LOCUS       NM_002417              12716 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens marker of proliferation Ki-67 (MKI67), transcript
            variant 1, mRNA.
ACCESSION   NM_002417
VERSION     NM_002417.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12716)
  AUTHORS   Luo,H., Lian,Y., Tao,H., Zhao,Y., Wang,Z., Zhou,J., Zhang,Z. and
            Jiang,S.
  TITLE     Relationship between p16/ki67 immunoscores and PAX1/ZNF582
            methylation status in precancerous and cancerous cervical lesions
            in high-risk HPV-positive women
  JOURNAL   BMC Cancer 24 (1), 1171 (2024)
   PUBMED   39304838
  REMARK    GeneRIF: Relationship between p16/ki67 immunoscores and PAX1/ZNF582
            methylation status in precancerous and cancerous cervical lesions
            in high-risk HPV-positive women.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12716)
  AUTHORS   Hernandez-Armendariz,A., Sorichetti,V., Hayashi,Y., Koskova,Z.,
            Brunner,A., Ellenberg,J., Saric,A. and Cuylen-Haering,S.
  TITLE     A liquid-like coat mediates chromosome clustering during mitotic
            exit
  JOURNAL   Mol Cell 84 (17), 3254-3270 (2024)
   PUBMED   39153474
REFERENCE   3  (bases 1 to 12716)
  AUTHORS   Ismael,A.T., Abdulhameed,R.A., Jalal,J.A., Tawfeeq,R.D., Ommar,A.
            and Jalal,A.
  TITLE     Expression of cytokeratin 7/20 and Ki67 in Helicobacter
            pylori-associated gastritis and intestinal metaplasia
  JOURNAL   J Infect Dev Ctries 18 (7), 1032-1040 (2024)
   PUBMED   39078783
  REMARK    GeneRIF: Expression of cytokeratin 7/20 and Ki67 in Helicobacter
            pylori-associated gastritis and intestinal metaplasia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12716)
  AUTHORS   Schmidt,M.H., Broll,R., Bruch,H.P., Finniss,S., Bogler,O. and
            Duchrow,M.
  TITLE     Proliferation marker pKi-67 occurs in different isoforms with
            various cellular effects
  JOURNAL   J Cell Biochem 91 (6), 1280-1292 (2004)
   PUBMED   15048881
REFERENCE   5  (bases 1 to 12716)
  AUTHORS   Duchrow,M., Schluter,C., Wohlenberg,C., Flad,H.D. and Gerdes,J.
  TITLE     Molecular characterization of the gene locus of the human cell
            proliferation-associated nuclear protein defined by monoclonal
            antibody Ki-67
  JOURNAL   Cell Prolif 29 (1), 1-12 (1996)
   PUBMED   8603106
REFERENCE   6  (bases 1 to 12716)
  AUTHORS   Schluter,C., Duchrow,M., Wohlenberg,C., Becker,M.H., Key,G.,
            Flad,H.D. and Gerdes,J.
  TITLE     The cell proliferation-associated antigen of antibody Ki-67: a very
            large, ubiquitous nuclear protein with numerous repeated elements,
            representing a new kind of cell cycle-maintaining proteins
  JOURNAL   J Cell Biol 123 (3), 513-522 (1993)
   PUBMED   8227122
REFERENCE   7  (bases 1 to 12716)
  AUTHORS   Landberg,G. and Roos,G.
  TITLE     Proliferating cell nuclear antigen and Ki-67 antigen expression in
            human haematopoietic cells during growth stimulation and
            differentiation
  JOURNAL   Cell Prolif 26 (5), 427-437 (1993)
   PUBMED   9087533
REFERENCE   8  (bases 1 to 12716)
  AUTHORS   Fonatsch,C., Duchrow,M., Rieder,H., Schluter,C. and Gerdes,J.
  TITLE     Assignment of the human Ki-67 gene (MK167) to 10q25-qter
  JOURNAL   Genomics 11 (2), 476-477 (1991)
   PUBMED   1769665
REFERENCE   9  (bases 1 to 12716)
  AUTHORS   Gerdes,J., Li,L., Schlueter,C., Duchrow,M., Wohlenberg,C.,
            Gerlach,C., Stahmer,I., Kloth,S., Brandt,E. and Flad,H.D.
