LOCUS       NM_014160               2775 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens makorin ring finger protein 2 (MKRN2), transcript
            variant 1, mRNA.
ACCESSION   NM_014160
VERSION     NM_014160.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Bonazza,S., Coutts,H.L., Sukumar,S., Turkington,H.L. and
            Courtney,D.G.
  TITLE     Identifying cellular RNA-binding proteins during infection uncovers
            a role for MKRN2 in influenza mRNA trafficking
  JOURNAL   PLoS Pathog 20 (5), e1012231 (2024)
   PUBMED   38753876
  REMARK    GeneRIF: Identifying cellular RNA-binding proteins during infection
            uncovers a role for MKRN2 in influenza mRNA trafficking.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Jiang,F.Z., Xia,Q.J., Wu,L. and Zhang,Y.M.
  TITLE     [Overexpression of MKRN2 Inhibits the Growth of Ovarian Cancer
            Cells]
  JOURNAL   Mol Biol (Mosk) 57 (4), 687-688 (2023)
   PUBMED   37528788
  REMARK    GeneRIF: [Overexpression of MKRN2 Inhibits the Growth of Ovarian
            Cancer Cells].
REFERENCE   3  (bases 1 to 2775)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   4  (bases 1 to 2775)
  AUTHORS   Yang,Y., Luo,Y., Huang,S., Tao,Y., Li,C. and Wang,C.
  TITLE     MKRN1/2 serve as tumor suppressors in renal clear cell carcinoma by
            regulating the expression of p53
  JOURNAL   Cancer Biomark 36 (4), 267-278 (2023)
   PUBMED   36938725
  REMARK    GeneRIF: MKRN1/2 serve as tumor suppressors in renal clear cell
            carcinoma by regulating the expression of p53.
REFERENCE   5  (bases 1 to 2775)
  AUTHORS   Li,J., Guo,M., Tian,X., Wang,X., Yang,X., Wu,P., Liu,C., Xiao,Z.,
            Qu,Y., Yin,Y., Wang,C., Zhang,Y., Zhu,Z., Liu,Z., Peng,C., Zhu,T.
            and Liang,Q.
  TITLE     Virus-Host Interactome and Proteomic Survey Reveal Potential
            Virulence Factors Influencing SARS-CoV-2 Pathogenesis
  JOURNAL   Med 2 (1), 99-112 (2021)
   PUBMED   32838362
REFERENCE   6  (bases 1 to 2775)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   7  (bases 1 to 2775)
  AUTHORS   Pfefferle,S., Schopf,J., Kogl,M., Friedel,C.C., Muller,M.A.,
            Carbajo-Lozoya,J., Stellberger,T., von Dall'Armi,E., Herzog,P.,
            Kallies,S., Niemeyer,D., Ditt,V., Kuri,T., Zust,R., Pumpor,K.,
            Hilgenfeld,R., Schwarz,F., Zimmer,R., Steffen,I., Weber,F.,
            Thiel,V., Herrler,G., Thiel,H.J., Schwegmann-Wessels,C.,
            Pohlmann,S., Haas,J., Drosten,C. and von Brunn,A.
  TITLE     The SARS-coronavirus-host interactome: identification of
            cyclophilins as target for pan-coronavirus inhibitors
  JOURNAL   PLoS Pathog 7 (10), e1002331 (2011)
   PUBMED   22046132
REFERENCE   8  (bases 1 to 2775)
  AUTHORS   Bohne,A., Darras,A., D'Cotta,H., Baroiller,J.F., Galiana-Arnoux,D.
            and Volff,J.N.
  TITLE     The vertebrate makorin ubiquitin ligase gene family has been shaped
            by large-scale duplication and retroposition from an ancestral
            gonad-specific, maternal-effect gene
  JOURNAL   BMC Genomics 11, 721 (2010)
   PUBMED   21172006
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2775)
  AUTHORS   Kaltenbach,L.S., Romero,E., Becklin,R.R., Chettier,R., Bell,R.,
            Phansalkar,A., Strand,A., Torcassi,C., Savage,J., Hurlburt,A.,
            Cha,G.H., Ukani,L., Chepanoske,C.L., Zhen,Y., Sahasrabudhe,S.,
            Olson,J., Kurschner,C., Ellerby,L.M., Peltier,J.M., Botas,J. and
            Hughes,R.E.
