LOCUS       NM_019008               5688 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_019008
VERSION     NM_019008.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Gao,T., Shi,R., Liu,Z., De,D., Li,R., Chen,Y., Pei,J. and Ding,M.
  TITLE     Ischemia/reperfusion-induced MiD51 upregulation recruits Drp1 to
            mitochondria and contributes to myocardial injury
  JOURNAL   Biochem Biophys Res Commun 665, 78-87 (2023)
   PUBMED   37149986
  REMARK    GeneRIF: Ischemia/reperfusion-induced MiD51 upregulation recruits
            Drp1 to mitochondria and contributes to myocardial injury.
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Wong,Y.C., Kim,S., Cisneros,J., Molakal,C.G., Song,P., Lubbe,S.J.
            and Krainc,D.
  TITLE     Mid51/Fis1 mitochondrial oligomerization complex drives lysosomal
            untethering and network dynamics
  JOURNAL   J Cell Biol 221 (10) (2022)
   PUBMED   36044022
  REMARK    GeneRIF: Mid51/Fis1 mitochondrial oligomerization complex drives
            lysosomal untethering and network dynamics.
REFERENCE   3  (bases 1 to 5688)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 5688)
  AUTHORS   Charif,M., Wong,Y.C., Kim,S., Guichet,A., Vignal,C., Zanlonghi,X.,
            Bensaid,P., Procaccio,V., Bonneau,D., Amati-Bonneau,P., Reynier,P.,
            Krainc,D. and Lenaers,G.
  TITLE     Dominant mutations in MIEF1 affect mitochondrial dynamics and cause
            a singular late onset optic neuropathy
  JOURNAL   Mol Neurodegener 16 (1), 12 (2021)
   PUBMED   33632269
  REMARK    GeneRIF: Dominant mutations in MIEF1 affect mitochondrial dynamics
            and cause a singular late onset optic neuropathy.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5688)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 5688)
  AUTHORS   Andreev,D.E., O'Connor,P.B., Fahey,C., Kenny,E.M., Terenin,I.M.,
            Dmitriev,S.E., Cormican,P., Morris,D.W., Shatsky,I.N. and
            Baranov,P.V.
  TITLE     Translation of 5' leaders is pervasive in genes resistant to eIF2
            repression
  JOURNAL   Elife 4, e03971 (2015)
   PUBMED   25621764
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5688)
  AUTHORS   Koirala,S., Guo,Q., Kalia,R., Bui,H.T., Eckert,D.M., Frost,A. and
            Shaw,J.M.
  TITLE     Interchangeable adaptors regulate mitochondrial dynamin assembly
            for membrane scission
  JOURNAL   Proc Natl Acad Sci U S A 110 (15), E1342-E1351 (2013)
   PUBMED   23530241
REFERENCE   8  (bases 1 to 5688)
  AUTHORS   Loson,O.C., Song,Z., Chen,H. and Chan,D.C.
  TITLE     Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in
            mitochondrial fission
  JOURNAL   Mol Biol Cell 24 (5), 659-667 (2013)
   PUBMED   23283981
  REMARK    GeneRIF: we find that either MiD49 or MiD51 can mediate Drp1
            recruitment and mitochondrial fission in the absence of Fis1 and
            Mff
REFERENCE   9  (bases 1 to 5688)
  AUTHORS   Zhao,J., Liu,T., Jin,S., Wang,X., Qu,M., Uhlen,P., Tomilin,N.,
            Shupliakov,O., Lendahl,U. and Nister,M.
  TITLE     Human MIEF1 recruits Drp1 to mitochondrial outer membranes and
            promotes mitochondrial fusion rather than fission
  JOURNAL   EMBO J 30 (14), 2762-2778 (2011)
   PUBMED   21701560
  REMARK    GeneRIF: elevated MIEF1 levels induce mitochondrial fusion;
            depletion of MIEF1 causes mitochondrial fragmentation; MIEF1
            interacts with and recruits Drp1 to mitochondria but inhibits Drp1
            activity, executing a negative effect on mitochondrial fission
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 5688)
  AUTHORS   Palmer,C.S., Osellame,L.D., Laine,D., Koutsopoulos,O.S.,
            Frazier,A.E. and Ryan,M.T.
