LOCUS       NM_007288               5818 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens membrane metalloendopeptidase (MME), transcript
            variant 2a, mRNA.
ACCESSION   NM_007288
VERSION     NM_007288.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Zhang,B., Gang,Q., Meng,L., Li,Z., Chu,X., Wu,H., Yang,J., Huang,B.
            and Du,K.
  TITLE     A novel variant of biallelic MME gene associated with autosomal
            recessive late-onset distal hereditary motor neuropathy in Chinese
            families
  JOURNAL   BMC Med Genomics 17 (1), 223 (2024)
   PUBMED   39232784
  REMARK    GeneRIF: A novel variant of biallelic MME gene associated with
            autosomal recessive late-onset distal hereditary motor neuropathy
            in Chinese families.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Grosz,B.R., Parmar,J.M., Ellis,M., Bryen,S., Simons,C.,
            Reis,A.L.M., Stevanovski,I., Deveson,I.W., Nicholson,G., Laing,N.,
            Wallis,M., Ravenscroft,G., Kumar,K.R., Vucic,S. and Kennerson,M.L.
  TITLE     A deep intronic variant in MME causes autosomal recessive
            Charcot-Marie-Tooth neuropathy through aberrant splicing
  JOURNAL   J Peripher Nerv Syst 29 (2), 262-274 (2024)
   PUBMED   38860315
  REMARK    GeneRIF: A deep intronic variant in MME causes autosomal recessive
            Charcot-Marie-Tooth neuropathy through aberrant splicing.
REFERENCE   3  (bases 1 to 5818)
  AUTHORS   Shigematsu,K., Komori,N., Ideno,M. and Yamagishi,H.
  TITLE     'Evaluation of neprilysin activity in Adipose-Derived stem cells
            from Alzheimer's disease patients'
  JOURNAL   Neurosci Lett 825, 137705 (2024)
   PUBMED   38428725
  REMARK    GeneRIF: 'Evaluation of neprilysin activity in Adipose-Derived stem
            cells from Alzheimer's disease patients'.
REFERENCE   4  (bases 1 to 5818)
  AUTHORS   Chen,H., Huang,Z., Lei,A., Yu,X., Shen,M. and Wu,D.
  TITLE     miRNA-211-5p inhibition enhances the protective effect of
            hucMSC-derived exosome in Abeta1-40 -induced SH-SY5Y cells by
            increasing NEP expression
  JOURNAL   J Biochem Mol Toxicol 38 (1), e23624 (2024)
   PUBMED   38229323
  REMARK    GeneRIF: miRNA-211-5p inhibition enhances the protective effect of
            hucMSC-derived exosome in Abeta1-40 -induced SH-SY5Y cells by
            increasing NEP expression.
REFERENCE   5  (bases 1 to 5818)
  AUTHORS   Bird,T.D.
  TITLE     Charcot-Marie-Tooth Neuropathy Type 2 - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301462
REFERENCE   6  (bases 1 to 5818)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
REFERENCE   7  (bases 1 to 5818)
  AUTHORS   Shipp,M.A., Tarr,G.E., Chen,C.Y., Switzer,S.N., Hersh,L.B.,
            Stein,H., Sunday,M.E. and Reinherz,E.L.
  TITLE     CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides
            and regulates the growth of small cell carcinomas of the lung
  JOURNAL   Proc Natl Acad Sci U S A 88 (23), 10662-10666 (1991)
   PUBMED   1660144
REFERENCE   8  (bases 1 to 5818)
  AUTHORS   Vijayaraghavan,J., Scicli,A.G., Carretero,O.A., Slaughter,C.,
            Moomaw,C. and Hersh,L.B.
  TITLE     The hydrolysis of endothelins by neutral endopeptidase 24.11
            (enkephalinase)
  JOURNAL   J Biol Chem 265 (24), 14150-14155 (1990)
   PUBMED   2201681
REFERENCE   9  (bases 1 to 5818)
  AUTHORS   Barker,P.E., Shipp,M.A., D'Adamio,L., Masteller,E.L. and
            Reinherz,E.L.
