LOCUS       NM_001077700            5456 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens MIER1 transcriptional regulator (MIER1), transcript
            variant 2, mRNA.
ACCESSION   NM_001077700
VERSION     NM_001077700.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Wang,S., Fairall,L., Pham,T.K., Ragan,T.J., Vashi,D., Collins,M.O.,
            Dominguez,C. and Schwabe,J.W.R.
  TITLE     A potential histone-chaperone activity for the MIER1 histone
            deacetylase complex
  JOURNAL   Nucleic Acids Res 51 (12), 6006-6019 (2023)
   PUBMED   37099381
  REMARK    GeneRIF: A potential histone-chaperone activity for the MIER1
            histone deacetylase complex.
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 5456)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 5456)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 5456)
  AUTHORS   McCarthy,P.L., Mercer,F.C., Savicky,M.W., Carter,B.A., Paterno,G.D.
            and Gillespie,L.L.
  TITLE     Changes in subcellular localisation of MI-ER1 alpha, a novel
            oestrogen receptor-alpha interacting protein, is associated with
            breast cancer progression
  JOURNAL   Br J Cancer 99 (4), 639-646 (2008)
   PUBMED   18665173
  REMARK    GeneRIF: Loss of nuclear MI-ER1 alpha might contribute to the
            development of invasive breast carcinoma
REFERENCE   6  (bases 1 to 5456)
  AUTHORS   Wang,L., Charroux,B., Kerridge,S. and Tsai,C.C.
  TITLE     Atrophin recruits HDAC1/2 and G9a to modify histone H3K9 and to
            determine cell fates
  JOURNAL   EMBO Rep 9 (6), 555-562 (2008)
   PUBMED   18451879
REFERENCE   7  (bases 1 to 5456)
  AUTHORS   Ding,Z., Gillespie,L.L., Mercer,F.C. and Paterno,G.D.
  TITLE     The SANT domain of human MI-ER1 interacts with Sp1 to interfere
            with GC box recognition and repress transcription from its own
            promoter
  JOURNAL   J Biol Chem 279 (27), 28009-28016 (2004)
   PUBMED   15117948
  REMARK    GeneRIF: the association of hMI-ER1 with Sp1 represents a novel
            mechanism for the negative regulation of Sp1 target promoters
REFERENCE   8  (bases 1 to 5456)
  AUTHORS   Ding,Z., Gillespie,L.L. and Paterno,G.D.
  TITLE     Human MI-ER1 alpha and beta function as transcriptional repressors
            by recruitment of histone deacetylase 1 to their conserved ELM2
            domain
  JOURNAL   Mol Cell Biol 23 (1), 250-258 (2003)
   PUBMED   12482978
  REMARK    GeneRIF: we investigated the role of hMI-ER1alpha and hMI-ER1beta
            in the regulation of transcription.We demonstrate that this
            repressor activity is due to interaction and recruitment of a
            trichostatin A-sensitive histone deacetylase 1 (HDAC1).
REFERENCE   9  (bases 1 to 5456)
  AUTHORS   Paterno,G.D., Ding,Z., Lew,Y.Y., Nash,G.W., Mercer,F.C. and
            Gillespie,L.L.
  TITLE     Genomic organization of the human mi-er1 gene and characterization
            of alternatively spliced isoforms: regulated use of a facultative
            intron determines subcellular localization
  JOURNAL   Gene 295 (1), 79-88 (2002)
   PUBMED   12242014
  REMARK    GeneRIF: Results demonstrate that alternate use of a facultative
            intron regulates the subcellular localization of hMI-ER1 proteins
            and this may have important implications for hMI-ER1 function.
REFERENCE   10 (bases 1 to 5456)
  AUTHORS   Paterno,G.D., Mercer,F.C., Chayter,J.J., Yang,X., Robb,J.D. and
            Gillespie,L.L.
  TITLE     Molecular cloning of human er1 cDNA and its differential expression
            in breast tumours and tumour-derived cell lines
  JOURNAL   Gene 222 (1), 77-82 (1998)
   PUBMED   9813250
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK302061.1, AL500525.4 and
            BM987758.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001077700.2.
            
            Summary: This gene encodes a protein that was first identified in
            Xenopus laevis by its role in a mesoderm induction early response
            (MIER). The encoded protein functions as a transcriptional
            regulator. Alternatively spliced transcript variants encode
            multiple isoforms, some of which lack a C-terminal nuclear
            localization signal. [provided by RefSeq, May 2013].
