LOCUS       NM_013255              11136 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens muskelin 1 (MKLN1), transcript variant 2, mRNA.
ACCESSION   NM_013255
VERSION     NM_013255.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Zhu,Y., Meng,X., Zhu,X., Zhang,J., Lv,H., Wang,F., Wang,J.,
            Chen,C., Chen,M., Wang,D., Jin,W., Tian,R. and Wang,R.
  TITLE     Circular RNA MKLN1 promotes epithelial-mesenchymal transition in
            pulmonary fibrosis by regulating the miR-26a/b-5p/CDK8 axis in
            human alveolar epithelial cells and mice models
  JOURNAL   Arch Toxicol 98 (5), 1399-1413 (2024)
   PUBMED   38460002
  REMARK    GeneRIF: Circular RNA MKLN1 promotes epithelial-mesenchymal
            transition in pulmonary fibrosis by regulating the
            miR-26a/b-5p/CDK8 axis in human alveolar epithelial cells and mice
            models.
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   Yang,J., Tan,C., Wang,Y., Zong,T., Xie,T., Yang,Q., Wu,M., Liu,Y.,
            Mu,T., Wang,X. and Yao,Y.
  TITLE     The circRNA MKLN1 regulates autophagy in the development of
            diabetic retinopathy
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1869 (8), 166839 (2023)
   PUBMED   37549719
  REMARK    GeneRIF: The circRNA MKLN1 regulates autophagy in the development
            of diabetic retinopathy.
REFERENCE   3  (bases 1 to 11136)
  AUTHORS   Xu,L., Long,H., Zhou,B., Jiang,H. and Cai,M.
  TITLE     CircMKLN1 Suppresses the Progression of Human Retinoblastoma by
            Modulation of miR-425-5p/PDCD4 Axis
  JOURNAL   Curr Eye Res 46 (11), 1751-1761 (2021)
   PUBMED   33988065
  REMARK    GeneRIF: CircMKLN1 Suppresses the Progression of Human
            Retinoblastoma by Modulation of miR-425-5p/PDCD4 Axis.
REFERENCE   4  (bases 1 to 11136)
  AUTHORS   Sherpa,D., Chrustowicz,J., Qiao,S., Langlois,C.R., Hehl,L.A.,
            Gottemukkala,K.V., Hansen,F.M., Karayel,O., von Gronau,S.,
            Prabu,J.R., Mann,M., Alpi,A.F. and Schulman,B.A.
  TITLE     GID E3 ligase supramolecular chelate assembly configures
            multipronged ubiquitin targeting of an oligomeric metabolic enzyme
  JOURNAL   Mol Cell 81 (11), 2445-2459 (2021)
   PUBMED   33905682
  REMARK    GeneRIF: GID E3 ligase supramolecular chelate assembly configures
            multipronged ubiquitin targeting of an oligomeric metabolic enzyme.
REFERENCE   5  (bases 1 to 11136)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 11136)
  AUTHORS   Prag,S., Collett,G.D. and Adams,J.C.
  TITLE     Molecular analysis of muskelin identifies a conserved
            discoidin-like domain that contributes to protein self-association
  JOURNAL   Biochem J 381 (Pt 2), 547-559 (2004)
   PUBMED   15084145
REFERENCE   7  (bases 1 to 11136)
  AUTHORS   Umeda,M., Nishitani,H. and Nishimoto,T.
  TITLE     A novel nuclear protein, Twa1, and Muskelin comprise a complex with
            RanBPM
  JOURNAL   Gene 303, 47-54 (2003)
   PUBMED   12559565
REFERENCE   8  (bases 1 to 11136)
  AUTHORS   Hasegawa,H., Katoh,H., Fujita,H., Mori,K. and Negishi,M.
  TITLE     Receptor isoform-specific interaction of prostaglandin EP3 receptor
            with muskelin
  JOURNAL   Biochem Biophys Res Commun 276 (1), 350-354 (2000)
   PUBMED   11006128
REFERENCE   9  (bases 1 to 11136)
  AUTHORS   Adams,J.C. and Zhang,L.
