LOCUS       NM_015241               9447 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens microtubule associated monooxygenase, calponin and LIM
            domain containing 3 (MICAL3), transcript variant 1, mRNA.
ACCESSION   NM_015241 XM_032996 XM_943874
VERSION     NM_015241.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Kim,J., Lee,H., Roh,Y.J., Kim,H.U., Shin,D., Kim,S., Son,J.,
            Lee,A., Kim,M., Park,J., Hwang,S.Y., Kim,K., Lee,Y.K., Jung,H.S.,
            Hwang,K.Y. and Lee,B.C.
  TITLE     Structural and kinetic insights into flavin-containing
            monooxygenase and calponin-homology domains in human MICAL3
  JOURNAL   IUCrJ 7 (Pt 1), 90-99 (2020)
   PUBMED   31949908
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   Tominaga,K., Minato,H., Murayama,T., Sasahara,A., Nishimura,T.,
            Kiyokawa,E., Kanauchi,H., Shimizu,S., Sato,A., Nishioka,K.,
            Tsuji,E.I., Yano,M., Ogawa,T., Ishii,H., Mori,M., Akashi,K.,
            Okamoto,K., Tanabe,M., Tada,K.I., Tojo,A. and Gotoh,N.
  TITLE     Semaphorin signaling via MICAL3 induces symmetric cell division to
            expand breast cancer stem-like cells
  JOURNAL   Proc Natl Acad Sci U S A 116 (2), 625-630 (2019)
   PUBMED   30587593
  REMARK    GeneRIF: the niche factor Sema3-stimulated NP1/MICAL3/CRMP2/Numb
            axis appears to expand Cancer stem-like cells (CSCs) at least
            partly through increased frequency of MICAL3-mediated symmetric
            division of CSCs.
REFERENCE   3  (bases 1 to 9447)
  AUTHORS   Liu,Q., Liu,F., Yu,K.L., Tas,R., Grigoriev,I., Remmelzwaal,S.,
            Serra-Marques,A., Kapitein,L.C., Heck,A.J. and Akhmanova,A.
  TITLE     MICAL3 Flavoprotein Monooxygenase Forms a Complex with
            Centralspindlin and Regulates Cytokinesis
  JOURNAL   J Biol Chem 291 (39), 20617-20629 (2016)
   PUBMED   27528609
  REMARK    GeneRIF: MICAL3 acts as a midbody-associated scaffold for vesicle
            targeting, which promotes maturation of the intercellular bridge
            and abscission.
REFERENCE   4  (bases 1 to 9447)
  AUTHORS   Bachmann-Gagescu,R., Dona,M., Hetterschijt,L., Tonnaer,E.,
            Peters,T., de Vrieze,E., Mans,D.A., van Beersum,S.E., Phelps,I.G.,
            Arts,H.H., Keunen,J.E., Ueffing,M., Roepman,R., Boldt,K.,
            Doherty,D., Moens,C.B., Neuhauss,S.C., Kremer,H. and van Wijk,E.
  TITLE     The Ciliopathy Protein CC2D2A Associates with NINL and Functions in
            RAB8-MICAL3-Regulated Vesicle Trafficking
  JOURNAL   PLoS Genet 11 (10), e1005575 (2015)
   PUBMED   26485645
  REMARK    GeneRIF: Further exploration of the NINL-associated interactome
            identifies MICAL3, a protein known to interact with Rab8 and to
            play an important role in vesicle docking and fusion.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9447)
  AUTHORS   Li,C., Gonsalves,C.S., Eiymo Mwa Mpollo,M.S., Malik,P., Tahara,S.M.
            and Kalra,V.K.
