LOCUS       NM_012213              13054 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens malonyl-CoA decarboxylase (MLYCD), mRNA; nuclear gene
            for mitochondrial product.
ACCESSION   NM_012213
VERSION     NM_012213.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 13054)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 13054)
  AUTHORS   Ling,B., Liu,Y., Li,X., Wang,Z. and Bi,S.
  TITLE     Identification of the active site of human mitochondrial
            malonyl-coenzyme a decarboxylase: A combined computational study
  JOURNAL   Proteins 84 (6), 792-802 (2016)
   PUBMED   26948533
  REMARK    GeneRIF: To identify the active site of MCD, molecular docking and
            molecular dynamics simulations were performed to explore the
            interactions of human mitochondrial MCD (HmMCD) and CoA
            derivatives. The findings reveal that the active site of HmMCD
            indeed resides in the prominent groove which resembles that of
            curacin A.
REFERENCE   4  (bases 1 to 13054)
  AUTHORS   Liu,H., Tan,D., Han,L., Ye,J., Qiu,W., Gu,X. and Zhang,H.
  TITLE     A new case of malonyl-CoA decarboxylase deficiency with mild
            clinical features
  JOURNAL   Am J Med Genet A 170A (5), 1347-1351 (2016)
   PUBMED   26858006
  REMARK    GeneRIF: Our result expands the phenotype of malonyl-CoA
            decarboxylase deficiency and suggests attentions should be paid to
            the mild form of disorders, for example, malonyl-CoA decarboxylase
            deficiency, which usually present a severe disease course.
REFERENCE   5  (bases 1 to 13054)
  AUTHORS   Froese,D.S., Forouhar,F., Tran,T.H., Vollmar,M., Kim,Y.S., Lew,S.,
            Neely,H., Seetharaman,J., Shen,Y., Xiao,R., Acton,T.B.,
            Everett,J.K., Cannone,G., Puranik,S., Savitsky,P., Krojer,T.,
            Pilka,E.S., Kiyani,W., Lee,W.H., Marsden,B.D., von Delft,F.,
            Allerston,C.K., Spagnolo,L., Gileadi,O., Montelione,G.T.,
            Oppermann,U., Yue,W.W. and Tong,L.
  TITLE     Crystal structures of malonyl-coenzyme A decarboxylase provide
            insights into its catalytic mechanism and disease-causing mutations
  JOURNAL   Structure 21 (7), 1182-1192 (2013)
   PUBMED   23791943
  REMARK    GeneRIF: The MLYCD catalytic domain is structurally homologous to
            those of the GCN5-related N-acetyltransferase superfamily.
REFERENCE   6  (bases 1 to 13054)
  AUTHORS   Zhou,D., Yuen,P., Chu,D., Thon,V., McConnell,S., Brown,S.,
            Tsang,A., Pena,M., Russell,A., Cheng,J.F., Nadzan,A.M.,
            Barbosa,M.S., Dyck,J.R., Lopaschuk,G.D. and Yang,G.
  TITLE     Expression, purification, and characterization of human malonyl-CoA
            decarboxylase
  JOURNAL   Protein Expr Purif 34 (2), 261-269 (2004)
   PUBMED   15003260
REFERENCE   7  (bases 1 to 13054)
  AUTHORS   Wightman,P.J., Santer,R., Ribes,A., Dougherty,F., McGill,N.,
            Thorburn,D.R. and FitzPatrick,D.R.
  TITLE     MLYCD mutation analysis: evidence for protein mistargeting as a
            cause of MLYCD deficiency
  JOURNAL   Hum Mutat 22 (4), 288-300 (2003)
   PUBMED   12955715
  REMARK    GeneRIF: Malonyl-CoA decarboxylase deficiency may result from MLYCD
            mutations that result in protein mistargeting.
REFERENCE   8  (bases 1 to 13054)
  AUTHORS   Sacksteder,K.A., Morrell,J.C., Wanders,R.J., Matalon,R. and
            Gould,S.J.
  TITLE     MCD encodes peroxisomal and cytoplasmic forms of malonyl-CoA
            decarboxylase and is mutated in malonyl-CoA decarboxylase
            deficiency
  JOURNAL   J Biol Chem 274 (35), 24461-24468 (1999)
   PUBMED   10455107
REFERENCE   9  (bases 1 to 13054)
  AUTHORS   FitzPatrick,D.R., Hill,A., Tolmie,J.L., Thorburn,D.R. and
            Christodoulou,J.
