LOCUS       NM_173481               2885 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens mitotic spindle positioning (MISP), transcript variant
            1, mRNA.
ACCESSION   NM_173481
VERSION     NM_173481.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Vilarinho,T., Padua,D., Pereira,B., Mesquita,P. and Almeida,R.
  TITLE     MISP Is Overexpressed in Intestinal Metaplasia and Gastric Cancer
  JOURNAL   Curr Oncol 31 (5), 2769-2779 (2024)
   PUBMED   38785491
  REMARK    GeneRIF: MISP Is Overexpressed in Intestinal Metaplasia and Gastric
            Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Morales,E.A., Fitz,G.N. and Tyska,M.J.
  TITLE     Mitotic spindle positioning protein (MISP) preferentially binds to
            aged F-actin
  JOURNAL   J Biol Chem 300 (5), 107279 (2024)
   PUBMED   38588808
  REMARK    GeneRIF: Mitotic spindle positioning protein (MISP) preferentially
            binds to aged F-actin.
REFERENCE   3  (bases 1 to 2885)
  AUTHORS   Wang,Q., Liu,W., Leung,C.C., Tarte,D.A. and Gendron,J.M.
  TITLE     Plants distinguish different photoperiods to independently control
            seasonal flowering and growth
  JOURNAL   Science 383 (6683), eadg9196 (2024)
   PUBMED   38330117
  REMARK    GeneRIF: Plants distinguish different photoperiods to independently
            control seasonal flowering and growth.
REFERENCE   4  (bases 1 to 2885)
  AUTHORS   Pan,Y.R., Lai,J.C., Huang,W.K., Peng,P.H., Jung,S.M., Lin,S.H.,
            Chen,C.P., Wu,C.E., Hung,T.H., Yu,A.L., Wu,K.J. and Yeh,C.N.
  TITLE     PLK1 and its substrate MISP facilitate intrahepatic
            cholangiocarcinoma progression by promoting lymphatic invasion and
            impairing E-cadherin adherens junctions
  JOURNAL   Cancer Gene Ther 31 (2), 322-333 (2024)
   PUBMED   38057358
  REMARK    GeneRIF: PLK1 and its substrate MISP facilitate intrahepatic
            cholangiocarcinoma progression by promoting lymphatic invasion and
            impairing E-cadherin adherens junctions.
REFERENCE   5  (bases 1 to 2885)
  AUTHORS   Maarof,N.D., Kumeta,M. and Yoshimura,S.H.
  TITLE     Modulation of actin-binding and -bundling activities of
            MISP/Caprice by multiple phosphorylation
  JOURNAL   Biochem Biophys Res Commun 561, 128-135 (2021)
   PUBMED   34023777
  REMARK    GeneRIF: Modulation of actin-binding and -bundling activities of
            MISP/Caprice by multiple phosphorylation.
REFERENCE   6  (bases 1 to 2885)
  AUTHORS   Kumeta,M., Gilmore,J.L., Umeshima,H., Ishikawa,M., Kitajiri,S.,
            Horigome,T., Kengaku,M. and Takeyasu,K.
  TITLE     Caprice/MISP is a novel F-actin bundling protein critical for
            actin-based cytoskeletal reorganizations
  JOURNAL   Genes Cells 19 (4), 338-349 (2014)
   PUBMED   24475924
  REMARK    GeneRIF: MISP is an effector for cell type-specific actin
            reorganization with its direct actin-binding properties and
            provides a novel model of cell morphology regulation by a
            non-ubiquitous single actin-bundling protein.
            GeneRIF: Bundles F-actin and balances stress fiber-filopodial
            formation
REFERENCE   7  (bases 1 to 2885)
  AUTHORS   Maier,B., Kirsch,M., Anderhub,S., Zentgraf,H. and Kramer,A.
  TITLE     The novel actin/focal adhesion-associated protein MISP is involved
            in mitotic spindle positioning in human cells
  JOURNAL   Cell Cycle 12 (9), 1457-1471 (2013)
   PUBMED   23574715
  REMARK    GeneRIF: MISP links microtubules to the actin cytoskeleton and
            focal adhesions in order to properly position the mitotic spindle.
REFERENCE   8  (bases 1 to 2885)
  AUTHORS   Zhu,M., Settele,F., Kotak,S., Sanchez-Pulido,L., Ehret,L.,
            Ponting,C.P., Gonczy,P. and Hoffmann,I.
  TITLE     MISP is a novel Plk1 substrate required for proper spindle
            orientation and mitotic progression
  JOURNAL   J Cell Biol 200 (6), 773-787 (2013)
   PUBMED   23509069
  REMARK    GeneRIF: Plk1 phosphorylates MISP, thus stabilizing cortical and
            astral microtubule attachments required for proper mitotic spindle
            positioning.