  TITLE     Immunobiochemical and molecular biologic characterization of the
            cell proliferation-associated nuclear antigen that is defined by
            monoclonal antibody Ki-67
  JOURNAL   Am J Pathol 138 (4), 867-873 (1991)
   PUBMED   2012175
REFERENCE   10 (bases 1 to 12716)
  AUTHORS   Schonk,D.M., Kuijpers,H.J., van Drunen,E., van Dalen,C.H., Geurts
            van Kessel,A.H., Verheijen,R. and Ramaekers,F.C.
  TITLE     Assignment of the gene(s) involved in the expression of the
            proliferation-related Ki-67 antigen to human chromosome 10
  JOURNAL   Hum Genet 83 (3), 297-299 (1989)
   PUBMED   2571566
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL355529.21, X65550.1,
            BU855841.1, AL390236.21 and BU632619.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002417.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X65550.1, SRR14038196.1751110.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368654.8/ ENSP00000357643.3
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-219               AL355529.21        19840-20058         c
            220-2267            X65550.1           2-2049
            2268-2294           BU855841.1         492-518
            2295-3028           X65550.1           2077-2810
            3029-3888           AL355529.21        2001-2860           c
            3889-9675           AL390236.21        117271-123057       c
            9676-10119          AL390236.21        115950-116393       c
            10120-12141         AL390236.21        111926-113947       c
            12142-12716         BU632619.1         15-589              c
FEATURES             Location/Qualifiers
     source          1..12716
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.2"
     gene            1..12716
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="marker of proliferation Ki-67"
                     /db_xref="GeneID:4288"
                     /db_xref="HGNC:HGNC:7107"
                     /db_xref="MIM:176741"
     exon            1..325
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            326..506
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     CDS             415..10185
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proliferation-related Ki-67 antigen; antigen identified by
                     monoclonal antibody Ki-67; protein phosphatase 1,
                     regulatory subunit 105; antigen Ki67; proliferation marker
                     protein Ki-67; Molecular Immunology Borstel antibody 1"
                     /codon_start=1
                     /product="proliferation marker protein Ki-67 isoform 1"
                     /protein_id="NP_002408.3"
                     /db_xref="CCDS:CCDS7659.1"
                     /db_xref="GeneID:4288"
                     /db_xref="HGNC:HGNC:7107"
                     /db_xref="MIM:176741"
                     /translation="MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDIRIQLPVV
                     SKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFRYENESL
                     QNGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNPQVHIK
                     NVKEDSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEISSVKLVSRYGEL
                     KSVPTTQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGLQTDYATEKES
                     ADGLQGETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVESVQTPSKAVG
                     ASFPLYEPAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFKAGDKTLTP
                     RKLSTRNRTPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLT
                     QVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRVSF
                     GGHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQESGSE
                     IHVEVKAQSLVISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRKSGNL
                     PSKRVSISRSQHDILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKHGPQ
                     RSMNKRQRRPATPKKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNF
                     ISNQKMDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPL
                     LPTSESFGGNVFFSAQNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLET
                     KTSDTETEPSKTVSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLL
                     QQRREGEMKEIERPFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPD
                     TELMKDTARGQNLLQTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLG
                     KVGVKEELLAVGKFTRTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKW
                     PRTPKEEAQSLEDLAGFKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQW
                     PKRSLRKADVEEEFLALRKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTL
                     PGSKRQLQTPKEKAQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTP
                     TSTKQRPKRSIRKADVEGELLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKL
                     DLTENLTGSKRRPQTPKEEAQALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPP
                     ESADTPTSTRRQPKTPLEKRDVQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRE
                     TAKQKLDPAASVTGSKRHPKTKEKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIAC
                     RSQPDPVDTPTSSKPQSKRSLRKVDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIY
                     AFMGTPVQKLDLTENLTGSKRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKT
                     AKVACKSSQPDPDKNPASSKRRLKTSLGKVGVKEELLAVGKLTQTSGETTHTHTEPTG
                     DGKSMKAFMESPKQILDSAASLTGSKRQLRTPKGKSEVPEDLAGFIELFQTPSHTKES
                     MTNEKTTKVSYRASQPDLVDTPTSSKPQPKRSLRKADTEEEFLAFRKQTPSAGKAMHT
                     PKPAVGEEKDINTFLGTPVQKLDQPGNLPGSNRRLQTRKEKAQALEELTGFRELFQTP
                     CTDNPTTDEKTTKKILCKSPQSDPADTPTNTKQRPKRSLKKADVEEEFLAFRKLTPSA
                     GKAMHTPKAAVGEEKDINTFVGTPVEKLDLLGNLPGSKRRPQTPKEKAKALEDLAGFK
                     ELFQTPGHTEESMTDDKITEVSCKSPQPDPVKTPTSSKQRLKISLGKVGVKEEVLPVG
                     KLTQTSGKTTQTHRETAGDGKSIKAFKESAKQMLDPANYGTGMERWPRTPKEEAQSLE
                     DLAGFKELFQTPDHTEESTTDDKTTKIACKSPPPESMDTPTSTRRRPKTPLGKRDIVE
                     ELSALKQLTQTTHTDKVPGDEDKGINVFRETAKQKLDPAASVTGSKRQPRTPKGKAQP
                     LEDLAGLKELFQTPICTDKPTTHEKTTKIACRSPQPDPVGTPTIFKPQSKRSLRKADV
                     EEESLALRKRTPSVGKAMDTPKPAGGDEKDMKAFMGTPVQKLDLPGNLPGSKRWPQTP
                     KEKAQALEDLAGFKELFQTPGTDKPTTDEKTTKIACKSPQPDPVDTPASTKQRPKRNL
                     RKADVEEEFLALRKRTPSAGKAMDTPKPAVSDEKNINTFVETPVQKLDLLGNLPGSKR
                     QPQTPKEKAEALEDLVGFKELFQTPGHTEESMTDDKITEVSCKSPQPESFKTSRSSKQ
                     RLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSKSIKAFKESPKQILDPAAS
                     VTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEESMTIDKNTKIPCKSPPPELTDT
                     ATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTHKEPASGDEGIKVLKQRAKKKP
                     NPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHTQESLTAGKATKIPCESPPLE
                     VVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADKEPAGEDKGIKALKESA
                     KQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGHTEESMTDDKTTKIPCKSS
                     PELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETTHTDKEPVGEGKGTKAFK
