LOCUS       NM_173576               3609 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens mohawk homeobox (MKX), transcript variant 1, mRNA.
ACCESSION   NM_173576
VERSION     NM_173576.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdul Majeed,S., Dunzendorfer,H., Weiner,J., Heiker,J.T., Kiess,W.,
            Korner,A. and Landgraf,K.
  TITLE     COBL, MKX and MYOC Are Potential Regulators of Brown Adipose Tissue
            Development Associated with Obesity-Related Metabolic Dysfunction
            in Children
  JOURNAL   Int J Mol Sci 24 (4), 3085 (2023)
   PUBMED   36834493
  REMARK    GeneRIF: COBL, MKX and MYOC Are Potential Regulators of Brown
            Adipose Tissue Development Associated with Obesity-Related
            Metabolic Dysfunction in Children.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Lee,K.I., Gamini,R., Olmer,M., Ikuta,Y., Hasei,J., Baek,J.,
            Alvarez-Garcia,O., Grogan,S.P., D'Lima,D.D., Asahara,H., Su,A.I.
            and Lotz,M.K.
  TITLE     Mohawk is a transcription factor that promotes meniscus cell
            phenotype and tissue repair and reduces osteoarthritis severity
  JOURNAL   Sci Transl Med 12 (567) (2020)
   PUBMED   33115953
  REMARK    GeneRIF: Mohawk is a transcription factor that promotes meniscus
            cell phenotype and tissue repair and reduces osteoarthritis
            severity.
REFERENCE   3  (bases 1 to 3609)
  AUTHORS   Nakamichi,R., Ito,Y., Inui,M., Onizuka,N., Kayama,T., Kataoka,K.,
            Suzuki,H., Mori,M., Inagawa,M., Ichinose,S., Lotz,M.K., Sakai,D.,
            Masuda,K., Ozaki,T. and Asahara,H.
  TITLE     Mohawk promotes the maintenance and regeneration of the outer
            annulus fibrosus of intervertebral discs
  JOURNAL   Nat Commun 7, 12503 (2016)
   PUBMED   27527664
  REMARK    GeneRIF: Data show that homeobox protein Mohawk (Mkx) is mainly
            expressed in the outer annulus fibrosus (OAF) of humans and mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3609)
  AUTHORS   Liu,H., Zhang,C., Zhu,S., Lu,P., Zhu,T., Gong,X., Zhang,Z., Hu,J.,
            Yin,Z., Heng,B.C., Chen,X. and Ouyang,H.W.
  TITLE     Mohawk promotes the tenogenesis of mesenchymal stem cells through
            activation of the TGFbeta signaling pathway
  JOURNAL   Stem Cells 33 (2), 443-455 (2015)
   PUBMED   25332192
  REMARK    GeneRIF: these data shed light on previously unrecognized roles of
            Mkx in tendinopathy, tenogenesis, and tendon repair as well as in
            regulating the TGFbeta pathway.
REFERENCE   5  (bases 1 to 3609)
  AUTHORS   Otabe,K., Nakahara,H., Hasegawa,A., Matsukawa,T., Ayabe,F.,
            Onizuka,N., Inui,M., Takada,S., Ito,Y., Sekiya,I., Muneta,T.,
            Lotz,M. and Asahara,H.
  TITLE     Transcription factor Mohawk controls tenogenic differentiation of
            bone marrow mesenchymal stem cells in vitro and in vivo
  JOURNAL   J Orthop Res 33 (1), 1-8 (2015)
   PUBMED   25312837
  REMARK    GeneRIF: Transcription factor Mohawk controls tenogenic
            differentiation of bone marrow mesenchymal stem cells in vitro and
            in vivo.
REFERENCE   6  (bases 1 to 3609)
  AUTHORS   Ito,Y., Toriuchi,N., Yoshitaka,T., Ueno-Kudoh,H., Sato,T.,
            Yokoyama,S., Nishida,K., Akimoto,T., Takahashi,M., Miyaki,S. and
            Asahara,H.
  TITLE     The Mohawk homeobox gene is a critical regulator of tendon
            differentiation
  JOURNAL   Proc Natl Acad Sci U S A 107 (23), 10538-10542 (2010)
   PUBMED   20498044
REFERENCE   7  (bases 1 to 3609)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   8  (bases 1 to 3609)
  AUTHORS   Anderson,D.M., Arredondo,J., Hahn,K., Valente,G., Martin,J.F.,
            Wilson-Rawls,J. and Rawls,A.
  TITLE     Mohawk is a novel homeobox gene expressed in the developing mouse
            embryo
  JOURNAL   Dev Dyn 235 (3), 792-801 (2006)
   PUBMED   16408284
REFERENCE   9  (bases 1 to 3609)
  AUTHORS   Weinmann,A., Galle,P.R. and Teufel,A.