  TITLE     MicroRNA 648 Targets ET-1 mRNA and is cotranscriptionally regulated
            with MICAL3 by PAX5
  JOURNAL   Mol Cell Biol 35 (3), 514-528 (2015)
   PUBMED   25403488
  REMARK    GeneRIF: a novel link wherein placenta growth factor-mediated
            downregulation of paired box protein 5 attenuates miR-648
            expression leading to increased endothelin-1 levels that are known
            to induce Pulmonary hypertension in sickle cell anemia
REFERENCE   6  (bases 1 to 9447)
  AUTHORS   Ong,B.A., Li,J., McDonough,J.M., Wei,Z., Kim,C., Chiavacci,R.,
            Mentch,F., Caboot,J.B., Spergel,J., Allen,J.L., Sleiman,P.M. and
            Hakonarson,H.
  TITLE     Gene network analysis in a pediatric cohort identifies novel lung
            function genes
  JOURNAL   PLoS One 8 (9), e72899 (2013)
   PUBMED   24023788
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9447)
  AUTHORS   Ratnayake,M., Reynard,L.N., Raine,E.V., Santibanez-Koref,M. and
            Loughlin,J.
  TITLE     Allelic expression analysis of the osteoarthritis susceptibility
            locus that maps to MICAL3
  JOURNAL   BMC Med Genet 13, 12 (2012)
   PUBMED   22385522
  REMARK    GeneRIF: data do not support our hypothesis that the association
            between rs2277831 and primary osteoarthriti is due to the effect
            this SNP has on MICAL3, BCL2L13 or BID gene expression
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 9447)
  AUTHORS   Grigoriev,I., Yu,K.L., Martinez-Sanchez,E., Serra-Marques,A.,
            Smal,I., Meijering,E., Demmers,J., Peranen,J., Pasterkamp,R.J., van
            der Sluijs,P., Hoogenraad,C.C. and Akhmanova,A.
  TITLE     Rab6, Rab8, and MICAL3 cooperate in controlling docking and fusion
            of exocytotic carriers
  JOURNAL   Curr Biol 21 (11), 967-974 (2011)
   PUBMED   21596566
  REMARK    GeneRIF: The monooxygenase activity of MICAL3 is required to
            regulate its own turnover and the concomitant remodeling of
            vesicle-docking protein complexes.
REFERENCE   9  (bases 1 to 9447)
  AUTHORS   Fischer,J., Weide,T. and Barnekow,A.
  TITLE     The MICAL proteins and rab1: a possible link to the cytoskeleton?
  JOURNAL   Biochem Biophys Res Commun 328 (2), 415-423 (2005)
   PUBMED   15694364
REFERENCE   10 (bases 1 to 9447)
  AUTHORS   Terman,J.R., Mao,T., Pasterkamp,R.J., Yu,H.H. and Kolodkin,A.L.
  TITLE     MICALs, a family of conserved flavoprotein oxidoreductases,
            function in plexin-mediated axonal repulsion
  JOURNAL   Cell 109 (7), 887-900 (2002)
   PUBMED   12110185
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC016027.15, DA531335.1, CR456364.1, AC016026.15, DR731344.1,
            AB020626.1 and BC035213.1.