                     QPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGHTEELANGAADSFTS
                     APKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLPPLPFKRGGGK
                     DGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVVIMKRSLRTSAKRIE
                     PAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQITEVFVLAERIEIN
                     RNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNESSQPKVAEESGGQ
                     KSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVTRSVKRCAENPK
                     KAEDNVCVKKIRTRSHRDSEDI"
     exon            507..585
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            586..701
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            702..768
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            769..814
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            815..1894
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..2070
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2383
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2502
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2674
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            2675..2830
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            2831..9675
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            9676..10119
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     exon            10120..12716
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10212..10217
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="hexamer: AATAAA"
     polyA_site      10233
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="major polyA site"
     regulatory      10747..10752
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="hexamer: AATAAA"
     polyA_site      10767
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="major polyA site"
     regulatory      11470..11475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="hexamer: AATGAA"
     polyA_site      11483
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="major polyA site"
     regulatory      12687..12692
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="hexamer: AATAAA"
     polyA_site      12716
                     /gene="MKI67"
                     /gene_synonym="KIA; MIB-; MIB-1; PPP1R105"
                     /note="major polyA site"
ORIGIN      
        1 agaaggaagt ggagggctga cgctgcgggc gggcgggcgg gcgggaggac tcgactcggt
       61 gggagccgct agagccgggc gcccggggac gtagcctgta gggccaccgg gtccccgtca
      121 gaggcggcgg cgggagcagc ggggactgca ggccggggtg cagcgaacgc gaccccgcgg
      181 gctgcggccc ggtgtgtgcg gagcgtggcg ggcgcagctt accgggcgga ggtgagcgcg
      241 gcgccggctc ctcctgcggc ggactttggg tgcgacttga cgagcggtgg ttcgacaagt
      301 ggccttgcgg gccggatcgt cccagtggaa gagttgtaaa tttgcttctg gccttcccct
      361 acggattata cctggccttc ccctacggat tatactcaac ttactgttta gaaaatgtgg
      421 cccacgagac gcctggttac tatcaaaagg agcggggtcg acggtcccca ctttcccctg
      481 agcctcagca cctgcttgtt tggaaggggt attgaatgtg acatccgtat ccagcttcct
      541 gttgtgtcaa aacaacattg caaaattgaa atccatgagc aggaggcaat attacataat
      601 ttcagttcca caaatccaac acaagtaaat gggtctgtta ttgatgagcc tgtacggcta
      661 aaacatggag atgtaataac tattattgat cgttccttca ggtatgaaaa tgaaagtctt
      721 cagaatggaa ggaagtcaac tgaatttcca agaaaaatac gtgaacagga gccagcacgt
      781 cgtgtctcaa gatctagctt ctcttctgac cctgatgaga aagctcaaga ttccaaggcc
      841 tattcaaaaa tcactgaagg aaaagtttca ggaaatcctc aggtacatat caagaatgtc
      901 aaagaagaca gtaccgcaga tgactcaaaa gacagtgttg ctcagggaac aactaatgtt
      961 cattcctcag aacatgctgg acgtaatggc agaaatgcag ctgatcccat ttctggggat
