LOCUS       NM_170784               6714 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens MKKS centrosomal shuttling protein (MKKS), transcript
            variant 2, mRNA.
ACCESSION   NM_170784
VERSION     NM_170784.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Beigi,F., Del Pozo-Valero,M., Martin-Merida,I., Perea-Romero,I.,
            Manaviat,M.R., Ayuso,C. and Ghasemi,N.
  TITLE     Apparent but unconfirmed digenism in an Iranian consanguineous
            family with syndromic Retinal Disease
  JOURNAL   Exp Eye Res 207, 108533 (2021)
   PUBMED   33741323
  REMARK    GeneRIF: Apparent but unconfirmed digenism in an Iranian
            consanguineous family with syndromic Retinal Disease.
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 6714)
  AUTHORS   Akimoto,C., Sakashita,E., Kasashima,K., Kuroiwa,K., Tominaga,K.,
            Hamamoto,T. and Endo,H.
  TITLE     Translational repression of the McKusick-Kaufman syndrome
            transcript by unique upstream open reading frames encoding
            mitochondrial proteins with alternative polyadenylation sites
  JOURNAL   Biochim Biophys Acta 1830 (3), 2728-2738 (2013)
   PUBMED   23671934
  REMARK    GeneRIF: Three uORFs (uMKKS0, uMKKS1 and uMKKS2) are reported, and
            they can repress the translation of the downstream MKKS ORF. uMKKS1
            and uMKKS2 are highly conserved in mammals and they encode two
            different mitochondrial membrane proteins respectively.
REFERENCE   4  (bases 1 to 6714)
  AUTHORS   Katsanis,N., Beales,P.L., Woods,M.O., Lewis,R.A., Green,J.S.,
            Parfrey,P.S., Ansley,S.J., Davidson,W.S. and Lupski,J.R.
  TITLE     Mutations in MKKS cause obesity, retinal dystrophy and renal
            malformations associated with Bardet-Biedl syndrome
  JOURNAL   Nat Genet 26 (1), 67-70 (2000)
   PUBMED   10973251
REFERENCE   5  (bases 1 to 6714)
  AUTHORS   Slavotinek,A.M., Stone,E.M., Mykytyn,K., Heckenlively,J.R.,
            Green,J.S., Heon,E., Musarella,M.A., Parfrey,P.S., Sheffield,V.C.
            and Biesecker,L.G.
  TITLE     Mutations in MKKS cause Bardet-Biedl syndrome
  JOURNAL   Nat Genet 26 (1), 15-16 (2000)
   PUBMED   10973238
  REMARK    Erratum:[Nat Genet 2001 Jun;28(2):193]
REFERENCE   6  (bases 1 to 6714)
  AUTHORS   Stone,D.L., Slavotinek,A., Bouffard,G.G., Banerjee-Basu,S.,
            Baxevanis,A.D., Barr,M. and Biesecker,L.G.
  TITLE     Mutation of a gene encoding a putative chaperonin causes
            McKusick-Kaufman syndrome
  JOURNAL   Nat Genet 25 (1), 79-82 (2000)
   PUBMED   10802661
REFERENCE   7  (bases 1 to 6714)
  AUTHORS   Stone,D.L., Agarwala,R., Schaffer,A.A., Weber,J.L., Vaske,D.,
            Oda,T., Chandrasekharappa,S.C., Francomano,C.A. and Biesecker,L.G.
  TITLE     Genetic and physical mapping of the McKusick-Kaufman syndrome
  JOURNAL   Hum Mol Genet 7 (3), 475-481 (1998)
   PUBMED   9467007
REFERENCE   8  (bases 1 to 6714)
  AUTHORS   Gunay-Aygun,M., Gahl,W.A. and Heller,T.
  TITLE     Congenital Hepatic Fibrosis Overview horizontal line RETIRED
            CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301743
REFERENCE   9  (bases 1 to 6714)
  AUTHORS   Slavotinek,A.M.
  TITLE     McKusick-Kaufman Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301675
REFERENCE   10 (bases 1 to 6714)
  AUTHORS   Forsyth,R. and Gunay-Aygun,M.
  TITLE     Bardet-Biedl Syndrome Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301537
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA209180.1, AF221992.1,
            AL034430.4 and AK296439.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_170784.2.
