LOCUS       NM_001170687            3206 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens MIB E3 ubiquitin protein ligase 2 (MIB2), transcript
            variant 3, mRNA.
ACCESSION   NM_001170687
VERSION     NM_001170687.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Kong,Y., Li,J., Liang,X. and Zhou,X.
  TITLE     MIB2 promotes the progression of non-small cell lung cancer by
            regulating cell cycle control pathways
  JOURNAL   Genes Genomics 45 (9), 1143-1152 (2023)
   PUBMED   37436668
  REMARK    GeneRIF: MIB2 promotes the progression of non-small cell lung
            cancer by regulating cell cycle control pathways.
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Guo,Y., Chi,X., Qu,S., Sun,Y., Liu,J., Zhang,L., Cao,C., Wang,Y.
            and Zhang,W.
  TITLE     E3 ubiquitin-protein ligase 2 inhibits cell proliferation,
            migration, and invasion of non-small cell lung cancer through
            ubiquitination of Notch1
  JOURNAL   Acta Histochem 124 (1), 151818 (2022)
   PUBMED   34971948
  REMARK    GeneRIF: E3 ubiquitin-protein ligase 2 inhibits cell proliferation,
            migration, and invasion of non-small cell lung cancer through
            ubiquitination of Notch1.
REFERENCE   3  (bases 1 to 3206)
  AUTHORS   Nakabayashi,O., Takahashi,H., Moriwaki,K., Komazawa-Sakon,S.,
            Ohtake,F., Murai,S., Tsuchiya,Y., Koyahara,Y., Saeki,Y.,
            Yoshida,Y., Yamazaki,S., Tokunaga,F., Sawasaki,T. and Nakano,H.
  TITLE     MIND bomb 2 prevents RIPK1 kinase activity-dependent and
            -independent apoptosis through ubiquitylation of cFLIPL
  JOURNAL   Commun Biol 4 (1), 80 (2021)
   PUBMED   33469115
  REMARK    GeneRIF: MIND bomb 2 prevents RIPK1 kinase activity-dependent and
            -independent apoptosis through ubiquitylation of cFLIPL.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3206)
  AUTHORS   Uematsu,A., Kido,K., Takahashi,H., Takahashi,C., Yanagihara,Y.,
            Saeki,N., Yoshida,S., Maekawa,M., Honda,M., Kai,T., Shimizu,K.,
            Higashiyama,S., Imai,Y., Tokunaga,F. and Sawasaki,T.
  TITLE     The E3 ubiquitin ligase MIB2 enhances inflammation by degrading the
            deubiquitinating enzyme CYLD
  JOURNAL   J Biol Chem 294 (38), 14135-14148 (2019)
   PUBMED   31366726
  REMARK    GeneRIF: Results suggest that MIB2 enhances NF-kappaB signaling in
            inflammation by promoting the ubiquitin-dependent degradation of
            CYLD.
REFERENCE   5  (bases 1 to 3206)
  AUTHORS   Feltham,R., Jamal,K., Tenev,T., Liccardi,G., Jaco,I.,
            Domingues,C.M., Morris,O., John,S.W., Annibaldi,A., Widya,M.,
            Kearney,C.J., Clancy,D., Elliott,P.R., Glatter,T., Qiao,Q.,
            Thompson,A.J., Nesvizhskii,A., Schmidt,A., Komander,D., Wu,H.,
            Martin,S. and Meier,P.
  TITLE     Mind Bomb Regulates Cell Death during TNF Signaling by Suppressing
            RIPK1's Cytotoxic Potential
  JOURNAL   Cell Rep 23 (2), 470-484 (2018)
   PUBMED   29642005
  REMARK    GeneRIF: MIB2 represses the cytotoxic potential of RIPK1 by
            ubiquitylating lysine residues in the C-terminal portion of RIPK1.
            Disruption of MIB2-mediated ubiquitylation, either by mutation of
            MIB2's E3 activity or RIPK1's ubiquitin-acceptor lysines,
            sensitizes cells to RIPK1-mediated cell death.