            
            On Nov 23, 2018 this sequence version replaced NM_015241.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000441493.7/ ENSP00000416015.2
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC016027.15        85011-85012         c
            3-340               DA531335.1         1-338
            341-2619            CR456364.1         1-2279
            2620-2620           AC016026.15        116540-116540       c
            2621-2979           CR456364.1         2281-2639
            2980-3167           DR731344.1         52-239              c
            3168-3394           AC016026.15        76410-76636         c
            3395-4088           AB020626.1         1-694
            4089-5542           AC016026.15        62014-63467         c
            5543-6398           AB020626.1         2149-3004
            6399-7575           AC016026.15        34061-35237         c
            7576-9447           BC035213.1         1-1872
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.21"
     gene            1..9447
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /note="microtubule associated monooxygenase, calponin and
                     LIM domain containing 3"
                     /db_xref="GeneID:57553"
                     /db_xref="HGNC:HGNC:24694"
                     /db_xref="MIM:608882"
     exon            1..281
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            282..619
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             356..6364
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /EC_number="1.14.13.225"
                     /note="isoform 1 is encoded by transcript variant 1;
                     flavoprotein oxidoreductase MICAL3; protein MICAL-3;
                     molecule interacting with CasL protein 3;
                     protein-methionine sulfoxide oxidase MICAL3;
                     [F-actin]-methionine sulfoxide oxidase MICAL3;
                     [F-actin]-monooxygenase MICAL3"
                     /codon_start=1
                     /product="[F-actin]-monooxygenase MICAL3 isoform 1"
                     /protein_id="NP_056056.2"
                     /db_xref="CCDS:CCDS46659.1"
                     /db_xref="GeneID:57553"
                     /db_xref="HGNC:HGNC:24694"
                     /db_xref="MIM:608882"
                     /translation="MEERKHETMNPAHVLFDRFVQATTCKGTLKAFQELCDHLELKPK
                     DYRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKACTNTKCLIIGAGPCGLRTA
                     IDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIR
                     QLQLILLKVALILGIEIHVNVEFQGLIQPPEDQENERIGWRALVHPKTHPVSEYEFEV
                     IIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQ
                     ELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEA
                     LLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLL
                     VALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQ
                     TTPENVSKNFSQYSIDPVTRYPNINVNFLRPSQVRHLYDTGETKDIHLEMESLVNSRT
                     TPKLTRNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLID
                     FDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFK
                     DSLPSSDTLDLNAEEKAVLIASTRSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRR
                     KTSQSEEEEAPRGHRGERPTLVSTLTDRRMDVAVGNQNKVKYMATQLLAKFEENAPAQ
                     SIGIRRQGSMKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEYCATTL
                     RLSAYAYDIEDGKFYCKPHYCYRLSGYAQRKRPAVAPLSGKEAKGPLQDGATTDANGR
                     ANAVASSTERTPGSGVNGLEEPSIAKRLRGTPERIELENYRLSLRQAEALQEVPEETQ
                     AEHNLSSVLDTGAEEDVASSSSESEMEEEGEEEEEEPRLPPSDLGGVPWKEAVRIHAL
                     LKGKSEEELEASKSFGPGNEEEEEEEEEYEEEEEEDYDEEEEESSEAGNQRLQQVMHA
                     ADPLEIQADVHWTHIREREEEERMAPASESSASGAPLDENDLEEDVDSEPAEIEGEAA
                     EDGDPGDTGAELDDDQHWSDSPSDADRELRLPCPAEGEAELELRVSEDEEKLPASPKH
                     QERGPSQATSPIRSPQESALLFIPVHSPSTEGPQLPPVPAATQEKSPEERLFPEPLLP
                     KEKPKADAPSDLKAVHSPIRSQPVTLPEARTPVSPGSPQPQPPVAASTPPPSPLPICS
                     QPQPSTEATVPSPTQSPIRFQPAPAKTSTPLAPLPVQSQSDTKDRLGSPLAVDEALRR
                     SDLVEEFWMKSAEIRRSLGLTPVDRSKGPEPSFPTPAFRPVSLKSYSVEKSPQDEGLH
                     LLKPLSIPKRLGLPKPEGEPLSLPTPRSPSDRELRSAQEERRELSSSSGLGLHGSSSN
                     MKTLGSQSFNTSDSAMLTPPSSPPPPPPPGEEPATLRRKLREAEPNASVVPPPLPATW
                     MRPPREPAQPPREEVRKSFVESVEEIPFADDVEDTYDDKTEDSSLQEKFFTPPSCWPR
                     PEKPRHPPLAKENGRLPALEGTLQPQKRGLPLVSAEAKELAEERMRAREKSVKSQALR
                     DAMARQLSRMQQMELASGAPRPRKASSAPSQGKERRPDSPTRPTLRGSEEPTLKHEAT
                     SEEVLSPPSDSGGPDGSFTSSEGSSGKSKKRSSLFSPRRNKKEKKSKGEGRPPEKPSS
                     NLLEEAAAKPKSLWKSVFSGYKKDKKKKADDKSCPSTPSSGATVDSGKHRVLPVVRAE
                     LQLRRQLSFSEDSDLSSDDVLEKSSQKSRREPRTYTEEELNAKLTRRVQKAARRQAKQ
                     EELKRLHRAQIIQRQLQQVEERQRRLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKL
                     VQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELSEEKQILN
                     EMLEVVEQRDSLVALLEEQRLREREEDKDLEAAMLSKGFSLNWS"
     exon            620..827
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            828..944
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1046
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1202
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1303
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1561
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1677
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1804
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1901
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2049
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2246
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2359
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2422
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2423..2596
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2597..2783
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2784..2872
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2873..2960
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2961..3156
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3157..3410
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3411..3548
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3549..3662