  TITLE     The molecular basis of malonyl-CoA decarboxylase deficiency
  JOURNAL   Am J Hum Genet 65 (2), 318-326 (1999)
   PUBMED   10417274
REFERENCE   10 (bases 1 to 13054)
  AUTHORS   Gao,J., Waber,L., Bennett,M.J., Gibson,K.M. and Cohen,J.C.
  TITLE     Cloning and mutational analysis of human malonyl-coenzyme A
            decarboxylase
  JOURNAL   J Lipid Res 40 (1), 178-182 (1999)
   PUBMED   9869665
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009119.10 and BC052592.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_012213.2.
            
            Summary: The product of this gene catalyzes the breakdown of
            malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an
            intermediate in fatty acid biosynthesis, and also inhibits the
            transport of fatty acyl CoAs into mitochondria. Consequently, the
            encoded protein acts to increase the rate of fatty acid oxidation.
            It is found in mitochondria, peroxisomes, and the cytoplasm.
            Mutations in this gene result in malonyl-CoA decarboyxlase
            deficiency. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052592.1, AF153679.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000262430.6/
                                                  ENSP00000262430.4
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC009119.10        128614-128623
            11-2205             BC052592.1         1-2195
            2206-13054          AC009119.10        145682-156530
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q23.3"
     gene            1..13054
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /note="malonyl-CoA decarboxylase"
                     /db_xref="GeneID:23417"
                     /db_xref="HGNC:HGNC:7150"
                     /db_xref="MIM:606761"
     exon            1..558
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1512
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /EC_number="4.1.1.9"
                     /note="malonyl coenzyme A decarboxylase"
                     /codon_start=1
                     /product="malonyl-CoA decarboxylase, mitochondrial"
                     /protein_id="NP_036345.2"
                     /db_xref="CCDS:CCDS42206.1"
                     /db_xref="GeneID:23417"
                     /db_xref="HGNC:HGNC:7150"
                     /db_xref="MIM:606761"
                     /translation="MRGFGPGLTARRLLPLRLPPRPPGPRLASGQAAGALERAMDELL
                     RRAVPPTPAYELREKTPAPAEGQCADFVSFYGGLAETAQRAELLGRLARGFGVDHGQV
                     AEQSAGVLHLRQQQREAAVLLQAEDRLRYALVPRYRGLFHHISKLDGGVRFLVQLRAD
                     LLEAQALKLVEGPDVREMNGVLKGMLSEWFSSGFLNLERVTWHSPCEVLQKISEAEAV
                     HPVKNWMDMKRRVGPYRRCYFFSHCSTPGEPLVVLHVALTGDISSNIQAIVKEHPPSE
                     TEEKNKITAAIFYSISLTQQGLQGVELGTFLIKRVVKELQREFPHLGVFSSLSPIPGF
                     TKWLLGLLNSQTKEHGRNELFTDSECKEISEITGGPINETLKLLLSSSEWVQSEKLVR
                     ALQTPLMRLCAWYLYGEKHRGYALNPVANFHLQNGAVLWRINWMADVSLRGITGSCGL
