LOCUS       NM_000381               6168 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens midline 1 (MID1), transcript variant 1, mRNA.
ACCESSION   NM_000381
VERSION     NM_000381.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Lin,L., Huang,Z., Li,W., Liu,X., Li,X., Gao,S., Chen,J., Yang,C.,
            Min,X., Yang,H., Gong,Q., Wei,Y., Tu,S., Rao,X., Zhang,Z., Dong,L.
            and Zhong,J.
  TITLE     Mid1 promotes synovitis in rheumatoid arthritis via
            ubiquitin-dependent post-translational modification
  JOURNAL   Pharmacol Res 205, 107224 (2024)
   PUBMED   38777113
  REMARK    GeneRIF: Mid1 promotes synovitis in rheumatoid arthritis via
            ubiquitin-dependent post-translational modification.
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Wang,Y.
  TITLE     Erdr1 Drives Macrophage Programming via Dynamic Interplay with YAP1
            and Mid1
  JOURNAL   Immunohorizons 8 (2), 198-213 (2024)
   PUBMED   38392560
  REMARK    GeneRIF: Erdr1 Drives Macrophage Programming via Dynamic Interplay
            with YAP1 and Mid1.
REFERENCE   3  (bases 1 to 6168)
  AUTHORS   Frank,S., Gabassi,E., Kaseberg,S., Bertin,M., Zografidou,L.,
            Pfeiffer,D., Brennenstuhl,H., Falk,S., Karow,M. and Schweiger,S.
  TITLE     Absence of the RING domain in MID1 results in patterning defects in
            the developing human brain
  JOURNAL   Life Sci Alliance 7 (4), e202302288 (2024)
   PUBMED   38238086
  REMARK    GeneRIF: Absence of the RING domain in MID1 results in patterning
            defects in the developing human brain.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6168)
  AUTHORS   Perea-Cabrera,M., Granados-Riveron,J.T., Segura-Stanford,B.,
            Moreno-Vargas,L.M., Prada-Gracia,D., Moran-Espinosa,M.C.,
            Erdmenger,J., Diaz-Garcia,H. and Sanchez-Urbina,R.
  TITLE     Opitz GBBB syndrome with total anomalous pulmonary venous
            connection: A new MID1 gene variant
  JOURNAL   Mol Genet Genomic Med 11 (9), e2234 (2023)
   PUBMED   37498300
  REMARK    GeneRIF: Opitz GBBB syndrome with total anomalous pulmonary venous
            connection: A new MID1 gene variant.
REFERENCE   5  (bases 1 to 6168)
  AUTHORS   Gaudenz,K., Roessler,E., Quaderi,N., Franco,B., Feldman,G.,
            Gasser,D.L., Wittwer,B., Horst,J., Montini,E., Opitz,J.M.,
            Ballabio,A. and Muenke,M.
  TITLE     Opitz G/BBB syndrome in Xp22: mutations in the MID1 gene cluster in
            the carboxy-terminal domain
  JOURNAL   Am J Hum Genet 63 (3), 703-710 (1998)
   PUBMED   9718340
  REMARK    Erratum:[Am J Hum Genet 1998 Nov;63(5):1571]
REFERENCE   6  (bases 1 to 6168)
  AUTHORS   Perry,J., Feather,S., Smith,A., Palmer,S. and Ashworth,A.
  TITLE     The human FXY gene is located within Xp22.3: implications for
            evolution of the mammalian X chromosome
  JOURNAL   Hum Mol Genet 7 (2), 299-305 (1998)
   PUBMED   9425238
REFERENCE   7  (bases 1 to 6168)
  AUTHORS   Quaderi,N.A., Schweiger,S., Gaudenz,K., Franco,B., Rugarli,E.I.,
            Berger,W., Feldman,G.J., Volta,M., Andolfi,G., Gilgenkrantz,S.,
            Marion,R.W., Hennekam,R.C., Opitz,J.M., Muenke,M., Ropers,H.H. and
            Ballabio,A.