  TITLE     The common acute lymphoblastic leukemia antigen gene maps to
            chromosomal region 3 (q21-q27)
  JOURNAL   J Immunol 142 (1), 283-287 (1989)
   PUBMED   2521237
REFERENCE   10 (bases 1 to 5818)
  AUTHORS   Skidgel,R.A.
  TITLE     Characterization of the metabolism of substance P and neurotensin
            by human angiotensin I converting enzyme and 'enkephalinase'
  JOURNAL   Prog Clin Biol Res 192, 371-378 (1985)
   PUBMED   2417254
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC117384.5 and AC106724.3.
            
            On Aug 17, 2017 this sequence version replaced NM_007288.2.
            
            Summary: The protein encoded by this gene is a type II
            transmembrane glycoprotein and a common acute lymphocytic leukemia
            antigen that is an important cell surface marker in the diagnosis
            of human acute lymphocytic leukemia (ALL). The encoded protein is
            present on leukemic cells of pre-B phenotype, which represent 85%
            of cases of ALL. This protein is not restricted to leukemic cells,
            however, and is found on a variety of normal tissues. The protein
            is a neutral endopeptidase that cleaves peptides at the amino side
            of hydrophobic residues and inactivates several peptide hormones
            including glucagon, enkephalins, substance P, neurotensin,
            oxytocin, and bradykinin. [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (2a) contains an alternate 5' UTR
            exon, compared to variant 2b. Variants 1, 1bis, 2a, 2b, 3, and 4
            all encode the same isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853565.21160.1,
                                           SRR11853567.21873.1 [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-285               AC117384.5         71098-71382         c
            286-455             AC117384.5         67066-67235         c
            456-491             AC117384.5         66299-66334         c
            492-653             AC117384.5         36238-36399         c
            654-734             AC117384.5         34834-34914         c
            735-830             AC117384.5         34634-34729         c
            831-949             AC117384.5         34407-34525         c
            950-1015            AC117384.5         32582-32647         c
            1016-1150           AC117384.5         13157-13291         c
            1151-1252           AC117384.5         11101-11202         c
            1253-1389           AC117384.5         9266-9402           c
            1390-1483           AC117384.5         9063-9156           c
            1484-1612           AC117384.5         7822-7950           c
            1613-1711           AC117384.5         6936-7034           c
            1712-1792           AC117384.5         4169-4249           c
            1793-1896           AC117384.5         2740-2843           c
            1897-1955           AC106724.3         40249-40307         c
            1956-2075           AC106724.3         33676-33795         c
            2076-2209           AC106724.3         32072-32205         c
            2210-2275           AC106724.3         31900-31965         c
            2276-2371           AC106724.3         28485-28580         c
            2372-2448           AC106724.3         28085-28161         c
            2449-5818           AC106724.3         16968-20337         c
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q25.2"
     gene            1..5818
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /note="membrane metalloendopeptidase"
                     /db_xref="GeneID:4311"
