LOCUS       NP_065825               1006 aa            linear   PRI 30-APR-2025
DEFINITION  E3 ubiquitin-protein ligase MIB1 [Homo sapiens].
ACCESSION   NP_065825
VERSION     NP_065825.1
DBSOURCE    REFSEQ: accession NM_020774.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1006)
  AUTHORS   Cao,R., Gozlan,O., Airich,A., Tveriakhina,L., Zhou,H., Jiang,H.,
            Cole,P.A., Aster,J.C., Klein,T., Sprinzak,D. and Blacklow,S.C.
  TITLE     Structural requirements for activity of Mind bomb1 in Notch
            signaling
  JOURNAL   Structure 32 (10), 1667-1676 (2024)
   PUBMED   39121852
  REMARK    GeneRIF: Structural requirements for activity of Mind bomb1 in
            Notch signaling.
REFERENCE   2  (residues 1 to 1006)
  AUTHORS   Xu,P., Liu,K., Huang,S., Lv,J., Yan,Z., Ge,H., Cheng,Q., Chen,Z.,
            Ji,P., Qian,Y., Li,B., Xu,H., Yang,L., Xu,Z. and Zhang,D.
  TITLE     N6-methyladenosine-modified MIB1 promotes stemness properties and
            peritoneal metastasis of gastric cancer cells by ubiquitinating
            DDX3X
  JOURNAL   Gastric Cancer 27 (2), 275-291 (2024)
   PUBMED   38252226
  REMARK    GeneRIF: N[6]-methyladenosine-modified MIB1 promotes stemness
            properties and peritoneal metastasis of gastric cancer cells by
            ubiquitinating DDX3X.
REFERENCE   3  (residues 1 to 1006)
  AUTHORS   Tessler,I., Albuisson,J., Pineiro-Sabaris,R., Verstraeten,A.,
            Kamber Kaya,H.E., Siguero-Alvarez,M., Goudot,G., MacGrogan,D.,
            Luyckx,I., Shpitzen,S., Levin,G., Kelman,G., Reshef,N., Mananet,H.,
            Holdcraft,J., Muehlschlegel,J.D., Peloso,G.M., Oppenheim,O.,
            Cheng,C., Mazzella,J.M., Andelfinger,G., Mital,S., Eriksson,P.,
            Billon,C., Heydarpour,M., Dietz,H.C., Jeunemaitre,X.,
            Leitersdorf,E., Sprinzak,D., Blacklow,S.C., Body,S.C., Carmi,S.,
            Loeys,B., de la Pompa,J.L., Gilon,D., Messas,E. and Durst,R.
  TITLE     Novel Association of the NOTCH Pathway Regulator MIB1 Gene With the
            Development of Bicuspid Aortic Valve
  JOURNAL   JAMA Cardiol 8 (8), 721-731 (2023)
   PUBMED   37405741
  REMARK    GeneRIF: Novel Association of the NOTCH Pathway Regulator MIB1 Gene
            With the Development of Bicuspid Aortic Valve.
REFERENCE   4  (residues 1 to 1006)
  AUTHORS   Kanoh,T., Lu,J., Mizoguchi,T. and Itoh,M.
  TITLE     The E3 ubiquitin ligase MIB1 suppresses breast cancer cell
            migration through regulating CTNND1 protein level
  JOURNAL   Biochem Biophys Res Commun 667, 73-80 (2023)
   PUBMED   37209565
  REMARK    GeneRIF: The E3 ubiquitin ligase MIB1 suppresses breast cancer cell
            migration through regulating CTNND1 protein level.
REFERENCE   5  (residues 1 to 1006)
  AUTHORS   Zhang,L., Nephew,K.P. and Gallagher,P.J.
  TITLE     Regulation of death-associated protein kinase. Stabilization by
            HSP90 heterocomplexes
  JOURNAL   J Biol Chem 282 (16), 11795-11804 (2007)
   PUBMED   17324930
  REMARK    GeneRIF: DAPK is found in two distinct immune complexes, one
            containing HSP90 and CHIP and a second complex containing only
            DIP1/Mib; strict modulation of DAPK activities by HSP90
            heterocomplexes is critical for regulation of apoptosis and
            cellular homeostasis
REFERENCE   6  (residues 1 to 1006)
  AUTHORS   Song,R., Koo,B.K., Yoon,K.J., Yoon,M.J., Yoo,K.W., Kim,H.T.,
            Oh,H.J., Kim,Y.Y., Han,J.K., Kim,C.H. and Kong,Y.Y.
  TITLE     Neuralized-2 regulates a Notch ligand in cooperation with Mind
            bomb-1
  JOURNAL   J Biol Chem 281 (47), 36391-36400 (2006)
   PUBMED   17003037
  REMARK    GeneRIF: Neuralized-2 regulates a Notch ligand in cooperation with
            Mind bomb-1
REFERENCE   7  (residues 1 to 1006)
  AUTHORS   Yoo,K.W., Kim,E.H., Jung,S.H., Rhee,M., Koo,B.K., Yoon,K.J.,
            Kong,Y.Y. and Kim,C.H.
  TITLE     Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic
            and endothelial precursor cells in zebrafish
  JOURNAL   FEBS Lett 580 (18), 4409-4416 (2006)
   PUBMED   16857196
REFERENCE   8  (residues 1 to 1006)
  AUTHORS   Henshall,D.C., Schindler,C.K., So,N.K., Lan,J.Q., Meller,R. and
            Simon,R.P.
