LOCUS       NM_020774              10100 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens MIB E3 ubiquitin protein ligase 1 (MIB1), mRNA.
ACCESSION   NM_020774
VERSION     NM_020774.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Cao,R., Gozlan,O., Airich,A., Tveriakhina,L., Zhou,H., Jiang,H.,
            Cole,P.A., Aster,J.C., Klein,T., Sprinzak,D. and Blacklow,S.C.
  TITLE     Structural requirements for activity of Mind bomb1 in Notch
            signaling
  JOURNAL   Structure 32 (10), 1667-1676 (2024)
   PUBMED   39121852
  REMARK    GeneRIF: Structural requirements for activity of Mind bomb1 in
            Notch signaling.
REFERENCE   2  (bases 1 to 10100)
  AUTHORS   Xu,P., Liu,K., Huang,S., Lv,J., Yan,Z., Ge,H., Cheng,Q., Chen,Z.,
            Ji,P., Qian,Y., Li,B., Xu,H., Yang,L., Xu,Z. and Zhang,D.
  TITLE     N6-methyladenosine-modified MIB1 promotes stemness properties and
            peritoneal metastasis of gastric cancer cells by ubiquitinating
            DDX3X
  JOURNAL   Gastric Cancer 27 (2), 275-291 (2024)
   PUBMED   38252226
  REMARK    GeneRIF: N[6]-methyladenosine-modified MIB1 promotes stemness
            properties and peritoneal metastasis of gastric cancer cells by
            ubiquitinating DDX3X.
REFERENCE   3  (bases 1 to 10100)
  AUTHORS   Tessler,I., Albuisson,J., Pineiro-Sabaris,R., Verstraeten,A.,
            Kamber Kaya,H.E., Siguero-Alvarez,M., Goudot,G., MacGrogan,D.,
            Luyckx,I., Shpitzen,S., Levin,G., Kelman,G., Reshef,N., Mananet,H.,
            Holdcraft,J., Muehlschlegel,J.D., Peloso,G.M., Oppenheim,O.,
            Cheng,C., Mazzella,J.M., Andelfinger,G., Mital,S., Eriksson,P.,
            Billon,C., Heydarpour,M., Dietz,H.C., Jeunemaitre,X.,
            Leitersdorf,E., Sprinzak,D., Blacklow,S.C., Body,S.C., Carmi,S.,
            Loeys,B., de la Pompa,J.L., Gilon,D., Messas,E. and Durst,R.
  TITLE     Novel Association of the NOTCH Pathway Regulator MIB1 Gene With the
            Development of Bicuspid Aortic Valve
  JOURNAL   JAMA Cardiol 8 (8), 721-731 (2023)
   PUBMED   37405741
  REMARK    GeneRIF: Novel Association of the NOTCH Pathway Regulator MIB1 Gene
            With the Development of Bicuspid Aortic Valve.
REFERENCE   4  (bases 1 to 10100)
  AUTHORS   Kanoh,T., Lu,J., Mizoguchi,T. and Itoh,M.
  TITLE     The E3 ubiquitin ligase MIB1 suppresses breast cancer cell
            migration through regulating CTNND1 protein level
  JOURNAL   Biochem Biophys Res Commun 667, 73-80 (2023)
   PUBMED   37209565
  REMARK    GeneRIF: The E3 ubiquitin ligase MIB1 suppresses breast cancer cell
            migration through regulating CTNND1 protein level.
REFERENCE   5  (bases 1 to 10100)
  AUTHORS   Zhang,L., Nephew,K.P. and Gallagher,P.J.
  TITLE     Regulation of death-associated protein kinase. Stabilization by
            HSP90 heterocomplexes
  JOURNAL   J Biol Chem 282 (16), 11795-11804 (2007)
   PUBMED   17324930
  REMARK    GeneRIF: DAPK is found in two distinct immune complexes, one
            containing HSP90 and CHIP and a second complex containing only
            DIP1/Mib; strict modulation of DAPK activities by HSP90
            heterocomplexes is critical for regulation of apoptosis and
            cellular homeostasis
REFERENCE   6  (bases 1 to 10100)
  AUTHORS   Song,R., Koo,B.K., Yoon,K.J., Yoon,M.J., Yoo,K.W., Kim,H.T.,
            Oh,H.J., Kim,Y.Y., Han,J.K., Kim,C.H. and Kong,Y.Y.