  TITLE     Opitz G/BBB syndrome, a defect of midline development, is due to
            mutations in a new RING finger gene on Xp22
  JOURNAL   Nat Genet 17 (3), 285-291 (1997)
   PUBMED   9354791
REFERENCE   8  (bases 1 to 6168)
  AUTHORS   Robin,N.H., Feldman,G.J., Aronson,A.L., Mitchell,H.F., Weksberg,R.,
            Leonard,C.O., Burton,B.K., Josephson,K.D., Laxova,R., Aleck,K.A.,
            Allanson,J.E., Guion-Almeida,M.L., Martin,R.A., Leichtman,L.G.,
            Price,R.A., Opitz,J.M. and Muenke,M.
  TITLE     Opitz syndrome is genetically heterogeneous, with one locus on
            Xp22, and a second locus on 22q11.2
  JOURNAL   Nat Genet 11 (4), 459-461 (1995)
   PUBMED   7493033
REFERENCE   9  (bases 1 to 6168)
  AUTHORS   Scott,D.A.
  TITLE     Esophageal Atresia / Tracheoesophageal Fistula Overview - RETIRED
            CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301753
REFERENCE   10 (bases 1 to 6168)
  AUTHORS   Meroni,G.
  TITLE     MID1-Related Opitz G/BBB Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301502
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC309977.1, BC053626.1 and
            EF217426.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 1, 2019 this sequence version replaced NM_000381.3.
            
            Summary: The protein encoded by this gene is a member of the
            tripartite motif (TRIM) family, also known as the 'RING-B
            box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM
            motif includes three zinc-binding domains, a RING, a B-box type 1
            and a B-box type 2, and a coiled-coil region. This protein forms
            homodimers which associate with microtubules in the cytoplasm. The
            protein is likely involved in the formation of multiprotein
            structures acting as anchor points to microtubules. Mutations in
            this gene have been associated with the X-linked form of Opitz
            syndrome, which is characterized by midline abnormalities such as
            cleft lip, laryngeal cleft, heart defects, hypospadias, and
            agenesis of the corpus callosum. This gene was also the first
            example of a gene subject to X inactivation in human while escaping
            it in mouse. Alternative promoter use, alternative splicing and
            alternative polyadenylation result in multiple transcript variants
            that have different tissue specificities. [provided by RefSeq, Dec
            2016].
            
            Transcript Variant: This variant (1, also known as alpha) is the
            longest transcript. Variants 1-4 and variant 10 encode the same
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.16033.1,
                                           SRR1660807.78880.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000317552.9/ ENSP00000312678.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-459               DC309977.1         50-508
            460-3615            BC053626.1         366-3521
            3616-6168           EF217426.1         1261-3813
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.2"
     gene            1..6168
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /note="midline 1"
                     /db_xref="GeneID:4281"
                     /db_xref="HGNC:HGNC:7095"
                     /db_xref="MIM:300552"
     exon            1..296
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     exon            297..1012