  TITLE     cDNA cloning of human muskelin and localisation of the muskelin
            (MKLN1) gene to human chromosome 7q32 and mouse chromosome 6 B1/B2
            by physical mapping and FISH
  JOURNAL   Cytogenet Cell Genet 87 (1-2), 19-21 (1999)
   PUBMED   10640805
REFERENCE   10 (bases 1 to 11136)
  AUTHORS   Adams,J.C., Seed,B. and Lawler,J.
  TITLE     Muskelin, a novel intracellular mediator of cell adhesive and
            cytoskeletal responses to thrombospondin-1
  JOURNAL   EMBO J 17 (17), 4964-4974 (1998)
   PUBMED   9724633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA172589.1, BC067825.1, AK294384.1, BC002834.2, AC009783.9,
            AC008264.10 and AI928463.1.
            
            On Nov 22, 2018 this sequence version replaced NM_013255.4.
            
            Summary: Muskelin is an intracellular protein that acts as a
            mediator of cell spreading and cytoskeletal responses to the
            extracellular matrix component thrombospondin I (MIM 188060) (Adams
            et al., 1998 [PubMed 9724633]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1. The encoded isoform
            (2) has a distinct N-terminus and is longer than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.111870.1,
                                           SRR1803614.158965.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000352689.11/ ENSP00000323527.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 DA172589.1         41-47
            8-1347              BC067825.1         5-1344
            1348-2348           AK294384.1         1461-2461
            2349-2481           BC002834.2         1767-1899
            2482-5043           AC009783.9         141544-144105
            5044-10663          AC008264.10        1-5620
            10664-11136         AI928463.1         19-491              c
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q32.3"
     gene            1..11136
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /note="muskelin 1"
                     /db_xref="GeneID:4289"
                     /db_xref="HGNC:HGNC:7109"
                     /db_xref="MIM:605623"
     exon            1..122
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..2232
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     muskelin 1, intracellular mediator containing kelch
                     motifs"
                     /codon_start=1
                     /product="muskelin isoform 2"
                     /protein_id="NP_037387.2"
                     /db_xref="CCDS:CCDS34754.1"
                     /db_xref="GeneID:4289"
                     /db_xref="HGNC:HGNC:7109"
                     /db_xref="MIM:605623"
                     /translation="MAAGGAVAAAPECRLLPYALHKWSSFSSTYLPENILVDKPNDQS
                     SRWSSESNYPPQYLILKLERPAIVQNITFGKYEKTHVCNLKKFKVFGGMNEENMTELL
                     SSGLKNDYNKETFTLKHKIDEQMFPCRFIKIVPLLSWGPSFNFSIWYVELSGIDDPDI
                     VQPCLNWYSKYREQEAIRLCLKHFRQHNYTEAFESLQKKTKIALEHPMLTDIHDKLVL
                     KGDFDACEELIEKAVNDGLFNQYISQQEYKPRWSQIIPKSTKGDGEDNRPGMRGGHQM
                     VIDVQTETVYLFGGWDGTQDLADFWAYSVKENQWTCISRDTEKENGPSARSCHKMCID