                     MANYRYFLEETGPNSTSYLGSKIIKASEQVLSLVAQFQKNSKL"
     exon            559..671
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /inference="alignment:Splign:2.1.0"
     exon            672..828
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /inference="alignment:Splign:2.1.0"
     exon            829..978
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /inference="alignment:Splign:2.1.0"
     exon            979..13054
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2172..2177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /note="hexamer: AGTAAA"
     polyA_site      2197
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /note="major polyA site"
     regulatory      8111..8116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /note="hexamer: AATAAA"
     polyA_site      8140
                     /gene="MLYCD"
                     /gene_synonym="MCD"
     regulatory      9286..9291
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /note="hexamer: ATTAAA"
     polyA_site      9315
                     /gene="MLYCD"
                     /gene_synonym="MCD"
     regulatory      13028..13033
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /note="hexamer: AATAAA"
     regulatory      13034..13039
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MLYCD"
                     /gene_synonym="MCD"
                     /note="hexamer: AATAAA"
     polyA_site      13051
                     /gene="MLYCD"
                     /gene_synonym="MCD"
     polyA_site      13054
                     /gene="MLYCD"
                     /gene_synonym="MCD"
ORIGIN      
        1 gctccccctc ggcagctgtt gtggggcacc atgcgaggct tcgggccagg cttgacggcc
       61 aggcgtctcc tcccgctgcg gttgcccccg cggccgcccg ggccccggct ggcgagcggg
      121 caggcggccg gcgccctgga gcgggccatg gacgagctgc tgcgccgcgc ggtgccgccg
      181 acgccggcct acgagctgcg cgagaagaca ccggcgcccg ccgagggtca gtgcgcggac
      241 ttcgtgagct tctacggtgg gctggccgag acggcccagc gggccgaact gctgggccgc
      301 ctggcgcggg gcttcggcgt ggaccacggc caggtggcgg agcagagcgc cggcgtgctc
      361 catctgcgcc agcagcagcg ggaggcggcg gtgctgctgc aggccgagga ccggctgcgc
      421 tacgcgctgg tgccgcgcta tcgcggcctc ttccaccaca tcagcaagct ggacggcggc
      481 gtgcgcttcc tggtgcagct gcgggccgac ctgctggagg cgcaggccct caagctggtg
      541 gaggggccgg acgtccggga aatgaatggg gtgctgaaag gaatgctctc agaatggttt
      601 tcctccgggt tcctgaacct agaacgggtt acctggcatt caccgtgtga agtgcttcag
      661 aaaatcagtg aggctgaggc tgtgcatcct gtaaaaaact ggatggacat gaagcgccgc
      721 gttgggccct acagaaggtg ttacttcttt tctcactgtt cgacccctgg ggagcccctg
      781 gtcgttttgc acgtggcact gactggtgac atctccagca acatccaggc aatcgtgaag
      841 gaacatcctc catcagaaac agaagagaag aacaaaatca ctgctgcgat cttttattcc
      901 atcagcttga cccagcaggg actccaaggg gtggagctgg gaacattcct cataaagcga
      961 gtcgtcaagg agttgcagag agagtttcct caccttgggg tgttttcaag tctgtcacct
     1021 atacctggtt tcaccaaatg gcttctgggg cttctgaact cgcaaacgaa ggagcatggg