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     CDS             353..2356
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     putative transcription factor XPRF; tripartite motif
                     protein TRIM18; zinc finger on X and Y, mouse, homolog of;
                     tripartite motif-containing protein 18; RING finger
                     protein 59; midline 1 RING finger protein; RING finger
                     protein Midline-1; E3 ubiquitin-protein ligase Midline-1;
                     Opitz/BBB syndrome; RING-type E3 ubiquitin transferase
                     Midline-1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Midline-1 isoform 1"
                     /protein_id="NP_000372.1"
                     /db_xref="CCDS:CCDS14138.1"
                     /db_xref="GeneID:4281"
                     /db_xref="HGNC:HGNC:7095"
                     /db_xref="MIM:300552"
                     /translation="METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCAT
                     NESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRR
                     ERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHR
                     LIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDK
                     LKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQR
                     RQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKN
                     ITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELC
                     TASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTV
                     HGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVER
                     DESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHE
                     WIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSI
                     HLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP"
     exon            1013..1108
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1216
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1365
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1493
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1637
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1799
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..2007
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..6168
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6142..6147
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /note="hexamer: AATAAA"
     polyA_site      6168
                     /gene="MID1"
                     /gene_synonym="BBBG1; FXY; GBBB; GBBB1; MIDIN; OGS1; OS;
                     OSX; RNF59; TRIM18; XPRF; ZNFXY"
                     /note="major polyA site"
ORIGIN      
        1 gagaactgat ttgaaataag agccaggcgg tcctcgcttc cctgcgaccg acttttcata
       61 ggctggggga gaaaaggttg agaaacttga cattgtctcg agcagagtgc gtgtagcaac
      121 agatcaaaga aaaagaggac gaaaacgtgc tctttgctgc ccgtagattt cgccgggttg
      181 cttttgtctt gcgggctcct gtcgggttcg gtgtttccgc tctgaagact gcgacgcggg
      241 ctccgatgca gctcgctccc tgccggatgg gtcatgggat tctaaacatg aggcagatag
      301 ctgatcagct tccttgggtt ttgctgatga cacaagagag ctttgcctga agatggaaac
      361 actggagtca gaactgacct gccctatttg tctggagctc tttgaggacc ctcttctact
      421 gccctgcgca cacagcctct gcttcaactg cgcccaccgc atcctagtat cacactgtgc
      481 caccaacgag tctgtggagt ccatcaccgc cttccagtgc cccacctgcc ggcatgtcat
      541 caccctcagc cagcgaggtc tagacgggct caagcgcaac gtcaccctac agaacatcat
      601 cgacaggttc cagaaagcat cagtgagcgg gcccaactct cccagcgaga cccgtcggga