                     IQRRQIYTLGRYLDSSVRNSKSLKSDFYRYDIDTNTWMLLSEDTAADGGPKLVFDHQM
                     CMDSEKHMIYTFGGRILTCNGSVDDSRASEPQFSGLFAFNCQCQTWKLLREDSCNAGP
                     EDIQSRIGHCMLFHSKNRCLYVFGGQRSKTYLNDFFSYDVDSDHVDIISDGTKKDSGM
                     VPMTGFTQRATIDPELNEIHVLSGLSKDKEKREENVRNSFWIYDIVRNSWSCVYKNDQ
                     AAKDNPTKSLQEEEPCPRFAHQLVYDELHKVHYLFGGNPGKSCSPKMRLDDFWSLKLC
                     RPSKDYLLRHCKYLIRKHRFEEKAQVDPLSALKYLQNDLYITVDHSDPEETKEFQLLA
                     SALFKSGSDFTALGFSDVDHTYAQRTQLFDTLVNFFPDSMTPPKGNLVDLITL"
     exon            123..192
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            193..335
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            336..424
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            425..534
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            535..727
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            728..805
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            806..871
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            872..984
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1197
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1419
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..1549
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1550..1697
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1812
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1952
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2055
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2110
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..11136
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11112..11117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /note="hexamer: AATAAA"
     polyA_site      11136
                     /gene="MKLN1"
                     /gene_synonym="TWA2"
                     /note="major polyA site"
ORIGIN      
        1 ggcggccgct acggtgctga caagatggcg gctggcggag ctgtcgctgc ggcgcccgag
       61 tgccggcttc tcccctacgc gctacacaag tggagctcct tttcctccac ctaccttccc
      121 gagaacattt tagtggacaa accaaatgac caatcttcaa gatggtcttc agagagcaac
      181 tatcctcccc agtacttgat tctaaagctc gaaaggcctg ctatagttca gaatatcaca
      241 tttggaaaat atgagaaaac tcatgtttgc aatttgaaga aatttaaagt ctttggtgga
      301 atgaatgaag aaaatatgac agagctgttg tccagtggct taaagaatga ttataacaaa
      361 gaaacattca ccttgaagca taaaattgat gaacagatgt tcccttgtcg attcattaaa
      421 atagttccac tcttgtcctg gggacccagc tttaacttta gcatctggta tgttgaactt
      481 agtggcattg atgatcctga tatagtacaa ccttgtctca actggtatag caagtaccgt
      541 gaacaggaag ctattcgcct ttgcctaaaa cacttcagac aacacaacta tacagaagct
      601 tttgagtcac tgcaaaagaa aaccaagatt gcactggaac atcccatgtt aacagatatt
      661 catgacaagc tggtgttgaa gggtgatttt gatgcttgcg aagagttgat tgaaaaggct
      721 gtaaatgatg gcttgttcaa tcagtatatc agtcaacagg aatataagcc acgatggagt
      781 caaatcattc ccaaaagtac caaaggtgat ggggaagata accgtccagg aatgagagga
      841 ggccatcaga tggttattga tgttcaaaca gagactgttt atttgtttgg tggctgggat
      901 ggaacacaag atcttgctga cttctgggcg tacagtgtga aggagaacca gtggacatgt
      961 atctctagag acactgaaaa agagaatggt cctagtgcca gatcgtgtca taaaatgtgc