            
            Summary: This gene encodes a protein which shares sequence
            similarity with other members of the type II chaperonin family. The
            encoded protein is a centrosome-shuttling protein and plays an
            important role in cytokinesis. This protein also interacts with
            other type II chaperonin members to form a complex known as the
            BBSome, which involves ciliary membrane biogenesis. This protein is
            encoded by a downstream open reading frame (dORF). Several upstream
            open reading frames (uORFs) have been identified, which repress the
            translation of the dORF, and two of which can encode small
            mitochondrial membrane proteins. Mutations in this gene have been
            observed in patients with Bardet-Biedl syndrome type 6, also known
            as McKusick-Kaufman syndrome. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Feb 2023].
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL157427.1, AK291925.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2150385, SAMEA2151358
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000347364.7/ ENSP00000246062.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            regulatory uORF        :: PMID: 23671934
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                DA209180.1         18-45
            29-1866             AF221992.1         1-1838
            1867-1867           AL034430.4         42107-42107         c
            1868-2533           AF221992.1         1840-2505
            2534-2943           AK296439.1         1143-1552
            2944-6714           AL034430.4         34431-38201         c
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.2"
     gene            1..6714
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /note="MKKS centrosomal shuttling protein"
                     /db_xref="GeneID:8195"
                     /db_xref="HGNC:HGNC:7108"
                     /db_xref="MIM:604896"
     exon            1..115
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /inference="alignment:Splign:2.1.0"
     exon            116..346
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      124..255
                     /regulatory_class="uORF"
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:23671934]"
                     /note="this uORF is known as uMKKS0 and is predicted to
                     encode a 43 aa peptide (PMID:23671934)"
     regulatory      137..328
                     /regulatory_class="uORF"
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:23671934]"
                     /note="this uORF is known as uMKKS1 and encodes a 63 aa
                     protein which is localized to mitochondrial membrane
                     (PMID:23671934)"
     exon            347..1748
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      391..543
                     /regulatory_class="uORF"
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:23671934]"
                     /note="this uORF is known as uMKKS2 and encodes a 50 aa
                     protein which is localized to mitochondrial membrane
                     (PMID:23671934)"
     CDS             764..2476
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /note="McKusick-Kaufman/Bardet-Biedl syndromes putative
                     chaperonin; Bardet-Biedl syndrome 6 protein; alternative
                     protein MKKS"
                     /codon_start=1
                     /product="molecular chaperone MKKS"