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            5' coding region and uses an alternate upstream translation start
            site, compared to variant 1. The resulting isoform (b) has a longer
            and distinct N-terminus, compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF515447.1, SRR1660807.66948.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000401041.6/ ENSP00000383820.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1783              AK302061.1         96-1878
            1784-5155           AL500525.4         19021-22392
            5156-5456           BM987758.1         19-319              c
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p31.3"
     gene            1..5456
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /note="MIER1 transcriptional regulator"
                     /db_xref="GeneID:57708"
                     /db_xref="HGNC:HGNC:29657"
                     /db_xref="MIM:616848"
     exon            1..106
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..1737
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /note="isoform b is encoded by transcript variant 2;
                     mesoderm induction early response protein 1; mesoderm
                     induction early response 1 homolog; mesoderm induction
                     early response 1, transcriptional regulator"
                     /codon_start=1
                     /product="mesoderm induction early response protein 1
                     isoform b"
                     /protein_id="NP_001071168.2"
                     /db_xref="CCDS:CCDS53326.1"
                     /db_xref="GeneID:57708"
                     /db_xref="HGNC:HGNC:29657"
                     /db_xref="MIM:616848"
                     /translation="MDGASSGGGGSSEGGGGSSGSGYGVVARFSQCLAEFRTWLRTNW
                     LRFNADKTDVMLPSVESSSPGGSATSDDHEFDPSADMLVHDFDDERTLEEEEMMEGET
                     NFSSEIEDLAREGDMPIHELLSLYGYGSTVRLPEEDEEEEEEEEEGEDDEDADNDDNS
                     GCSGENKEENIKDSSGQEDETQSSNDDPSQSVASQDAQEIIRPRRCKYFDTNSEVEEE
                     SEEDEDYIPSEDWKKEIMVGSMFQAEIPVGICRYKENEKVYENDDQLLWDPEYLPEDK
                     VIIFLKDASRRTGDEKGVEAIPEGSHIKDNEQALYELVKCNFDTEEALRRLRFNVKAA
                     REELSVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKSERYDFF
                     AQQTRFGKKKYNLHPGVTDYMDRLLDESESAASSRAPSPPPTASNSSNSQSEKEDGTV
                     STANQNGVSSNGPGEILNKEEVKVEGLHINGPTGGNKKPLHADMDTNGYETDNLTTDP
                     KLAHMTARNENDFDEKSERPAKRRRVNSNGKESPGSSEFFQEAVSHGKFEELENTDD"
     exon            107..207
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            208..232
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            233..378
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            379..540
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            541..673
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            674..738
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            739..811
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            812..963
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1045
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1140
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1268
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1408
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..5456
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4481..4486
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /note="hexamer: ATTAAA"
     polyA_site      4503
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /note="major polyA site"
     regulatory      4845..4850