  TITLE     Neuralized-2 regulates a Notch ligand in cooperation with Mind
            bomb-1
  JOURNAL   J Biol Chem 281 (47), 36391-36400 (2006)
   PUBMED   17003037
  REMARK    GeneRIF: Neuralized-2 regulates a Notch ligand in cooperation with
            Mind bomb-1
REFERENCE   7  (bases 1 to 10100)
  AUTHORS   Yoo,K.W., Kim,E.H., Jung,S.H., Rhee,M., Koo,B.K., Yoon,K.J.,
            Kong,Y.Y. and Kim,C.H.
  TITLE     Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic
            and endothelial precursor cells in zebrafish
  JOURNAL   FEBS Lett 580 (18), 4409-4416 (2006)
   PUBMED   16857196
REFERENCE   8  (bases 1 to 10100)
  AUTHORS   Henshall,D.C., Schindler,C.K., So,N.K., Lan,J.Q., Meller,R. and
            Simon,R.P.
  TITLE     Death-associated protein kinase expression in human temporal lobe
            epilepsy
  JOURNAL   Ann Neurol 55 (4), 485-494 (2004)
   PUBMED   15048887
REFERENCE   9  (bases 1 to 10100)
  AUTHORS   Itoh,M., Kim,C.H., Palardy,G., Oda,T., Jiang,Y.J., Maust,D.,
            Yeo,S.Y., Lorick,K., Wright,G.J., Ariza-McNaughton,L.,
            Weissman,A.M., Lewis,J., Chandrasekharappa,S.C. and Chitnis,A.B.
  TITLE     Mind bomb is a ubiquitin ligase that is essential for efficient
            activation of Notch signaling by Delta
  JOURNAL   Dev Cell 4 (1), 67-82 (2003)
   PUBMED   12530964
REFERENCE   10 (bases 1 to 10100)
  AUTHORS   Jin,Y., Blue,E.K., Dixon,S., Shao,Z. and Gallagher,P.J.
  TITLE     A death-associated protein kinase (DAPK)-interacting protein,
            DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis
            factor-induced apoptosis and regulates the cellular levels of DAPK
  JOURNAL   J Biol Chem 277 (49), 46980-46986 (2002)
   PUBMED   12351649
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091038.9, AY149908.1 and
            AC103987.5.
            
            On Mar 31, 2020 this sequence version replaced NM_020774.3.
            
            Summary: This gene encodes a protein containing multiple ankyrin
            repeats and RING finger domains that functions as an E3 ubiquitin
            ligase. The encoded protein positively regulates Notch signaling by
            ubiquitinating the Notch receptors, thereby facilitating their
            endocytosis. This protein may also promote the ubiquitination and
            degradation of death-associated protein kinase 1 (DAPK1). [provided
            by RefSeq, Jun 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY147849.1, BC110582.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261537.7/ ENSP00000261537.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-792               AC091038.9         75634-76425         c
            793-3813            AY149908.1         1-3021
            3814-10100          AC103987.5         91940-98226
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q11.2"
     gene            1..10100
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /note="MIB E3 ubiquitin protein ligase 1"
                     /db_xref="GeneID:57534"
                     /db_xref="HGNC:HGNC:21086"
                     /db_xref="MIM:608677"
     exon            1..1021
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     CDS             793..3813
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /EC_number="2.3.2.27"
