LOCUS       NM_022372               1671 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens MTOR associated protein, LST8 homolog (MLST8),
            transcript variant 1, mRNA.
ACCESSION   NM_022372
VERSION     NM_022372.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Yu,Z., Chen,J., Takagi,E., Wang,F., Saha,B., Liu,X., Joubert,L.M.,
            Gleason,C.E., Jin,M., Li,C., Nowotny,C., Agard,D., Cheng,Y. and
            Pearce,D.
  TITLE     Interactions between mTORC2 core subunits Rictor and mSin1 dictate
            selective and context-dependent phosphorylation of substrate
            kinases SGK1 and Akt
  JOURNAL   J Biol Chem 298 (9), 102288 (2022)
   PUBMED   35926713
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Zhang,L. and Su,X.
  TITLE     Bioactive peptide inhibits acute myeloid leukemia cell
            proliferation by downregulating ALKBH5-mediated m6A demethylation
            of EIF4EBP1 and MLST8 mRNA
  JOURNAL   Cell Oncol (Dordr) 45 (3), 355-365 (2022)
   PUBMED   35579750
  REMARK    GeneRIF: Bioactive peptide inhibits acute myeloid leukemia cell
            proliferation by downregulating ALKBH5-mediated m(6)A demethylation
            of EIF4EBP1 and MLST8 mRNA.
REFERENCE   3  (bases 1 to 1671)
  AUTHORS   Meng,D., Yang,Q., Jeong,M.H., Curukovic,A., Tiwary,S., Melick,C.H.,
            Lama-Sherpa,T.D., Wang,H., Huerta-Rosario,M., Urquhart,G.,
            Zacharias,L.G., Lewis,C., DeBerardinis,R.J. and Jewell,J.L.
  TITLE     SNAT7 regulates mTORC1 via macropinocytosis
  JOURNAL   Proc Natl Acad Sci U S A 119 (20), e2123261119 (2022)
   PUBMED   35561222
REFERENCE   4  (bases 1 to 1671)
  AUTHORS   Zhang,E., Chen,S., Tang,H., Fei,C., Yuan,Z., Mu,X., Qin,Y., Liu,H.,
            Fan,Y., Tan,M. and Wang,X.
  TITLE     CDK1/FBXW7 facilitates degradation and ubiquitination of MLST8 to
            inhibit progression of renal cell carcinoma
  JOURNAL   Cancer Sci 113 (1), 91-108 (2022)
   PUBMED   34741373
  REMARK    GeneRIF: CDK1/FBXW7 facilitates degradation and ubiquitination of
            MLST8 to inhibit progression of renal cell carcinoma.
REFERENCE   5  (bases 1 to 1671)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 1671)
  AUTHORS   Jacinto,E., Loewith,R., Schmidt,A., Lin,S., Ruegg,M.A., Hall,A. and
            Hall,M.N.
  TITLE     Mammalian TOR complex 2 controls the actin cytoskeleton and is
            rapamycin insensitive
  JOURNAL   Nat Cell Biol 6 (11), 1122-1128 (2004)
   PUBMED   15467718
REFERENCE   7  (bases 1 to 1671)
  AUTHORS   Sarbassov,D.D., Ali,S.M., Kim,D.H., Guertin,D.A., Latek,R.R.,
            Erdjument-Bromage,H., Tempst,P. and Sabatini,D.M.
  TITLE     Rictor, a novel binding partner of mTOR, defines a
            rapamycin-insensitive and raptor-independent pathway that regulates
            the cytoskeleton
  JOURNAL   Curr Biol 14 (14), 1296-1302 (2004)
   PUBMED   15268862
REFERENCE   8  (bases 1 to 1671)
  AUTHORS   Kim,D.H., Sarbassov,D.D., Ali,S.M., Latek,R.R., Guntur,K.V.,
            Erdjument-Bromage,H., Tempst,P. and Sabatini,D.M.
  TITLE     GbetaL, a positive regulator of the rapamycin-sensitive pathway
            required for the nutrient-sensitive interaction between raptor and
            mTOR
  JOURNAL   Mol Cell 11 (4), 895-904 (2003)
   PUBMED   12718876
REFERENCE   9  (bases 1 to 1671)
  AUTHORS   Loewith,R., Jacinto,E., Wullschleger,S., Lorberg,A., Crespo,J.L.,
            Bonenfant,D., Oppliger,W., Jenoe,P. and Hall,M.N.
  TITLE     Two TOR complexes, only one of which is rapamycin sensitive, have
            distinct roles in cell growth control
  JOURNAL   Mol Cell 10 (3), 457-468 (2002)
   PUBMED   12408816
REFERENCE   10 (bases 1 to 1671)
  AUTHORS   Rodgers,B.D., Levine,M.A., Bernier,M. and Montrose-Rafizadeh,C.
  TITLE     Insulin regulation of a novel WD-40 repeat protein in adipocytes
  JOURNAL   J Endocrinol 168 (2), 325-332 (2001)
   PUBMED   11182770
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC009065.8 and BC001313.2.