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3663..3803
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3804..3886
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3887..5705
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            5706..5800
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            5801..5911
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            5912..6005
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            6006..6105
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            6106..6179
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            6180..9447
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9427..9432
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /note="hexamer: AATAAA"
     polyA_site      9447
                     /gene="MICAL3"
                     /gene_synonym="MICAL-3"
                     /note="major polyA site"
ORIGIN      
        1 agcagcgcgc gctcctgcag ccccggcgga gtcccggcgg ggagcgcccg cgtaggcagg
       61 cacccggggc agccctccgt cctcgccgcg cggcctgaga acccctgggc cctgggcgag
      121 ggggctgggc tgtgcagccc ccgaggctaa ctgggcggga tcgcagctgc aggggttgtg
      181 cgagcagcca cggcggcacc tgtgccctgc gggcacccac cctgcccctg ccgaggcagg
      241 cgcccaccgc cggcggtgat gcggctgtga agccctagtc gattaacgtg acagtgtcag
      301 gtggacccac agccatccca cctccccctc tggggagtgc tgagagtgag gcagcatgga
      361 ggagaggaag catgagacca tgaacccagc tcatgtcctc tttgaccggt ttgtccaggc
      421 caccacctgc aagggaaccc tcaaggcttt ccaggagctc tgtgaccacc tggaactaaa
      481 gccaaaggac taccgctcct tctatcacaa gctcaagtcc aagcttaact actggaaagc
      541 caaagccctc tgggcaaaat tggacaaacg gggcagtcac aaagactaca aaaagggaaa
      601 agcgtgcact aacaccaagt gtctcatcat tggggctggc ccctgtggtc tccgtacagc
      661 catcgactta tccttactgg gggccaaggt ggttgttatt gagaaacgag atgccttctc
      721 ccgcaacaac gtcttgcatc tctggccatt caccatacat gatctacgag gtctgggtgc
      781 caagaagttc tatggcaagt tctgtgctgg agccatcgac catatcagta tccgtcagct
      841 ccaactaata cttttgaaag tagccttgat cctaggcatt gaaatccacg tcaatgtgga
      901 attccaagga cttatacagc ctcctgagga ccaagagaat gaacggatag gctggcgggc
      961 actggtgcac cccaagactc atcctgtgtc agagtatgaa tttgaagtga tcatcggtgg
     1021 ggatggtcgg aggaacacct tggaagggtt tcgtcggaaa gaattccgtg gcaaactggc
     1081 catcgccatc acggcaaatt ttatcaaccg aaatacaaca gcagaagcta aagtggaaga
     1141 gatcagtggt gtggctttta tattcaacca aaaatttttc caggaactga gggaagccac
     1201 aggtattgac ttggagaaca tcgtttacta caaagatgac acacactatt tcgttatgac
     1261 agccaaaaag cagagtttgc tggacaaagg agtgatacta catgactacg ccgacacaga
     1321 gctcctgctt tcccgagaaa acgtggacca ggaggctctg ctcagctatg ccagggaggc
     1381 ggcagacttc tctacccagc agcagctgcc gtctctggat tttgccatca atcactatgg
     1441 gcagcccgat gtggccatgt ttgacttcac ttgtatgtat gcctccgaga acgccgcctt
     1501 ggtgcgggag cagaacggac accagttact agtggctctg gtcggggaca gcctcctaga
     1561 gcctttctgg ccaatgggaa caggaatagc ccggggcttt ctagctgcta tggactctgc
     1621 ctggatggtc cgaagttggt ctctaggaac gagccctttg gaagtgctgg cagagaggga
     1681 aagtatttac aggttgctgc ctcagaccac ccctgagaat gtgagtaaga acttcagcca
     1741 gtacagtatc gaccctgtca ctcggtatcc caatatcaac gtcaacttcc tccggccaag