                     /note="ubiquitin ligase mind bomb; DAPK-interacting
                     protein 1; zinc finger ZZ type with ankyrin repeat domain
                     protein 2; RING-type E3 ubiquitin transferase MIB1;
                     mindbomb E3 ubiquitin protein ligase 1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MIB1"
                     /protein_id="NP_065825.1"
                     /db_xref="CCDS:CCDS11871.1"
                     /db_xref="GeneID:57534"
                     /db_xref="HGNC:HGNC:21086"
                     /db_xref="MIM:608677"
                     /translation="MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFES
                     PEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAE
                     CTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRG
                     VDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCV
                     QDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMF
                     ETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQ
                     VGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTY
                     NPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDL
                     LKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGD
                     EGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTP
                     LHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWI
                     VDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIV
                     RLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIM
                     GLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPNLCKALAKCHKEKVS
                     GQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQ
                     VQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCANLMKKCVQCRAVVERRVPFIMCC
                     GGKSSEDATDDISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKN
                     MIFLCGHGTCQLCGDRMSECPICRKAIERRILLY"
     exon            1022..1193
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1323
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1428
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1495
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1700
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1884
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2029
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2163
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2271
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2469
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            2470..2621
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2754
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2841
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            2842..3003
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            3004..3185
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            3186..3378
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            3379..3457
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            3458..3571
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            3572..3672
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     exon            3673..10100
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7945..7950
