LOCUS       NM_199054               3785 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens MAPK interacting serine/threonine kinase 2 (MKNK2),
            transcript variant 2, mRNA.
ACCESSION   NM_199054
VERSION     NM_199054.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Zhi,L., Chen,C., Zhang,G., Huang,T., He,W., Zhang,J., Chen,D.,
            Liu,J., Zhao,J., Qi,Y., Wang,G., Zhang,W. and Wang,Y.
  TITLE     RBM25 depletion suppresses the growth of colon cancer cells through
            regulating alternative splicing of MNK2
  JOURNAL   Sci China Life Sci 67 (10), 2186-2197 (2024)
   PUBMED   39110401
  REMARK    GeneRIF: RBM25 depletion suppresses the growth of colon cancer
            cells through regulating alternative splicing of MNK2.
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Shiers,S., Sahn,J.J. and Price,T.J.
  TITLE     MNK1 and MNK2 Expression in the Human Dorsal Root and Trigeminal
            Ganglion
  JOURNAL   Neuroscience 515, 96-107 (2023)
   PUBMED   36764601
  REMARK    GeneRIF: MNK1 and MNK2 Expression in the Human Dorsal Root and
            Trigeminal Ganglion.
REFERENCE   3  (bases 1 to 3785)
  AUTHORS   Wang,J., Da,C., Su,Y., Song,R. and Bai,Z.
  TITLE     MKNK2 enhances chemoresistance of ovarian cancer by suppressing
            autophagy via miR-125b
  JOURNAL   Biochem Biophys Res Commun 556, 31-38 (2021)
   PUBMED   33836345
  REMARK    GeneRIF: MKNK2 enhances chemoresistance of ovarian cancer by
            suppressing autophagy via miR-125b.
REFERENCE   4  (bases 1 to 3785)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3785)
  AUTHORS   Liu,H., Gong,Z., Li,K., Zhang,Q., Xu,Z. and Xu,Y.
  TITLE     SRPK1/2 and PP1alpha exert opposite functions by modulating
            SRSF1-guided MKNK2 alternative splicing in colon adenocarcinoma
  JOURNAL   J Exp Clin Cancer Res 40 (1), 75 (2021)
   PUBMED   33602301
  REMARK    GeneRIF: SRPK1/2 and PP1alpha exert opposite functions by
            modulating SRSF1-guided MKNK2 alternative splicing in colon
            adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3785)
  AUTHORS   Knauf,U., Tschopp,C. and Gram,H.
  TITLE     Negative regulation of protein translation by mitogen-activated
            protein kinase-interacting kinases 1 and 2
  JOURNAL   Mol Cell Biol 21 (16), 5500-5511 (2001)
   PUBMED   11463832
REFERENCE   7  (bases 1 to 3785)
  AUTHORS   Scheper,G.C., Morrice,N.A., Kleijn,M. and Proud,C.G.
  TITLE     The mitogen-activated protein kinase signal-integrating kinase Mnk2
            is a eukaryotic initiation factor 4E kinase with high levels of
            basal activity in mammalian cells
  JOURNAL   Mol Cell Biol 21 (3), 743-754 (2001)
   PUBMED   11154262
REFERENCE   8  (bases 1 to 3785)
  AUTHORS   Slentz-Kesler,K., Moore,J.T., Lombard,M., Zhang,J.,
            Hollingsworth,R. and Weiner,M.P.
  TITLE     Identification of the human Mnk2 gene (MKNK2) through protein
            interaction with estrogen receptor beta
  JOURNAL   Genomics 69 (1), 63-71 (2000)
   PUBMED   11013076
REFERENCE   9  (bases 1 to 3785)
  AUTHORS   Waskiewicz,A.J., Flynn,A., Proud,C.G. and Cooper,J.A.
  TITLE     Mitogen-activated protein kinases activate the serine/threonine
            kinases Mnk1 and Mnk2
  JOURNAL   EMBO J 16 (8), 1909-1920 (1997)
   PUBMED   9155017
REFERENCE   10 (bases 1 to 3785)
  AUTHORS   Haribabu,B. and Snyderman,R.
  TITLE     Identification of additional members of human G-protein-coupled
            receptor kinase multigene family
  JOURNAL   Proc Natl Acad Sci U S A 90 (20), 9398-9402 (1993)
   PUBMED   8415712
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA950743.1, BC073140.1,
            AK092536.1 and AL137615.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 13, 2020 this sequence version replaced NM_199054.2.
            
            Summary: This gene encodes a member of the
            calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein
            kinase family, which belongs to the protein kinase superfamily.
            This protein contains conserved DLG (asp-leu-gly) and ENIL
            (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which
            binds importin A and the translation factor scaffold protein
            eukaryotic initiation factor 4G (eIF4G). This protein is one of the
            downstream kinases activated by mitogen-activated protein (MAP)
            kinases. It phosphorylates the eukaryotic initiation factor 4E
            (eIF4E), thus playing important roles in the initiation of mRNA
            translation, oncogenic transformation and malignant cell
            proliferation. In addition to eIF4E, this protein also interacts
            with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1)
            and Cullin2 (Cul2), which are all components of the CBC(VHL)
            ubiquitin ligase E3 complex. Multiple alternatively spliced
            transcript variants have been found, but the full-length nature and
            biological activity of only two variants are determined. These two
            variants encode distinct isoforms which differ in activity and
            regulation, and in subcellular localization. [provided by RefSeq,
            Aug 2011].