     1021 tttaaagaaa tttccagcgt taaattagtg agccgttatg gagaattgaa gtctgttccc
     1081 actacacaat gtcttgacaa tagcaaaaaa aatgaatctc ccttttggaa gctttatgag
     1141 tcagtgaaga aagagttgga tgtaaaatca caaaaagaaa atgtcctaca gtattgtaga
     1201 aaatctggat tacaaactga ttacgcaaca gagaaagaaa gtgctgatgg tttacagggg
     1261 gagacccaac tgttggtctc gcgtaagtca agaccaaaat ctggtgggag cggccacgct
     1321 gtggcagagc ctgcttcacc tgaacaagag cttgaccaga acaaggggaa gggaagagac
     1381 gtggagtctg ttcagactcc cagcaaggct gtgggcgcca gctttcctct ctatgagccg
     1441 gctaaaatga agacccctgt acaatattca cagcaacaaa attctccaca aaaacataag
     1501 aacaaagacc tgtatactac tggtagaaga gaatctgtga atctgggtaa aagtgaaggc
     1561 ttcaaggctg gtgataaaac tcttactccc aggaagcttt caactagaaa tcgaacacca
     1621 gctaaagttg aagatgcagc tgactctgcc actaagccag aaaatctctc ttccaaaacc
     1681 agaggaagta ttcctacaga tgtggaagtt ctgcctacgg aaactgaaat tcacaatgag
     1741 ccatttttaa ctctgtggct cactcaagtt gagaggaaga tccaaaagga ttccctcagc
     1801 aagcctgaga aattgggcac tacagctgga cagatgtgct ctgggttacc tggtcttagt
     1861 tcagttgata tcaacaactt tggtgattcc attaatgaga gtgagggaat acctttgaaa
     1921 agaaggcgtg tgtcctttgg tgggcaccta agacctgaac tatttgatga aaacttgcct
     1981 cctaatacgc ctctcaaaag gggagaagcc ccaaccaaaa gaaagtctct ggtaatgcac
     2041 actccacctg tcctgaagaa aatcatcaag gaacagcctc aaccatcagg aaaacaagag
     2101 tcaggttcag aaatccatgt ggaagtgaag gcacaaagct tggttataag ccctccagct
     2161 cctagtccta ggaaaactcc agttgccagt gatcaacgcc gtaggtcctg caaaacagcc
     2221 cctgcttcca gcagcaaatc tcagacagag gttcctaaga gaggagggag aaagagtggc
     2281 aacctgcctt caaagagagt gtctatcagc cgaagtcaac atgatatttt acagatgata
     2341 tgttccaaaa gaagaagtgg tgcttcggaa gcaaatctga ttgttgcaaa atcatgggca
     2401 gatgtagtaa aacttggtgc aaaacaaaca caaactaaag tcataaaaca tggtcctcaa
     2461 aggtcaatga acaaaaggca aagaagacct gctactccaa agaagcctgt gggcgaagtt
     2521 cacagtcaat ttagtacagg ccacgcaaac tctccttgta ccataataat agggaaagct
     2581 catactgaaa aagtacatgt gcctgctcga ccctacagag tgctcaacaa cttcatttcc
     2641 aaccaaaaaa tggactttaa ggaagatctt tcaggaatag ctgaaatgtt caagacccca
     2701 gtgaaggagc aaccgcagtt gacaagcaca tgtcacatcg ctatttcaaa ttcagagaat
     2761 ttgcttggaa aacagtttca aggaactgat tcaggagaag aacctctgct ccccacctca
     2821 gagagttttg gaggaaatgt gttcttcagt gcacagaatg cagcaaaaca gccatctgat
     2881 aaatgctctg caagccctcc cttaagacgg cagtgtatta gagaaaatgg aaacgtagca
     2941 aaaacgccca ggaacaccta caaaatgact tctctggaga caaaaacttc agatactgag
     3001 acagagcctt caaaaacagt atccactgca aacaggtcag gaaggtctac agagttcagg
     3061 aatatacaga agctacctgt ggaaagtaag agtgaagaaa caaatacaga aattgttgag
     3121 tgcatcctaa aaagaggtca gaaggcaaca ctactacaac aaaggagaga aggagagatg
     3181 aaggaaatag aaagaccttt tgagacatat aaggaaaata ttgaattaaa agaaaacgat
     3241 gaaaagatga aagcaatgaa gagatcaaga acttgggggc agaaatgtgc accaatgtct
     3301 gacctgacag acctcaagag cttgcctgat acagaactca tgaaagacac ggcacgtggc
     3361 cagaatctcc tccaaaccca agatcatgcc aaggcaccaa agagtgagaa aggcaaaatc
     3421 actaaaatgc cctgccagtc attacaacca gaaccaataa acaccccaac acacacaaaa
     3481 caacagttga aggcatccct ggggaaagta ggtgtgaaag aagagctcct agcagtcggc
     3541 aagttcacac ggacgtcagg ggagaccacg cacacgcaca gagagccagc aggagatggc
     3601 aagagcatca gaacgtttaa ggagtctcca aagcagatcc tggacccagc agcccgtgta
     3661 actggaatga agaagtggcc aagaacgcct aaggaagagg cccagtcact agaagacctg
     3721 gctggcttca aagagctctt ccagacacca ggtccctctg aggaatcaat gactgatgag
     3781 aaaactacca aaatagcctg caaatctcca ccaccagaat cagtggacac tccaacaagc
     3841 acaaagcaat ggcctaagag aagtctcagg aaagcagatg tagaggaaga attcttagca
     3901 ctcaggaaac taacaccatc agcagggaaa gccatgctta cgcccaaacc agcaggaggt