  TITLE     MiD49 and MiD51, new components of the mitochondrial fission
            machinery
  JOURNAL   EMBO Rep 12 (6), 565-573 (2011)
   PUBMED   21508961
  REMARK    GeneRIF: MiD49/51 are new mediators of mitochondrial division
            affecting Drp1 action at mitochondria.
            GeneRIF: Mitochondrial outer membrane protein that recruits fission
            mediator Drp1 to the mitochondrial surface.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL022312.7.
            
            On Oct 27, 2020 this sequence version replaced NM_019008.5.
            
            Transcript Variant: This variant (1) represents a predominant
            transcript, which encodes a 463aa protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853567.20044.1, AL365515.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 21508961; reported by
                                                  MitoCarta
            MANE Ensembl match                 :: ENST00000325301.7/
                                                  ENSP00000327124.2
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            regulatory uORF                    :: PMID: 25621764
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               AL022312.7         42979-43126
            149-480             AL022312.7         44892-45223
            481-631             AL022312.7         51977-52127
            632-809             AL022312.7         52538-52715
            810-1072            AL022312.7         52921-53183
            1073-5688           AL022312.7         54206-58821
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.1"
     gene            1..5688
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /note="mitochondrial elongation factor 1"
                     /db_xref="GeneID:54471"
                     /db_xref="HGNC:HGNC:25979"
                     /db_xref="MIM:615497"
     exon            1..148
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /inference="alignment:Splign:2.1.0"
     exon            149..480
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      177..389
                     /regulatory_class="uORF"
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:25621764]"
                     /note="this uORF is predicted to encode a 70 aa peptide
                     (PMID:25621764)"
     exon            481..631
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /inference="alignment:Splign:2.1.0"
     CDS             488..1879
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /note="isoform 1 is encoded by transcript variant 1;
                     smith-Magenis syndrome chromosomal region candidate gene 7
                     protein-like; mitochondrial dynamic protein of 51 kDa;
                     mitochondrial dynamic protein MID51; alternative protein
                     SMCR7L; Smith-Magenis syndrome chromosome region,
                     candidate 7-like; SMCR7-like protein; mitochondrial
                     dynamics protein of 51 kDa; mitochondrial dynamics protein