     1801 ccaggtgcgc catttatatg atactggcga aacaaaagat attcacctgg aaatggagag
     1861 cctggtgaat tcccgaacca cccccaaatt gactcgcaat gagtctgtag ctcgttcaag
     1921 caaactgctg ggttggtgcc agaggcagac agatggctat gcaggggtaa acgtgacaga
     1981 tctcaccatg tcctggaaaa gtggcttggc cctttgtgca attatccata gataccgccc
     2041 tgacctgata gattttgatt ctttggatga gcaaaatgtg gagaagaata accaactggc
     2101 ctttgacatt gctgagaagg aattgggcat ttctcccatc atgacaggca aagaaatggc
     2161 ctccgtgggg gagcctgata agctgtccat ggtgatgtac ctgactcagt tctacgagat
     2221 gtttaaggac tccctcccct ctagcgacac cttggaccta aatgccgagg agaaagcagt
     2281 cctgatagcc agcaccagat cccctatctc cttcctaagc aaacttggcc agaccatctc
     2341 tcggaagcgt tctcccaagg ataaaaagga aaaggacttg gatggtgctg ggaagaggag
     2401 aaagaccagt caatcagagg aggaggaagc tcctcggggc cacagaggag aaagaccgac
     2461 cctggtgagc actctgacag acaggaggat ggacgttgcc gttgggaacc agaacaaagt
     2521 gaagtacatg gcgacccagc tgctggccaa atttgaagag aatgcgcccg cacagtccat
     2581 cggcatacgg agacagggct ccatgaagaa ggagttcccg cagaacctgg gaggcagcga
     2641 cacatgctac ttctgccaga agcgggtcta cgtgatggag aggctgagtg ccgagggcaa
     2701 gttcttccac cggagctgct tcaagtgcga gtactgcgcc accaccctgc gcctctcggc
     2761 ctacgcctac gacatcgagg atggtaaatt ctactgtaag ccacactact gctatcgact
     2821 ctctggctac gcacaaagga agagaccggc agtggctccc ctgtctggaa aggaggccaa
     2881 aggacccctg caggatggcg ccaccacaga tgcaaacgga cgggccaacg ccgtggccag
     2941 ctccactgag agaaccccag gttcaggcgt gaacggcctg gaggagccca gcatcgccaa
     3001 gcgactgagg ggcaccccag agcggatcga gctggagaac taccgcctgt ccctgaggca
     3061 ggctgaggca ctgcaggagg taccggagga gactcaggcc gagcacaacc tgagcagcgt
     3121 gctggacacg ggcgccgagg aggacgtcgc cagcagtagt tccgagtctg agatggagga
     3181 ggagggagag gaggaggagg aggagcctcg cctgccccca tctgacctgg gtggtgttcc
     3241 gtggaaggag gccgtgcgga tccatgccct tctgaaaggg aaaagtgaag aggagctaga
     3301 ggcctcaaag agctttgggc ctgggaatga agaggaggag gaggaggagg aagaatatga
     3361 agaggaggag gaggaggact atgacgagga ggaggaagag tccagtgaag ccgggaacca
     3421 gaggctccag caggtcatgc acgcggcgga tcctctggag atccaggctg acgtgcactg
     3481 gactcatatc cgtgagagag aggaggaaga gaggatggcg ccggcctctg agtcctctgc
     3541 ttccggagcc ccattggatg agaatgacct agaggaagat gtggactcag aaccagccga
     3601 gatagaaggg gaggcagcag aggatgggga cccaggggac actggtgctg agctggatga
     3661 tgatcagcac tggtctgaca gcccgtcgga tgctgacaga gagctgcgtt tgccgtgccc
     3721 agctgagggg gaagcagagc tggagctgag ggtgtcggaa gatgaggaga agctgcccgc
     3781 ctcaccgaag caccaagaga gaggtccctc ccaagccacc agccccatcc ggtctcccca
     3841 ggaatcagct cttctgttca ttccagtcca cagcccctca acagaggggc cccaactccc
     3901 acctgtccct gccgccaccc aggagaaatc acctgaggag cgccttttcc ctgagccttt
     3961 gctccccaaa gagaagccca aagctgatgc cccctcggat ctgaaagctg tgcactctcc
     4021 catccgatca cagccagtga ccctgccaga agctaggact cctgtctcac cagggagccc
     4081 gcagccccag ccacccgtgg cggcctccac gcccccaccc agcccactcc ccatctgctc
     4141 ccagccccag ccttccaccg aggccactgt cccatcccct acccagtccc ccatacgctt
     4201 ccagcctgcc ccggccaaaa catccacccc actggcccct ctccctgtcc aaagccaaag
     4261 tgacaccaag gacagactgg gcagccccct tgctgtggat gaggccctca gacggagcga