     1081 aggaatgaac tctttacaga ttcggaatgt aaggaaatct cggagatcac aggtggcccc
     1141 attaacgaga ccctcaagct cctcctcagc agcagcgagt gggtgcagtc ggagaagctg
     1201 gtgcgggcgc tgcagactcc gctgatgagg ctgtgcgcct ggtacctgta tggagagaag
     1261 caccgcggct acgcgctgaa ccccgtggcc aacttccacc tgcagaacgg ggcggtgctg
     1321 tggcgcatca actggatggc ggatgtgagc ctcagaggca tcaccggctc ctgcggcctg
     1381 atggccaact accgctactt cctggaggag acgggcccca acagcacctc ctacctcggc
     1441 tccaagatca tcaaagcctc tgagcaggtc ctcagcctag tggcccagtt tcaaaagaac
     1501 agcaagctct gacagtaaac ctctcctaaa gcacagggcc ccggctaaga aaacgatcat
     1561 tttcaggagg ggccgggagt tatgtatctg aagcagcttt ccaagcaaag ccaaagttga
     1621 ctgtgttctt gtcccgcagc cggtccacac tgtgaggcca ggcctcaact tccctcaccc
     1681 tgggcgtgac atgcacccag tgcaagacgg ttgtgggtgc gggtgcacac aaatgagtgg
     1741 gttgctgtgc tgtctccgga agattctgtc gttgcccttg gcctggctcc ctgcccgggg
     1801 ctggtgctgt ggtacctaca tcttcaggaa gctgtgcagc ctctgccatt gccatcccag
     1861 ggggatctgg catcctccta aggaccgggg cgcgtggccc agataagaat aggtgttcct
     1921 ttgtgctcat aaaacagaat gcggcgatgg ttgctttagc cgtttctcac catgcaatgc
     1981 agagggacaa agggctgtgc tacacttccc agttacattc tgaagctcat aaatgtatga
     2041 gaaggtttgt gattttgcac aaggtgtgtg tagtaagtta aattgttgaa cacaaaaatg
     2101 ttgctgcttg aggcataagt tggataatag gctttaaatg atcagggatt caggttcata
     2161 atgaacttca cagtaaagaa cacgtttgtt ctgtaaagca ttgaacatct gattgtgtag
     2221 tggtggtcgt ctttaagatt agagacctgg gaaggctgga atcagccagc cagcctacgg
     2281 ggagtttggg atgaaggagc ctggggtgtg ttggatgaga acaaaggtga aggaaggggc
     2341 agtcattgtg cttgtgggag gaaggcagtt gtgtttgtgt gtgtatgtgt gtatcagtgc
     2401 acatctgtgt gcatgtgcac gagcgtctct gtgtggatca gtgcacgtct gtgcgtgtgc
     2461 acgagcgtct gtggatcagt gcacgtctgt gtgcgtgtgc acgagcgtct ctgtgtgtat
     2521 cagtgcatgt ctgtgtacgt gtgcacgagc atctctggat cagtgcacgt ctgtgtgcat
     2581 gtgcccgagc gtctctgtgt ggatcagtgc acgtctgtgt gcgtgtgcac gagcgtctct
     2641 gtgtggatca gtgcacgtct gtgtgcgtgt gcacgagcat ctctgtgtgt gtcagtgcac
     2701 gtctgtgtgc gtgtgcacga gcgtctctgt gtgtatcagt gcacgtctgt gtgcgtgtgc
     2761 ccgagcgtct ctgtgtggat cagtgcacgt ctgtgtgcat gtgcacgagc gttctatgtg
     2821 gatcagtgca cgcctgtgtg cgtgtgcacg agcgtctctg tgtgtcagtg cacgtctgtg
     2881 tgcgtgtgcc cgagcgtctc tgtgtggatc agtgcacgtc tgtgtgcgtg tgcacgagcg
     2941 tctctgtgtg gatcagtgca cgtctgtgtg cgtgtgcacg agcgttctat gtggatcagt
     3001 gcacgcctgt gtgcgtgtgc acgagcgtct ctgtgtgtca gtgcacgtct gtgtgcgtgt
     3061 gcacaagcgt ctctgtgtgg atcagtgcac gtctgtgtgc gtgtgcacga gcgtctctgt
     3121 ggatcagtgc acgtctgtgt gcgtgtgcac gagcgttcta tgtggatcag tgcacgcctg
     3181 tgtgcgtgtg cacgagcgtc tctgtgtgtg tcagtgcacg tctgtgtgtg cacgagcgtc
     3241 tctgtgtgga tcagtgcacg tctgtgtgcg tgtgcccgag cgtctctgtg tggatcagtg
     3301 cacgtctgtg tgcgtgtgca caagcatctg tgtgtgtcag tgcacgtctg tgtgcgtgtg
     3361 cacgagcgtc tctggatcag tgcacgtctg tgcatgtgca cacgtgcatg tgcatgtgct
     3421 tacacgtctt tgtgtgtctg tgtgcgtgtg tccgcagttg ccctgtcctc gctgtcctcg