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /note="hexamer: ATTAAA"
     polyA_site      7966
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /note="major polyA site"
     regulatory      9294..9299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /note="hexamer: AATAAA"
     polyA_site      9317
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /note="major polyA site"
     regulatory      10081..10086
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /note="hexamer: AATAAA"
     polyA_site      10100
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
ORIGIN      
        1 cttggaccct ggagagacgc ttagggatca gttttctcct cctttcttcc cgcgatcgcg
       61 cggctctctg gaagccttcc cactctatta ttgccgagga tccccctcct agacactctg
      121 agaaggtgcc gctccggcct tgggtacggc ggtacccgga tgtggagtcg ctctcgcccg
      181 gctgggactc tgtggcgggg cggagcgcgg cggctggtgc gggggcagag agaagggggc
      241 ctgagggccc gggcgctcgc cggacgccgg gaggtaccac cgctgcccac gggggaggag
      301 gcggcgccgg cgcttgggtg cccgcccccg agcgaggggc cggggtggca gaaaggccgg
      361 cagttaagct tgctccccgc cgcccccctc gggctagcct cccccggccc cctgcgctcc
      421 tcgcgctgcc ggccggggga gagcgtggtt tccttgcggc cgtcgctgta gcctggccgg
      481 ggagcccgga ggaagcggga gagtccccgc ccacggcccc ctcccctctg cccgctctcc
      541 gccgccgcct ccgagcagcc gcgggccgcc ctccactccg agcggggggc gcggcggcga
      601 cagctgggca gcggctttgg gctccgcggg gaccgcgccg ccgcccccgt gagttattct
      661 cacgtccccc ggggctcgct gccgcccccg ccgacgccta gagtccggcc cgggcccaac
      721 tccctcacgg gccccccggc ggcagcggcg gcggcggcgg cggcagcggc ggagcccacc
      781 gcccgggccc cgatgagtaa ctcccggaat aaccgggtga tggtggaagg ggttggcgct
      841 cgggtagtgc gcggcccgga ctggaagtgg gggaagcagg acggcggcga gggccatgtg
      901 ggcaccgtcc ggagcttcga gagccccgag gaggtggtgg tagtgtggga caacggcaca
      961 gctgccaact accgctgctc cggggcttac gacctccgca tcctggacag cgcgcccacc
     1021 ggcatcaagc atgatggaac catgtgtgat acctgccgcc agcaaccaat cattggcatt
     1081 cgatggaagt gtgcagagtg tacaaattat gatttgtgca cagtgtgtta tcatggagat
     1141 aaacatcatt taagacatcg cttttaccga attactacac cgggaagtga gagggttctg
     1201 ttagagtctc gtaggaaatc taagaagatt acagccagag gaatctttgc aggtgccaga
     1261 gtggtgcgag gagtggactg gcagtgggaa gatcaagatg gaggaaatgg acgtagggga
     1321 aaggtaacag aaatccagga ctggagtgca tcaagcccac atagcgcagc atatgtcctc
     1381 tgggataatg gtgctaagaa cctttacaga gttggctttg agggcatgtc tgatctgaaa
     1441 tgtgtccagg atgccaaggg aggttctttc tacagagatc actgccctgt gctaggtgag
     1501 cagaatggca acaggaatcc tggtggattg cagattggtg acctggtaaa tatagatctc
     1561 gacctcgaaa ttgtacagtc tttgcagcat ggtcatggag gatggactga tggaatgttt
     1621 gagactttaa ctacaactgg aactgtttgt ggcattgatg aagatcatga cattgtagta
     1681 cagtatccaa gtggcaatag gtggaccttc aatcctgctg ttctcactaa agcgaacatt
     1741 gtccgaagtg gagatgctgc tcagggtgca gaaggaggca cctcgcagtt tcaagtgggt
     1801 gatcttgtac aagtttgtta tgacctggaa cgaattaaac ttctacaaag aggacatgga
     1861 gaatgggctg aagcgatgct tccaacttta ggtaaagttg gccgagtaca acagatttat
     1921 tcagacagtg atttaaaggt ggaagtttgt ggaacatctt ggacatacaa tccagcagca
     1981 gtttccaagg tggcatctgc aggatcagcc attagcaatg catctggtga aagactctca
     2041 caactcctga agaaattatt tgaaacccaa gaatctggtg acctcaatga agaattagtt
     2101 aaggctgctg ccaatggaga tgttgctaaa gtggaagatt tgcttaaaag accagatgtg
