LOCUS       NM_001258217            2502 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens MIS12 kinetochore complex component (MIS12),
            transcript variant 1, mRNA.
ACCESSION   NM_001258217
VERSION     NM_001258217.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2502)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2502)
  AUTHORS   Wu,Z., Shi,Y., Lu,M., Song,M., Yu,Z., Wang,J., Wang,S., Ren,J.,
            Yang,Y.G., Liu,G.H., Zhang,W., Ci,W. and Qu,J.
  TITLE     METTL3 counteracts premature aging via m6A-dependent stabilization
            of MIS12 mRNA
  JOURNAL   Nucleic Acids Res 48 (19), 11083-11096 (2020)
   PUBMED   33035345
  REMARK    GeneRIF: METTL3 counteracts premature aging via m6A-dependent
            stabilization of MIS12 mRNA.
REFERENCE   5  (bases 1 to 2502)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2502)
  AUTHORS   Kline,S.L., Cheeseman,I.M., Hori,T., Fukagawa,T. and Desai,A.
  TITLE     The human Mis12 complex is required for kinetochore assembly and
            proper chromosome segregation
  JOURNAL   J Cell Biol 173 (1), 9-17 (2006)
   PUBMED   16585270
  REMARK    GeneRIF: These results indicate that a four-subunit Mis12 complex
            plays an essential role in chromosome segregation in vertebrates
            and contributes to mitotic kinetochore assembly.
REFERENCE   7  (bases 1 to 2502)
  AUTHORS   Obuse,C., Iwasaki,O., Kiyomitsu,T., Goshima,G., Toyoda,Y. and
            Yanagida,M.
  TITLE     A conserved Mis12 centromere complex is linked to heterochromatic
            HP1 and outer kinetochore protein Zwint-1
  JOURNAL   Nat Cell Biol 6 (11), 1135-1141 (2004)
   PUBMED   15502821
REFERENCE   8  (bases 1 to 2502)
  AUTHORS   Cheeseman,I.M., Niessen,S., Anderson,S., Hyndman,F., Yates,J.R.
            3rd, Oegema,K. and Desai,A.
  TITLE     A conserved protein network controls assembly of the outer
            kinetochore and its ability to sustain tension
  JOURNAL   Genes Dev 18 (18), 2255-2268 (2004)
   PUBMED   15371340
REFERENCE   9  (bases 1 to 2502)
  AUTHORS   Goshima,G., Kiyomitsu,T., Yoda,K. and Yanagida,M.
  TITLE     Human centromere chromatin protein hMis12, essential for equal
            segregation, is independent of CENP-A loading pathway
  JOURNAL   J Cell Biol 160 (1), 25-39 (2003)
   PUBMED   12515822
  REMARK    GeneRIF: hMIS12 has a role in mitosis and localizes at kinetochores
REFERENCE   10 (bases 1 to 2502)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB258115.1 and AL136906.1.
            
            On Jul 17, 2019 this sequence version replaced NM_001258217.1.
            
            Transcript Variant: Variants 1-5 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853564.11590.1,
                                           SRR11853561.11928.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000611091.5/ ENSP00000484532.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-293               DB258115.1         171-463
            294-2502            AL136906.1         294-2502
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.2"
     gene            1..2502
                     /gene="MIS12"
                     /gene_synonym="2510025F08Rik; hMis12; KNTC2AP; MTW1"
                     /note="MIS12 kinetochore complex component"
                     /db_xref="GeneID:79003"
                     /db_xref="HGNC:HGNC:24967"
                     /db_xref="MIM:609178"
     exon            1..119
                     /gene="MIS12"
                     /gene_synonym="2510025F08Rik; hMis12; KNTC2AP; MTW1"
                     /inference="alignment:Splign:2.1.0"
     exon            120..513
                     /gene="MIS12"
                     /gene_synonym="2510025F08Rik; hMis12; KNTC2AP; MTW1"
                     /inference="alignment:Splign:2.1.0"
     exon            514..2502
                     /gene="MIS12"
                     /gene_synonym="2510025F08Rik; hMis12; KNTC2AP; MTW1"
                     /inference="alignment:Splign:2.1.0"
     CDS             554..1171
                     /gene="MIS12"
                     /gene_synonym="2510025F08Rik; hMis12; KNTC2AP; MTW1"
                     /note="homolog of yeast Mis12; MIS12 homolog; MIS12, MIND