     4321 cctggtggag gagttctgga tgaagagtgc ggagatccgc cgcagcctcg ggctcacacc
     4381 tgtggaccgc agcaaggggc ccgagcccag cttccccacg cctgccttca ggccagtgtc
     4441 cctcaaatcc tattccgttg aaaagtcccc ccaggatgag ggactccacc ttctcaagcc
     4501 tctgtccatc cccaaaaggc tgggcctgcc aaagccggaa ggcgagccgt tgtccctgcc
     4561 aaccccccgg tccccgtccg acagagagct acgcagcgcc caggaggagc gcagggagct
     4621 gtccagcagc tctggcctgg gcctgcacgg gagctcctcc aacatgaaga cactgggcag
     4681 ccagagcttc aacacctcgg actccgccat gctcacgccc ccctccagcc cgcccccacc
     4741 gccacccccg ggcgaggagc ccgccacctt gcggaggaag ctcagggagg ccgagcccaa
     4801 tgcctcggtg gtcccgccgc ccttgcccgc cacctggatg cggccccccc gggagcctgc
     4861 tcagcccccc agagaggagg tgcggaagtc gtttgtggag agcgtggagg agattccctt
     4921 tgctgatgat gtggaggaca cctatgacga caagactgag gactcaagcc tgcaggagaa
     4981 attcttcacg cccccgtcct gctggccgcg ccccgagaag cctcgccacc cgcccctggc
     5041 caaggagaac gggaggctgc ctgctctgga ggggacgctg cagccacaga agagggggct
     5101 gcccttggtg tccgcggaag ccaaggagtt ggccgaggag cgcatgcgag ccagggagaa
     5161 gtccgtgaag agccaggcgc tgcgggacgc catggccagg cagctgagca ggatgcagca
     5221 gatggagctg gcctcaggcg cccccaggcc ccgcaaggcg tcctcagcac cctcccaggg
     5281 caaggagcgc cggcctgact cccccacacg ccccactctc aggggctccg aggagcccac
     5341 cctgaagcat gaagccacca gcgaggaggt cctctccccg ccgtcggact cagggggccc
     5401 agatggctct ttcacttcat ccgagggctc cagtgggaag agcaagaaga ggtcgtcact
     5461 cttctccccc cgcagaaaca agaaggagaa gaagtccaaa ggcgagggcc ggcccccgga
     5521 gaagcccagc tccaacctcc tagaagaagc cgccgccaaa cccaagtccc tgtggaagtc
     5581 cgtcttctcc gggtacaaga aggacaagaa gaagaaggcc gacgacaagt cctgccccag
     5641 caccccctcc agcggggcca cggtggactc tggaaagcac agggtgcttc ccgtcgtaag
     5701 ggcagagctg cagctccggc gccagctgag cttctccgag gactcagacc tctccagcga
     5761 cgatgtcctt gagaagtcct cacagaagtc ccggcgagag ccaagaacct acacggagga
     5821 ggaactgaat gccaagctga cccggcgtgt gcaaaaggca gctcggagac aggccaagca
     5881 ggaggagctt aagcggctgc atcgagccca gatcatccag cggcagctgc agcaggtgga
     5941 ggagaggcag cggcggctgg aggaaagggg cgtggctgtg gagaaggcgc tccggggcga
     6001 agcaggcatg ggcaagaagg acgaccccaa gctgatgcag gagtggttca agctagtgca
     6061 ggagaagaac gccatggtgc gctacgagtc ggagctgatg atctttgccc gggagctgga
     6121 gctggaagac cggcagagtc gactgcagca ggagctccgg gaacgcatgg cagtggaaga
     6181 tcaccttaag actgaggagg agctgtcaga agagaagcag attctcaatg agatgctgga
     6241 ggtggtggag cagagagact cactggtggc gctgctggag gagcagcggc tccgggagag
     6301 agaggaggac aaggacctgg aggctgccat gctgtccaag ggcttcagcc ttaactggtc
     6361 ctgagctccc acccaacgct ccattttctg ttggcatccg cctggccagg cagtggcatc
     6421 caaaccaccc ggagccgcga tctgaggagg cctggcacct ccttggagtt tacgctcaga
     6481 tgcccgtgtg ctgcttggaa agtggtcgag tcccgcgtgc agtggggagc cccaggtgac
     6541 agtggttatc tgagacggct ccacctcctg ggaggaggcc cacctggacc tcccactcag
     6601 aggagcagca cggcgtgtat ggcatgacgc aggggaccac cccgcgcgct ccctgaggat
     6661 gtgctggctg tgcccctttt ttccactggc acatttggta agagagggaa gctgctcccc
     6721 gtcagaacca cagtgcgccg tgcgaggggc actgtcttct tcatgctccc tggagcacca
     6781 ccaaagaaac gtaaacaata ccccacgaaa gcagggtcag gggtcagggt gcgatcgaga
     6841 cccaggatgg gggcgtccag tcatgcccac cccagcatca caggagacat ggaggtgcgg