     3961 gatgagaaag acattaaagc atttatggga actccagtgc agaaactgga cctggcagga
     4021 actttacctg gcagcaaaag acagctacag actcctaagg aaaaggccca ggctctagaa
     4081 gacctggctg gctttaaaga gctcttccag actcctggtc acaccgagga attagtggct
     4141 gctggtaaaa ccactaaaat accctgcgac tctccacagt cagacccagt ggacacccca
     4201 acaagcacaa agcaacgacc caagagaagt atcaggaaag cagatgtaga gggagaactc
     4261 ttagcgtgca ggaatctaat gccatcagca ggcaaagcca tgcacacgcc taaaccatca
     4321 gtaggtgaag agaaagacat catcatattt gtgggaactc cagtgcagaa actggacctg
     4381 acagagaact taaccggcag caagagacgg ccacaaactc ctaaggaaga ggcccaggct
     4441 ctggaagacc tgactggctt taaagagctc ttccagaccc ctggtcatac tgaagaagca
     4501 gtggctgctg gcaaaactac taaaatgccc tgcgaatctt ctccaccaga atcagcagac
     4561 accccaacaa gcacaagaag gcagcccaag acacctttgg agaaaaggga cgtacagaag
     4621 gagctctcag ccctgaagaa gctcacacag acatcagggg aaaccacaca cacagataaa
     4681 gtaccaggag gtgaggataa aagcatcaac gcgtttaggg aaactgcaaa acagaaactg
     4741 gacccagcag caagtgtaac tggtagcaag aggcacccaa aaactaagga aaaggcccaa
     4801 cccctagaag acctggctgg cttgaaagag ctcttccaga caccagtatg cactgacaag
     4861 cccacgactc acgagaaaac taccaaaata gcctgcagat cacaaccaga cccagtggac
     4921 acaccaacaa gctccaagcc acagtccaag agaagtctca ggaaagtgga cgtagaagaa
     4981 gaattcttcg cactcaggaa acgaacacca tcagcaggca aagccatgca cacacccaaa
     5041 ccagcagtaa gtggtgagaa aaacatctac gcatttatgg gaactccagt gcagaaactg
     5101 gacctgacag agaacttaac tggcagcaag agacggctac aaactcctaa ggaaaaggcc
     5161 caggctctag aagacctggc tggctttaaa gagctcttcc agacacgagg tcacactgag
     5221 gaatcaatga ctaacgataa aactgccaaa gtagcctgca aatcttcaca accagaccca
     5281 gacaaaaacc cagcaagctc caagcgacgg ctcaagacat ccctggggaa agtgggcgtg
     5341 aaagaagagc tcctagcagt tggcaagctc acacagacat caggagagac tacacacaca
     5401 cacacagagc caacaggaga tggtaagagc atgaaagcat ttatggagtc tccaaagcag
     5461 atcttagact cagcagcaag tctaactggc agcaagaggc agctgagaac tcctaaggga
     5521 aagtctgaag tccctgaaga cctggccggc ttcatcgagc tcttccagac accaagtcac
     5581 actaaggaat caatgactaa cgaaaaaact accaaagtat cctacagagc ttcacagcca
     5641 gacctagtgg acaccccaac aagctccaag ccacagccca agagaagtct caggaaagca
     5701 gacactgaag aagaattttt agcatttagg aaacaaacgc catcagcagg caaagccatg
     5761 cacacaccca aaccagcagt aggtgaagag aaagacatca acacgttttt gggaactcca
     5821 gtgcagaaac tggaccagcc aggaaattta cctggcagca atagacggct acaaactcgt
     5881 aaggaaaagg cccaggctct agaagaactg actggcttca gagagctttt ccagacacca
     5941 tgcactgata accccacgac tgatgagaaa actaccaaaa aaatactctg caaatctccg
     6001 caatcagacc cagcggacac cccaacaaac acaaagcaac ggcccaagag aagcctcaag
     6061 aaagcagacg tagaggaaga atttttagca ttcaggaaac taacaccatc agcaggcaaa
     6121 gccatgcaca cgcctaaagc agcagtaggt gaagagaaag acatcaacac atttgtgggg
     6181 actccagtgg agaaactgga cctgctagga aatttacctg gcagcaagag acggccacaa
     6241 actcctaaag aaaaggccaa ggctctagaa gatctggctg gcttcaaaga gctcttccag
     6301 acaccaggtc acactgagga atcaatgacc gatgacaaaa tcacagaagt atcctgcaaa
     6361 tctccacaac cagacccagt caaaacccca acaagctcca agcaacgact caagatatcc
     6421 ttggggaaag taggtgtgaa agaagaggtc ctaccagtcg gcaagctcac acagacgtca
     6481 gggaagacca cacagacaca cagagagaca gcaggagatg gaaagagcat caaagcgttt
     6541 aaggaatctg caaagcagat gctggaccca gcaaactatg gaactgggat ggagaggtgg
     6601 ccaagaacac ctaaggaaga ggcccaatca ctagaagacc tggccggctt caaagagctc
     6661 ttccagacac cagaccacac tgaggaatca acaactgatg acaaaactac caaaatagcc
     6721 tgcaaatctc caccaccaga atcaatggac actccaacaa gcacaaggag gcggcccaaa
     6781 acacctttgg ggaaaaggga tatagtggaa gagctctcag ccctgaagca gctcacacag
     6841 accacacaca cagacaaagt accaggagat gaggataaag gcatcaacgt gttcagggaa