                     /protein_id="NP_740754.1"
                     /db_xref="CCDS:CCDS13111.1"
                     /db_xref="GeneID:8195"
                     /db_xref="HGNC:HGNC:7108"
                     /db_xref="MIM:604896"
                     /translation="MSRLEAKKPSLCKSEPLTTERVRTTLSVLKRIVTSCYGPSGRLK
                     QLHNGFGGYVCTTSQSSALLSHLLVTHPILKILTASIQNHVSSFSDCGLFTAILCCNL
                     IENVQRLGLTPTTVIRLNKHLLSLCISYLKSETCGCRIPVDFSSTQILLCLVRSILTS
                     KPACMLTRKETEHVSALILRAFLLTIPENAEGHIILGKSLIVPLKGQRVIDSTVLPGI
                     LIEMSEVQLMRLLPIKKSTALKVALFCTTLSGDTSDTGEGTVVVSYGVSLENAVLDQL
                     LNLGRQLISDHVDLVLCQKVIHPSLKQFLNMHRIIAIDRIGVTLMEPLTKMTGTQPIG
                     SLGSICPNSYGSVKDVCTAKFGSKHFFHLIPNEATICSLLLCNRNDTAWDELKLTCQT
                     ALHVLQLTLKEPWALLGGGCTETHLAAYIRHKTHNDPESILKDDECTQTELQLIAEAF
                     CSALESVVGSLEHDGGEILTDMKYGHLWSVQADSPCVANWPDLLSQCGCGLYNSQEEL
                     NWSFLRSTRRPFVPQSCLPHEAVGSASNLTLDCLTAKLSGLQVAVETANLILDLSYVI
                     EDKN"
     exon            1749..1924
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /inference="alignment:Splign:2.1.0"
     exon            1925..2035
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..6714
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2509..2514
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /note="hexamer: AATAAA"
     polyA_site      2533
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /note="major polyA site"
     regulatory      2779..2784
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /note="hexamer: AATAAA"
     polyA_site      2800
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
     regulatory      6089..6094
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /note="hexamer: AATAAA"
     polyA_site      6115
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
     regulatory      6687..6692
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
                     /note="hexamer: AATAAA"
     polyA_site      6714
                     /gene="MKKS"
                     /gene_synonym="BBS6; HMCS; KMS; MKS"
ORIGIN      
        1 gtgccctcgc gcgacgcgaa ggttgtcggg atccgcggca gcagcggctg cttgagatct
       61 gtttctgggg cctctggcgg tggcggcctg gggcggcgcg acggctggtg cgcaggtaca
      121 ctgatgctga agtactatga gccttcggaa cttgtggaga gactacaaag ttttggttgt
      181 tatggtccct ttagttgggc tcatacattt ggggtggtac agaatcaaaa gcagccctgt
      241 tttccaaata cctaaaaacg acgacattcc tgagcaagat agtctgggac tttcaaatct
      301 tcagaagagc caaatccagg ggaagtagca ggcttgcaat cttcaggtaa agaagcagct
      361 ttgaatctga gcttcatatc gaaagaagag atgaaaaata ccagttggat tagaaagaac
      421 tggcttcttg tagctgggat atctttcata ggtgtccatc ttggaacata ctttttgcag
      481 aggtctgcaa agcagtctgt aaaatttcag tctcaaagca aacaaaagag tattgaagag
      541 tgaagtaaaa taaatatttg gaattactaa tttgtcatta aatcattcta tgctgattag
      601 cttcataaac attgaacttt ttgattttat agccacaatg ctgcatattc atactttaat
      661 tcctaaagaa taatttttaa tgttaaaacg tgataatgca ataaatagaa aaatgtggtt
      721 tacaaaataa aaacggtctt cactagttac cacctgaagt aagatgtctc gtttggaagc
      781 taagaagcca tcattgtgta agagtgaacc actgacaact gagagagtca ggaccacact
      841 ttctgtcttg aaaagaattg taacatcatg ctatggcccc tcaggtaggc tgaagcagct
      901 gcacaatggc tttggaggtt acgtgtgtac aacctcacag tcctcagctc tgctcagtca
      961 ccttttggtc acacatccca ttttaaagat cctgacagcc tccatacaga atcatgtgtc
     1021 aagcttcagt gattgtggct tattcacagc tattctttgc tgcaacctga ttgaaaatgt