            
            Transcript Variant: This variant (2) is the longer transcript and
            it encodes the longer protein (isoform 2, also known as isoform a),
            which is located in the cytoplasm and has an MAP kinase-binding
            region at the C-terminus.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1451105.1,
                                           SRR14038194.3824652.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000250896.9/ ENSP00000250896.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                DA950743.1         1-20
            21-1399             BC073140.1         1-1379
            1400-3408           AK092536.1         303-2311
            3409-3785           AL137615.1         1093-1469
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..3785
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /note="MAPK interacting serine/threonine kinase 2"
                     /db_xref="GeneID:2872"
                     /db_xref="HGNC:HGNC:7111"
                     /db_xref="MIM:605069"
     exon            1..149
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            150..296
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             246..1643
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2; G
                     protein-coupled receptor kinase 7; MAP kinase-interacting
                     serine/threonine-protein kinase 2; MAP kinase
                     signal-integrating kinase 2; MAPK signal-integrating
                     kinase 2; MAP kinase interacting serine/threonine kinase
                     2"
                     /codon_start=1
                     /product="MAP kinase-interacting serine/threonine-protein
                     kinase 2 isoform 2"
                     /protein_id="NP_951009.1"
                     /db_xref="CCDS:CCDS12080.1"
                     /db_xref="GeneID:2872"
                     /db_xref="HGNC:HGNC:7111"
                     /db_xref="MIM:605069"
                     /translation="MVQKKPAELQGFHRSFKGQNPFELAFSLDQPDHGDSDFGLQCSA
                     RPDMPASQPIDIPDAKKRGKKKKRGRATDSFSGRFEDVYQLQEDVLGEGAHARVQTCI
                     NLITSQEYAVKIIEKQPGHIRSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFE
                     KMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQ
                     VSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRC
                     DLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDW
                     AHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRNSCAKDL
                     TSFAAEAIAMNRQLAQHDEDLAEEEAAGQGQPVLVRATSRCLQLSPPSQSKLAQRRQR
                     ASLSSAPVVLVGDHA"
     exon            297..384
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            385..486
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            487..584
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            585..664
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            665..738
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            739..843
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            844..899
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            900..995
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1190
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1355
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1399
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..3785
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3765..3770
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /note="hexamer: AATAAA"
     polyA_site      3785
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /note="major polyA site"
ORIGIN      
        1 cggctcctct cagcggcggt ggcccaggta gaggggtccg cgctggcggc ggcggcggcg
       61 ctgttccccg cgcggtccgc ggagcggggt ccgggctgcg cgacgtgggg cggcggcggc
      121 actgcggccc cggcccaagc ccgaccccgg gtcccctcct cggccgcccc ccgcccggcc
      181 gcccgccctc gggcctcccc ccgggccctc ggtcccctcc cccgctggcg gggcccggac
      241 agaagatggt gcagaagaaa ccagccgaac ttcagggttt ccaccgttcg ttcaaggggc
      301 agaacccctt cgagctggcc ttctccctag accagcccga ccacggagac tctgactttg
      361 gcctgcagtg ctcagcccgc cctgacatgc ccgccagcca gcccattgac atcccggacg