     6901 actgcaaaac agaaactgga cccagcagca agtgtaactg gtagcaagag gcagccaaga
     6961 actcctaagg gaaaagccca acccctagaa gacttggctg gcttgaaaga gctcttccag
     7021 acaccaatat gcactgacaa gcccacgact catgagaaaa ctaccaaaat agcctgcaga
     7081 tctccacaac cagacccagt gggtacccca acaatcttca agccacagtc caagagaagt
     7141 ctcaggaaag cagacgtaga ggaagaatcc ttagcactca ggaaacgaac accatcagta
     7201 gggaaagcta tggacacacc caaaccagca ggaggtgatg agaaagacat gaaagcattt
     7261 atgggaactc cagtgcagaa attggacctg ccaggaaatt tacctggcag caaaagatgg
     7321 ccacaaactc ctaaggaaaa ggcccaggct ctagaagacc tggctggctt caaagagctc
     7381 ttccagacac caggcactga caagcccacg actgatgaga aaactaccaa aatagcctgc
     7441 aaatctccac aaccagaccc agtggacacc ccagcaagca caaagcaacg gcccaagaga
     7501 aacctcagga aagcagacgt agaggaagaa tttttagcac tcaggaaacg aacaccatca
     7561 gcaggcaaag ccatggacac accaaaacca gcagtaagtg atgagaaaaa tatcaacaca
     7621 tttgtggaaa ctccagtgca gaaactggac ctgctaggaa atttacctgg cagcaagaga
     7681 cagccacaga ctcctaagga aaaggctgag gctctagagg acctggttgg cttcaaagaa
     7741 ctcttccaga caccaggtca cactgaggaa tcaatgactg atgacaaaat cacagaagta
     7801 tcctgtaaat ctccacagcc agagtcattc aaaacctcaa gaagctccaa gcaaaggctc
     7861 aagatacccc tggtgaaagt ggacatgaaa gaagagcccc tagcagtcag caagctcaca
     7921 cggacatcag gggagactac gcaaacacac acagagccaa caggagatag taagagcatc
     7981 aaagcgttta aggagtctcc aaagcagatc ctggacccag cagcaagtgt aactggtagc
     8041 aggaggcagc tgagaactcg taaggaaaag gcccgtgctc tagaagacct ggttgacttc
     8101 aaagagctct tctcagcacc aggtcacact gaagagtcaa tgactattga caaaaacaca
     8161 aaaattccct gcaaatctcc cccaccagaa ctaacagaca ctgccacgag cacaaagaga
     8221 tgccccaaga cacgtcccag gaaagaagta aaagaggagc tctcagcagt tgagaggctc
     8281 acgcaaacat cagggcaaag cacacacaca cacaaagaac cagcaagcgg tgatgagggc
     8341 atcaaagtat tgaagcaacg tgcaaagaag aaaccaaacc cagtagaaga ggaacccagc
     8401 aggagaaggc caagagcacc taaggaaaag gcccaacccc tggaagacct ggccggcttc
     8461 acagagctct ctgaaacatc aggtcacact caggaatcac tgactgctgg caaagccact
     8521 aaaataccct gcgaatctcc cccactagaa gtggtagaca ccacagcaag cacaaagagg
     8581 catctcagga cacgtgtgca gaaggtacaa gtaaaagaag agccttcagc agtcaagttc
     8641 acacaaacat caggggaaac cacggatgca gacaaagaac cagcaggtga agataaaggc
     8701 atcaaagcat tgaaggaatc tgcaaaacag acaccggctc cagcagcaag tgtaactggc
     8761 agcaggagac ggccaagagc acccagggaa agtgcccaag ccatagaaga cctagctggc
     8821 ttcaaagacc cagcagcagg tcacactgaa gaatcaatga ctgatgacaa aaccactaaa
     8881 ataccctgca aatcatcacc agaactagaa gacaccgcaa caagctcaaa gagacggccc
     8941 aggacacgtg cccagaaagt agaagtgaag gaggagctgt tagcagttgg caagctcaca
     9001 caaacctcag gggagaccac gcacaccgac aaagagccgg taggtgaggg caaaggcacg
     9061 aaagcattta agcaacctgc aaagcggaag ctggacgcag aagatgtaat tggcagcagg
     9121 agacagccaa gagcacctaa ggaaaaggcc caacccctgg aagatctggc cagcttccaa
     9181 gagctctctc aaacaccagg ccacactgag gaactggcaa atggtgctgc tgatagcttt
     9241 acaagcgctc caaagcaaac acctgacagt ggaaaacctc taaaaatatc cagaagagtt
     9301 cttcgggccc ctaaagtaga acccgtggga gacgtggtaa gcaccagaga ccctgtaaaa
     9361 tcacaaagca aaagcaacac ttccctgccc ccactgccct tcaagagggg aggtggcaaa
     9421 gatggaagcg tcacgggaac caagaggctg cgctgcatgc cagcaccaga ggaaattgtg
     9481 gaggagctgc cagccagcaa gaagcagagg gttgctccca gggcaagagg caaatcatcc
     9541 gaacccgtgg tcatcatgaa gagaagtttg aggacttctg caaaaagaat tgaacctgcg
     9601 gaagagctga acagcaacga catgaaaacc aacaaagagg aacacaaatt acaagactcg
     9661 gtccctgaaa ataagggaat atccctgcgc tccagacgcc aaaataagac tgaggcagaa
     9721 cagcaaataa ctgaggtctt tgtattagca gaaagaatag aaataaacag aaatgaaaag