      661 gcgggccttt gacgccaaca ccatgacctc cgccgagaag gtcctctgcc agttttgtga
      721 ccaggatcct gcccaggacg ctgtgaagac ctgtgtcact tgtgaagtat cctactgtga
      781 cgagtgcctg aaagccactc acccgaataa gaagcccttt acaggccatc gtctgattga
      841 gccaattccg gactctcaca tccgggggct gatgtgcttg gagcatgagg atgagaaggt
      901 gaatatgtac tgtgtgaccg atgaccagtt aatctgtgcc ttgtgtaaac tggttgggcg
      961 gcaccgcgat catcaggtgg cagctttgag tgagcgctat gacaaattga agcaaaactt
     1021 agagagtaac ctcaccaacc ttattaagag gaacacagaa ctggagaccc ttttggctaa
     1081 actcatccaa acctgtcaac atgttgaagt caatgcatca cgtcaagaag ccaaattgac
     1141 agaggagtgt gatcttctca ttgagatcat tcagcaaaga cgacagatta ttggaaccaa
     1201 gatcaaagaa gggaaggtga tgaggcttcg caaactggct cagcagattg caaactgcaa
     1261 acagtgcatt gagcggtcag catcactcat ctcccaagcg gaacactctc tgaaggagaa
     1321 tgatcatgcg cgtttcctac agactgctaa gaatatcacc gagagagtct ccatggcaac
     1381 tgcatcctcc caggttctaa ttcctgaaat caacctcaat gacacatttg acacctttgc
     1441 cttagatttt tcccgagaga agaaactgct agaatgtctg gattacctta cagctcccaa
     1501 ccctcccaca attagagaag agctctgcac agcttcatat gacaccatca ctgtgcattg
     1561 gacctccgat gatgagttca gcgtggtctc ctacgagctc cagtacacca tattcaccgg
     1621 acaagccaac gtcgttagtc tgtgtaattc ggctgatagc tggatgatag tacccaacat
     1681 caagcagaac cactacacgg tgcacggtct gcagagcggc accaagtaca tcttcatggt
     1741 caaggccatc aaccaggcgg gcagccgcag cagtgagcct gggaagttga agacaaacag
     1801 ccaaccattt aaactggatc ccaaatctgc tcatcgaaaa ctgaaggtgt cccatgataa
     1861 cttgacagta gaacgtgatg agtcatcatc caagaagagt cacacacctg aacgcttcac
     1921 cagccagggg agctatggag tagctggaaa tgtgtttatt gatagtggcc ggcattattg
     1981 ggaagtggtc ataagtggaa gcacatggta tgccattggt cttgcttaca aatcagcccc
     2041 gaagcatgaa tggattggga agaactctgc ttcctgggcg ctctgccgct gcaacaataa
     2101 ctgggtggtg agacacaata gcaaggaaat ccccattgag cctgcccccc acctccggcg
     2161 cgtgggcatc ctgctggact atgataacgg ctctatcgcc ttttatgatg ctttgaactc
     2221 catccacctc tacaccttcg acgtcgcatt tgcgcagcct gtttgcccca ccttcaccgt
     2281 gtggaacaag tgtctgacga ttatcactgg gctccctatc ccagaccatt tggactgcac
     2341 agagcagctg ccgtgagcgt ctggccacat ggagctgctt tctggggaac agtaaggttc
     2401 aggccactat ttaggggact gagaaagcac aggcttcatg agtgtaatga aatctcacca
     2461 gaagtgtccc gaaatcggct cagatagggc tcaaaacaag agattcctct ccttttactg
     2521 tgtcttgtat taagtacggg ctttaataat ttctttaatt tttttgtatt tagaggaaaa
     2581 tctatagatt atttataaga gaaacataat caggattaca acttttagga attacttggt
     2641 tttgcacatt aagaggccca taagtttatc agctatttac aaccttcatt tcatcacaat
     2701 ctgtgggctt acaaaaaaac aaaaactttt gtagttttgt atgttactca tcttcttacc
     2761 tgatatccca tgatgatccc atggtaggtc ttctcacctc gatggtgcat aacaggatgt
     2821 gtttgaacct agtaggggag gaaacaggct ttcttactct ggtttaattt gaagtgtttt
     2881 aattgtgatg tcaaaaagtt gtatcagatc aactaaaatg gagagcaaga cagagaatga
     2941 aaagagttga ttttggacct cggaccttgc cgtggctaaa tctttacctt ctcatagctg
     3001 atgggataat gttggaaaga aaggttctga atcctttggc cacattttgc cctgcttctc
     3061 tcagggttaa gggttctgga agaacattaa gaatgagatt gcaattgaaa atagtcattt
     3121 tgaatcctat tgattattca aaaattcagg ctgatttgtc ttttatcaga ggtaggattc
     3181 tgttttatag tatagaatct actttatcct tccttttaat agttccttta gacctgtgaa
     3241 atttcttcac tacatttaat agttctccta tttcccgctc ccccatatca attttccttt
     3301 tgtctccggg gctgagtaaa taaacatgtt ctgtcacaaa tagcagcacc actttggatt
     3361 gattttgctc tccaggacat cagcacatgg ccctgatcag cactaccaca tccaaacata