     2161 gatgtaaatg ggcaatgtgc tggccacaca gctatgcaag ctgctagtca gaatggacat
     2221 gttgacattt tgaagttact tttgaagcaa aacgtggatg tcgaagcaga ggataaagat
     2281 ggtgatagag cagttcacca tgcagctttt ggagatgaag gcgctgttat agaagtacta
     2341 catcgaggta gtgctgattt gaatgctcga aacaagcgcc gacagacacc acttcatatt
     2401 gctgtcaata aaggtcatct tcaagttgtg aagactttat tggactttgg ctgtcatccc
     2461 agtctccagg attctgaagg tgatacccct cttcatgatg caataagtaa gaaacgtgat
     2521 gatatcctag cagttctttt ggaagctgga gcagatgtta ccatcacaaa caataatgga
     2581 tttaatgctc tgcatcatgc tgcactaagg ggaaatccca gtgcaatgcg tgttttacta
     2641 tctaaattac caagaccatg gattgtggat gagaagaaag atgatggtta tactgcctta
     2701 catctggctg cccttaataa tcacgtagaa gtggctgaac tgttggtaca tcagggtaat
     2761 gcaaacctgg atatccagaa tgtgaaccaa caaactgccc tacaccttgc tgttgaacga
     2821 cagcataccc agattgttag gcttttggtc cgtgcaggtg ccaagcttga tattcaggat
     2881 aaggatgggg atactccttt gcatgaagct ctaaggcatc acactttgtc tcagctacgt
     2941 cagctccaag atatgcaaga tgtggggaag gtggatgctg cctgggagcc atccaaaaac
     3001 acgttaataa tgggacttgg tacccagggg gcagagaaga agagtgcagc atctattgcc
     3061 tgtttcttgg cagccaatgg tgctgacctg agcattcgaa ataagaaggg tcaatcgcca
     3121 cttgatctct gtcctgatcc gaatctctgc aaagcactgg caaagtgtca taaggaaaaa
     3181 gtcagtggtc aagtgggttc tcggagtcct tctatgatta gtaatgattc tgaaacctta
     3241 gaagagtgta tggtgtgctc agatatgaag agagatactc tttttggtcc atgtggacat
     3301 attgctacct gttctttatg ttctccacgt gtcaagaaat gcctcatctg taaagaacag
     3361 gttcaatcca ggacaaagat tgaagaatgt gtggtatgct ctgacaagaa agcagctgtt
     3421 ctttttcaac cctgtggcca catgtgtgct tgtgagaact gtgctaacct gatgaaaaag
     3481 tgtgtgcagt gtcgagcagt agttgaacga agagtgcctt tcattatgtg ctgtggaggg
     3541 aaaagttcag aagatgccac tgatgatatc tcaagtggga atattccagt attacaaaag
     3601 gacaaggata ataccaatgt caatgcagat gtgcaaaagt tgcagcaaca gttacaagac
     3661 attaaagagc agacaatgtg ccctgtgtgt ctagatcgtc tgaagaatat gattttcctt
     3721 tgtggtcacg gaacctgtca actctgtgga gaccgcatga gtgaatgtcc tatctgtcgc
     3781 aaggctattg aacgaaggat tcttttgtat taactaagac acatggtgta ttttgttagc
     3841 taatgtatct agtcatgaga tcttaatagg cttttgatct agttggaagt tctgatgagt
     3901 taatttctaa tatcatagtt tctttactag agtataattg ggctgtaaat gtaccagaac
     3961 aaaaaaccct acaaaatggt gttggaaatt gtgttttttg tttttgtttt aaatttgaaa
     4021 catcaaattc atgtaactca taggataatt tacctttggc ttctaagagg aaagtccttt
     4081 aaggatatcc ttttttaaaa aattgcattt ttctcttata atttgtaaat ttgttggatc
     4141 tcaaaagaca taattctttg tgatcagtta tccttcattt catcgtggtt ttacacagtg
     4201 agttgataac aggttctctg agaagtcatg catcaaataa aagaggcagg tcaaacaatt
     4261 atgtcacatg gtaaattata aaatgacagt acaagttcca gatagttaag ggaataccga
     4321 agggatgatt ctttttttaa gataacagga agttacccac atgtttgttt ctgaattctt
     4381 agagtaaatg gaagcataga atgagggaat aatgactttg catttctctt gttttctaga
     4441 ttcaaaagga acattgttta acttgaatca gattaccagt ttcaaggtga ctgatagaca
     4501 agaaaaggaa aaataagcaa taatagtggg caactgaaga gaaaaaaaaa acgagtatct
     4561 attaactggc cactaacagt tgcctttctt acattaattt atacactatt ttgttcagcc
     4621 agtgttttta aaaaaaatct atgaaaagtg tacttccggt tttctgtgat tacttatctg
     4681 ggcttgatct gaccagtgaa atgacattgc cctatttgga cctctgaggt tctatttagc
     4741 tttgcagatg tacatagtat cccagtgatc tgcaaaatta atgccttttc caagaaaaaa