REFERENCE   9  (bases 1 to 2885)
  AUTHORS   Hirano,Y., Ishii,K., Kumeta,M., Furukawa,K., Takeyasu,K. and
            Horigome,T.
  TITLE     Proteomic and targeted analytical identification of BXDC1 and
            EBNA1BP2 as dynamic scaffold proteins in the nucleolus
  JOURNAL   Genes Cells 14 (2), 155-166 (2009)
   PUBMED   19170763
REFERENCE   10 (bases 1 to 2885)
  AUTHORS   Nousiainen,M., Sillje,H.H., Sauer,G., Nigg,E.A. and Korner,R.
  TITLE     Phosphoproteome analysis of the human mitotic spindle
  JOURNAL   Proc Natl Acad Sci U S A 103 (14), 5391-5396 (2006)
   PUBMED   16565220
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004030.2, HY163020.1 and
            BC052236.1.
            
            On Nov 23, 2018 this sequence version replaced NM_173481.3.
            
            Summary: The protein encoded by this gene is an actin-bundling
            protein involved in determining cell morphology and mitotic
            progression. The encoded protein is required for the proper
            positioning of the mitotic spindle. Two transcript variants, one
            protein-coding and the other non-protein coding, have been found
            for this gene. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) represents the longer,
            protein-coding transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052236.1, BX648389.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000215582.8/ ENSP00000215582.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC004030.2         20704-20704
            2-14                HY163020.1         3-15
            15-2885             BC052236.1         1-2871
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..2885
                     /gene="MISP"
                     /gene_synonym="C19orf21; MISP1"
                     /note="mitotic spindle positioning"
                     /db_xref="GeneID:126353"
                     /db_xref="HGNC:HGNC:27000"
                     /db_xref="MIM:615289"
     exon            1..60
                     /gene="MISP"
                     /gene_synonym="C19orf21; MISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            61..1897
                     /gene="MISP"
                     /gene_synonym="C19orf21; MISP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..2157
                     /gene="MISP"
                     /gene_synonym="C19orf21; MISP1"
                     /note="mitotic spindle positioning protein; caprice"
                     /codon_start=1
                     /product="mitotic interactor and substrate of PLK1"
                     /protein_id="NP_775752.1"
                     /db_xref="CCDS:CCDS12042.1"
                     /db_xref="GeneID:126353"
                     /db_xref="HGNC:HGNC:27000"
                     /db_xref="MIM:615289"
                     /translation="MDRVTRYPILGIPQAHRGTGLVLDGDTSYTYHLVCMGPEASGWG
                     QDEPQTWPTDHRAQQGVQRQGVSYSVHAYTGQPSPRGLHSENREDEGWQVYRLGARDA
                     HQGRPTWALRPEDGEDKEMKTYRLDAGDADPRRLCDLERERWAVIQGQAVRKSSTVAT
                     LQGTPDHGDPRTPGPPRSTPLEENVVDREQIDFLAARQQFLSLEQANKGAPHSSPARG
                     TPAGTTPGASQAPKAFNKPHLANGHVVPIKPQVKGVVREENKVRAVPTWASVQVVDDP
                     GSLASVESPGTPKETPIEREIRLAQEREADLREQRGLRQATDHQELVEIPTRPLLTKL
                     SLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRA
                     LSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSL
                     STAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRP
                     LRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERR
                     NALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPV
                     DSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD"
     exon            1898..2028
                     /gene="MISP"
                     /gene_synonym="C19orf21; MISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2067
                     /gene="MISP"
                     /gene_synonym="C19orf21; MISP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2885
                     /gene="MISP"