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /note="hexamer: AATAAA"
     polyA_site      4868
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
     regulatory      5435..5440
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
                     /note="hexamer: ATTAAA"
     polyA_site      5456
                     /gene="MIER1"
                     /gene_synonym="ER1; MI-ER1"
ORIGIN      
        1 aggcccctcc caggctctga gtctcccggc tgcaggcgga tggatggggc ttcttcaggc
       61 ggtggcggca gcagcgaagg tggcggcggc agcagcggca gcggctatgg tgtggtcgct
      121 cgattctccc agtgcctggc tgagtttcgg acgtggttaa gaaccaactg gttgaggttc
      181 aatgcagaca agacggatgt gatgctgcca tctgttgaat cttcaagtcc aggaggttca
      241 gcaacatcag atgaccatga atttgatcca tcagctgaca tgctggttca tgattttgat
      301 gatgaacgaa cattagaaga ggaagaaatg atggaaggag aaacaaactt cagctctgaa
      361 atagaagatc ttgcaaggga aggcgacatg ccaattcatg aacttctcag cctttatggt
      421 tatggtagta ctgttcgact acctgaagaa gatgaggaag aggaagaaga ggaagaagaa
      481 ggtgaagatg atgaagatgc tgataatgat gacaacagtg gctgtagtgg ggaaaataaa
      541 gaggagaata taaaggattc atcaggtcag gaggatgaaa ctcagtcttc caatgatgat
      601 ccatcacaat ctgttgcttc tcaagatgcc caggaaataa tccgcccacg tcgatgtaaa
      661 tattttgata caaatagtga agtagaagaa gaatctgaag aagatgaaga ttatattcca
      721 tcagaagact ggaaaaagga gattatggtg ggctccatgt ttcaagcaga aattccagtt
      781 ggcatttgta gatacaaaga aaatgaaaaa gtatatgaaa atgatgatca gctcctgtgg
      841 gaccctgagt acttaccaga agataaagtg attatatttc ttaaagatgc atctagaaga
      901 acaggtgatg agaagggtgt agaagcaatt cctgaaggat ctcacataaa agacaatgaa
      961 caggctttat atgaattggt taaatgcaat tttgatacag aagaagcatt gagaagatta
     1021 agatttaatg taaaagcagc tagagaggaa ttatctgttt ggacagagga agagtgtaga
     1081 aattttgaac aagggctgaa ggcctatgga aaggattttc atttgattca ggctaataaa
     1141 gtccgaacaa ggtcagttgg tgaatgtgta gcattctatt acatgtggaa aaaatctgaa
     1201 cgttatgatt tctttgctca gcaaacacga tttggaaaga agaaatataa tcttcatcct
     1261 ggtgtaacgg attacatgga tcgtcttcta gacgaaagtg aaagtgctgc atctagtcga
     1321 gcaccatccc ctcccccaac tgcatcaaac agtagtaaca gccagtctga gaaagaagat
     1381 ggcactgtaa gcactgctaa tcaaaatgga gtgtcatcta atggaccagg tgaaatatta
     1441 aacaaagagg aagtaaaagt tgaagggtta cacattaatg gaccaacagg tggaaataag
     1501 aaaccacttc atgcagatat ggatactaat ggttatgaaa cagataacct taccactgac
     1561 ccaaaacttg cccatatgac tgcaagaaat gaaaatgatt ttgatgaaaa aagtgagaga
     1621 cctgccaaaa ggcgaagggt aaacagcaat ggaaaagaaa gtccaggttc ttctgaattt
     1681 ttccaagaag cagtctcaca tgggaaattt gaagaacttg aaaacacaga tgactaaatt
     1741 ttagacctat tttactttct ttggagtaaa ttctggtgtg actaaaattt tcagggttga
     1801 tgggttacct taaaaagttg atttccttga agcagtttga aaatttgaat tgagtcttaa
     1861 ctttaggaaa tgaggtttca taattctgcc ttttgcagat ttttttactt taaagctgtc
     1921 agactctttt aagggtattt taaaaattag taaatttgaa tgaactaaag atatatctct
     1981 accttctcat ttgaatatct ttagttcatt cagatttact aattttggta aatctgaatg
     2041 aactaaagat gtatctgtac cttctcattt gatgtattaa tcataaaatt tcactacaag
     2101 gtaatttttg cttagtttga tggatgaatg ggaaactcaa gtccaaattt ctgcttaata
     2161 ccaatttctc tgagtttctt gaaatgtctt taaaacagac atttttctca ccaaacagtt
     2221 tgagtatctt tattaaggaa acccttacga atcctgaaaa ttatgctagc atgatttttt
     2281 tatatataga agtttaaaaa taaaccaggt caggtttgta tatgtaaaat tgttgacatc
     2341 aatgatgtct ttccatattc ttatctgggc ttaagaaata cattctgtat ttttccagat
     2401 tctttgtagc ctttgaaaga tttttacagt acatatgtct tgactgagct gtcctttctt
     2461 aatacaaaag cgtgtataat tttcttaact tgtacagttg gtaaactttt atgagaggaa
     2521 ttgttattct gagtctgtca gctttcattt tattttgcta aggtttttct aatgaatttt