     4801 tcttttcttc tctgtatcag ttaattctga cagtgttagt gattctgtct tcattatagg
     4861 ccttatttcc attatctctt tctttatagt attttttgtt ataaagaaaa cagtctttct
     4921 gtgtatacct acggatgagg gtattattta aactgccaac aatatccaag acatggtcaa
     4981 taacctaatt ataaatactt tagaaagagt gaccaggaca tgtatagaaa tgtctgctta
     5041 cctgtagact ttaaaaacaa acaaaatttt tggagcattt aatcattttt tttctccttt
     5101 tatctccttt gtaatcttat tgtctcctga gtaaatatac acataaatgt ttggggattc
     5161 attgctgcta gattatatca ggtgtttaca tagtgtctac tatatgctgt tgataagctt
     5221 tttcctaaaa atagttatcc tcttttgtag tttttttccc tcctttaaat ctggctagct
     5281 tttcttactt cagtttttat agaacaccaa ctaaatttga aagtatgaag tctctagccc
     5341 tcttacactg tttctggctg tttaatcctt gctgcctttg caagtcctgg tgaaagcaat
     5401 ggggagcttt gctggctggt tttgtagtct caacaattcc atgtctgtgt ttagctgagt
     5461 aactgctatc atttagaaca gtaaaatttt tatagatttc aaatttgaaa attcatgtac
     5521 actcagttat ctaataaggg gccaccgacg ctgtttttaa aactattttg aagttataaa
     5581 aaggtagaac atctgactga tgatgttcac ataaagagac agacagacta tcatgttgca
     5641 gacatgaaaa cagcatgtca ggtttttcct ttccttccat gccatgggct acccatctct
     5701 tcccactaga tcacctggtg aggagggagc acctggctga ctcatgcaga gtttggccaa
     5761 tcagagaatt gtgtggtctc cagctctact ttccttaaac aaatcctttc tgttttctga
     5821 gctgcagtgc taaaacagat ccctaaaagg taatagcatc ttccagacta gtttaattat
     5881 ttcctttata agaatatgac ataaattttt attatagaaa taatgtcatt ataattttag
     5941 tgctagtact tgtgggtttt ctgtatttgt atcaccttga aatttgttcc atgaatagaa
     6001 aatacttctt tattcatgtt ttcaatcact tagaattatt ttaaatttat tttgggtttg
     6061 tgagagataa taagattaag aacttcatca tttcagtgac tttcctccaa ctctctgaat
     6121 ctgctacctc ttattatatg tcccttagca gtacttttcc agttaggttc gttaccctgc
     6181 tcattagcta aacctcttta tcttgtgcct caaaagagct tttacttttc ttagaaacat
     6241 ttataaaaaa gaaaaaaact gacacgcatt cattcaaaaa agtatttatt gggctttagg
     6301 gattagatac acaacagtgg aaaaatggca tcttagcaca ctcagaattt attgtgcagc
     6361 agttgtgtat tttaaggaat ctgtagttaa aaggaagaga tccaaaggtc ctgataacat
     6421 ttcctaacgt ttcagaagag ttgtcattga gtagtgtttg gatttcctct ctgtatccct
     6481 caccctacca ttttctcctt tattcttcta agattgttag cgttgttact caaaacatac
     6541 acatacaaca taccagatgg tgctgttcta atggtatttg ttcacgtaat ttaacgttct
     6601 tactgcttat actatataca tatttaattt tgttgggagc aagaaggttt tcaagaaaag
     6661 ataattttgt attagtgtag gaatatatta gacaaatgtt tagttggtca aattatgcat
     6721 aagatatttg taactttaaa cattgcaaat tgaccagcta gctatggaag cataaaatta
     6781 attgataatt aatgttggaa atacatttct aaaatttata tttttatggt gttagcctgt
     6841 aagagcattt tagcatattg aaatgcatgc tgcttaagat gtgtctggtt caaatatcaa
     6901 aacttgctgg gtcattttta catagtagaa acatactgtc tatgtgttgc ccaattgctg
     6961 tttgtccact ctgaattctg gacccttttt ggactaagaa ttagttaaat tcatgaacta
     7021 agagaattag agatggggta gagtaggatc aggacagtgg ggttacaaca gataggaaag
     7081 gaaagtcagc atttgagtgt agagaaaaaa agaggatcat acagatgtca tcaaattata
     7141 ggctttaata caggttgctt tttttaatcc aaaaggaaaa gcacttatcc tatcagaatg
     7201 tctaccatgt tttatagtaa taatttgtgt aaaaaattga atttttgaat agttttttgt
     7261 attttttgtg atgaaaaact cataacataa aatttaccat attaactgtt tctaaagaat
     7321 acagttcagc catgtgaagt acatttacac tgttgtgaag cagatctcca gaactttttc
     7381 atcttgcaaa actgaaactc catacccatt aaacaactcc cctttttgtt ttctcctcag