                     MID51; alternative MIEF1 protein; MIEF1 microprotein;
                     mitochondrial dynamics protein MIEF1; DANGER family member
                     3A"
                     /codon_start=1
                     /product="mitochondrial dynamics protein MIEF1 isoform 1"
                     /protein_id="NP_061881.2"
                     /db_xref="CCDS:CCDS13995.1"
                     /db_xref="GeneID:54471"
                     /db_xref="HGNC:HGNC:25979"
                     /db_xref="MIM:615497"
                     /translation="MAGAGERKGKKDDNGIGTAIDFVLSNARLVLGVGGAAMLGIATL
                     AVKRMYDRAISAPTSPTRLSHSGKRSWEEPNWMGSPRLLNRDMKTGLSRSLQTLPTDS
                     STFDTDTFCPPRPKPVARKGQVDLKKSRLRMSLQEKLLTYYRNRAAIPAGEQARAKQA
                     AVDICAELRSFLRAKLPDMPLRDMYLSGSLYDDLQVVTADHIQLIVPLVLEQNLWSCI
                     PGEDTIMNVPGFFLVRRENPEYFPRGSSYWDRCVVGGYLSPKTVADTFEKVVAGSINW
                     PAIGSLLDYVIRPAPPPEALTLEVQYERDKHLFIDFLPSVTLGDTVLVAKPHRLAQYD
                     NLWRLSLRPAETARLRALDQADSGCRSLCLKILKAICKSTPALGHLTASQLTNVILHL
                     AQEEADWSPDMLADRFLQALRGLISYLEAGVLPSALNPKVNLFAELTPEEIDELGYTL
                     YCSLSEPEVLLQT"
     exon            632..809
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /inference="alignment:Splign:2.1.0"
     exon            810..1072
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..5688
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4813..4818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /note="hexamer: AATAAA"
     polyA_site      4830
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /note="major polyA site"
     regulatory      5667..5672
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /note="hexamer: AATAAA"
     polyA_site      5688
                     /gene="MIEF1"
                     /gene_synonym="AltMIEF1; D3A; dJ1104E15.3; HSU79252;
                     L0R8F8; MID51; MIEF1-MP; OPA14; SMCR7L"
                     /note="major polyA site"
ORIGIN      
        1 actcgccctc gtgctccctt cagccccttc gcagctccgt gcgcaaggtc gtgtcccgga
       61 agtgaagggg ccatgttgat gggtgacccg gggagaggta cccggccaga ggcgagtcct
      121 gcggagtggt agcgcgcacg gcctgcggtg tgacacccag cccctgccag tcccccatgg
      181 ccccgtggag ccgagaggcg gtgctgagtc tctatcgggc tctgttgcgc cagggccgac
      241 agcttcgcta cactgatcga gacttctact ttgcctccat ccgccgtgaa ttccgaaaaa
      301 atcagaagct agaggacgct gaggcccggg agaggcagct ggagaagggc ctggtctttc
      361 tcaacggcaa attggggagg atcatttagg atcctccaag ggaaagagga caaaggtgcc
      421 ttctgtagac actcctgctc tcttccatcc ccatcttaca gatgtattaa gaagcctcag
      481 atgagcaatg gcaggcgctg gtgagcgcaa aggcaagaag gatgacaatg gcattggcac
      541 ggccattgac tttgtgctct ccaatgcccg gctggtgctg ggggtgggtg gagcggccat
      601 gctgggcatc gccacgctgg cagttaagcg gatgtacgat cgggcgatca gtgcccctac
      661 cagccccacc cgcctgagcc attcggggaa aaggagctgg gaagaaccca actggatggg
      721 ctccccacga ctgctgaaca gggacatgaa gacgggcctg agccggtcct tgcagaccct
      781 tcccacagac tcctccacct tcgacacaga tacattctgc ccgccccggc ccaagccagt