            
            On Nov 23, 2018 this sequence version replaced NM_022372.5.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.363454.1, BC088354.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000569417.6/ ENSP00000456405.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                AC009065.8         71926-71958         c
            34-1615             BC001313.2         1-1582
            1616-1671           AC009065.8         67959-68014         c
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..1671
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /note="MTOR associated protein, LST8 homolog"
                     /db_xref="GeneID:64223"
                     /db_xref="HGNC:HGNC:24825"
                     /db_xref="MIM:612190"
     exon            1..59
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            60..243
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..1095
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /note="isoform a is encoded by transcript variant 1;
                     target of rapamycin complex subunit LST8; gable; protein
                     GbetaL; TORC subunit LST8; mammalian lethal with SEC13
                     protein 8"
                     /codon_start=1
                     /product="target of rapamycin complex subunit LST8 isoform
                     a"
                     /protein_id="NP_071767.3"
                     /db_xref="CCDS:CCDS10462.2"
                     /db_xref="GeneID:64223"
                     /db_xref="HGNC:HGNC:24825"
                     /db_xref="MIM:612190"
                     /translation="MNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQ
                     VNALEVTPDRSMIAAAGYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMYT
                     GGEDCTARIWDLRSRNLQCQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTD
                     HNEQLIPEPEVSITSAHIDPDASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPA
                     HTRYALQCRFSPDSTLLATCSADQTCKIWRTSNFSLMTELSIKSGNPGESSRGWMWGC
                     AFSGDSQYIVTASSDNLARLWCVETGEIKREYGGHQKAVVCLAFNDSVLG"
     exon            244..295
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            296..458
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            459..534
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            535..687
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            688..812
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            813..976
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1671
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1594..1599
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /note="hexamer: AATAAA"
     polyA_site      1615
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /note="major polyA site"
     regulatory      1648..1653
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
                     /note="hexamer: GATAAA"
     polyA_site      1653
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
     polyA_site      1671
                     /gene="MLST8"
                     /gene_synonym="GbetaL; GBL; LST8; POP3; WAT1"
ORIGIN      
        1 gactcgctcc ggctcccctc gccgtcctgg acacggcgga gtgcggagcc gcccgtaaga
       61 tgctctgacc tttgacccct gccgttcagc tctagggccc gtgcaggcca caccatgaac
      121 acctccccag gcacggtggg cagtgacccg gtcatcctgg ccactgcagg ctacgaccac
      181 accgtgcgct tctggcaggc ccacagcggc atctgcaccc ggacggtgca gcaccaggac
      241 tcccaggtga atgccttgga ggtcacaccg gaccgcagca tgattgctgc tgcaggttac
      301 cagcacatcc gcatgtatga tctcaactcc aataacccta accccatcat cagctacgac
      361 ggcgtcaaca agaacatcgc gtctgtgggc ttccacgaag acggccgctg gatgtacacg
      421 ggcggcgagg actgcacagc caggatctgg gacctcaggt cccggaacct gcagtgccag
      481 cggatcttcc aggtgaacgc acccattaac tgcgtgtgcc tgcaccccaa ccaggcagag
      541 ctcatcgtgg gtgaccagag cggggctatc cacatctggg acttgaaaac agaccacaac
      601 gagcagctga tccctgagcc cgaggtctcc atcacgtccg cccacatcga tcccgacgcc
      661 agctacatgg cagctgtcaa tagcaccgga aactgctatg tctggaatct gacggggggc
      721 attggtgacg aggtgaccca gctcatcccc aagactaaga tccctgccca cacgcgctac
      781 gccctgcagt gtcgcttcag ccccgactcc acgctcctcg ccacctgctc ggctgatcag
      841 acgtgcaaga tctggaggac gtccaacttc tccctgatga cggagctgag catcaagagc
      901 ggcaaccccg gggagtcctc ccgcggctgg atgtggggct gcgccttctc gggggactcc
      961 cagtacatcg tcactgcttc ctcggacaac ctggcccggc tctggtgtgt ggagactgga
     1021 gagatcaaga gagagtatgg cggccaccag aaggctgttg tctgcctggc cttcaatgac
     1081 agtgtgctgg gctagcctgt gacccctcgg gactgcctgg tgcaggtggt ggcagctgga
     1141 gggacccatg cagcacccag gtcagagcag accctcccct gccggcctgc gccagctgga
     1201 cctgatggcc ccctgtggcg ccttgacctg ctgggccagg ctgccctggg actctcagcc
     1261 cccagttgct tatccagatg tgacagagct cgacccaagc caggctgcac actcctggac
     1321 tgggctagcc tgcactgcct gggaaagtcg gccgagggcc caaagctgct gaggggtctg
     1381 aggctggtgc ccacccccaa gctagtgtgt tctctgcccc tccctgcccg cgtttcaggg
     1441 cctcggtcca tagagaacac caccaccatg gccaggtgga agggtttatt agtccctgcc
     1501 agcagctgtc ctccctggtg caggtggcct ggccagccca ctggattggg gacgggccag
     1561 gctgggccag gtcgggggct cagtctggga ggtaataaaa gcagaccgac acgcagatgt
     1621 tgctcgggaa gcagatgtcg atgcagagat aaatcagccg ctgtctccgg g
//
