LOCUS       NM_017572               1758 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens MAPK interacting serine/threonine kinase 2 (MKNK2),
            transcript variant 1, mRNA.
ACCESSION   NM_017572
VERSION     NM_017572.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Zhi,L., Chen,C., Zhang,G., Huang,T., He,W., Zhang,J., Chen,D.,
            Liu,J., Zhao,J., Qi,Y., Wang,G., Zhang,W. and Wang,Y.
  TITLE     RBM25 depletion suppresses the growth of colon cancer cells through
            regulating alternative splicing of MNK2
  JOURNAL   Sci China Life Sci 67 (10), 2186-2197 (2024)
   PUBMED   39110401
  REMARK    GeneRIF: RBM25 depletion suppresses the growth of colon cancer
            cells through regulating alternative splicing of MNK2.
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Shiers,S., Sahn,J.J. and Price,T.J.
  TITLE     MNK1 and MNK2 Expression in the Human Dorsal Root and Trigeminal
            Ganglion
  JOURNAL   Neuroscience 515, 96-107 (2023)
   PUBMED   36764601
  REMARK    GeneRIF: MNK1 and MNK2 Expression in the Human Dorsal Root and
            Trigeminal Ganglion.
REFERENCE   3  (bases 1 to 1758)
  AUTHORS   Wang,J., Da,C., Su,Y., Song,R. and Bai,Z.
  TITLE     MKNK2 enhances chemoresistance of ovarian cancer by suppressing
            autophagy via miR-125b
  JOURNAL   Biochem Biophys Res Commun 556, 31-38 (2021)
   PUBMED   33836345
  REMARK    GeneRIF: MKNK2 enhances chemoresistance of ovarian cancer by
            suppressing autophagy via miR-125b.
REFERENCE   4  (bases 1 to 1758)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 1758)
  AUTHORS   Liu,H., Gong,Z., Li,K., Zhang,Q., Xu,Z. and Xu,Y.
  TITLE     SRPK1/2 and PP1alpha exert opposite functions by modulating
            SRSF1-guided MKNK2 alternative splicing in colon adenocarcinoma
  JOURNAL   J Exp Clin Cancer Res 40 (1), 75 (2021)
   PUBMED   33602301
  REMARK    GeneRIF: SRPK1/2 and PP1alpha exert opposite functions by
            modulating SRSF1-guided MKNK2 alternative splicing in colon
            adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1758)
  AUTHORS   Knauf,U., Tschopp,C. and Gram,H.
  TITLE     Negative regulation of protein translation by mitogen-activated
            protein kinase-interacting kinases 1 and 2
  JOURNAL   Mol Cell Biol 21 (16), 5500-5511 (2001)
   PUBMED   11463832
REFERENCE   7  (bases 1 to 1758)
  AUTHORS   Scheper,G.C., Morrice,N.A., Kleijn,M. and Proud,C.G.
  TITLE     The mitogen-activated protein kinase signal-integrating kinase Mnk2
            is a eukaryotic initiation factor 4E kinase with high levels of
            basal activity in mammalian cells
  JOURNAL   Mol Cell Biol 21 (3), 743-754 (2001)
   PUBMED   11154262
REFERENCE   8  (bases 1 to 1758)
  AUTHORS   Slentz-Kesler,K., Moore,J.T., Lombard,M., Zhang,J.,
            Hollingsworth,R. and Weiner,M.P.
  TITLE     Identification of the human Mnk2 gene (MKNK2) through protein
            interaction with estrogen receptor beta
  JOURNAL   Genomics 69 (1), 63-71 (2000)
   PUBMED   11013076
REFERENCE   9  (bases 1 to 1758)
  AUTHORS   Waskiewicz,A.J., Flynn,A., Proud,C.G. and Cooper,J.A.
  TITLE     Mitogen-activated protein kinases activate the serine/threonine
            kinases Mnk1 and Mnk2
  JOURNAL   EMBO J 16 (8), 1909-1920 (1997)
   PUBMED   9155017
REFERENCE   10 (bases 1 to 1758)
  AUTHORS   Haribabu,B. and Snyderman,R.
  TITLE     Identification of additional members of human G-protein-coupled
            receptor kinase multigene family
  JOURNAL   Proc Natl Acad Sci U S A 90 (20), 9398-9402 (1993)
   PUBMED   8415712
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA950743.1, BC073140.1 and
            AL137615.1.
            
            On Jun 2, 2019 this sequence version replaced NM_017572.3.
            
            Summary: This gene encodes a member of the
            calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein
            kinase family, which belongs to the protein kinase superfamily.
            This protein contains conserved DLG (asp-leu-gly) and ENIL
            (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which
            binds importin A and the translation factor scaffold protein
            eukaryotic initiation factor 4G (eIF4G). This protein is one of the
            downstream kinases activated by mitogen-activated protein (MAP)
            kinases. It phosphorylates the eukaryotic initiation factor 4E
            (eIF4E), thus playing important roles in the initiation of mRNA
            translation, oncogenic transformation and malignant cell
            proliferation. In addition to eIF4E, this protein also interacts
            with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1)
            and Cullin2 (Cul2), which are all components of the CBC(VHL)
            ubiquitin ligase E3 complex. Multiple alternatively spliced
            transcript variants have been found, but the full-length nature and
            biological activity of only two variants are determined. These two
            variants encode distinct isoforms which differ in activity and
            regulation, and in subcellular localization. [provided by RefSeq,
            Aug 2011].
            