     1021 attgatattc aacggaggca aatctacaca ttggggcgtt acttggattc ctctgtgagg
     1081 aacagcaaat ctctgaaaag tgacttctat cgttatgaca ttgatacaaa cacatggatg
     1141 ttactaagtg aggatactgc tgctgatgga gggccgaaat tggtgtttga tcatcagatg
     1201 tgtatggact cagaaaaaca tatgatctac acttttggtg gtagaatttt gacttgtaat
     1261 ggcagcgtag atgacagcag agccagtgaa ccacaattca gtggcttgtt tgctttcaac
     1321 tgtcaatgtc aaacctggaa acttcttcga gaggactcct gtaatgctgg gcctgaggac
     1381 atccagtctc gaataggaca ctgcatgtta ttccactcaa aaaatcgttg cttatatgta
     1441 tttggtggcc agcgatcaaa gacctatttg aatgatttct ttagttatga tgtggactct
     1501 gatcatgtag acataatatc agatggcacc aagaaagact ctgggatggt tccaatgaca
     1561 ggatttacac agagagcaac tattgatcca gaactgaatg aaatacacgt cttatctgga
     1621 ctcagcaaag ataaggaaaa gagggaagaa aatgttagaa attcattctg gatttatgac
     1681 attgtgagga atagttggtc ttgtgtctat aagaatgatc aagctgcaaa ggataatcca
     1741 actaaaagtc ttcaggaaga agaaccatgt ccaaggtttg cccatcagct tgtatacgat
     1801 gagctacaca aggttcatta cttatttggt gggaatccag gaaaatcttg ctctccaaag
     1861 atgagattag atgacttctg gtcactgaag ttgtgtagac cttcaaaaga ttatttactg
     1921 aggcattgca agtacctcat aagaaaacac aggtttgaag aaaaggccca agtggatccc
     1981 cttagtgctc tgaaatattt acaaaatgat ctttatataa ctgtggatca ttcagaccca
     2041 gaagagacaa aagagtttca gctcctggca tcagctctat tcaaatctgg ttcagatttt
     2101 acagctctgg gcttttctga tgtggatcac acctatgctc aaagaactca gctctttgac
     2161 accttagtaa atttctttcc tgacagcatg actcctccta aaggcaacct ggtagacctc
     2221 atcacactgt aactgaagag tcactggaca cagaaatgga aaacaggagt cgattttccg
     2281 tcttttggat tgcagctcca ctgactgaca gtaaagctgc agtgattgag gactgcacca
     2341 gagttctgaa gggatcttaa ccatcacaag tttttaccct cttccttcat gcctgacctc
     2401 aaccccgctc tcctcatcct attcctaaat taggctaata aagtgaaatt ggtatacttt
     2461 ccagttaaat atatatatat atatattttt tcttacttta tcttttaaga attaatgagt
     2521 ataaaagaaa aattaggcag tttacccttt tcagattttt atttcttttt tttttaattg
     2581 ggggaaataa gcactattaa cagattcagc actacaagta ttttgaatgt tgttgctgtt
     2641 ggtcatttgg cctgcctttt cccttcctat ttcactttgc ctcccatcac acaactgcgt
     2701 gtaaatggtg tcacttgttc acctctttac ttcattctta acaagtgtat agttctctag
     2761 gacagttgat gtatgttaag gtgatatctg tgtctgtatt gacttgtctt gtgttacatt
     2821 aaacagtagg cagaactgag gtctcaagtt tgcactcttg gccatagaac taaaaatgaa
     2881 acctgagatt gacccaagca cctatctgag attggtatat aatatttcta taatgatata
     2941 ttttatagta gatattacaa tagcatctga ctttgagtca cagaaacctt aaactgagga
     3001 gacaatagtt cagaaccttt ttaagagcca ttctaaaagt gaccagcagg agggatctag
     3061 tagtgaataa tatcttaata gcaattttag gagaaaatga ctctttcgtc cagaagagct
     3121 ctgcagaacc agggctcccg tttatcttgt gctatctctg tagtattttc atttgagaga
     3181 ggaggctgag tttccagagc tttgctttca tttattttta tacacattgt ctctctatat
     3241 ctgcttgtgg atattgttat tttgaagaga aatggtttgt ggttgtccag tggtatacaa
     3301 ctgacaggca tttcattaag cacatagacc agactttgtg aatggttcag atattttttc
     3361 attaaaaaac aaggtatggc agatggactt ttccatgggt caaatttttt gtgtgagaga
     3421 tagaggtaca tgtcttctga tgatgtgctg tggaatgcaa tgtgggaaac ctacatctgg
     3481 ctagtgctta catttgctca aaaagcatgt tgtatgaagc tgactgccac catgctaaag