     1081 tcagagatta ggcttgacac ccaccactgt cattagatta aataaacatc ttttgagtct
     1141 ttgcatcagt tatctcaagt ctgagacctg tggttgtcga atcccagtgg actttagtag
     1201 tactcagatc ctcctttgtt tggtgcgtag tatattaaca agtaaacctg cctgtatgct
     1261 caccagaaag gaaacagagc atgtcagtgc tttgatcctg agagcctttt tgcttacaat
     1321 tccagaaaat gctgaaggcc acatcatttt aggaaagagt ttaattgtac ctttaaaagg
     1381 tcaaagagtt atagattcca ctgtattacc tgggatactc attgaaatgt cagaagttca
     1441 attaatgagg ctattaccta tcaaaaaatc aactgccctc aaggtggcac tcttttgtac
     1501 aactttatcc ggagacactt ctgacactgg agaaggaact gtggtggtca gttatggggt
     1561 ttctcttgaa aatgcagtct tggaccagct gcttaaccta ggaaggcagc taatcagtga
     1621 ccacgtagat cttgtcctgt gccaaaaagt tatacatcca tctttgaagc agtttctcaa
     1681 tatgcatcgt attattgcca tagacagaat tggagtgact ctgatggaac ccctgactaa
     1741 aatgacagga acacagccta ttggatccct aggctcaata tgtcctaata gttatggaag
     1801 tgtgaaagat gtgtgcactg caaaatttgg ctccaaacat ttttttcatc ttattcctaa
     1861 tgaagcaaca atctgcagct tgcttctctg caacagaaat gacactgcct gggatgagct
     1921 gaagctcacg tgtcagacgg cactgcatgt cctgcagtta acactcaagg aaccatgggc
     1981 tttgttggga ggtggctgta ctgaaactca tttggctgca tatatcagac acaagactca
     2041 caacgaccca gaaagcattc tcaaagatga tgaatgtact caaacagaac ttcaattaat
     2101 tgctgaagca ttttgcagtg ccctagaatc tgttgttggc tctttagaac atgatggagg
     2161 tgaaattctc actgacatga agtatggaca cctttggtca gttcaggcag attctccctg
     2221 tgttgctaac tggccagatt tgctttcaca gtgtggctgt ggattataca atagccagga
     2281 agaactcaac tggtctttct taagaagcac acgtcgtcca tttgtgccac aaagctgcct
     2341 tccacatgaa gctgtgggct cagccagcaa cctgaccttg gactgtttga ctgcaaagct
     2401 tagtggccta caggtggctg tagagacagc caatttgatt ttggatcttt catatgttat
     2461 tgaagataaa aactaagaga atagcatgtt cgtattacaa gagaaacaaa taaactagtc
     2521 tgttggcaat tgagaaaaat tgtgagtgta tttgttttct cccaaagccc tgttctacat
     2581 atttggacaa atgactcata aaattataga tacacttatt taggaaaaaa ggtgattcgt
     2641 gaatggaaat gccatgaaac aataaaaata tgaagcatta tttatttaaa aatattatag
     2701 ttatcttagg gattctatac tggctgctgt acattgttct aaatttttgt tatgttggca
     2761 tcattttgag agcaaacaaa taaaaaagac tcctaatcca tgctctagtt tggatataca
     2821 tattttagat attttccagt tagcagtaat tacatatgct taaagtataa aactagatct
     2881 caaagtgtca caaaacattc agtatatatt gctcaatcaa aagaagtatt caaactgcac
     2941 atttagtaaa atttcacttt tctccatgcc ctttatctca agctatattc ttcagtggtt
     3001 aggtaattga gtagaacctg gtttgcctgc aatttgtagt ttatatcaca actccacatt
     3061 tttgttattt tggggaaatg tttgcttttg gggggaaaaa gtagatttat atagtaacca
     3121 aggctaaagc aacattgtaa tgtcttcatt tttaaagtgg gaagaaggac tggtttgttt
     3181 tacactgggg tttctcaact ttggcattat tgacattctg agccccataa ttctttgttg
     3241 tacggaggct gccctgtgca ttacagctta ctgagcagca tccctggcct ctgcacacta
     3301 gatgccagta gcaccctcct gttgtgacaa ccaaaaatgt ttccagacat tgccaaatgt
     3361 cccccgaaga gcaaaactgt tgttggttga gaaccataga ctgatagaaa aaggaaggac
     3421 aataaggagt attctagcag gacttcagaa agcagcataa agggaagggg aagttatttc
     3481 tctagtcaat agtatgaaaa agaaagtaga aaatctccca tgttcaaagt agagacatta
     3541 tgtttcacca agggatgtag tttgaatttt atgcaaatat aagatgtatt gataaattga
     3601 agaagaaata tagaaggcag ccacatgaat aagcatggag ctataacaaa tgttgtatta
     3661 aactttagga aaatattttg gaaatttttg ggaaacaaac caacctaata gtcaatgatc
     3721 agatctatag ccatgataaa gagaaataga attgttaaac aatttcctct gatattcttg
     3781 taatcaaaaa gaatttttac tctgagccaa aggaaaaaac tagcaataca agcagcaatg
     3841 ccatccatat atgttaggta tcagccccac ctgaagtctc ataaaatatt tatcaaaagt