      841 ggccaggaag ggccaggtag acttgaagaa gtcacgactc cgcatgtccc tgcaggagaa
      901 acttcttact tactaccgga accgggcagc catccctgct ggagagcagg ctcgggccaa
      961 gcaagctgct gtggacatat gtgccgagct ccggagcttc ctgcgggcca agttgcctga
     1021 catgccgctt cgggacatgt acttgagtgg cagcctctac gatgacctgc aggtggtgac
     1081 agctgaccac atccaactca ttgtgcccct tgtgctggag cagaacctgt ggtcatgtat
     1141 tcctggtgaa gacaccatca tgaatgtccc tggcttcttc ctggtgcgtc gtgagaatcc
     1201 agagtacttt cctcgtggga gcagttactg ggaccgctgt gtagtagggg gctacctctc
     1261 tccaaagaca gtcgcagata catttgagaa ggtagtggct ggctccatca attggccagc
     1321 catagggtcc ctcttggact atgtgatccg cccggcccca cccccagaag ccctcacact
     1381 ggaggtgcag tatgagcgtg acaaacatct cttcattgac ttcctgccat cagtgaccct
     1441 cggtgacaca gtcttggtgg ccaaaccaca ccggctagcc cagtatgaca acctgtggcg
     1501 gctgagcctg cgtcccgcgg agacggcacg cctgcgggct ctggaccagg ctgactcggg
     1561 ctgccgatct ctgtgcctca agatcctcaa ggccatatgc aagtccaccc cggctctggg
     1621 ccacctcact gccagccagc taaccaatgt catcctccac ttggcccagg aggaggctga
     1681 ctggtctccg gatatgctgg ccgaccgttt cctgcaggcc ttgaggggac ttatcagcta
     1741 cttagaggct ggagtcctgc ccagtgccct aaaccccaag gtgaacttat ttgcagagct
     1801 cacccctgaa gaaatagacg aattaggata cactctgtat tgctcattgt ctgagccaga
     1861 ggtgctgctg cagacgtagg gcaggtgaag gccaaagcgg gtgttggtgg tcaggccctg
     1921 gattctccgt tagatacact tggctaccta gttggtgcct cacagggttc ctgctgcctg
     1981 gtgtcttgct gatcatcacc ctggtcactt catgctgatt agaatgacat ctctttcgtc
     2041 tcctattttg ttacccaact cttcctattt ttgttaccaa tcactgtgct ctctgccgcc
     2101 ccctggctcc aggctaattt ttctggaatg aattgagaag gtggcgtgct ggcctgagct
     2161 gatggaccac ttggtgtttt gcgttttggc ccatgtttgc tgcctctatc tggtctgcct
     2221 tgcccgtttg cctgttccta ttcagtgtct tttctatttt ttcctctctc gttcatgcct
     2281 tctgttttgc tcttgtccct ggagcatatc tgcctaatta agatgttgcc ttttagttga
     2341 atgccactga agagctgtga tagcatgttt caaagctgaa ctctacagag cgagtgctga
     2401 gacagtattt agggtttctg ggagtgaggc tggtagaaga gttggccttt gaccacggtt
     2461 cctggagtag aagtccatcc tccccccaac ctcctgaccc attcataaat gctgagaatg
     2521 tctctcatgg gaacactgtt aatgacccac acaggataag ctgaatgcaa agttatttgc
     2581 aggttgaatt tcttggtggc tattagcaga agtgcagagt agggaaccag agctggttaa
     2641 gggcctagtg aagggtttgt gtgcccagtg tctgctcgtc atctgtggct gcaggggtca
     2701 gacagacaag gatggggact gccagggcac cacttcatca tgaatgctgg ttttcacacc
     2761 ttttccttat tttattgcca atcaggacaa ggccttgaag gaacgcagcc ttagacatca
     2821 ggtgaggatg atggaggtag acagtcgact gaatgtcagc tggaaaatcc agtcactagt
     2881 tggggtttgg tggccatgtt ttctacccag acaggccctg cttttctagg atgtggcctt
     2941 agagcaagaa cagacccaac agccagccct tcatcctcca gcgtctgcca taggaatgtg
     3001 agaggggtgt ttgctgagcg ctccgggcac ggccagaggg caagtgagca tgcacggacc
     3061 tcttccccct gtcctgtttc tcacccagca cctggggaga tcggtgctac caaggaagag
     3121 agcacacaga taagacagag gggaggaggt gggcatttcc tacattcctc cttgtttgcc
     3181 gctgctgaga ttgcagtatt tattgcaatg taaatgtatc ctgaaggtgg ggaggaatgt