                     /gene_synonym="C19orf21; MISP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2864..2869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MISP"
                     /gene_synonym="C19orf21; MISP1"
                     /note="hexamer: AATAAA"
     polyA_site      2885
                     /gene="MISP"
                     /gene_synonym="C19orf21; MISP1"
                     /note="major polyA site"
ORIGIN      
        1 ctctgtgccc atcgaggagt cagagtggag gctgcagact gtggagccgg gagccggcag
       61 taagcccaga ggtctccacc ccacgggagg aaggctgagg ccaagacccc ggaagagatg
      121 gaccgcgtga ccagataccc catcctgggc atccctcagg cacaccgtgg caccggcctg
      181 gtgctggatg gagacaccag ctacacatac catctggtgt gcatgggccc cgaggccagc
      241 ggctggggcc aggatgagcc gcagacatgg cccactgacc acagggccca gcagggcgtg
      301 cagaggcagg gggtgtccta cagcgtgcat gcctacactg gccagccgtc cccacggggg
      361 ctccactcgg agaacaggga ggatgagggt tggcaggttt accgcctggg cgccagggat
      421 gcccaccagg gacgtccaac atgggcactc cgcccagagg acggggagga caaggagatg
      481 aagacctacc gcctggatgc tggggacgct gaccccagga ggctgtgtga cctggagcgg
      541 gagcgctggg ccgtcatcca gggccaggca gtcaggaaga gcagcaccgt ggccacgctc
      601 cagggcactc ctgaccacgg agaccccagg acccccggcc cacctcggtc cacgcccctg
      661 gaggagaacg tggttgacag ggagcagatt gacttcctgg cagcgagaca gcagttcctg
      721 agtctggagc aggcgaacaa gggggcccct catagctccc cggccagggg gacccctgca
      781 ggcacaaccc caggggccag ccaggccccc aaggccttca acaagcccca cctggccaac
      841 gggcacgtgg ttcccatcaa gccccaggtg aagggggtgg tcagggaaga gaacaaggtg
      901 cgtgctgtgc ccacctgggc cagtgtccaa gttgtggatg accctggctc cttggcctca
      961 gtggagtccc cggggacccc caaggagacg cccatcgagc gggagatccg tctggctcag
     1021 gagcgtgagg cagacctgcg agagcagagg gggcttcggc aggcaaccga ccaccaggag
     1081 ctggtggaaa tccccaccag gccgctgctg accaagctga gcctgatcac agccccacgg
     1141 cgggagagag ggcgcccgtc cctctacgtg cagcgggaca tagtacagga gacacagcgt
     1201 gaggaagacc accggcggga gggcctgcac gtgggccggg cgtccacacc cgactgggtc
     1261 tcggagggtc cccagcccgg actccggaga gccctcagct cagattccat cctcagcccg
     1321 gccccagatg cccgtgcggc cgacccagct ccagaagtga ggaaggtgaa ccgcatccca
     1381 cctgatgcct accagccgta cctgagcccc gggacccccc agctagaatt ctcagccttc
     1441 ggagcattcg gcaagcccag cagtctctcc acagcggagg ccaaggctgc gacttcacca
     1501 aaggccacga tgtccccgag gcatctctca gaatcctctg gaaaacccct gagcacaaag
     1561 caagaggcat cgaagccccc tcggggatgc ccgcaagcca acaggggtgt cgtgcggtgg
     1621 gagtacttcc gcctgcgtcc tctgcggttc agggccccag acgagcccca gcaggcccaa
     1681 gtcccccatg tctggggctg ggaggtggct ggggcccctg cactgaggct gcagaagtcc
     1741 cagtcatctg atctgctgga aagggagagg gagagtgtcc tgcgccggga gcaagaggtg
     1801 gcagaggagc ggagaaatgc tctcttccca gaggtcttct ccccaacgcc agatgagaac
     1861 tctgaccaga actccaggag ctcctcccag gcatccggca tcacgggcag ttactcggtg
     1921 tctgagtctc ccttcttcag ccccatccac ctacactcaa acgtggcgtg gacagtggaa
     1981 gatccagtgg acagtgctcc tcccgggcag agaaagaagg agcaatggta cgctggcatc
     2041 aacccctcgg acggtatcaa ctcagaggtc ctggaagcca tacgggtgac ccgtcacaag
     2101 aacgccatgg cagagcgctg ggaatcccgc atctacgcca gtgaggagga tgactgagcc
     2161 tcgggatggg gcgcccaccc cctgccctgc cctgaccctc gtgggaactg ccaagaccat
     2221 cgccaagccc ccaccctagg aaatgggtcc taggtccagg atccaagaac cacagctcat
     2281 ctgccaacaa tcccaccatg ggcacatttg ggactgttgg gtttttcgtt tccgtttcta
     2341 tcttccttta gaaatgtttc tgcctttggg gtctaaagct tttggggatg aaatgggacc
     2401 cctgctgatt ctttctgctt ctaagacttt gccaaatgcc ctgggtctaa gaaagaaaga
     2461 gacccgctcc tccactttca ggtgtaattt gcttccgcta gtctgagggc agagggaccg
     2521 gtcaaagagg gtggcacaga tcgcagcacc ttgaggggct gcgggtctga gggaggagac
     2581 actcagctcc tccctctgag aagtcccaag ctgagagggg agacctgccc ctttccaacc
     2641 ctgggaaacc atccagtctg agggaggagg ccaaactccc agtgctgggg gtccctgtgc
     2701 agccctcaaa cccttcacct tggtgcaccc agccacacct ggtggacaca aagctctcac
     2761 atcgatagga tcccatgagg atggtcccct tcacctggga gaaaagtgac ccagtttagg
     2821 agctggaggg gggtctttgt cccccacccc caaactgccc tgaaataaac ctggagtgag
     2881 ctgcc
//