     3901 tttactcctt ttattattac aatcaaatag aagggcagtg tggtaggcag cctctaaggt
     3961 ggccccacct cctagtttca gaccttgcat aatttcctcc ccttgagcat gggctagact
     4021 tagtgactcc tttatgtctc ccatttccca gctttgtgga cagaatgtta tggtgatggt
     4081 tatggttatg ccttatccat aattttatgc tgtgagtaga aggcagataa cttttctcta
     4141 gtttcatggg tctacagatg gaaagcaact gtgttccagg aactgtactt aaagaactac
     4201 ctcctgagga agcctggggt gattccagtt atgatatcac cctgacttga ttccaattat
     4261 gagattctga actttgagct gatgttgtaa tgcaaaagtg aaacttttga ggatctgagg
     4321 agtagggggg acgtattttg catgtggtag gcagcctcta agatggttcc caataatctc
     4381 acttcctggt ggttcactct ctgggtgtag gtgggaccta atgactagct tctaatgaat
     4441 agaatacagc agaagggaca ggatatcact tctgatattt ggtggtaaaa agatgagttt
     4501 gtctcttgat gccttctctc tcaatggaag ccagctgtca tgttgggaac tgccccctgg
     4561 ggaggcccac atagcaaggg ttgagggagg cttctagcca acagctcaaa aggaactgaa
     4621 tcgtaccaat aaccacaaga gtgaatttgg aagcaggttc tccccagtgg aaccttgagt
     4681 tgagtatagc cctaccactg gagccacacg agagaccctg atgcaaaaaa gccagctaag
     4741 ctactcccag gttggttcct aacccacaaa atctgtgaga taataaatgt tgttttaagt
     4801 caaatttggg atgtactttc ttatacagca atagctatct aataaaggca ggaggaaaag
     4861 cttatttgta ataaattatt tactgaaaac ttggtcacat aaatggaaaa aagcattctt
     4921 gatgccatgc ctttgcattt ttatcatcac cccaacctaa aagaagggtt ctttatcaca
     4981 tgtgtttaca atgtgtcaca taggagttgg gtattgtcct ggttctcttt gcccttatcc
     5041 tgcatttttt caagttcctc caagaagggg tgacacagcg aacctctcca attgatctta
     5101 tagattaggg gcttctactt ttcacgatag ccacttttta aattctatca tttttagctt
     5161 tgccatacaa tttgattaca ttccttagtg aaacatcaat gctcttattt tgaatatatc
     5221 acaaattcgt taaaatatat ttacaaatac ctctatgttt cctaaagtta taataatccc
     5281 tagcagaaaa cactggaaat agaaaaaaag agaaaaaaaa ccttcctcta tagttcaaga
     5341 aaattagcgt tcaaacttag atgataaaac atgaaatgaa aattcttgat gaaaataatt
     5401 ttagaggagt tccttttggc agaaataaat gataagtaaa aatagcaact aaggtaagcc
     5461 tcaggcagtg ttttgtgact taaagtaaga ttgaggtctg aatattgcat tatgaagtac
     5521 ctcaggagag agaatgggaa accgtgatcg ctgtggaaaa ggaaagggag agtgtgaaga
     5581 agaaaatgaa gtcggtgaca gctgctcaat aatttcgtgg gactctcagg acatgtaaca
     5641 ttaaacgtta ttcaaccata ggagtccaac aggattatca tcttattaat caaaaggcat
     5701 taggatttta gcagcaagga agaaagtgga gagctacagc aaataaagga aacttttaat
     5761 caaatactta tttggtctta tagatatcct accctttaat tcttccattt gtaccatatt
     5821 agagagtctt tatatgttag acattgtaag tcaactgtta gactgaagca ctttatgtaa
     5881 gtgaagtatt ttacacaaga tatttaaact atatcacatt aatatctttt attataggta
     5941 tccttgaact catttggttt gtattatcaa agctacttag gcatttacat tgtagacaag
     6001 aaatttgaag taaccataaa tcagccattg tactatgtat tttcatattg ttgttcatat
     6061 tgttgagcag ttctttggat aactttaaaa taaaataatg ttgtttacca tcacatacat
     6121 tatgcaggtt atttaaaatt atcatgtaaa aaatcaatgc aggccttgac aaaacataca
     6181 attagttagg ttattccttc ctgataatct ttggggaata gtcaaagaga ctaaacatct
     6241 cctacaaggc cctgtttcag ctgtctaaat aaagctatct cttaatctga aggaaaagtc
     6301 aattttgaat atctgatatc tttataattt ttttcatgta gctaagtgct tccatttagt
     6361 aactattgta tcccataatg gaagtttttg ccaatcatct acagaatttg aatttagatg
     6421 cacctaaacc caatgttagc ctacttttgg attataagta gatttatatg ctgctttact
     6481 aagaatatag taatattcat gaaagagaat gcaatccctg aaaactattc ctaaatgtaa
     6541 gtgaagctgt ttgttggaga gacgattagt cttggcgcac taaaatccat agaatactat
     6601 attacctgta atgaagtgtg aatttagagg gaaataaaac agcttgacaa tctcctatta
     6661 aaaattctct ttgagaaaat acttataata aacatcttat tttagccatc ttga
//
