LOCUS       NM_001195279            1124 bp    mRNA    linear   PRI 16-FEB-2024
DEFINITION  Homo sapiens MKRN2 opposite strand (MKRN2OS), transcript variant 1,
            mRNA.
ACCESSION   NM_001195279 XM_001716334 XM_001717393 XM_001718351
VERSION     NM_001195279.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Muzny,D.M., Scherer,S.E., Kaul,R., Wang,J., Yu,J., Sudbrak,R.,
            Buhay,C.J., Chen,R., Cree,A., Ding,Y., Dugan-Rocha,S., Gill,R.,
            Gunaratne,P., Harris,R.A., Hawes,A.C., Hernandez,J., Hodgson,A.V.,
            Hume,J., Jackson,A., Khan,Z.M., Kovar-Smith,C., Lewis,L.R.,
            Lozado,R.J., Metzker,M.L., Milosavljevic,A., Miner,G.R.,
            Morgan,M.B., Nazareth,L.V., Scott,G., Sodergren,E., Song,X.Z.,
            Steffen,D., Wei,S., Wheeler,D.A., Wright,M.W., Worley,K.C.,
            Yuan,Y., Zhang,Z., Adams,C.Q., Ansari-Lari,M.A., Ayele,M.,
            Brown,M.J., Chen,G., Chen,Z., Clendenning,J.,
            Clerc-Blankenburg,K.P., Chen,R., Chen,Z., Davis,C., Delgado,O.,
            Dinh,H.H., Dong,W., Draper,H., Ernst,S., Fu,G.,
            Gonzalez-Garay,M.L., Garcia,D.K., Gillett,W., Gu,J., Hao,B.,
            Haugen,E., Havlak,P., He,X., Hennig,S., Hu,S., Huang,W.,
            Jackson,L.R., Jacob,L.S., Kelly,S.H., Kube,M., Levy,R., Li,Z.,
            Liu,B., Liu,J., Liu,W., Lu,J., Maheshwari,M., Nguyen,B.V.,
            Okwuonu,G.O., Palmeiri,A., Pasternak,S., Perez,L.M., Phelps,K.A.,
            Plopper,F.J., Qiang,B., Raymond,C., Rodriguez,R.,
            Saenphimmachak,C., Santibanez,J., Shen,H., Shen,Y., Subramanian,S.,
            Tabor,P.E., Verduzco,D., Waldron,L., Wang,J., Wang,J., Wang,Q.,
            Williams,G.A., Wong,G.K., Yao,Z., Zhang,J., Zhang,X., Zhao,G.,
            Zhou,J., Zhou,Y., Nelson,D., Lehrach,H., Reinhardt,R., Naylor,S.L.,
            Yang,H., Olson,M., Weinstock,G. and Gibbs,R.A.
  TITLE     The DNA sequence, annotation and analysis of human chromosome 3
  JOURNAL   Nature 440 (7088), 1194-1198 (2006)
   PUBMED   16641997
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC018500.4.
            
            On Jan 23, 2020 this sequence version replaced NM_001195279.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript and protein alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.1133859.1,
                                           DRR138506.59674.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000564146.4/ ENSP00000455385.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-253               AC018500.4         87010-87262         c
            254-303             AC018500.4         84943-84992         c
            304-466             AC018500.4         83623-83785         c
            467-1124            AC018500.4         81539-82196         c
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.2"
     gene            1..1124
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
                     /note="MKRN2 opposite strand"
                     /db_xref="GeneID:100129480"
                     /db_xref="HGNC:HGNC:40375"
     exon            1..253
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..707
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
                     /note="isoform 1 is encoded by transcript variant 1; MKRN2
                     antisense gene protein 1; MKRN2 opposite strand protein;
                     MKRN2 antisense RNA 1 (non-protein coding)"
                     /codon_start=1
                     /product="MKRN2 opposite strand protein isoform 1"
                     /protein_id="NP_001182208.1"
                     /db_xref="CCDS:CCDS58816.1"
                     /db_xref="GeneID:100129480"
                     /db_xref="HGNC:HGNC:40375"
                     /translation="MHCAEAGKALIKFNHCEKYIYSFSVPQCCPLCQQDLGSRKLEDA
                     PVSIANPFTNGHQEKCSFLLRPTQGTFLREYDGRSDLHVGITNTNGVVYNYSAHGVQR
                     DGEGWEESISIPLLQPNMYGMMEQWDKYLEDFSTSGAWLPHRYEDNHHNCYSYALTFI
                     NCVLMAEGRQQLDKGEFTEKYVVPRTRLASKFITLYRAIREHGFYVTDCPQQQAQPPE
                     GGGLC"
     exon            254..303
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
                     /inference="alignment:Splign:2.1.0"
     exon            304..466
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
                     /inference="alignment:Splign:2.1.0"
     exon            467..1124
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      800..805
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
                     /note="hexamer: AATAAA"
     polyA_site      822
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
     regulatory      1103..1108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
                     /note="hexamer: AATAAA"
     polyA_site      1124
                     /gene="MKRN2OS"
                     /gene_synonym="C3orf83; MKRN2-AS1"
                     /note="major polyA site"
ORIGIN      
        1 ggaaacctcc cctagcattc caggaggcga aagctatgca ctgcgcagag gctgggaagg
       61 ctttaattaa attcaaccac tgtgagaaat acatctacag cttcagtgtg ccccagtgct
      121 gccctctctg ccagcaggac ctgggctcga ggaagctgga ggacgcacct gttagcatcg
      181 ctaatccatt tactaatgga catcaagaaa aatgttcatt cctcctcaga ccaactcagg
      241 ggacatttct tagagagtat gatggaaggt ctgatcttca tgttggaata actaacacaa
      301 atggggttgt gtataattac agtgcacatg gtgtccagcg agacggagaa gggtgggaag
      361 agagcataag catcccatta ctgcagccca acatgtatgg aatgatggag caatgggaca
      421 agtacctgga agacttctcc acctcggggg cctggctgcc tcacaggtat gaagacaacc
      481 accataactg ctactcttac gcactcacgt tcattaactg cgttctgatg gcagaaggta
      541 gacagcaact ggacaagggt gaatttacgg agaagtacgt ggtcccgcgg acaaggctgg
      601 catccaagtt catcacactc taccgggcga tacgggagca tggcttctac gtcactgact
      661 gtccccagca gcaggcacaa ccccctgagg gcggcggttt gtgctgagag ctatgtaagc
      721 gcagcctgga cgctggaggg tagggtggtt gctaccttta atcagtacta tggatttcta
      781 aatgcattta actgtggtta ataaaagcgt gtatgggccg ggcatggtgg ctcacacctg
      841 taatcccagc actttgggaa gctaagacag gtaggtcacc tgaggttggg agtttgagac
      901 cagcctgacc aacatggaga aaccccgtcc ttactaaaaa tataaaatta gctgggcatg
      961 gtggcgcatg cctgtaatcc caactactag ggaggctgaa gcaggagaat cgcttgaacc
     1021 cgggaggcgg aggttgggat gagttgagat cgtgccattg cactccagcc tgggcaacaa
     1081 gagtgaaact ccatctcaaa aaaataaaaa ataaaaaata aaaa
//