     3541 cagattcata ttaagaagtg tcggtcgatc cagtggctct taagagtgaa tagctttatg
     3601 gaatcaagta tgtccctgag tagcaagatt aggcctcacc aaagaagaaa ttaacctgca
     3661 ctttcaaaaa gtaccatgta taggaatgaa acattgaggt cccactttat tctgtcttta
     3721 gtacatggtt ttacttattt tggggatgga tagaaatata tttttgctag ttctaggctt
     3781 gaatcatggg ctccttggct ttcctagcat cccaaagcag tcaacagtga tttcttaagc
     3841 aatttgatct gtacctttac ttgtgaaagg tatgctatat aattcagcaa gtaccaactt
     3901 gtgtagctgc agaataacca agtggctatc cagtcaagta aatacagttt gcttacatac
     3961 ttcaacagtt tcataaaacg attcccctga gtgacacaag aacataaaat gttaatatca
     4021 ctaatatagc ctgttaaatc ttttgtggag acaggtgcaa atcagaagat tgacaaggaa
     4081 gaacttgagc ttgctaaata agacttccta aatttaaaag ctctagtttt gcttagtgtg
     4141 aattctggca ctttaaaaag attaagcaag tgaaattctg ctgccctcca ccatttattt
     4201 ttacagtgct ttgtaatttt tttcatcagt tccttaaatg ttatttggag gaaactaagt
     4261 tcttgacctc agtaatttta tttttgtttt tccctaaatg tttccctact agtctttttg
     4321 gaaaacatgt ttgttttaat ttcatcttcc ctcactttat ttaggtagaa tttttccccc
     4381 ttcatttctg aaattttttg ctcaccgcca tgtttaaatg gggttgaatc atagcgagcc
     4441 atttgctctt gccaaagtga gatagatgta ctgaggagat aattgaaaag tcagactctg
     4501 tactgtgggg ctttaatcgg agcactgctg gaaatgattg cagagaatgt agtgcttcat
     4561 atatccatga ccaagattga catgttgctc acaacatgtc acaatttagt gaggagctac
     4621 atgtatttcc aagaattcca gcatagatta taatttaaat aatcgaattt ggtatttagg
     4681 tacattgttt atatagccaa gtgtatatgc tttactacct aacaattatt ctagcctagg
     4741 tgaatcccta attgaaacac ctggctaaca ctttagacac ataaattaag tgggatacag
     4801 ttttctcatt tgtattatct ccagcttcaa cctcatttat cctttttcct agtataatac
     4861 attgttttca accagaaatt aaagtagata tccaacatta ttttcagtgc ttgtacttca
     4921 tgagaaataa tctgattttc aagacacctc ttaagtgcat ttgcatttta ttttggtaat
     4981 ggcagagacc tcatgtggcc agtttgattg attgaagaat gtatcactct tattcaagca
     5041 aaggaattca gacttaaatg cctacctctg tgctgaagct gacagacagt attaactctt
     5101 atcaaggcca tcttttagac ctgattttca tttatatgaa aacagacctg tccatgtcta
     5161 ataagtaaaa actgatagaa tacattaaaa agtcactggt ttatcataga aaagtttgat
     5221 gggtttagag ctcagtgatt taagcttgta aaactgcatt cttgggcaaa atatttatat
     5281 tgtatataaa tggttggcag cttattccag ttgatctctt cacatctgcc ttaaaagtgc
     5341 tatgtagaga tacattaaca gagttataat aaaacagtgt tttaaaatct agcctataaa
     5401 caaaaactag tttgaggcac ccaagcattt agtgagagat tgagaatgat gcttaatgga
     5461 tcaaaaaagc aattaggagt atgttcctga ttttattctc taaattatac tcaatttcat
     5521 ggtaattaca tgaagaagtt acctgcaatt attcttaaca ctacatggaa ttcactgggt
     5581 aattgtgggt catttatttc ctgaaagtca cgtgaaatcc ttagctggtt gaatgttgca
     5641 cagtatttga gaattacggt ttgaagtgat ttctgtggga gggaatcatt gcaatgtata
     5701 ttctaaataa agtcatctaa ctattaaaaa aaaaacaccc ttcctaaccc ttcttttctt
     5761 aaaattccac attcatccac aatctcatcc ctttgtagaa attcttgcct gaattctcac
     5821 caagttttga attcctaagg tagccccgat ctaggatgtg aaggctgccc agaaaaagtt
     5881 tatggctgga ggagtatcat acagtgtcta catatgatag tacttacaga ttaggtcttt
     5941 ggatgcttta acacaaaaga tttttgttat ccttattagt caaataacgc tattcttttg
     6001 tggttctaga ccctggcttc tatctccctg tgatttgttt taatgctgaa atgacttggc