REFERENCE   6  (bases 1 to 3206)
  AUTHORS   Takeuchi,T., Adachi,Y., Sonobe,H., Furihata,M. and Ohtsuki,Y.
  TITLE     A ubiquitin ligase, skeletrophin, is a negative regulator of
            melanoma invasion
  JOURNAL   Oncogene 25 (53), 7059-7069 (2006)
   PUBMED   16715130
  REMARK    GeneRIF: skeletrophin might be a novel suppressor factor for
            melanoma invasion
REFERENCE   7  (bases 1 to 3206)
  AUTHORS   Koo,B.K., Yoon,K.J., Yoo,K.W., Lim,H.S., Song,R., So,J.H., Kim,C.H.
            and Kong,Y.Y.
  TITLE     Mind bomb-2 is an E3 ligase for Notch ligand
  JOURNAL   J Biol Chem 280 (23), 22335-22342 (2005)
   PUBMED   15824097
  REMARK    GeneRIF: Mib2 has functional similarities to Mib1, but might have
            distinct roles in Notch signaling as an E3 ubiquitin ligase
REFERENCE   8  (bases 1 to 3206)
  AUTHORS   Takeuchi,T., Adachi,Y. and Ohtsuki,Y.
  TITLE     Skeletrophin, a novel ubiquitin ligase to the intracellular region
            of Jagged-2, is aberrantly expressed in multiple myeloma
  JOURNAL   Am J Pathol 166 (6), 1817-1826 (2005)
   PUBMED   15920166
  REMARK    GeneRIF: skeletrophin is a novel ubiquitin ligase that targets the
            intracellular region of Jagged-2 and is aberrantly overexpressed in
            multiple myeloma cells.
REFERENCE   9  (bases 1 to 3206)
  AUTHORS   Takeuchi,T., Adachi,Y. and Ohtsuki,Y.
  TITLE     Skeletrophin, a novel RING molecule controlled by the chromatin
            remodeling complex, is downregulated in malignant melanoma
  JOURNAL   DNA Cell Biol 24 (5), 339-344 (2005)
   PUBMED   15869411
  REMARK    GeneRIF: Skeletrophin, a novel RING molecule controlled by the
            chromatin remodeling complex, is down-regulated in melanoma.
REFERENCE   10 (bases 1 to 3206)
  AUTHORS   Takeuchi,T., Heng,H.H., Ye,C.J., Liang,S.B., Iwata,J., Sonobe,H.
            and Ohtsuki,Y.
  TITLE     Down-regulation of a novel actin-binding molecule, skeletrophin, in
            malignant melanoma
  JOURNAL   Am J Pathol 163 (4), 1395-1404 (2003)
   PUBMED   14507647
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL691432.54 and FO704657.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 20, 2020 this sequence version replaced NM_001170687.3.
            
            Summary: The protein encoded by this gene is an E3 ubiquitin
            protein ligase that mediates ubiquitination of proteins in the
            Notch signaling pathway. The encoded protein may be a suppressor of
            melanoma invasion. [provided by RefSeq, Mar 2017].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016490.1, AB076691.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000355826.10/
                                                ENSP00000348081.6
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AL691432.54        36330-36463
            135-241             AL691432.54        37338-37444
            242-510             AL691432.54        44261-44529
            511-682             AL691432.54        44604-44775
            683-789             AL691432.54        45625-45731
            790-984             AL691432.54        45821-46015
            985-1127            AL691432.54        46116-46258
            1128-1235           AL691432.54        46376-46483
            1236-1340           AL691432.54        47480-47584
            1341-1503           AL691432.54        47667-47829
            1504-1637           AL691432.54        47904-48037
            1638-1786           AL691432.54        48126-48274
            1787-1943           AL691432.54        48503-48659
            1944-2104           FO704657.2         41-201
            2105-2231           FO704657.2         295-421
            2232-2465           FO704657.2         511-744
            2466-2644           FO704657.2         1155-1333
            2645-2826           FO704657.2         1407-1588
            2827-2892           FO704657.2         1661-1726
            2893-3206           FO704657.2         2314-2627
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..3206