     3481 gtttggcaga caggtccctc accttccttg cctttcatct cctgtgaccc tcgccaggcc
     3541 ccttctctgt cttctgcgta cggcgtgttg cttcgtgaca gacggtgtga tcgcgggagc
     3601 tgtctcatgc ctcttgttct ccgggctgat tctgaaagag tggcttgcag gccaatcaat
     3661 gcaatcctgg caacctgccc gcccttcttg cgggcagagt tctttaccgt aagcgtcccc
     3721 agaccctcag gcctgcggaa acctgctggg ccttaccgtc tttgcttagc ttttggccta
     3781 gaccttaaag accagcagtc ttctgtgctt tatcctgctg cctccagagt gccatggaca
     3841 gatcacccat tctcgatcac tccggcagcc tttcggatcc ggtggcgccc ttcctggctg
     3901 tgtccttcac ctgtgctgtc tggttctctc ctgtgtggac aggggatgat aggaattccc
     3961 gctgcacagg ccgctgggag gacgggctca ggtgacatct gccgaggctt tggctcacca
     4021 gaggagtttg tagatgtcag tcatccccaa gtcactagga actagcacgc ctgaggtagg
     4081 tctcggaata gtgttcaaat ccattttact attgcggcca aggagccctc cctccagcaa
     4141 ggcggctcat cacatcacgt tactctttag gtcaaatgta accctcccgg ttttatttat
     4201 cactcaggca gagtttctta accttttgtg agttacagat cactgaaatt ctggtgaaag
     4261 ccaggtaggc ctcgccgcag gagaacacat acatggtgca cagaacacac acagtgcaca
     4321 gaacacagtg cacaggagaa cactcacagt gcacaggaga acacgcacac ggtgcacagg
     4381 agaacacgca cacatgttgc acaggagaat tcacacacag tgcacaggag aacacacagt
     4441 gcacaggaga acacatggtg cacaggagaa cacgcacaca cggtgcacag gagaacacac
     4501 acagtgcaca ggagaacacg cacagtgcac aggagaacac agagtgcaca ggagaacaca
     4561 tggtgcacag gagaacacgc acacacggtg cacagaacac acacagtgca caggagaaca
     4621 cgcacagtgc acaggagaac acggtgcacc ggagaacatg cacacatggt gcacaggaga
     4681 acacagtgca caggagaata cagactgcac agaacacacg cacagtgcac aggagaaaac
     4741 gcacacacag tgcacagaac acagtgcaca ggagaacacg cacacagtgc acagagaacc
     4801 acacagtgca caggagaata cgcacacaca gtgcacagaa cacagacaca gtacacagga
     4861 gaacacgcac acacagtgca cagaacacag ccagtgcaca ggagaacact gcacaggaga
     4921 accacactgc acaggagaat atgcataaac agttcacagg agaacacgca cacacagtgc
     4981 atagagcaca gacacagtgc acaggagaac cacacaatgt acaggagaat acgcacacac
     5041 agtgcacagg agaacataca cgcagtacac agacacagtg caccggagaa cacagtgcac
     5101 aggagaacac gcacacacag tgcacagaac acagacacag tgcacaggag aacacacagt
     5161 gcacaggaga acacagtgca caggagaata cacacggtgc acaggagaat tcacacacaa
     5221 tgcacaggag aacacagtgc acaggagaac acacacacac actgcacagg agaccatgca
     5281 cagtgcacag gagaatacac atggtgcaca gcagaattca cacagtgcac aggagaacac
     5341 acacagtgca cagaattcat gcacacagtg cacaggagaa cacacacagt gcacaggaga
     5401 acacagtgca caggagaaca cacactgcac agaacaaatg cacagcacac aggagaacat
     5461 gcacacacag tgcacaggag aacacagtgc acaggagaac acacagtgca caggagagaa
     5521 cacacagtgc acagaacaca cagggcacag gagaacacac agtgcacagg agaacactgc
     5581 acagaacaca cggtgatcag aacacacagt gcacaggaga acacacagtg cacagaacac
     5641 acggggcaca ggagaacaca cgatgcacgg aacacggtga tcagaacacg gttcacagga
     5701 gaacacacag tgcacagaac acacggggca caggagaaca cacagtgcac agaacacaca
     5761 gtgcacagaa cacacggtgc acaggagaac acatggtgca caggagaaca cacggtgtac
     5821 agaacacacg gtgcacagga gaacacgcag tgcacaggag aacatgcaat gcacgggaga