  TITLE     In silico characterization of an Iroquois family-related
            homeodomain protein
  JOURNAL   Int J Mol Med 16 (3), 443-448 (2005)
   PUBMED   16077953
  REMARK    GeneRIF: In silico characterization of the C10orf48 sequence
REFERENCE   10 (bases 1 to 3609)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC024606.11, DB285243.1,
            BC036207.1 and AW020078.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_173576.2.
            
            Summary: The protein encoded by this gene is an IRX family-related
            homeobox protein that may play a role in cell adhesion. Studies in
            mice suggest that this protein may be a regulator of tendon
            development. Two transcript variants encoding the same protein have
            been found for this gene.[provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK125339.1, SRR1660809.63160.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467144 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000419761.6/ ENSP00000400896.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-113               AC024606.11        139579-139691       c
            114-383             DB285243.1         49-318
            384-1033            BC036207.1         380-1029
            1034-1067           AC024606.11        69393-69426         c
            1068-3322           AC024606.11        67033-69287         c
            3323-3609           AW020078.1         8-294
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.1"
     gene            1..3609
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /note="mohawk homeobox"
                     /db_xref="GeneID:283078"
                     /db_xref="HGNC:HGNC:23729"
                     /db_xref="MIM:601332"
     exon            1..113
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /inference="alignment:Splign:2.1.0"
     exon            114..383
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             196..1254
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /note="iroquois homeobox protein-like 1; Iroquois family
                     related homeodomain protein"
                     /codon_start=1
                     /product="homeobox protein Mohawk"
                     /protein_id="NP_775847.2"
                     /db_xref="CCDS:CCDS7156.1"
                     /db_xref="GeneID:283078"
                     /db_xref="HGNC:HGNC:23729"
                     /db_xref="MIM:601332"
                     /translation="MNTIVFNKLSGAVLFEDGGASERERGGRPYSGVLDSPHARPEVG
                     IPDGPPLKDNLGLRHRRTGARQNGGKVRHKRQALQDMARPLKQWLYKHRDNPYPTKTE
                     KILLALGSQMTLVQVSNWFANARRRLKNTVRQPDLSWALRIKLYNKYVQGNAERLSVS
                     SDDSCSEDGENPPRTHMNEGGYNTPVHHPVIKSENSVIKAGVRPESRASEDYVAPPKY
                     KSSLLNRYLNDSLRHVMATNTTMMGKTRQRNHSGSFSSNEFEEELVSPSSSETEGNFV
                     YRTDTLENGSNKGESAANRKGPSKDDTYWKEINAAMALTNLAQGKDKLQGTTSCIIQK
                     SSHIAEVKTVKVPLVQQF"
     exon            384..543
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /inference="alignment:Splign:2.1.0"
     exon            544..697
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /inference="alignment:Splign:2.1.0"
     exon            698..1033
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1067
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..3609
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3584..3589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /note="hexamer: AATAAA"
     polyA_site      3609
                     /gene="MKX"
                     /gene_synonym="C10orf48; IFRX; IRXL1"
                     /note="major polyA site"
ORIGIN      
        1 ggtgagcgcg ctggagcccg cgtggagaac atgcggcggg gatgggagtg cgcctagtct
       61 cgaggcggga gagccagccg ccctgcagcc ggccgtcggc cccgcagcca cagaagccga
      121 gccccgctgc ggagctcccg gcggcccagc cccgaggctc tgcggccgcg ccgcgcgtcc
      181 ctaccaaccg acaccatgaa caccatcgtc ttcaacaagc tcagcggtgc ggtgctgttt
      241 gaggacggag gcgcctcgga gcgggagcgg ggtggccggc cctacagcgg tgtcctggac
      301 agtcctcacg cccgccccga ggtgggcatt cccgacggcc cgcccctcaa ggacaacctc