            Transcript Variant: This variant (1) differs in the 3' exon
            including 3' coding region and 3' UTR, compared to variant 2. The
            resulting protein (isoform 1, also known as isoform b) has a
            shorter and distinct C-terminus, compared to isoform 2. This
            isoform is found in the nucleus and lacks the MAP kinase-binding
            region.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.308504.1,
                                           SRR1163657.502677.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                DA950743.1         1-20
            21-1595             BC073140.1         1-1575
            1596-1758           AL137615.1         1307-1469
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..1758
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /note="MAPK interacting serine/threonine kinase 2"
                     /db_xref="GeneID:2872"
                     /db_xref="HGNC:HGNC:7111"
                     /db_xref="MIM:605069"
     exon            1..149
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            150..296
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             246..1490
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1; G
                     protein-coupled receptor kinase 7; MAP kinase-interacting
                     serine/threonine-protein kinase 2; MAP kinase
                     signal-integrating kinase 2; MAPK signal-integrating
                     kinase 2; MAP kinase interacting serine/threonine kinase
                     2"
                     /codon_start=1
                     /product="MAP kinase-interacting serine/threonine-protein
                     kinase 2 isoform 1"
                     /protein_id="NP_060042.2"
                     /db_xref="CCDS:CCDS12079.1"
                     /db_xref="GeneID:2872"
                     /db_xref="HGNC:HGNC:7111"
                     /db_xref="MIM:605069"
                     /translation="MVQKKPAELQGFHRSFKGQNPFELAFSLDQPDHGDSDFGLQCSA
                     RPDMPASQPIDIPDAKKRGKKKKRGRATDSFSGRFEDVYQLQEDVLGEGAHARVQTCI
                     NLITSQEYAVKIIEKQPGHIRSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFE
                     KMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQ
                     VSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRC
                     DLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDW
                     AHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRWDSHFLL
                     PPHPCRIHVRPGGLVRTVTVNE"
     exon            297..384
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            385..486
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            487..584
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            585..664
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            665..738
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            739..843
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            844..899
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            900..995
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1190
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1355
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1399
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1758
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1738..1743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /note="hexamer: AATAAA"
     polyA_site      1758
                     /gene="MKNK2"
                     /gene_synonym="GPRK7; MNK2"
                     /note="major polyA site"
ORIGIN      
        1 cggctcctct cagcggcggt ggcccaggta gaggggtccg cgctggcggc ggcggcggcg
       61 ctgttccccg cgcggtccgc ggagcggggt ccgggctgcg cgacgtgggg cggcggcggc
      121 actgcggccc cggcccaagc ccgaccccgg gtcccctcct cggccgcccc ccgcccggcc
      181 gcccgccctc gggcctcccc ccgggccctc ggtcccctcc cccgctggcg gggcccggac
      241 agaagatggt gcagaagaaa ccagccgaac ttcagggttt ccaccgttcg ttcaaggggc
      301 agaacccctt cgagctggcc ttctccctag accagcccga ccacggagac tctgactttg
      361 gcctgcagtg ctcagcccgc cctgacatgc ccgccagcca gcccattgac atcccggacg
      421 ccaagaagag gggcaagaag aagaagcgcg gccgggccac cgacagcttc tcgggcaggt
      481 ttgaagacgt ctaccagctg caggaagatg tgctggggga gggcgctcat gcccgagtgc
      541 agacctgcat caacctgatc accagccagg agtacgccgt caagatcatt gagaagcagc
      601 caggccacat tcggagcagg gttttcaggg aggtggagat gctgtaccag tgccagggac
      661 acaggaacgt cctagagctg attgagttct tcgaggagga ggaccgcttc tacctggtgt
      721 ttgagaagat gcggggaggc tccatcctga gccacatcca caagcgccgg cacttcaacg
      781 agctggaggc cagcgtggtg gtgcaggacg tggccagcgc cttggacttt ctgcataaca
      841 aaggcatcgc ccacagggac ctaaagccgg aaaacatcct ctgtgagcac cccaaccagg
      901 tctcccccgt gaagatctgt gacttcgacc tgggcagcgg catcaaactc aacggggact
      961 gctcccctat ctccaccccg gagctgctca ctccgtgcgg ctcggcggag tacatggccc
     1021 cggaggtagt ggaggccttc agcgaggagg ctagcatcta cgacaagcgc tgcgacctgt
     1081 ggagcctggg cgtcatcttg tatatcctac tcagcggcta cccgcccttc gtgggccgct
     1141 gtggcagcga ctgcggctgg gaccgcggcg aggcctgccc tgcctgccag aacatgctgt
     1201 ttgagagcat ccaggagggc aagtacgagt tccccgacaa ggactgggcc cacatctcct
     1261 gcgctgccaa agacctcatc tccaagctgc tggtccgtga cgccaagcag aggctgagtg
     1321 ccgcccaagt cctgcagcac ccctgggttc aggggtgcgc cccggagaac accttgccca
     1381 ctcccatggt cctgcagagg tgggacagtc acttcctcct ccctccccac ccctgtcgca
     1441 tccacgtgcg acctggagga ctggtcagaa ccgttactgt gaatgagtga agatcctgga
     1501 ggaccctggg ccccaggcca gctcccatcg ctgggggacg gtgaacggcc atgtgttaat
     1561 gttacgatgt ttttaaaaga caaaaaaaaa aaaaaaacct caaaagtttt tttaaagtgg
     1621 gggaaaaaca tccaagcact ttaattccaa tgtaccaggt gaactgacgg agctcagaag
     1681 ttttccttta caccaactgt caatgccgga attttgtatt ctgttttgta aagatttaat
     1741 aaaagtcaaa aaacttgc
//