     2581 taagtgtttg tgtagtaatt aagtcatatt tcttatccag gtggttaaag cattcataaa
     2641 ggattataaa attttttttt tttttttttt ttttaaattt ctacttaagg gcaagtaatt
     2701 tgagaattct gaaattaagc atgatgctta gattgcagtt tgttttgcat ttgctataat
     2761 tttcaaaatt atttttgaag caagacaaaa gtttaatgtc agcttaagtg cttaaatata
     2821 tcatgctgac cagaatcctg ttatttttat atgcatcata aaatttccca tttctgcatt
     2881 aaataaacag aggagggggg tcaagtgata cttagatatt ggcacacaca aggaacaact
     2941 gttggcaggc agtatcgatt ttatgaagag aaagtgaagt ttgaaaactt ttcaaacttt
     3001 tctagttaca atccaatttt tcagatttga taatgctttt ccaaagtgaa aataagatac
     3061 acaataatca ttgctctgtg tgattacaga taggattatc catctgcata gccttgtaaa
     3121 agtgccattt tatttttagt gctaaattct tgttaaataa tgtagtttta tatagctgat
     3181 agaccaacct atatccaaac ttttataaaa tattaattat tcttgttttt ctcacacagg
     3241 catactccaa atgcttcttc cagttcattt ttcagccatc agttcaagag ccaatgcctt
     3301 tttaaaataa agcttctgtg gtcttgtttt taatggctca actgtctgat gtaattgagt
     3361 gaaggtttgc actgagaaat tagcattcag gccttacccc catgaagtat tactgttaac
     3421 atatgttcgg actgcttccc ttcaccaatg tgaacaactt tttttcccaa acagtgttaa
     3481 aagccacttt gcaacacttg acttcatctt aatgtacatt cactgttgtt acatacatat
     3541 ctaagtaaat caaagttttg ggtggaagtg ttgagaagta tgagtttttt gttgtttttg
     3601 ttttacttaa aacttttaat ttatccagaa tggcagtagc tttagcaagc agatggtcac
     3661 aatctgtttt ctaaatcatt ttttattaaa tgaatccaaa gtatcctagc tctttgtcaa
     3721 agatggtctg tagaaatgtt tttaaagatt agggatgctg tactttttga attgttgcag
     3781 tgttggagat gccattttca cctttttaaa aagatgcatt ttatttgcag gtttgtgaag
     3841 ttcttgcaaa ctcttactac agaaatgttt taaatacatt ctcaggcatg tttgcaaaaa
     3901 tatggcacat gtatacattt taggaaatgt tacttttgtt aatctttaaa aaatacacct
     3961 aatgaaagac attccactat tatgcgtaat gctcagcttt ttgtaaagaa atatttaatt
     4021 gtattttgtg tttatacagg tatttcacac aataatttgt tcttaatgca gccactataa
     4081 cttgataagt cattgcacta tttaaaaagt tttagtaata tcatgaattt aatttgctta
     4141 agatttagta catttcagaa cttttgaact tttgacaaat tgcattggaa aaagaagtct
     4201 gttaatagtg atttagtctc cccgcctcat tcccaccccc aacaaagcac tatcattttc
     4261 atttggaatg ggtattttct gtttcatgat gtattttcaa atagagttca ggatctgcaa
     4321 ttcagttcaa cacaagtttg ttgagcttta aaatgtattt gaagtaatat ttaaataggc
     4381 atttaaaatt tcaaattaga tgttaaattt atgcatagta attttgcact gtaaaataat
     4441 tcccttctcc cattccttgt ctgccattct gaatatgctt attaaaatat ttttttaaac
     4501 atatttgcct acataatact gtgtgaatat tttcattaga ttaattcact aagattttat
     4561 tagagattgt ttgaatgatg catgttattg acaaggcaaa tatatatata tacacagtct
     4621 gtttcttcta ttcctagaca tattgcacta ctttttcagt ggttcttggt cccctttacc
     4681 acatcttggg tgatgtattt ttgtcttctt cccctttgcc acaagagatc ctttcagtcc
     4741 ctagacctcc attcactctg tttctcttct gctggattat ataatttaaa aataatgtgt
     4801 tcccgtattt tgtagtggag ttcatatcac attatgtttt gcaaaataaa acttgttttg
     4861 tcacaatatt ttctttgcta tcgatggata attaggagtg ggcacttgga ataactgagg
     4921 gctggtgaat ataataggca atatttacat ggggtgtgta actaaatacc aaatcagata
     4981 gtaaggtttt cccaactata atccatattc atgaaaaatg ccagtgtgga aaaccatgta
     5041 acatacagaa gaacaggagt ttccaaattt taacctttta ttctaaaaat gactctcttc
     5101 tctcaaaatg actttttctg tatcacataa ttctactgat acaacttttt accgtaaaaa
     5161 ttaactctct tctttaatat caagttcatc ctttgcagaa tcttaaaggg ttttccacac
     5221 atccatccac ccacttacag actgatttca tgtatctgaa tatctgatta acctttcttt
     5281 tctaatacca gttttccata aactcttgtc atgtaccctt agtattgtgc tgatcttttg
     5341 ccaactatac ggacgtggag tttttccttt tcagaatatg attattttca atttgtcttt
     5401 agttagtata aagaattata gaatgtataa tgtaattaaa atgtcatttt gcttgc
//