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /note="MIB E3 ubiquitin protein ligase 2"
                     /db_xref="GeneID:142678"
                     /db_xref="HGNC:HGNC:30577"
                     /db_xref="MIM:611141"
     exon            1..134
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            135..241
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            242..510
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     CDS             264..3131
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 3;
                     skeletrophin; zinc finger, ZZ type with ankyrin repeat
                     domain 1; E3 ubiquitin-protein ligase MIB2; novelzin; mind
                     bomb homolog 2; novel zinc finger protein; putative
                     NF-kappa-B-activating protein 002N; zinc finger ZZ type
                     with ankyrin repeat domain protein 1; mindbomb homolog 2;
                     RING-type E3 ubiquitin transferase MIB2; mindbomb E3
                     ubiquitin protein ligase 2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MIB2 isoform 1"
                     /protein_id="NP_001164158.3"
                     /db_xref="CCDS:CCDS41224.3"
                     /db_xref="GeneID:142678"
                     /db_xref="HGNC:HGNC:30577"
                     /db_xref="MIM:611141"
                     /translation="MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGS
                     PSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGM
                     RWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQG
                     AKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGK
                     VDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQ
                     EGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVV
                     RVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCL
                     VAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALD
                     LLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALG
                     NQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDT
                     PLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARAR
                     QLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVG
                     LVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGL
                     PGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQA
                     GGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEEC
                     ARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPIC
                     IDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV"
     exon            511..682
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            683..789
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            790..984
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1127
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1235
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1340
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1503
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1637
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1786
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            1787..1943
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2104
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2231
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2465
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            2466..2644
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            2645..2826
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            2827..2892
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     exon            2893..3206
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3185..3190
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /note="hexamer: TATAAA"
     polyA_site      3206
                     /gene="MIB2"
                     /gene_synonym="ZZANK1; ZZZ5"
                     /note="major polyA site"