     3421 agtcactgaa aaacacttaa tatttatgag ttggtaatga caagggacat tgtataaagt
     3481 actatttgct agattcatgc ctcaaaagtt attataaaca gacctttatt aaacacatct
     3541 tgaaagatgt agaagtccct ctatagtcta gtatagttta caatagagtt gtaagaccaa
     3601 aaaaaaaaaa aaaaaattca aactcgttat tcaggaacct gcttataaaa tgtcagctgg
     3661 gattctttgc atgccaatct gatgcgttgg aatggtccat gaattaaggc tttcttgagc
     3721 agttcttggc ccagaactct ggcattggtt ctagtttgat gaagggcatg acctctataa
     3781 atggtttcaa ttgctaaaat atttacctgg gatactgggt cagccatttt gactgagcag
     3841 actagtggat ttagacattg tttttagtta ttttgttttt aaccaaatca accaactgcc
     3901 tccctgaaat aagtcaatga actcattgtt tcagcatcac gtggccaaag gtcatgtgat
     3961 tgcaaatctg gatttcaagg gaggccaagc cagcttcctg ggtccttcca tcctcttccc
     4021 tagcagacac tctccttttt cttaacagat agattctata tatttactat attatttata
     4081 cccagatgaa tattttgata gatacctaag acaatttcac atctaaaaga tggacgcctc
     4141 aatggaaaaa aaacaatctt tctctggaaa ccttataggt ttttcttttt attacaatat
     4201 aaaagcaatg tgtgtttgcc ttctctgagt aaactgaaag ggttgtctca gtaattttta
     4261 catacatttt gggtaactgg ataatggata ttttaatgca ctttgtacac taacaggttc
     4321 taaataaaag ggtctaaaac tcagcttctg agtttttaaa atcacggtct ccaggtacca
     4381 ataaatgcta cagtttgcct tatgatgtta acataaaaca cttagtagaa ggacaatatt
     4441 tccatgaaaa taatgttttt caatattaag aagttactac tcaaattttc acagtaagcc
     4501 atttagggta tgtttggcta tttttataag gacatgagag attatgtcat aattttgttg
     4561 tggaagtctc actcttggct aacttaaaag cattgtggat agtagcagtt actagttcca
     4621 ggttgtcata tttacaggaa aatatgtata tggtgaaagg ccaccgtgtt taattactat
     4681 aatgatgtag aaaagattcc cgtgtgaatt ttttttttga aagtctaaaa aatgtatgct
     4741 gtaaaaattt gctgcagtgt aattttgcat tctctttaaa ctgattgagg tcacagtatt
     4801 ttattatttg gggtcctcac cacaggaaac actgcgatac aggggcaaaa gagatggcag
     4861 tgccaattaa attaatacaa caaaatcaat gcagcaccaa ccaagactgc caggtctggt
     4921 gtcatgggta tgcccagagc ccaggagttc agaagggccc taagcctgat ttaatgctct
     4981 gctgttgatg tcttgaaatt cttaacaatt tttgaacaag gggcctgcgt tttcacttcg
     5041 cactgggcct tgcaaattac atagcgagtg ctcataaaag aactcagaaa cgtggtacct
     5101 ctcttcctgg tggatacaaa taaagaaatc tggatccaaa gttgaaagtt gctggcgata
     5161 tcattcaagt aggactctaa atagtggatt aagatgaggg tgggcctggg tgaagattct
     5221 ttccagcttt aaaagaaagt gacttcaaaa actgactgca aatattgacg atggtttctg
     5281 ctggaggaaa agaaacagct tgaatacaga caggcttttt tattacggta ctgatatatt
     5341 gaccttaaac ttgctgagga actgaactaa cgtcctccag tgaccgtgga attccatctc
     5401 agctccagga acatgcagat acctgcaaag agacacgcat atatgctggc atacatgtgc
     5461 atttggtgtt gggaagttga ccatctggtc tatcttaata aaatggtaaa aagcacacca
     5521 agacaatgat gggggcagga ggatgttttt gaaaacagcg cttctcaacc agtgctcgat
     5581 tttgcccccc aggagacatt tggcaatgca atggcaactt ttggttgtcg cagccgggga
     5641 agggaagcta ccagcatcta gtgcgtagag gtcatggacg ccgttaaaca tcctacagtg
     5701 caagcgcagc ccccgaccac gaagagttgt cttgctcaaa tatcaacagt gctgcagtgt
     5761 agaaacttga tcgttggttt tcttttaatg caaaactctc ataaaaacct ttcacttttc
     5821 ctgtcattga ttatatgctt gatacaccca aaaagaaaag gggaggggca ccaattcacc
     5881 tacactccag tggctccatc acctttaaaa atatttataa aatagttcca aaaatctgat
     5941 atctgaaaag caatccaagc ctgtgtaaat gggaatcact gataagtatc atcatctgta
     6001 tcagcttggc ttggacatga aaaattgatt ctctttatgt cactccttgc acctggacaa
     6061 attcaatccc cggtacttaa gtcacactgc caagccctcg gccctgacta ttgtcttgat
     6121 tgctgttcct ttctggttca aaataaaatc atttttgtgg caccaaga
//