     6061 tatccaaagc ttctagtcta gaggtctgtt ggttgaaggc agacatttcc aagtttgttg
     6121 aaataatacg aagctgacta gcttacgtga atgatgttgc cctcatttgt tttgggtgag
     6181 gactcattac tgcagtatat tgatctcttc accaaatgct ttttcttttt ctgaataaat
     6241 gctgtattag aggttctatt tatatatgat ttttaaaact ttggtttcct tctatccacc
     6301 aaatactgtg aattgttttt ccatttattt ttcttagcta atgtaacttt attcttcact
     6361 tttttttagc cctagacttc ctagattttc tgtggcattc tgtaggacat tgtattgctt
     6421 ggaaaaaaaa agatcaaaat cattctgggg caaatgctct attatcctta tttatgacaa
     6481 gagaataatg aaattcataa gaaattaata acattcagat attgctataa atgtacttga
     6541 gtcattttca tttggggata gtaataatgg ctgtggcagc tttaatggag aaacctgtgt
     6601 tggcctcttt ttctggcatt aggatctctt gtcatagaac tattggtaaa gtacaggttt
     6661 gatagcagag ttcctgaatt cagcatatca tcagaatttc catttacctt ttgtcttttc
     6721 tttttattgt attttttaac ctttttgttt ctattcctac ctcccatgag taacattgat
     6781 ttctgctgaa gttagaattt gtgttaagaa ttgactttaa acttctgaaa tagttgaata
     6841 ttagaagtgg cttcagttgc catgaaatga ttgcttttct ttttcttttt ttttccttaa
     6901 ataaaaataa tgcagcctta tatatgtctc ttcccctcaa gaatgtgaat ttaggctggg
     6961 catagtgact cacgcctgta atcccagacc tttgggaggc tgacacggga agattgcttg
     7021 agcccaggaa tttcagacca gcctgggcaa cacaggaaga ctccatctct acttaaaata
     7081 tttttgtttt ttagccaggt gtggtggtat gtgcctgtag tcccagctac ttgggaggct
     7141 gaggtgggag gatcacttga acccaggagt ttggggtgca gtgagctatg attgcgacac
     7201 tgcactccag cctgggcaac agagcaagac cctgtctcaa aaaaaaaaaa aaaaaaagaa
     7261 tgtgaattta gctccaattc caaatgacta actcttaggc cagtcatagc caatgaaatc
     7321 atctgacggt agcttctgta gcccttaacc taaggagtca ttagaggtta tagtaaatta
     7381 gtttctctag tggtgcagag agaaatgcca tcggggaaat tatcactgtc tttttggcta
     7441 aagttttata ttgtgaactg gatcccactt aacagcttaa aaacacaaaa atgaaagggt
     7501 agtcctagtt tatcttctta attagaatca ttatttggaa ataagatttg caatgtgctg
     7561 tctatcccca aacactgctg agcttgctgg aaaacagaat ttaacatgaa tgttgtctga
     7621 aagctgtagg atcaatggca aaaaagggga aatgtttggt agggaattgg accagttcta
     7681 aggtatcact tttaattata aaataatgtc ttccttttta tctgcttttc agacagcctc
     7741 agtgaactat taaacatttg tgtgcaaagt cctattttgc ttatctaagg cattttgact
     7801 tggaagtatt aaacttcagg aacgagctag agaagacaat gagggtatgt tagagaagga
     7861 aaaataggag ctttttgctt atggatttta gaggtccaca aatcgaaagg aaaaataaat
     7921 ttcttcattt ctaaccctaa aataattttg ttaatgagat gctgggaaga agactaccaa
     7981 aaggcctcaa agctcatgct tctagaatat tcagtaattc tgaatagtct ttgggaagta
     8041 gcatttcttg ggaagtagca tttcccttct tccaaataac tgtttgagat cttaaaacag
     8101 aaaatccagt gttaatgggc tctagatctt aaactaggtg agcagcttct tacatacaac
     8161 cagttccttg ttaaggatcc agcaggaaaa ccagccataa aacatttcaa catgcagttt
     8221 gggggctccc ttgcttattc actgagttta agctcttgct gcaacatagc tctgtaccat
     8281 ctgggatgct agcagtcaca gcagttagct cagatatctg agctgtctgc cacacaaagc
     8341 ctgaggagat catcattctt cagtttcatt cattacttaa tgaatgccaa attaattggc
     8401 aggaagaaga aacactgtgt gatatactga acaagcctgt gtgcatgaat aaggagccac
     8461 tcaaaaagcc aattttgagc tttatttctc cgttgttaac aaatctcaag aaagcctttg
     8521 ttacagttta gctcatgtca tttggttggt tttattttct agccctttaa cataggagga
     8581 aaattgtcat ttcatgtata acttgtaaca cagaattgaa ctgatactag tttccttgcc