     6901 gcaggctcct gaattattat gcaaattagg aggacgcagg aggggtctgc cctccagccg
     6961 aacaccacac actggaccct aagtggccaa atgcctgggc cgcttgctgg ctgtggcctg
     7021 aggcttgtgg gttgctgcat tttgcttgta gttcacaacc attttgacac tggaaaatgc
     7081 tgactttggg ggacaggatg aggccctaca ttctaagccc ccagttggca gacaggcatt
     7141 gtccctgttc cacatttatg tcgggacagg agatgacctt ttcctccgtg tttttcctgt
     7201 gtttgcacgt tgaaatgaag ctgacaacct ggcaagacgc tcagccgctt caaacccttt
     7261 ttgtcaatta acttattttt taatacttga aaagaagtaa cttcgtttgt gtatctttac
     7321 tagaggaact gatcacctgc gcccgggtgc gggagccaca gcggcatctg gtgcgtccta
     7381 cgcgacctgg tccggggctg cccggtgctc ctcacgtgca tctatttatt agcctttctc
     7441 ttcgtatcac tggcctggct ggcatcaggg agctgcccag aacccctgtg tgggtcctcc
     7501 tcacagcttt ctgtcccctc ctccacccgg tgcctgcctc accctggcgc tagaccatct
     7561 ggaccactca tgtgatgagg gtgcatttcc gttctgtttt gggccaggcc aacagcagag
     7621 ctgccactct caccctccca gtgagaattc cggctctgca gaactcgccc ttgtctcagt
     7681 ttgggggcca gggcatcacc ttcctccgca catatgttaa agaaggtttc aggatgggcc
     7741 ctcatccaca caggccaaga gggtgcaagg tgggaccctg gaatcatgtg gctggtgaga
     7801 attccctcct cccagcctaa gattcaccca gacggaaacg cgagtgctgc agtggatgct
     7861 gggatgcagg ctggtgcctc tcaagcagat cagcgactcc cgcccttcct tccgaaggcg
     7921 acgggcacct gccttgtgcc ggatcttcat ggggacataa agggcgagcc ccgaatcact
     7981 agctcctata gccaaactgt tcctttctca cggttccgca ccagcctggc tgtgtacagc
     8041 tcatcaagcc actgagctaa tcggggtggg gtgcttgtcc atcaaagcag tcagcacata
     8101 gcccagcgag gagccatccg gaccagaccc gcctgccagg ggcgctccag ccgcctgccg
     8161 cctccgtggg ctggggccag ctgggagcag aggcctggcc cctccaaggc gtcccgcagt
     8221 ggagcaggct gagtggctgt gctgaccttg ggctttccat gggaaccaca ctgtgcttca
     8281 acttgaacat tcatcccagc tgcaaaggag caaagaacct gtgtcatcct tgtctgtcca
     8341 gaagctgcca tctctctccc atgcacatcc gggagataac gccgctagtg gccgccacag
     8401 cgctgattct ccacctgttc tcacgtgagg caaaggtctc cttttctttt ctctttactt
     8461 aaaaaagggg agaggagggt ttctagattc catcttcaaa ccccagtcgt cccataaaat
     8521 tggacgtggg aaaagacctt cactgcctgc gtggcctttc ccagacctct cctcgaatgc
     8581 cacaaaaccg gtccagcccc ggcagagccg ccttcggccc ttgtagctcc tgctggcccc
     8641 acaacaggga aacagtttgc aaagtggctt ggaaggagtg tggcctaggg atctgctgta
     8701 agtggccaga cgtagcagga gagcccagtg tcaccttctg gtcctggtca gccttaacac
     8761 tgtggcatcc tccacagcac acacggcagc ctgcagtctg gaaggtggac ccgagccttt
     8821 gcagagaggc gccgcagagc cgcaggcctg cgccccagcc ttctgctccc gactgtgagg
     8881 acacccagtt ctagtagagc acttttttta aagctcccat tttgtaacca ctagtttgcg
     8941 gttgacttga gtactctggt gacttcctgc gtcaagcgtt ctcaagctgt gagaatgtgc
     9001 gcagctccag gcaggttttc tctcggagag ttaagtcttc ccttgaaggc agggaagcag
     9061 gatggataca catatatcac acgcataaaa caccaggtgc gggagcagcc cagactcaag
     9121 gctgactaaa ctggaggctg aataccgtgg aggtccacat gcagcttccc tggagggcag
     9181 gccggaggcg ctcccgcccc tgggcttgag gatgctgcac cccgtgggct tccaggcctg
     9241 cccagatgat gccttcaggc ctctgtccct ggcggccatc ctcaggccga ttttgaccag
     9301 caatgataga ctcttcttaa ccctttcaaa ataaattttt cagtgggaca gaaaggagag
     9361 ttaaaaaaca tttttttaaa ggtggtaaca tctgacccac aaagggaatg ggtctgtttt
     9421 atgcaaaata aaagtttttc aaataaa
//