     3241 ttaatctacc atgtccgtgt gtcatcttgg tttgtgtttt tccctgtttg tagcaagact
     3301 ctgatgataa ttctgtttct catctgccca ttcagtattt tgttttcctt ccgtcaagtt
     3361 gtcttatttt ttcaatgact acctctccat cattgaggtt ctggtgaagc tctctgcagc
     3421 tgtctcattc cttcccaacg atagtaacag gaaatgactc tttagcatcg atacctcaac
     3481 atcaatttag ggtagagatt cctgcccctc ttttgtcaca gattaggaaa ttgagaacta
     3541 gggttaacct tgactatatt tagaggtctt tttgcctctt ttccccttaa caaggatttc
     3601 ttatggtggt ttcagtttca tttgcataaa ggtattgaga gggaacaaaa aacataaagc
     3661 tgagaatctt gagagagctc atctaccctg tctgttggtc agactcaaat gagagttaaa
     3721 aaaaaaaaaa aaaatctgta tgcctgagta ccatcctgga tgaatctaga aggtatgggg
     3781 tagagcttga cagggttcct gtgtacccac tgggtatccg ttagaggtaa gggagaggag
     3841 aggattgata gagtgttgca aaagtataga ttattcattg agataaagga tttggtttcc
     3901 ctgccatgag tattaaaaaa atttaagttt tcccaagctt gcatctctga ccaaatttca
     3961 cataaaacat tggaaggagg ctgggtgcgg tggctcatgc ttgtaatccc agcactggga
     4021 agctaaggcg ggtggatcac ttgaggtcag gagttcgaga ccagcctggc caacatggtg
     4081 aaaccccgtc tccacgaaaa agataaaaat aagctgggcg tggtggcagg cgcctataat
     4141 cccagctact cgggaggctg aggcaggaga ataacttaaa cccgggaggc ggaggttaca
     4201 gtgagctgag atcgtgccac tgcactccag cctgggtgac agagtgagac cctatttcaa
     4261 aaaaataaaa attggaagaa gagcttaaaa aagataagat tttaaagagt cccaagttat
     4321 ttaagttgag tgtaattgtc atttaaggaa ggcaaatgag tttatcatcc ttcttaaaga
     4381 gcatctcttt taactgttgg acaaaaccat aactttgtca ttttacaagg aagaacctct
     4441 taagaagtcc tcagaaccag aagcaatgtg aactctcagc gctggtcctg gtgggtttgc
     4501 tgaccatgac tgggcaagcc gttctttttg ctgccatctt cctcatcata aagtgtggaa
     4561 cataggcaat tgctttgaga ttcttggata gaagaggaca acattctgca cctgccccct
     4621 tttttaaatc tttggggaaa gatgagtaac tttccccact actctgcctt cctgttcagt
     4681 aactcttact tttgcctgaa gtaacagcat cttctacttc tccatctaga gatttttgtg
     4741 tgtgtgccat caaggttagc aaactttata cgtagcctaa cacttaaaaa atgcactcat
     4801 tatcttaaac ctaataaatt ccagagttta ttttggttct cctctgttgc ccttcctaaa
     4861 aaatgagctg aagatgacag tatttttctt tacatgcttg gttatgactt ttaaagtttt
     4921 atttaaataa atgttgaagc tcaagtttaa agaagcgttg cagaggccca cggtctcctg
     4981 ggtcccggcc acctgtccat attccacatt tgctgactgt gctccctgca ctccactcaa
     5041 gttgagagtt caaatagtct tgaaggggaa tcagcttcag gatggaagga cccaggagag
     5101 gccccgaggt gggagggttc tgtaaataca gactactgcg agtgtccaga gctctctgcc
     5161 atgatacttc cttgggactg acttggctga gaacgtgttc tgtcagagga tttgttagaa
     5221 ctctgccctt ttgtctgaaa ctcaaggcca aggagaatga taggagactt aggacagagc
     5281 tgacccttgc accaggctgg gaggctgcag cccttttaga tgccacttac tgtaagtggc
     5341 cagaatacca gagaggtggg ttccatggtc aaatgcacag taggtgttta cctttacatt
     5401 tggatcacct tgtagtcttt aaattcttgg tccctgaggc caagtccaca acttgccttc
     5461 tagtcacttg cctgcccgca gtggtggtgg atgtgttagc tggtagattt ggaatcagtc
     5521 accagtcttt ctgtactgtc ttggttagct ctatataagt aggggcagct tagccctgag
     5581 gcccagagac ctgctgtcct ttttctcctt gagggaggaa ataaaactgc ggaatacaat
     5641 gtccttccat agcatgggaa gaagaaaata aacatctcct ttccaaca
//