     5881 acacagtgca gagaacacac acgcactgca cagaacatgc agtggtgcac aggagaacag
     5941 tacacaacaa cacattccat gcacaggaga acacacgcaa ttcacaggac acaacagaac
     6001 acacgcagtg cacaggacac aacagaacac acgtgcacag gagaacacag tacacacaca
     6061 gcgcactgga gaacacgcac agtgcacagg agaacagagt gcacagaaca cagtccacag
     6121 gacagcacac ggtacacagc agaacacccg cagtgcacag gagaacctag tgcacagaac
     6181 acacaccctg tgcacagttt tgttttgttt tgcttttgca attccaggga gctccctggc
     6241 ctcccacagc cattcataga gcccattctg tttgcgataa aaacagatct cctccagctc
     6301 acgtgaggct aggcacgggt tcctcaaaac ctttttgggc agctagcagc tttgactagc
     6361 agctcatctc accaggctga ggtcgctgcc tgtgaacagt tgacagacct aaagccagcc
     6421 agaatgttct gtgtcagaga gccctgtcca atgggcagag ctgggagaca cgcaggaaga
     6481 cccccagccc atgcccgttc tcaggcctga ggttcattct cgttctctcc ctcttccctt
     6541 tccccttcct ccctcctgcc ctcgaatctg caaggtctct ccgggtggct gtgttctgcc
     6601 agggcctccc aggctccaag atcactgcct tcagggagcc caggaagagc tgcacatccg
     6661 ctgcctctgt gtggcaggcg tgtccgagac tgcaactcct agttcccacc tttcacacct
     6721 catctctcta acctcttatg gaaaatttca aacatatgta aaagcaggga gaaattgcat
     6781 aacgaaccta cagacctatc acccagcttc cacagtcatc agtggttttc tgtttttatc
     6841 aaactttatc tttgtacttc ttacagtgct tcacatttag caggtcctca atgaacatta
     6901 gatggatgga tggaaggaag gaagatggat ggatggatgg atggatggat ggatggatgg
     6961 atggatgaca gatggatggt ggagggttaa tggaaggaag gaagatggat gggtggaagg
     7021 aagatgggtg gatggatggt ggagggtgga tagaaggaag atgggtatat gaatagatgg
     7081 ctgggtgggt ggaaggaagg aagatggttg gatggatgga agatggatgg atggatggat
     7141 ggagggtaga gttttaatgg aaggaagatg gatggacgaa tagatggaag gaagatgggt
     7201 ggatggaagg aagatggatg gatggaatga tggatgggtg gtagagggtg gatggaagga
     7261 agatggatga atagatgggt gggtgggtgg atgtttggat ggatggtgga gggcagagtg
     7321 ttaatggaag gaagatgaat ggatagatgg agggtagagt gtagatggaa ggaaaggaaa
     7381 ggaggatgga tggatggatg gatggataga tggatggagg agggtggatg gaaggaagga
     7441 ggatggatgg atggatgggt gggtgggtgg gtggatggat ggcgcagggt atatggaagg
     7501 aagatggatg aatgatggaa gatggatgga tgggggacag atgaattgat ggcggaggat
     7561 gttaggatgg gtggatggat gtattaacat cccttctcca tgcctcagtc cccatcataa
     7621 ggagaaagag cctgtcgtcc ctctatgata ctgcactgaa ccaatattcc cctcacttca
     7681 tgggtgttac aaacctctta tcatctgttt acgtctcact cttactgtag aacctctaga
     7741 aaggcaggtg gcttccagct cctcgctttt cctgagggat gatctgacca cccattctta
     7801 cacatgtcat ctttcccaca gtggcagtgc agaggagaca aaagcagaac aaaagcagac
     7861 agcggccagg tctgagggtg ataggctact cactgggtcc tagtgttctg aactacaccc
     7921 tacaggaaag tgctactcag tgcccaggtg acatcgtcac aaggggaaca cagtggctaa
     7981 gagggcaggg gctcagtctc acagacctgg tttccatccc acttgctctc aggaaccctg
     8041 ggcaacaact gtgaccacag tgtcttcctc tggaaagtgc ccagcagtgt tgggctcacc
     8101 gtagctgcac aataaattgt catgagtgct ttccactgtg tcccaggttc aaggctgaag
     8161 tcacaacccc acccctgcca cagagcctgc ccccccgagt gttccccatc tcagtctgag
     8221 gcccgacctg tgcttccgga gcccgcgtcc cgtcccatct caggagctac tctgctccat