      361 ggcctgagac accggaggac cggcgcccgg cagaatggcg ggaaggtgag gcacaagcgg
      421 caggccctgc aagacatggc gcgacccctc aagcagtggc tttacaagca ccgtgacaac
      481 ccgtacccca ccaagaccga gaagatactc ttggccctcg gctcgcagat gacgctagtg
      541 caggtgtcaa attggtttgc taatgcaaga cgtcggctta agaataccgt tcgacagcca
      601 gatttaagct gggctttgag aataaagtta tacaacaagt atgttcaagg caatgctgaa
      661 cggcttagcg taagcagtga tgactcatgt tctgaagatg gagaaaatcc tccaagaacc
      721 cacatgaacg aagggggcta taatacccca gttcaccatc ctgtgattaa aagtgagaat
      781 tcggtcatca aagcgggagt gaggccagag tcacgggcca gtgaggacta cgtggcaccc
      841 cccaaataca agagcagctt gttgaaccgt taccttaatg actctttgag acatgtcatg
      901 gccacgaaca ctaccatgat gggaaaaaca aggcaaagaa accactcggg atcttttagc
      961 tccaatgaat ttgaggaaga attagtgtct ccatcgtcat cagaaactga aggcaacttt
     1021 gtctatcgca cagacactct ggaaaacgga tccaataagg gtgaaagcgc agctaacaga
     1081 aaaggaccaa gcaaggatga cacgtattgg aaggagatca acgcagctat ggccttaaca
     1141 aatcttgcac agggaaagga caaactgcag ggaactacca gctgcatcat ccagaagtcg
     1201 tcccatatag cagaagtaaa gactgtcaaa gtgccgctgg tgcagcagtt ttaagagctt
     1261 gttgcttttc agatccaatg gatgttcttt ccggtgtttt catcaaccct catcctaaga
     1321 gccgaagcag ggatgaaaat gactctctcc caaacctctt cttattttta attatcccaa
     1381 atatatcatt tagttgcttc tataaaagac atataaatta taaaaaactc attttaatca
     1441 aaaatattaa cttattttat gttactcaaa ctatgcataa aacatctgca ttaccattac
     1501 agtaagtgcc ttgcttcccg acaataagct ccaacgtggg catagttgaa caagctatgc
     1561 ctcaaaatgc caacgccata tgcttattag cctgtgtgca tcattccaga cgggcctaat
     1621 cattccagga ctgaaaccag aatcgctgaa agcccttgaa atacattcaa taattcatat
     1681 gttaaaactt ggatatctgt tcagcccaaa tgaaatcttc cttttaaaaa acgtctacat
     1741 tattgaaaat tgttcaatgt gcttttcaga gtgacggtga gaattttatg catgtatctt
     1801 gcctgcatat ttgatatgtt acaaacttcc aaaattcaag gtgcagcgat ccacagaacg
     1861 ttgtacattt aagaagtgat tccttcaagc taatttaaaa tttcattgaa cacatggtga
     1921 ccaggaaaac tttttttcaa gcactgttgg aaagcaccac aaagcccttt agaattaatc
     1981 tggatttgtt tctcaagttc tgctgaagtt taaaaaaaaa ctttattata caaataactc
     2041 aaaattttcc tgtgtaaaac taaacctgta gttttaaaac ataatcctgt ttgcattaga
     2101 gctcactgtc tttttgtgat ggaaactgtg ttcgtatgga atgactaaaa atcttttatt
     2161 tggtttgttt caaattacaa ttgctgatgg acaatttgta ttgcagcgag aacaacagaa
     2221 tgaaagaaat gtatctctgt gcggctatac atatacatac ataaaattga tttttaaatt
     2281 taaaacatat ggaaaacaaa acattgaaca gtttgaattt tgccaagttg gacattaaag
     2341 taaaaatgaa gtgaaatcat gcattgaaag aaaacatttt gtttctaaat tagtctacca
     2401 ttgagtgaga ataatcaata tcaagaaaga agactatctt tctcaactaa acaataatat
     2461 tccaatcagc ttgggaagac ctgaaacttg aataagcagt ggaaatgcca aatataacag
     2521 agggtatgtg ctacagagaa gtaaaaaggg tttgactttt tatgatggga tttttttttt
     2581 ctgggtatgt aatctatttt ttttttaaac tggaaagcat ttttgtcagt gtgaatgagg
     2641 gtcaatagtg cagccagtgg tgacattttt ctttattttg caaaatgctt ttaaaaccaa
     2701 aggctgctct agttgatgga cagtatcagt cttgatctaa attgtaggac actttttcat
     2761 gtaacataac atttggggat tgggtttatt tagtgtaatg aagataattt gatataaaaa
     2821 tattttgtgt atatatatat atttttactt tgttttctaa attgctgttt gcagtaacag
     2881 taagcgcaaa gcaaaatata taagttatga ctgtatgatc agatgaagta tgagttcttt
     2941 tggtttgcat ccttaaatag ttagagatct ctgataaaaa ctttggaatc tttgcaaaac
     3001 aatacaaaaa tgccaaaatg tgagcatgtc aatgaaaact aaagacaaat acttcactct
     3061 ttttcatact attataagtt attctggtat taaatatgtt aataaaagtg tttttgtttt
     3121 gacatatttc agttaaatga atgaatgctg gttgtatttt atttgaatga gtcatgattc
     3181 atgtttgcca tctttttaaa aaaatcagca aatttcttct atgttataaa ttatagatga
     3241 caaggcaata taggacaact attcacatga ttttttttaa taccaaaggt tggaagattt
     3301 tataattaac atgtcaagaa gactttatag taagcacatc cttggtaata tctccaattg
     3361 caatgacttt ttaatttatt ttttcttttg ctgctttaac attttctgga tattaaaatc
     3421 cccccagtcc tttaaaagaa tcttgaacaa tgctgagccg gcagctgaaa atctaactca
     3481 taatttatgt tgtagagaaa tagaattacc tctattcttt gttttgccat atgtaatcat
     3541 tttaataaaa ttaataactg ccaggagttc ttgacagatt taaaataaaa gttaatttct
     3601 agacctcga
//