                     /db_xref="HGNC:HGNC:7154"
                     /db_xref="MIM:120520"
     exon            1..285
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            286..455
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     CDS             296..2548
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /EC_number="3.4.24.11"
                     /note="isoform a is encoded by transcript variant 2a;
                     neprilysin; membrane metallo-endopeptidase (neutral
                     endopeptidase, enkephalinase, CALLA, CD10);
                     atriopeptidase; common acute lymphocytic leukemia antigen;
                     neutral endopeptidase 24.11; skin fibroblast elastase;
                     neprilysin-411; neprilysin-390"
                     /codon_start=1
                     /product="neprilysin isoform a"
                     /protein_id="NP_009219.2"
                     /db_xref="CCDS:CCDS3172.1"
                     /db_xref="GeneID:4311"
                     /db_xref="HGNC:HGNC:7154"
                     /db_xref="MIM:120520"
                     /translation="MGKSESQMDITDINTPKPKKKQRWTPLEISLSVLVLLLTIIAVT
                     MIALYATYDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSS
                     RYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKL
                     LPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIH
                     IDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKV
                     MELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTV
                     NISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRN
                     AFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREV
                     FIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYF
                     ENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFS
                     AQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVY
                     QYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNH
                     KQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSY
                     MNPEKKCRVW"
     exon            456..491
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            492..653
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            654..734
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            735..830
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            831..949
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1015
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1150
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1252
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1389
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1483
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1612
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1711
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1792
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1793..1896
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..1955
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2075
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2209
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2275
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2371
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2372..2448
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2449..5818
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5768..5773
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /note="hexamer: AATAAA"
     polyA_site      5793
                     /gene="MME"
                     /gene_synonym="CALLA; CD10; CMT2T; NEP; SCA43; SFE"