     8281 tccccccgta tccgagacct aagttccggc tctccttatc tcttggagcc ctgtcatctg
     8341 tggatcagat gctccccagc tccctccatc ccacccacag ggtttcacaa gaggcccagc
     8401 catgcaccct gctggggttc agacttgagg ggtgccactg gagttggggt gcacctgctc
     8461 tcacctgttc atgggctcct ttttcttctg aaatgcagcg tgaggcacca tggagggagc
     8521 tgtagacttt ggctccggcc agtgcccagt tcaaatgctg gccccaccac ttccagctgg
     8581 gtggcccagg gcaagccacc tacccttgct ttgcctcagt ttcctcatct ttaagtggca
     8641 gtaaccatag taccgacccc acagcctgct gagaggctca aaggagacga tgtatataaa
     8701 gcagttaact gatgcctgcc ctgggaagca gagcagtccc tgtcatgatg ggcgtttcta
     8761 catctggacc tgggcgccac catcctgctc acctgaatgc gggttctggg atctttcagt
     8821 ccttatttgg atatttgaat atttgttggt tttctggctt ctcacaattt ctaagaggtg
     8881 actaagtgga tcattcgttc ccgggagagg tgggaatggc tcctgtggac acggcattgc
     8941 acaggggctt gaggggacct cctgacccct gatggctgag cacccttgtc atactcctca
     9001 gggagagccc aggccaccgg ctagctccag actggctctg cagggcctca gaatctgaac
     9061 tccctgtggt cactctgagg cctctctgtc tctctgtgtc tttctctttc taccctctcc
     9121 atccctcctt ccctctgagg gcttgacctt cactgacctc cagggcaaga acaaccaaga
     9181 gttgctccca agagggcttc cctcctcctc gttctgtgtg gcctcagaag tgagggaatc
     9241 tcagtgctct gtgcaggcat gtgcttattc tgagctctgt ttgaaattaa acatgaggtg
     9301 tactaaagat ttaaatcgag caaacacctc tgtgatttcc aattggattc tgaggcgctt
     9361 acagaaatat gaagtccatt ttgatctact cttagaaaca aaagctgccc atgatttcac
     9421 cacagaaagt ctgcctcatt tgcagtttag cggactcaca gaggtgaccc tagatctctg
     9481 tctcgccttt gtttggttta tttccacatt gcatttgcgt ttcccgcctg gctagttctt
     9541 gatcattgag gaaggcagag agaaacgaga ttctctgctc tgggccaggc attctctgta
     9601 cacgttcctg gttccagcct gcctccttcc ctagaggatc accgtgtcca gccctgaagc
     9661 tggagcgagt gttcctcact tagagatgag gcgatgaggc ggcgagctgg gagagggctg
     9721 agcagctggt gagggcagag ttgggactca aacccaggcc cgaccaccac ccagctctcc
     9781 ttccaccgca ctggtccacc tcctgaccgt acgtggggag acccacgcaa aggcccagag
     9841 aggagaaggc cagcagtggg cagaacagag tgggccgcag gttttggcaa gcctggaggg
     9901 gaataggtca gaaaggtagc ccggctgggc tgagagactg aggacagggg gtgttagggt
     9961 gctgacattc tggcaagggg gcgggggtct gtggaagaca accccaggct ggggtggctt
    10021 ctctcaaacc cgggcatgca cagtgctggc cgtatcggtc cgagccctcg gctgccaggg
    10081 agagaagctg agctcccacc aaagcataaa atccaggcca gcgaggggca gccagaccac
    10141 gggaagacag gaggaacccg gacctggctc tcgtgcagga agcaggcagt gcacagctcc
    10201 gccgccacag agacagcctc ctctcatgac cccagactca gcccgggccg tggtgggaac
    10261 gatggttggc ccggtttgga gatgggggat ctggccctgt ggtgagtgca cccagagcag
    10321 gtgtctctcc ggagcagttg agtagaacag ctggcgtcac ctcttcatgt tgacgagggg
    10381 agatgagtgg cccaggcagt tcctagagcg ggggagatgg agtgggccgt atcccaccca
    10441 gcaggagcgc ttgcttctga agctcccgtg cgtctgtgac agaggcttga acccccggct
    10501 tgttctctca cgtctgttgc cgtaaatctg tctccatcgc ctggcacata ggagaggctt
    10561 ggcaaatatt tttggttcac tgaagaaagg cagccctcac tcctgctgct cagacagcac
    10621 cccctgaacg tgagttactg cctccttgag cactttcgca ggagagaaga ccagagagct