  TITLE     Huntingtin interacting proteins are genetic modifiers of
            neurodegeneration
  JOURNAL   PLoS Genet 3 (5), e82 (2007)
   PUBMED   17500595
REFERENCE   10 (bases 1 to 2775)
  AUTHORS   Gray,T.A., Azama,K., Whitmore,K., Min,A., Abe,S. and Nicholls,R.D.
  TITLE     Phylogenetic conservation of the makorin-2 gene, encoding a
            multiple zinc-finger protein, antisense to the RAF1 proto-oncogene
  JOURNAL   Genomics 77 (3), 119-126 (2001)
   PUBMED   11597136
  REMARK    GeneRIF: MKRN2 and RAF1 are co-expressed in tissues and cell lines
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC015715.1 and AK298463.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014160.4.
            
            Summary: This gene encodes a probable E3 ubiquitin ligase
            containing several zinc finger domains, that is a member of the
            makorin RING zinc-finger protein family. This gene overlaps the
            v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) gene in an
            antisense orientation and may have a co-regulatory function with
            RAF1. Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC015715.1, SRR1803612.263536.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000170447.12/ ENSP00000170447.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-699               BC015715.1         16-714
            700-700             AK298463.1         571-571
            701-2775            BC015715.1         716-2790
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.2"
     gene            1..2775
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /note="makorin ring finger protein 2"
                     /db_xref="GeneID:23609"
                     /db_xref="HGNC:HGNC:7113"
                     /db_xref="MIM:608426"
     exon            1..90
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1315
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable E3 ubiquitin-protein ligase makorin-2; RING
                     finger protein 62; makorin RING zinc-finger protein 2;
                     RING-type E3 ubiquitin transferase makorin-2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase makorin-2 isoform 1"
                     /protein_id="NP_054879.3"
                     /db_xref="CCDS:CCDS33702.1"
                     /db_xref="GeneID:23609"
                     /db_xref="HGNC:HGNC:7113"
                     /db_xref="MIM:608426"
                     /translation="MSTKQITCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGY
                     CAYGTRCRYDHTRPSAAAGGAVGTMAHSVPSPAFHSPHPPSEVTASIVKTNSHEPGKR
                     EKRTLVLRDRNLSGMAERKTQPSMVSNPGSCSDPQPSPEMKPHSYLDAIRSGLDDVEA
                     SSSYSNEQQLCPYAAAGECRFGDACVYLHGEVCEICRLQVLHPFDPEQRKAHEKICML
                     TFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCNHTYCLSCIRQWRC
                     AKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGK
                     GTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSQGTVRFFNSVRLWDFIENRESRHVPN
                     NEDVDMTELGDLFMHLSGVESSEP"
     exon            91..219
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /inference="alignment:Splign:2.1.0"
     exon            220..401
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /inference="alignment:Splign:2.1.0"
     exon            402..706
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /inference="alignment:Splign:2.1.0"
     exon            707..921
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1032
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1177
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..2775
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2751..2756