ORIGIN      
        1 gcccttcggg tcccacagtt tccagccgcc gctctcctca gtgcccggtg gcccaggagg
       61 gcctgggagc ccgaagccgt ccccgagtcg ctcctaggtc actggcgcga tgcgggccgt
      121 cctctcggct gatgggttgg aagcccagcg aggctagagg ccagtcccaa agtttccagg
      181 catcagggct gcagcccagg agcctcaagg cggcccggcg ggcgactgga cggccggaca
      241 ggtcccgagc agccccgccc aacatggacc cagaccccca ggcgggcgtg caggtgggca
      301 tgcgggtggt gcgcggcgtg gactggaagt ggggccagca ggacggcggc gagggcggcg
      361 tgggcacggt ggtggagctt ggccgccacg gcagcccctc gacacccgac cgcacagtgg
      421 tcgtgcagtg ggaccagggc acgcgcacca actaccgcgc cggctaccag ggcgcgcacg
      481 acctgctgct gtacgacaac gcccagatcg gcgtccggca ccccaacatc atctgtgact
      541 gctgcaagaa gcacgggctg cgggggatgc gctggaagtg ccgtgtgtgc ctggactacg
      601 acctctgcac gcagtgctac atgcacaaca agcatgagct cgcccacgcc ttcgaccgct
      661 acgagaccgc tcactcgcgc cctgtcacac tgagtccccg ccagggcctc ccgaggatcc
      721 cactaagggg catcttccag ggagcgaagg tggtgcgagg ccccgactgg gagtggggct
      781 cacaggatgg aggggaaggg aaaccgggcc gtgtggtgga catccgtggc tgggatgtgg
      841 agacaggccg gagtgtggcc agcgtgacgt gggctgatgg taccaccaat gtgtaccgtg
      901 tgggccacaa gggcaaggtg gacctcaagt gtgtgggcga ggcagcgggc ggcttctact
      961 acaaggacca cctcccaagg ctcggcaagc cggcggagct gcagcgcagg gtgagtgctg
     1021 acagccagcc cttccagcac ggggacaagg tcaagtgtct gctggacact gatgtcctgc
     1081 gggagatgca ggaaggccac ggcggctgga accccaggat ggcggagttt atcggacaga
     1141 cgggcaccgt gcatcgtatc acggaccgcg gggacgtgcg cgtgcagttc aaccacgaga
     1201 cgcgctggac cttccacccc ggggcgctca ccaagcacca ctccttctgg gtgggcgacg
     1261 tggtccgggt catcggcgac cttgacacag tgaagcggct gcaggctggg catggcgagt
     1321 ggacggacga catggcccct gccctgggcc gcgtcgggaa ggtggtgaaa gtgtttggag
     1381 acgggaacct gcgtgtagca gtcgctggtc agcggtggac cttcagcccc tcctgcctgg
     1441 tggcctaccg gcccgaggag gatgccaacc tggacgtggc cgagcgcgcc cgggagaaca
     1501 aaagctcact gagcgtggcc ctggacaagc ttcgggccca gaagagtgac ccagagcacc
     1561 cgggaaggct ggtggtggag gtggcgctgg gtaacgcagc ccgggctctg gacctgctgc
     1621 ggaggcgccc agagcaggtg gacaccaaga accaaggcag gaccgctctg caagtggctg
     1681 cctacctggg ccaggtggag ttgatacggc tgctgctaca agccagggcg ggcgtggacc
     1741 tgccggacga cgagggcaac acggcactgc actacgcggc cctggggaac cagcccgagg
     1801 ccaccagggt gctcctgagt gctgggtgcc gggcggacgc catcaacagc acccagagca
     1861 cagcactgca cgtggccgtg cagaggggct tcctggaggt ggtgcgggcc ctgtgtgagc
     1921 gcggctgtga cgtcaacctg cccgacgccc actcggacac gcccctgcac tccgccatct
     1981 cggcgggcac tggagccagc ggcattgtcg aggtcctcac ggaggtgcca aacatcgatg
     2041 ttaccgccac caacagccag ggtttcaccc tgctgcacca tgcctccctc aagggtcacg
     2101 cgctagctgt gagaaagatt ctggctcggg cgcggcagct ggtggacgcc aagaaggagg
     2161 acggcttcac ggcgctgcat ctggctgccc tcaacaacca ccgcgaggtg gcccagatcc
     2221 tcatccggga gggccgctgt gacgtgaacg tgcgcaaccg gaagctgcag tccccgctgc
     2281 atctcgccgt gcaacaggcc cacgtggggc tggtgccgct actggtggac gctgggtgca
     2341 gtgtcaacgc cgaggacgag gagggggaca cagccctgca cgtggcgctg cagcgtcatc
     2401 agctgctgcc cctggtggct gatggggccg ggggggaccc agggcccttg cagctgctgt
     2461 ccaggctaca ggcctcgggc ctccccggca gcgcggagct gacggtgggc gcggcggtcg
     2521 cctgcttcct ggcgctggag ggcgccgacg tgagctacac caaccaccgc ggtcggagcc
     2581 cgctggacct ggccgccgag ggtcgcgtgc tcaaggccct tcagggctgc gcccagcgct
     2641 tccgggagcg gcaggcgggc gggggcgcgg ccccgggccc caggcaaacg ctcgggaccc
     2701 ccaacaccgt gacgaacctg cacgtgggcg ccgcgccggg gcccgaggcc gctgagtgcc
     2761 tggtgtgctc cgagctggcg ctgctggtgc tgttctcgcc gtgccagcac cgcaccgtgt
     2821 gtgaggagtg cgcgcgcagg atgaagaagt gcatcaggtg ccaggtggtc gtcagcaaga
     2881 aactgcgccc agacggctct gaggtggcga gcgccgcccc cgcccccggc ccgccgcgcc
     2941 agctggtgga ggagctgcag agccgctacc ggcagatgga ggaacgcatc acctgcccca
     3001 tctgcatcga cagccacatc cgcctcgtgt tccagtgcgg ccacggcgca tgcgccccct
     3061 gcggctccgc gctcagcgcc tgccccatct gccgccagcc catccgcgac cgcatccaga
     3121 tcttcgtgtg agccgcgccg tccgccgcgc ccgagctgcc ttcgcgtgcc cccgccctgt
     3181 gttttataaa aagaaagatt ctcgga
//