     9781 aagcccatga agacctcccc agagatggac attcagaatc cagatgatgg agcccggaaa
     9841 cccataccta gagacaaagt cactgagaac aaaaggtgct tgaggtctgc tagacagaat
     9901 gagagctccc agcctaaggt ggcagaggag agcggagggc agaagagtgc gaaggttctc
     9961 atgcagaatc agaaagggaa aggagaagca ggaaattcag actccatgtg cctgagatca
    10021 agaaagacaa aaagccagcc tgcagcaagc actttggaga gcaaatctgt gcagagagta
    10081 acgcggagtg tcaagaggtg tgcagaaaat ccaaagaagg ctgaggacaa tgtgtgtgtc
    10141 aagaaaataa gaaccagaag tcatagggac agtgaagata tttgacagaa aaatcgaact
    10201 gggaaaaata taataaagtt agttttgtga taagttctag tgcagttttt gtcataaatt
    10261 acaagtgaat tctgtaagta aggctgtcag tctgcttaag ggaagaaaac tttggatttg
    10321 ctgggtctga atcggcttca taaactccac tgggagcact gctgggctcc tggactgaga
    10381 atagttgaac accgggggct ttgtgaagga gtctgggcca aggtttgccc tcagctttgc
    10441 agaatgaagc cttgaggtct gtcaccaccc acagccaccc tacagcagcc ttaactgtga
    10501 cacttgccac actgtgtcgt cgtttgtttg cctatgtcct ccagggcacg gtggcaggaa
    10561 caactatcct cgtctgtccc aacactgagc aggcactcgg taaacacgaa tgaatggatg
    10621 agcgcacgga tgaatggagc ttacaagatc tgtctttcca atggccgggg gcatttggtc
    10681 cccaaattaa ggctattgga catctgcaca ggacagtcct atttttgatg tcctttcctt
    10741 tctgaaaata aagttttgtg ctttggagaa tgactcgtga gcacatcttt agggaccaag
    10801 agtgactttc tgtaaggagt gactcgtggc ttgccttggt ctcttgggaa tacttttcta
    10861 actagggttg ctctcacctg agacattctc cacccgcgga atctcagggt cccaggctgt
    10921 gggccatcac gacctcaaac tggctcctaa tctccagctt tcctgtcatt gaaagcttcg
    10981 gaagtttact ggctctgctc ccgcctgttt tctttctgac tctatctggc agcccgatgc
    11041 cacccagtac aggaagtgac accagtactc tgtaaagcat catcatcctt ggagagactg
    11101 agcactcagc accttcagcc acgatttcag gatcgcttcc ttgtgagccg ctgcctccga
    11161 aatctccttt gaagcccaga catctttctc cagcttcaga cttgtagata taactcgttc
    11221 atcttcattt actttccact ttgccccctg tcctctctgt gttccccaaa tcagagaata
    11281 gcccgccatc ccccaggtca cctgtctgga ttcctcccca ttcacccacc ttgccaggtg
    11341 caggtgagga tggtgcacca gacagggtag ctgtccccca aaatgtgccc tgtgcgggca
    11401 gtgccctgtc tccacgtttg tttccccagt gtctggcggg gagccaggtg acatcataaa
    11461 tacttgctga atgaatgcag aaatcagcgg tactgacttg tactatattg gctgccatga
    11521 tagggttctc acagcgtcat ccatgatcgt aagggagaat gacattctgc ttgagggagg
    11581 gaatagaaag gggcagggag gggacatctg agggcttcac agggctgcaa agggtacagg
    11641 gattgcacca gggcagaaca ggggagggtg ttcaaggaag agtggctctt agcagaggca
    11701 ctttggaagg tgtgaggcat aaatgcttcc ttctacgtag gccaacctca aaactttcag
    11761 taggaatgtt gctatgatca agttgttcta acactttaga cttagtagta attatgaacc
    11821 tcacatagaa aaatttcatc cagccatatg cctgtggagt ggaatattct gtttagtaga
    11881 aaaatccttt agagttcagc tctaaccaga aatcttgctg aagtatgtca gcaccttttc
    11941 tcaccctggt aagtacagta tttcaagagc acgctaaggg tggttttcat tttacagggc
    12001 tgttgatgat gggttaaaaa tgttcattta agggctaccc ccgtgtttaa tagatgaaca
    12061 ccacttctac acaaccctcc ttggtactgg gggagggaga gatctgacaa atactgccca
    12121 ttcccctagg ctgactggat ttgagaacaa atacccaccc atttccacca tggtatggta
    12181 acttctctga gcttcagttt ccaagtgaat ttccatgtaa taggacattc ccattaaata
    12241 caagctgttt ttactttttc gcctcccagg gcctgtggga tctggtcccc cagcctctct
    12301 tgggctttct tacactaact ctgtacctac catctcctgc ctcccttagg caggcacctc
    12361 caaccaccac acactccctg ctgttttccc tgcctggaac tttccctcct gccccaccaa
    12421 gatcatttca tccagtcctg agctcagctt aagggaggct tcttgcctgt gggttccctc
    12481 acccccatgc ctgtcctcca ggctggggca ggttcttagt ttgcctggaa ttgttctgta
    12541 cctctttgta gcacgtagtg ttgtggaaac taagccacta attgagtttc tggctcccct
    12601 cctggggttg taagttttgt tcattcatga gggccgactg catttcctgg ttactctatc
    12661 ccagtgacca gccacaggag atgtccaata aagtatgtga tgaaatggtc ttaaaa
//