                     kinetochore complex component, homolog"
                     /codon_start=1
                     /product="protein MIS12 homolog"
                     /protein_id="NP_001245146.1"
                     /db_xref="CCDS:CCDS11074.1"
                     /db_xref="GeneID:79003"
                     /db_xref="HGNC:HGNC:24967"
                     /db_xref="MIM:609178"
                     /translation="MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVIL
                     KKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPED
                     KCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFD
                     ELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS"
     regulatory      2486..2491
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIS12"
                     /gene_synonym="2510025F08Rik; hMis12; KNTC2AP; MTW1"
                     /note="hexamer: AATATA"
     polyA_site      2502
                     /gene="MIS12"
                     /gene_synonym="2510025F08Rik; hMis12; KNTC2AP; MTW1"
                     /note="major polyA site"
ORIGIN      
        1 atcagtcgga attttgggga gccaaccgcg ccgtctgtcc ctggcaagcc agcggcggtt
       61 taaaggaggt ggcgggaagc ctgtgtgtgc ttcaaatcgt caccctcatg gtcgctccga
      121 tggcttggct ggaggacaag caaattgagg acattggcaa cggagtgatc aaaatgatag
      181 atcatgaggc ctaaaatgaa taaggaaaga agagaagtgg cagaggctga gaacagaaag
      241 agagggtgga ggggctgtaa atcttgaaga ttagggtata atatgagtat atgggtaaga
      301 attggaagaa ttgtgtagga ggcagtagtc aaaaagtaga agcagtttgg aagagtagtt
      361 acaaatatca agagccaggt ggctaaaagg tggagctata ggtcattgaa gctcaagaaa
      421 ctgagtctct agggcattgg ttaagtcatc tgtctagact tcaaagttgt ctaggatgat
      481 aattcagaag actgatctgt gccaaagtca caggtttttc acgactgaaa acaacatagc
      541 aaaataagcc aagatgtctg tggatccaat gacctacgag gcccagttct ttggcttcac
      601 gccacaaacg tgcatgcttc ggatctacat tgcatttcaa gactacctat ttgaagtgat
      661 gcaggccgtt gaacaggtta ttctgaagaa gctggatggc atcccagact gtgacattag
      721 cccagtgcag attcgcaaat gcacagagaa gtttctttgc ttcatgaaag gacattttga
      781 taaccttttt agcaaaatgg agcaactgtt tttgcagctg attttacgta ttccctcaaa
      841 catcttgctt cctgaagata aatgtaagga gacaccttat agtgaggaag attttcagca
      901 tctccagaaa gaaattgaac agttacagga gaagtacaag actgaattat gtactaagca
      961 ggcccttctt gcagaattag aagagcaaaa aattgttcag gccaaactca aacagacgtt
     1021 gactttcttt gatgagcttc ataatgttgg cagagatcat gggactagtg attttaggga
     1081 gagtttagta tccctggttc agaactccag aaaactacag aacattagag acaatgtgga
     1141 aaaggaatcg aaacgactga aaatatctta attgctcagt agtcaaaagg aggagcctgt
     1201 caaaaagtag aatcataagg actgttcaaa ccataaggac tgttcaaatc ataccagtga
     1261 ctgttcaaac caaccatact ttttattaga tttgctttgt caactctttc ttgtattctg
     1321 tgttttcctc ttttttggtc cactttgctg aggtatgaag tgtactactt tgaactaggc
     1381 tgaagcatct gagtcttcta ataagtggga agggatccaa caaagaagcc atgaccagtt
     1441 aaagatattt gcagagttac accttggtca taagtccttt gtgaccttga ttattttggc
     1501 ttactctttg gatgagacca gacaagaaaa ggattaaacg ggtggctcct ttaatattat
     1561 tattattgtt tttgagacaa ggtccctttc tgtcacccag gttagagtag atttcagtgg
     1621 cacaatcttg gctcactgca acctctgtgt cctgggctca agtgatcctc ctgcctcagc
     1681 ctcccaagta gctaggacca caggtgcgtg tcaccatgct tggctaattt ttttgcagaa
     1741 acgaggcctc actatattgt ccaggctgag tggctctttt attaaccagt cattacactg
     1801 cggaacagcc aacatagagt acttgctctc gtcctgtgaa ttttctttca tgagggagtc
     1861 aatatgtagt ggaaagaagc atgtagcaaa aaagacaacc ttgatcttta ataaaaaaga
     1921 agttggttta tttccaaaat aaatcccctg acaaaaaacc tggtgatgtt aagcaattga
     1981 ctgtcttaga gtccagcaga agaccttaga caaaaaaagc agaacccact ggagtagaaa
     2041 aggaagcatg tagcatatac tcagtagtga aatttaattt tactgactgt taggtatcta
     2101 tgccaatttg ttttcatact tcagttggtt ttggaatctg ccttatacct aatatttatt
     2161 tattcacact cataagcatc aaatatttaa tgccctcagt gggaaatttg tgtttaaact
     2221 caatggaatc taatatttct ttatgtcgtt agtccctgta aaatgttagg tcacccaagg
     2281 aaaggggaga aatagcaatg gttgttccta aggtattgct tgccctccat gtcttcctaa
     2341 agagcagaac ttggagtttc tcctttatgt agagaagaag taacttaggg tgtatttgca
     2401 atgaaatatt catagatatt gaaagcttgt gtttacatga aatatgttta ttatcaagaa
     2461 gtcctttttc caattctgta cattaaatat atgtgtttta aa
//