      421 ccaagaagag gggcaagaag aagaagcgcg gccgggccac cgacagcttc tcgggcaggt
      481 ttgaagacgt ctaccagctg caggaagatg tgctggggga gggcgctcat gcccgagtgc
      541 agacctgcat caacctgatc accagccagg agtacgccgt caagatcatt gagaagcagc
      601 caggccacat tcggagcagg gttttcaggg aggtggagat gctgtaccag tgccagggac
      661 acaggaacgt cctagagctg attgagttct tcgaggagga ggaccgcttc tacctggtgt
      721 ttgagaagat gcggggaggc tccatcctga gccacatcca caagcgccgg cacttcaacg
      781 agctggaggc cagcgtggtg gtgcaggacg tggccagcgc cttggacttt ctgcataaca
      841 aaggcatcgc ccacagggac ctaaagccgg aaaacatcct ctgtgagcac cccaaccagg
      901 tctcccccgt gaagatctgt gacttcgacc tgggcagcgg catcaaactc aacggggact
      961 gctcccctat ctccaccccg gagctgctca ctccgtgcgg ctcggcggag tacatggccc
     1021 cggaggtagt ggaggccttc agcgaggagg ctagcatcta cgacaagcgc tgcgacctgt
     1081 ggagcctggg cgtcatcttg tatatcctac tcagcggcta cccgcccttc gtgggccgct
     1141 gtggcagcga ctgcggctgg gaccgcggcg aggcctgccc tgcctgccag aacatgctgt
     1201 ttgagagcat ccaggagggc aagtacgagt tccccgacaa ggactgggcc cacatctcct
     1261 gcgctgccaa agacctcatc tccaagctgc tggtccgtga cgccaagcag aggctgagtg
     1321 ccgcccaagt cctgcagcac ccctgggttc aggggtgcgc cccggagaac accttgccca
     1381 ctcccatggt cctgcagagg aacagctgtg ccaaagacct cacgtccttc gcggctgagg
     1441 ccattgccat gaaccggcag ctggcccagc acgacgagga cctggctgag gaggaggccg
     1501 cggggcaggg ccagcccgtc ctggtccgag ctacctcacg ctgcctgcag ctgtctccac
     1561 cctcccagtc caagctggcg cagcggcggc aaagggccag tctgtcctcg gccccagtgg
     1621 tcctggtggg agaccacgcc tgaccctccc atctcccctc tgtacatagg tcacccgtcc
     1681 cccaatcaaa tctaaaggtt ttttaagcta tcgccagccg gtgtccagcg ggctgcccct
     1741 cctctgcctg gattcccagg cactaagctc agctgagggg ggtgttttat agaaggtttt
     1801 tgcttttggg tttttttttt cctgtttcca cccctccccg ttattttttc ctttggatgg
     1861 ttaaaagcat tgcaggcacc cgggaaggtg agcagagggt aggtgggtgg gcttgtcccc
     1921 tccccggtcc cccgccctgc tcacctctac tatgaaggtg cccccaggtc acctgtgctg
     1981 cccgccatct gcccacgtgg cttgcagtga ctcaggagag caggcccaca gcgtttgcca
     2041 tcttgcagag ctggggaggg gcacaggacc ctgccctcgt gttccctccc agcccgcagt
     2101 atttcaggga caggctcttc ccctctatcc ctcaccctga gagcacccct ggtggcttgg
     2161 ttggggaagg gaggggctgc ctgtctctgg aggtgtcagg caggcaggtg gcaggcagct
     2221 cacccaccca ccccatggga tcccccagcc cttcacccgc gcctgccttg tccccatgat
     2281 agttgacaat cggggcttcc tgcaaggccc gtctgtctgt ccaggactcc tggtggccag
     2341 attcggcctc cgaccttgac cttaaactgc agctgacccc aggggctcgc cgctgcccct
     2401 cccctccaca ccaaggcctg agacagcagg agccccgcct ggcccgaagc cgtttccacc
     2461 gcagcaggca gaggggctgg acaggcactg tcagccaatg tggggggtcc tgaagacacc
     2521 cccttggggc acccgagtgc cccttctcag ggctcagtct gaccgtagcc acgtcctgcc
     2581 tcgcgccgcc cctcgggcct gacctggaag ctccgtcagc tccgtccttg tccttagagc
     2641 tgagcccaga ccccggggtc tggccgaatc ctcaccccca gggcagtgtt tttggtctgc
     2701 caccttcagg aaaacggctg cggcctcggc ctcccttcgg gcacccagga atgcgggggt
     2761 ctgctcagtc cccccaccct ccatgctcca acccccgggg gctgcggagc ctgctgcccc
     2821 ctccccgcgg gtggggacgt tctatgcaat acagggttcc actttagaag tgcgcgcggc
     2881 tagggtcacc gcccgccctt cccggcgcag cccccgagct ccacagctgg ggcagcccct
     2941 ctggcttcta aatccgcggt cgggattctt cctcctgttt agttttttag tttttcctta
     3001 aaaaaaaaca acacatcgat ggactttgct tccctgttct tgaagaatac ttgaatgtcg
     3061 gggggcctgg gggtgggggc ctcggagacc gtctgcctgg ccctgctgcc cctcctgaat
     3121 ctcgtatgat ggtcacagtc cggtggccgt gggggtgctc tgccttccct ggtccccact
     3181 gcccatatct gtggactgcc ccttccaaag acccctgggg ggggtggggc attccgccca
     3241 cccctttccc ccatcacttc tcgcctgtca gtgattccat gtttcgtaac gggggattct
     3301 ctgccttttt gtatcaaaga acaagcaaat ggacccccgc ccgctgcagg cgcccatagc
     3361 catcgggtct ctaaagctga gtggctagca gcgtttgttt gtttgttttt tttttttttt
     3421 ctgaaggtgg gacagtcact tcctcctccc tccccacccc tgtcgcatcc acgtgcgacc
     3481 tggaggactg gtcagaaccg ttactgtgaa tgagtgaaga tcctggagga ccctgggccc
     3541 caggccagct cccatcgctg ggggacggtg aacggccatg tgttaatgtt acgatgtttt
     3601 taaaagacaa aaaaaaaaaa aaaacctcaa aagttttttt aaagtggggg aaaaacatcc
     3661 aagcacttta attccaatgt accaggtgaa ctgacggagc tcagaagttt tcctttacac
     3721 caactgtcaa tgccggaatt ttgtattctg ttttgtaaag atttaataaa agtcaaaaaa
     3781 cttgc
//