                     /note="major polyA site"
ORIGIN      
        1 aggagagcga gctgagggag aaaggtccaa agggcgcgag cgcccagggc gctgggagcc
       61 cgtgggacgg gcacggggag ggcagagcca gccgagggga gcggctgccg ggaggtgcgc
      121 tgagcagggt ccgcagctaa ggtccagcgc gatccgagcg cccaggaccc tgcggccgtg
      181 gaggaggcgt gccctgggaa ggagccgcta ctgggacctg aagagtgagg ggagggagag
      241 gggcccagag ggtgacctgg aggagggctc tggaagtcac gtcagatttt aggtgatggg
      301 caagtcagaa agtcagatgg atataactga tatcaacact ccaaagccaa agaagaaaca
      361 gcgatggact ccactggaga tcagcctctc ggtccttgtc ctgctcctca ccatcatagc
      421 tgtgacaatg atcgcactct atgcaaccta cgatgatggt atttgcaagt catcagactg
      481 cataaaatca gctgctcgac tgatccaaaa catggatgcc accactgagc cttgtacaga
      541 ctttttcaaa tatgcttgcg gaggctggtt gaaacgtaat gtcattcccg agaccagctc
      601 ccgttacggc aactttgaca ttttaagaga tgaactagaa gtcgttttga aagatgtcct
      661 tcaagaaccc aaaactgaag atatagtagc agtgcagaaa gcaaaagcat tgtacaggtc
      721 ttgtataaat gaatctgcta ttgatagcag aggtggagaa cctctactca aactgttacc
      781 agacatatat gggtggccag tagcaacaga aaactgggag caaaaatatg gtgcttcttg
      841 gacagctgaa aaagctattg cacaactgaa ttctaaatat gggaaaaaag tccttattaa
      901 tttgtttgtt ggcactgatg ataagaattc tgtgaatcat gtaattcata ttgaccaacc
      961 tcgacttggc ctcccttcta gagattacta tgaatgcact ggaatctata aagaggcttg
     1021 tacagcatat gtggatttta tgatttctgt ggccagattg attcgtcagg aagaaagatt
     1081 gcccatcgat gaaaaccagc ttgctttgga aatgaataaa gttatggaat tggaaaaaga
     1141 aattgccaat gctacggcta aacctgaaga tcgaaatgat ccaatgcttc tgtataacaa
     1201 gatgacattg gcccagatcc aaaataactt ttcactagag atcaatggga agccattcag
     1261 ctggttgaat ttcacaaatg aaatcatgtc aactgtgaat attagtatta caaatgagga
     1321 agatgtggtt gtttatgctc cagaatattt aaccaaactt aagcccattc ttaccaaata
     1381 ttctgccaga gatcttcaaa atttaatgtc ctggagattc ataatggatc ttgtaagcag
     1441 cctcagccga acctacaagg agtccagaaa tgctttccgc aaggcccttt atggtacaac
     1501 ctcagaaaca gcaacttgga gacgttgtgc aaactatgtc aatgggaata tggaaaatgc
     1561 tgtggggagg ctttatgtgg aagcagcatt tgctggagag agtaaacatg tggtcgagga
     1621 tttgattgca cagatccgag aagtttttat tcagacttta gatgacctca cttggatgga
     1681 tgccgagaca aaaaagagag ctgaagaaaa ggccttagca attaaagaaa ggatcggcta
     1741 tcctgatgac attgtttcaa atgataacaa actgaataat gagtacctcg agttgaacta
     1801 caaagaagat gaatacttcg agaacataat tcaaaatttg aaattcagcc aaagtaaaca
     1861 actgaagaag ctccgagaaa aggtggacaa agatgagtgg ataagtggag cagctgtagt
     1921 caatgcattt tactcttcag gaagaaatca gatagtcttc ccagccggca ttctgcagcc
     1981 ccccttcttt agtgcccagc agtccaactc attgaactat gggggcatcg gcatggtcat
     2041 aggacacgaa atcacccatg gcttcgatga caatggcaga aactttaaca aagatggaga
     2101 cctcgttgac tggtggactc aacagtctgc aagtaacttt aaggagcaat cccagtgcat
     2161 ggtgtatcag tatggaaact tttcctggga cctggcaggt ggacagcacc ttaatggaat
     2221 taatacactg ggagaaaaca ttgctgataa tggaggtctt ggtcaagcat acagagccta
     2281 tcagaattat attaaaaaga atggcgaaga aaaattactt cctggacttg acctaaatca
     2341 caaacaacta tttttcttga actttgcaca ggtgtggtgt ggaacctata ggccagagta
     2401 tgcggttaac tccattaaaa cagatgtgca cagtccaggc aatttcagga ttattgggac
     2461 tttgcagaac tctgcagagt tttcagaagc ctttcactgc cgcaagaatt catacatgaa
     2521 tccagaaaag aagtgccggg tttggtgatc ttcaaaagaa gcattgcagc ccttggctag
     2581 acttgccaac accacagaaa tggggaattc tctaatcgaa agaaaatggg ccctaggggt
     2641 cactgtactg acttgagggt gattaacaga gagggcacca tcacaataca gataacatta
     2701 ggttgtccta gaaagggtgt ggagggagga agggggtcta aggtctatca agtcaatcat
     2761 ttctcactgt gtacataatg cttaatttct aaagataata ttactgttta tttctgtttc
     2821 tcatatggtc taccagtttg ctgatgtccc tagaaaacaa tgcaaaacct ttgaggtaga