    10681 gccttcaaag atgccagagc catgtttatt taagcggcgc tgctccagag agaactggcc
    10741 atttgcagtc tccaccccag tgtggctcac gatgccacct ctccttgcca gctctcaccg
    10801 aggcccccgg tgacctccta gggggtacat ccagccaggt tccccctcag caatggccag
    10861 gggccaccgt gggccacact gcacttccca aatgctcccg gcactggcac agatgcacac
    10921 cctcttggcc atccccacct gtgctccgcc actcttagtc agccattcct ctcggcttcc
    10981 gggagtcccc tccttctcta aggatctcac ccccatccgt gtctctgctg tctcctaact
    11041 accgatagca agggcactag tgcccctgtt tgccggggac aggccctgtc tggggccctg
    11101 gtgtggcatc attgttaaca gagcttcttt tcactctgaa gtgttcagtt aggacagtca
    11161 cttgcacagt cccctcactg gtgacacaga caccccatac ctgtgtgttt cccctgcata
    11221 gctcagcagc agctcaggct cagcacggcc aaggctgaat gcagatgccc tccctccaaa
    11281 acctggcctc ctccagctgt tccttcctgg cgaggccacc tccaactgtg taacacagaa
    11341 gcctgggtgc catccctggg gctcccctca cccccacctc ccacccgtcc ccgggcccct
    11401 ggacagacca ctggccttgt tctcttcatt tccctggccc aggttacaaa gtctccttcc
    11461 tgattatgtg cattaactaa cctcgcaggt gctctgccca cctgcctacg ccagactctt
    11521 ccagatcctt ctccacccca gcaggcagag ttgtcattgt gaaatgcaaa tcaggttcca
    11581 tctctcctct cctttcctac ctcccaccat ccccgtcacc ctccctgtca cacacagccc
    11641 cctccgtggc ctctcaagcc tgctgtggcc tgaccctccc ctctgctttt ccaggccctc
    11701 ctggcactgt cctccctcag tctgggactc cctgtcctcc ctccttcctg cgtggcacat
    11761 gctcctccct cttcttgaaa cgctctcccc tccctgcctc ccaggcaatg cccactctgc
    11821 ctccaaagtt catccatcgt cctctcctca ggaagatttc cctggtggct tgactaggtc
    11881 acagtccccc tttatttgcc gtcttctcac tggggcctct ccttcctggt agcttctaaa
    11941 caaggcagtt tcatgtgtgt atctccacta gactagacgc tctctgaggg cagggaccct
    12001 gcttcgcttc tggcccgagg tggctgcagg gtctgcaaga tcatcagggc cccctccaat
    12061 gtgacacttg cagaggggac ccctggcctg gggccacgct ggttacttgg gtacactcct
    12121 tgcccagggt tccacagccc cttaaaggca gggtctttct ggccacgcct ggccctggtc
    12181 tggcctcccc aaggagccct gccgctgggg gtgctgtgtg ggaacagagc acaggccctc
    12241 cctcccagag cccacgagat ctcactttct ccaagcccag ctccccaagg actcagaccc
    12301 cagttgccct gcactgaacg ctggcttgcc ctagctcact cggaccccac cctgcactgg
    12361 gaaggagggt ccctgtcccc gtttgacggg tgacgacact gaggcccaga gagctgaagg
    12421 ggctggccga ggggacgggc ttgggcgctt ccattctcac actgctgccg tgacacctaa
    12481 tccatccggg gaagttccct ttaaacccaa aaggggacgg tgaagccaca cgcggccgcc
    12541 cttctcagga gcccacttcc acctctcgca ggctagacag cgttccccaa gtcccccctt
    12601 gagctgggtg ggctgcccag ctgtgaacac aaggacacct ccgatttcgt cctgtgaatg
    12661 ttgatattag tggtcccttc cttcaggggc tgtggggatc tcaaaagata aggcaggtct
    12721 ggagtcctgc gcggtacctg ccgcatggca ggctctttac ggacagcgca gggacgcact
    12781 ttgatgctga aaatgagtct ctgggacaga tggatcccag tctgggtcct cgcaagcgct
    12841 gtgcaccccg tcgccgcgag atggcggtgt tgcgcccctc cagcctcctc tgctctgcgc
    12901 ttgggcgctg aatgttaaaa ttcctaacag gtgaatcgct tgaatccggg aggcggaggt
    12961 tgcagttggc cgagatggcg ccactcactc cagcctggcg acagagcgag actacgtctc
    13021 aaaaaaaaat aaaaataaaa aaataaaatc ctaa
//
