctttgcctg gttttgtctt ttatgcagat agaatcaatc agtatgtatt cttttgtgcc
     4141 tggcttcttt ctctcagcct tacatttgtg agattcctct gtattgtgct gattgtggat
     4201 cttttcattc tcattgcaga ataatgttct attgtgggac ttattacaat ttgttcatcc
     4261 tattgttgat gggcacttga gaactttcca ttttggcgct attacaaata gtgcaactat
     4321 gaatgtactg catgttacca tcttacttga gcctttaatg gacttatttc ttcaaatcct
     4381 tccaaaaatt attataagca ttgaaattat agtttcaagc caactgtgga tacccttacc
     4441 ctttcctcct ttatcacaac caccgttaca agtatactta tatttcccta aaatacattt
     4501 aaaacttacc taagtgacat ttgtagttgg agtaatagga gcttccagct ctaataaaac
     4561 agctgtctct aacttatttt atttccatca tgtcagagca ggtgaagagc cagaagtgaa
     4621 gagtgactag tacaaattat aaaaagccac tagactcttc actgttagct ttttaaaaca
     4681 ttaggctccc atccctatgg aggaacaact ctccagtgcc tggatcccct ctgtctacaa
     4741 atataagatt ttctgggcct aaaggataga tcaaagtcaa aaatagcaat gcctccctat
     4801 ccctcacaca tccagacatc atgaatttta catggtactc ttgttgagtt ctgtagagcc
     4861 ttctgatgtc tctaaagcac taccgattct ttggagttgt cacatcagat aagacatatc
     4921 tctaattcca tccataaatc cagttctact atggctgagt tctggtcaaa gaaagaaagt
     4981 ttagaagctg agacacaaag ggttgggagc tgatgaaact cacaaatgat ggtaggaaga
     5041 agctctcgac aatacccgtt ggcaaggagt ctgcctccat gctgcagtgt tcgagtggat
     5101 tgtaggtgca agatggaaag gattgtaggt gcaagctgtc cagagaaaag agtccttgtt
     5161 ccagccctat tctgccactc ctgacagggt gaccttgggt atttgcaata ttcctttggg
     5221 cctctgcttc tctcacctaa aaaaagagaa ttagattata ttggtggttc tcagcaagag
     5281 aaggagtatg tgtccaatgc tgccttccca tgaatctgtc tcccagttat gaatcagtgg
     5341 gcaggataaa ctgaaaactc ccatttacgt gtctgaatcg agtgagacaa aattttagtc
     5401 caaataacaa gtaccaaagt tttatcaagt ttgggtctgt gctgctgtta ctgttaacca
     5461 tttaagtggg gcaaaacctt gctaattttc tcaaaagcat ttatcattct tgttgccaca
     5521 gctggagctc tcaaactaaa agacatttgt tattttggaa agaagaaaga ctctattctc
     5581 aaagtttcct aatcagaaat ttttatcagt ttccagtctc aaaaatacaa aataaaaaca
     5641 aacgttttta atacta
//