     8641 ttaaattaat tatatgtcat cccaagggtc tctgttaatt ctgctttgcc aagcaataat
     8701 gagatctggg tttggcatta gaagtatttc ataattttgg ttttttattt aggtttcctc
     8761 cacatctgta aagtgattga ttaaattaga ggaggcgtgt agaataaatc ccaatcccat
     8821 tgcaactggc agagctttat aaatctttat aaattcagtt acaacaaagg agaggatcct
     8881 acaccattag agccatgcca tcaggtgttt gcaagtgaca gctgtagtgt gttgcctcaa
     8941 ataataccaa gttataaata ataccaagta attatcaact cactcccaaa tttaataaga
     9001 tatcaaagtc caaaaggtta cttaggagta gtcttccgtg ggggaagata aatttattaa
     9061 agagtcatgt actgatcttt ttcttgggat tttttttcct ttcccagaaa aaaaaattat
     9121 tttggtgact gatcaattgt aaacaatttt cttccttact tacaaatcat ccgttcagaa
     9181 aaataaaagt ggacttcctt tcctaagcat tacaattagc ctgggcaaga ggtgttatga
     9241 ttgtcttatt ctttaagccg gcttactttt tggatttgtg tgaaatgtct tttgaaaaga
     9301 aagtagatag tatagtatta attaactact ttgtatagtc ttctgcataa tttagagttg
     9361 aaaataatgt aacattttcc tgtgaacaga ttcctttatg cgtacatact ttataaatat
     9421 acatacatac acatacacat acatttttaa aatgctcttt ttaaagagat tcttatcttg
     9481 ctcccactcc aaaagctatg tacagttgag ttattagcct cagaggaaga gtacaggaaa
     9541 gcgaagatga ggacctcctt ttcttgtgtt ggtcttggga acaaacagta ttccgtcttt
     9601 caaatatggc atatttctaa acagtttggg aaagatgtta gaatttttta aaagtcagct
     9661 cttttataaa ttggaagtac tcagtagtca gaaattaaga tcccctctga tagagataga
     9721 ttctcaaaac caaaattgga ctgaaaatta gattgagaag aaagatacaa cttcctccat
     9781 agccaataaa atctgtcttt ccaagtctgc ttattaatgc tgtgaatcgc tccttctgtt
     9841 tgtgaaaaca tctcctggac tggaaatgtg gtgctgtaac cagctgagcc gaaagttgga
     9901 gaatgttaaa tatcttatat gggacttacg ataaaatgta ttgatgttag tgattgtcaa
     9961 aataggttta tcatttaaaa aactaggaaa ttactaagta taaagaagaa aaaccagtat
    10021 taattaagtg atgagacctg ttcattttgt tttccctgac ttatttccat taccaaacat
    10081 agtaaaggcc agaaaagatt aaaaaaaaaa ttaacacagc cattccattg ttttttacca
    10141 catggagaaa ggaccaggct ggaagcatat gtccccccac ccttatttat attcctttga
    10201 gcattgccgt tttctagaca gacaatccga aaataattgt gttgataggt ctgtttaagc
    10261 ctgaactctg ggtgtaaaat gttatccaga agctataggg agctgccttg ctcaggtcat
    10321 tggaaatttc aaccctaaga ctcattggaa tttctaagtc cgttatgcac cattatgggc
    10381 atatggagca tattcatacc atgaggagga gtctttgttt atgatggact ttttaatatg
    10441 gccataatta aactcacact caaaaaggat gagctattgt caaaagccaa aagaaaaaca
    10501 gacaaaatga acatgaagca aggagtccat aaaacaagag ttctttacca cgtttaagca
    10561 aatgtctctc aaaaaaaatt gtatttctgt gcacattagc tgagattcac acattttgca
    10621 cctcagtagt atttcagatg agactcaatg tgtggagaaa accgctaact tgtttggttc
    10681 tactcttacc ccctcttctg tggcaatgtg gggtcagggc cgtatcctaa agtatccaca
    10741 tggtcttaaa tggaaatcat gggactctgg aaagctggca tgagctggtt ggagctttta
    10801 aacagaagtg ctttatgggt atcagatctc ttctgacatc aattgtttca aatcattgtt
    10861 gggctctgtc gtgttctgca atcttcccca ttcccctcat gatctctggc ttatctttta
    10921 tccaatctgt atggactttt taggattttt tttttcttat agtacaaata attttcagat
    10981 cccagctttg gtaaatgtgt taattctact ttgtgaataa ataaatgagt gatctgctaa
    11041 aaatggcaat tgtagaatta tgttgtatgt aatgtacata tggagaaaga atgtattttg
    11101 ttcattttct taataaaatg ttatatatgg atgcta
//