     7441 tggaatagaa ttttgactcc atataaatca agaaacacct aaattcttta gttgtttgtg
     7501 tttgcaagat ctaaggtcat ggtaaacatt aagttcttaa aatttttggg agggacagtg
     7561 cacctctcct ctgaattgtt agcaattaaa ttgagtaggt tttaaatgtc tattcattgg
     7621 aagggtttgt tatttcattt tgagccagag gggagaggca cattttaaat atcagaatta
     7681 gattagcttt gagtttgtac aattgggaac ataatagatt ttcataaatt atgtgtgcct
     7741 tgttggaagt gtcaactgtc tttatgtctg cttgtaaaag tttcaaaata tgttttccct
     7801 caaaaaggca acgttacttc atttgcttga atattatgat aggaatgctt actgatatta
     7861 cttgatagtc atatatagcc taggaaattt aacatatata taactatagc agtattaata
     7921 atgatagttg tacttcttta aaacattaaa tttgaggaaa ctttaatgct gtctcgtgta
     7981 cattgcttta ctacagtgag ggggaatatc ctttagattg agcctcaatt tactggttag
     8041 tagtatgtga aactctggta taaaaacgta aactagacag tagagccgat gaattaaaat
     8101 tgtaaattgc tacattggca ttttctacct ccttttctgt cagagtatta ctttttccag
     8161 catttattct tatttgtgag taaagaggaa atgggaacct gaggttaaaa ttgacatttt
     8221 tgtttcattg agaatttaag cagtaggtac aggagaagtg acttgtcaca ttaatttggt
     8281 gcctaaatct gtaactacaa gttgtgatcg acatgtacaa aatgtctaag aaaggtcata
     8341 tgctgaatat tttacttttc ctgtatagtc tgcatgattt gtttcataaa cccagcttat
     8401 ttcctccaaa aagcaaaatg gtcctgtaat ttttaaagta aaataaacgt gccattttgt
     8461 ctgcaatcta taatttcagg aagttattga aagttctgac tcagggcttt ttaacagttc
     8521 aagcaattgt cagttatatt ttggaaactc catctgtgta attctccagt gccttgaaag
     8581 aattattaac ttggcaacac tattaaaact ttataaaaga tggtctttag tgcacgtgta
     8641 tcattatata cacgttttaa agtcatattg cttagcttgt taataatgat tctgcatgtg
     8701 tgctgggttt gggtaattct ttaaaggaag ttttctagat ttgcacttga tgtttgtttt
     8761 ttaaaaactg attatttatg gccgtgacac tgttaccaga aaagtaattc taattaagtt
     8821 attatgcaaa gtcatctata agtagcatct gggaagagga gatcgaggcc acagtttgct
     8881 attttagtat gaaaggagga tctgtttggg aaacatagat tgtcttcccc tcaaatgagg
     8941 ggaaaaaaaa aagacccttt gttcaaatgg attctgttgt aaaaaattat ttttaaagga
     9001 aatcacaaat tgtatgtcat tcttaatgct agtcttatag aataaatcca taaaattgtt
     9061 tttatgttca gtatgtttat gtcattctaa atgcagcaaa ttcaatgata gcagttcaat
     9121 tgactcatag cagtgttttg tattttttct aattctttag ctttcaatat tggattaaag
     9181 tcttgtttgt gaatatagtt tccgtatggc aaatgatttc ttgcttatta gcttttgtta
     9241 aagaatgctt agtaagagct aagcttttaa aagtaatgca aacatttatc gttaataaaa
     9301 cctatggtgt aatatcatat aatgcttttc tttgatcttt ggagaattat tcttttatag
     9361 tagtatacat gaattttgat ttttaaagca tttaaaaaca aatctcaata cattaaaaaa
     9421 cctgttattg ttaaaaagga aattaccatg cctttaagaa acaaggatgt acatcttcaa
     9481 ttcagcataa gtgtccacat ctagaaggct ctcattgcag ttgtttacag ttaaggtacc
     9541 tctatctaaa gggccaaaga agcatttcat attttaacac ctcacattct ttcaggatta
     9601 agacatatga aaatagtctg aataggataa atttgatagg aagtaactta accagtctgg
     9661 aagattcagc tttttctata aaaagcttat tcctcttcac aactcagatg tagatttcat
     9721 tttcaagagg tagatatttt aaagcaatga tctatttggg ttttagtttt atgagtaaat
     9781 cattaattgt atggtttggg ggttatttta ttttccatgt taatataagc acttatttct
     9841 gaagttattg agaggtcatc tagctccagt tcaaaagatt atgtatatct gatgtttaag
     9901 aggaaaaaca tctgaaaaaa tatcttttgt ttatttgaaa aaagcatata tattccaact
     9961 ttaaaattgt gaatttattt tgagtaacct ctaattaact gtattttttt gtttctgttg
    10021 ctgtatagtt agaaatttca tggcaatatt ttttactaaa ttgaaactat aggttttaaa
    10081 aataaagaca ttttagaaaa
//