     2881 ccaggatttc taatcaaaag ggaaaagaag atgttgaaga atacagttag gcaccagaag
     2941 aacagtaggt gacactatag tttaaaacac attgcctaac tactagtttt tacttttatt
     3001 tgcaacattt acagtccttc aaaatccttc caaagaattc ttatacacat tggggccttg
     3061 gagcttacat agttttaaac tcatttttgc catacatcag ttattcattc tgtgatcatt
     3121 tattttaagc actcttaaag caaaaaatga atgtctaaaa ttgttttttg ttgtacctgc
     3181 tttgactgat gctgagattc ttcaggcttc ctgcaatttt ctaagcaatt tcttgctcta
     3241 tctctcaaaa cttggtattt ttcagagatt tatataaatg taaaaataat aatttttata
     3301 tttaattatt aactacattt atgagtaact attattatag gtaatcaatg aatattgaag
     3361 tttcagctta aaataaacag ttgtgaacca agatctataa agcgatatac agatgaaaat
     3421 ttgagactat ttaaacttat aaatcatatt gatgaaaaga tttaagcaca aactttaggg
     3481 taaaaattgc cattggacag ttgtctagag atatatatac ttgtggtttt caaattggac
     3541 tttcaaaatt aaatctgtcc ctgagagtgt ctctgataaa agggcaaatc tgcacctatg
     3601 tagctctgca tctcctgtct tttcaggttt gtcatcagat ggaaatattt tgataataaa
     3661 ttgaaattgt gaactcattg ctccctaaga ctgtgacaac tgtctaactt tagaagtgca
     3721 tttctgaata gaaatgggag gcctctgatg gaccttctag aattataagt cacaaagagt
     3781 tctggaaaag aactgtttac tgcttgatag gaattcatct tttgaggctt ctgttcctct
     3841 cttttcctgt tgtattgact attttcgttc attacttgat taagatttta caaaagagga
     3901 gcacttccaa aattcttatt tttcctaaca aaagatgaaa gcagggaatt tctatctaaa
     3961 tgatgagtat tagttccctg tctcttgaaa aatgcccatt tgcctttaaa aaaaaaagtt
     4021 acagaaatac tataacatat gtacataaat tgcataaagc ataagtatac agttcaataa
     4081 acttaacttt aactgaacaa tggccctgta gccagcacct gtaagaaaca gagcagtacc
     4141 agcgctctaa aagcacctcc ttgtcacttt attactccca gaacaacaac tatcctgact
     4201 tctaatatca ttcactagct ttgcctggtt ttgtctttta tgcagataga atcaatcagt
     4261 atgtattctt ttgtgcctgg cttctttctc tcagccttac atttgtgaga ttcctctgta
     4321 ttgtgctgat tgtggatctt ttcattctca ttgcagaata atgttctatt gtgggactta
     4381 ttacaatttg ttcatcctat tgttgatggg cacttgagaa ctttccattt tggcgctatt
     4441 acaaatagtg caactatgaa tgtactgcat gttaccatct tacttgagcc tttaatggac
     4501 ttatttcttc aaatccttcc aaaaattatt ataagcattg aaattatagt ttcaagccaa
     4561 ctgtggatac ccttaccctt tcctccttta tcacaaccac cgttacaagt atacttatat
     4621 ttccctaaaa tacatttaaa acttacctaa gtgacatttg tagttggagt aataggagct
     4681 tccagctcta ataaaacagc tgtctctaac ttattttatt tccatcatgt cagagcaggt
     4741 gaagagccag aagtgaagag tgactagtac aaattataaa aagccactag actcttcact
     4801 gttagctttt taaaacatta ggctcccatc cctatggagg aacaactctc cagtgcctgg
     4861 atcccctctg tctacaaata taagattttc tgggcctaaa ggatagatca aagtcaaaaa
     4921 tagcaatgcc tccctatccc tcacacatcc agacatcatg aattttacat ggtactcttg
     4981 ttgagttctg tagagccttc tgatgtctct aaagcactac cgattctttg gagttgtcac
     5041 atcagataag acatatctct aattccatcc ataaatccag ttctactatg gctgagttct
     5101 ggtcaaagaa agaaagttta gaagctgaga cacaaagggt tgggagctga tgaaactcac
     5161 aaatgatggt aggaagaagc tctcgacaat acccgttggc aaggagtctg cctccatgct
     5221 gcagtgttcg agtggattgt aggtgcaaga tggaaaggat tgtaggtgca agctgtccag
     5281 agaaaagagt ccttgttcca gccctattct gccactcctg acagggtgac cttgggtatt
     5341 tgcaatattc ctttgggcct ctgcttctct cacctaaaaa aagagaatta gattatattg
     5401 gtggttctca gcaagagaag gagtatgtgt ccaatgctgc cttcccatga atctgtctcc
     5461 cagttatgaa tcagtgggca ggataaactg aaaactccca tttacgtgtc tgaatcgagt
     5521 gagacaaaat tttagtccaa ataacaagta ccaaagtttt atcaagtttg ggtctgtgct
     5581 gctgttactg ttaaccattt aagtggggca aaaccttgct aattttctca aaagcattta
     5641 tcattcttgt tgccacagct ggagctctca aactaaaaga catttgttat tttggaaaga
     5701 agaaagactc tattctcaaa gtttcctaat cagaaatttt tatcagtttc cagtctcaaa
     5761 aatacaaaat aaaaacaaac gtttttaata ctattgcttt tatgcctagt caactctg
//