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /note="hexamer: ATTAAA"
     polyA_site      2775
                     /gene="MKRN2"
                     /gene_synonym="HSPC070; RNF62"
                     /note="major polyA site"
ORIGIN      
        1 aaggccgagg cggcagcggc tgcgagaggc ggcggcacga cgacggtccc tcagcccagc
       61 caccatgagc accaagcaga tcacttgcag gtattttatg catggtgtgt gtcgggaagg
      121 aagtcagtgc ctattctcac atgacttggc aaacagcaaa ccgtccacca tctgcaagta
      181 ctaccagaag ggctactgtg cctatggaac tcggtgcaga tatgaccaca cgaggccctc
      241 tgctgcagct ggaggtgctg tgggcaccat ggcccacagt gtgccctccc cagctttcca
      301 cagtcctcac cctccttccg aggtcactgc atccattgtg aaaactaact cacatgaacc
      361 cggaaagcgt gaaaagagaa cattggttct tagagaccga aatctctctg gcatggctga
      421 aaggaagacc cagccgagca tggtgagtaa tccaggcagc tgcagcgacc cccagcccag
      481 ccccgagatg aagccgcatt cctacctgga tgccatcagg agtggccttg atgacgtgga
      541 ggccagcagc tcctacagca acgagcagca gctgtgcccc tacgcagctg ctggggagtg
      601 ccggtttggg gatgcctgtg tctacctgca cggggaggtg tgtgaaatct gtaggctgca
      661 agtcttgcac ccattcgacc cagagcagag gaaggctcat gaaaagatct gcatgttgac
      721 gttcgaacac gagatggaaa aggcctttgc cttccaggca agccaggaca aagtgtgcag
      781 tatctgcatg gaagtgatcc tggagaaggc ctctgcttct gagaggagat ttgggattct
      841 ctccaattgc aatcacacgt actgtttgtc ctgcatccgg cagtggcggt gtgccaaaca
      901 gtttgaaaac ccaatcatta agtcttgtcc agaatgccgt gtgatatcag agtttgtaat
      961 tccaagtgtg tattgggtgg aagatcagaa taaaaagaac gagttgattg aagctttcaa
     1021 acaggggatg gggaaaaaag cctgtaaata ctttgagcaa ggcaagggga cctgcccatt
     1081 tggaagcaaa tgtctttatc gccatgctta ccccgatggg cggctagcag agcctgagaa
     1141 acctcggaaa cagctcagtt ctcaaggcac tgtgaggttc tttaattcag tgcggctctg
     1201 ggatttcatc gagaaccgag aaagccggca tgtccccaac aatgaagatg tcgacatgac
     1261 agagctcggg gacctcttca tgcacctttc tggagtggaa tcatcagaac cctaaagagt
     1321 agatggttgc cctgcatctt gggctccatc ggccgaaact ttcccaagcc agggtgtgcg
     1381 gagcttccct gtactgcagc caaggtgacg tgtgacttgg atttgagtgg agttgggctt
     1441 agccttagtc tcattcaatc tccattatta cagccatggg gaagagtgaa agatataaag
     1501 taacctaatt aaatgtatgg aattgctatt tttatagctg atatagttac acctcaagcc
     1561 cctcaggggt aacaactaac aaacacccaa actgtttgga ttgattgctt taaaaaacaa
     1621 acctggctct tacctttgat cttttcttcc ccagaaatag taaacttgca gctgccccta
     1681 atgcagcata tttttcttac caaaggagtc ttcagcccta taaaaggatt cctctatagt
     1741 gtatttctct agtgtattta gtgtgtcgtc aaaattttga tttatacaga gctttcaaga
     1801 acacacaatg caaagtgagc gcagcatagc tgttaacaaa catacaactt ttttctaggg
     1861 ctttaagggt ggtcattttt ttcaagttct ctcaagtgtc ccaaatcagg gtagcaatct
     1921 tgttgccaca tgtgcagcaa acaaagtgga agtatagatc ttcttctccc ttagggaggc
     1981 tcttgaagga gcaggaggta cagtactggg tagcagtctg gccctcctgt cgtctggttg
     2041 gtgttggggc ctccagccag ggccctctag gggaaccaag cctctgctct cacctgtggg
     2101 ttcttgccca tcagggtaat tgtattgaga actcaaatat acgtgcactt acatgtgtgg
     2161 ttcgtactca agtgatctat tatctagctg caaagcctgg ctttgatttg aaattttgta
     2221 aaaatttcat ggcacccaag gtttctgatt ctgacccagc agtggtcctg aagagagctg
     2281 atggcaagtc ttgtagtcat tttgatttta attgaagggt gagcataacc ttgtgaacca
     2341 gcactagctt gttccaagct ggaatttatc taatctattt ttgtgtttaa aaaagctgta
     2401 cctaccaaat aaataaatag tttataaaat gtattactta aggtattagc tgagtttaga
     2461 gtactttctg cttaattaat ttttatactt aactcttcag tagaggttta caaagagtac
     2521 aaaggttaaa ttacaaattc attcccagcc taggctctgg gcacatttcc tgttcttgaa
     2581 ttctgctcct gaagagggtg aacaaatggg gcattcaagt tgtgagctca gaattacttt
     2641 aaaaggaggt aacagccagc cattacacct aaatttaatt tattttatta aaataacata
     2701 attgagggac catcagataa ctgtattttg tcaggtgcaa taaaaacaaa attaaaaccc
     2761 aaatcatcaa gaaaa
//