  TITLE     Death-associated protein kinase expression in human temporal lobe
            epilepsy
  JOURNAL   Ann Neurol 55 (4), 485-494 (2004)
   PUBMED   15048887
REFERENCE   9  (residues 1 to 1006)
  AUTHORS   Itoh,M., Kim,C.H., Palardy,G., Oda,T., Jiang,Y.J., Maust,D.,
            Yeo,S.Y., Lorick,K., Wright,G.J., Ariza-McNaughton,L.,
            Weissman,A.M., Lewis,J., Chandrasekharappa,S.C. and Chitnis,A.B.
  TITLE     Mind bomb is a ubiquitin ligase that is essential for efficient
            activation of Notch signaling by Delta
  JOURNAL   Dev Cell 4 (1), 67-82 (2003)
   PUBMED   12530964
REFERENCE   10 (residues 1 to 1006)
  AUTHORS   Jin,Y., Blue,E.K., Dixon,S., Shao,Z. and Gallagher,P.J.
  TITLE     A death-associated protein kinase (DAPK)-interacting protein,
            DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis
            factor-induced apoptosis and regulates the cellular levels of DAPK
  JOURNAL   J Biol Chem 277 (49), 46980-46986 (2002)
   PUBMED   12351649
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091038.9, AY149908.1 and
            AC103987.5.
            
            Summary: This gene encodes a protein containing multiple ankyrin
            repeats and RING finger domains that functions as an E3 ubiquitin
            ligase. The encoded protein positively regulates Notch signaling by
            ubiquitinating the Notch receptors, thereby facilitating their
            endocytosis. This protein may also promote the ubiquitination and
            degradation of death-associated protein kinase 1 (DAPK1). [provided
            by RefSeq, Jun 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY147849.1, BC110582.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261537.7/ ENSP00000261537.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q11.2"
     Protein         1..1006
                     /product="E3 ubiquitin-protein ligase MIB1"
                     /EC_number="2.3.2.27"
                     /note="ubiquitin ligase mind bomb; DAPK-interacting
                     protein 1; zinc finger ZZ type with ankyrin repeat domain
                     protein 2; RING-type E3 ubiquitin transferase MIB1;
                     mindbomb E3 ubiquitin protein ligase 1"
                     /calculated_mol_wt=110005
     Region          15..72
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          83..127
                     /region_name="ZZ_Mind_bomb"
                     /note="Zinc finger, ZZ type. Zinc finger present in
                     Drosophila Mind bomb (D-mib) and related proteins. The ZZ
                     motif coordinates two zinc ions and most likely
                     participates in ligand binding or molecular scaffolding.
                     Mind bomb is an E3 ubiqitin ligase that has...; cd02339"
                     /db_xref="CDD:239079"
     Site            order(85,88,100,103,109,112,118,122)
                     /site_type="other"
                     /note="Zinc-binding sites [ion binding]"
                     /db_xref="CDD:239079"
     Site            order(85,88,109,112)
                     /site_type="other"
                     /note="zinc cluster 1 [ion binding]"
                     /db_xref="CDD:239079"
     Site            order(86,97,99,105,107)
                     /site_type="active"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239079"
     Site            order(98,113,124,127)
                     /site_type="active"
                     /note="putative hydrophobic binding surface [active]"
                     /db_xref="CDD:239079"
     Site            order(100,103,118,122)
                     /site_type="other"
                     /note="zinc cluster 2 [ion binding]"
                     /db_xref="CDD:239079"
     Region          154..218
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          246..311
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Region          333..397
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Site            408
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q86YT6.1)"
     Region          430..460
                     /region_name="ANK 1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q86YT6.1)"
     Region          444..720
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          463..492
                     /region_name="ANK 2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q86YT6.1)"
     Region          464..494
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          496..560
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          496..525
                     /region_name="ANK 3"
                     /note="propagated from UniProtKB/Swiss-Prot (Q86YT6.1)"
     Region          529..558
                     /region_name="ANK 4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q86YT6.1)"
     Site            order(562,564,568..569,572..574,576..577,581,584,593,631,
                     633,637..638,641..643,645..646,650,653,663,665,667,
                     671..672,675..677,679..680,684,687,696)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          562..593
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          562..591
                     /region_name="ANK 5"
                     /note="propagated from UniProtKB/Swiss-Prot (Q86YT6.1)"
     Region          595..627
                     /region_name="ANK 6"
                     /note="propagated from UniProtKB/Swiss-Prot (Q86YT6.1)"
     Region          631..663
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          631..661
                     /region_name="ANK 7"
                     /note="propagated from UniProtKB/Swiss-Prot (Q86YT6.1)"
     Region          665..696
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          665..694
                     /region_name="ANK 8"
                     /note="propagated from UniProtKB/Swiss-Prot (Q86YT6.1)"
     Region          668..>778
                     /region_name="PHA03095"
                     /note="ankyrin-like protein; Provisional"
                     /db_xref="CDD:222980"
     Region          698..729
                     /region_name="ANK 9"
                     /note="propagated from UniProtKB/Swiss-Prot (Q86YT6.1)"
     Region          817..854
                     /region_name="RING-HC_MIB1_rpt1"
                     /note="first RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16724"
                     /db_xref="CDD:438384"
     Region          864..901
                     /region_name="RING-HC_MIB1_rpt2"
                     /note="second RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16725"
                     /db_xref="CDD:438385"
     Region          961..1006
                     /region_name="RING-HC_MIB1_rpt3"
                     /note="third RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16727"
                     /db_xref="CDD:438387"
     CDS             1..1006
                     /gene="MIB1"
                     /gene_synonym="DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6"
                     /coded_by="NM_020774.4:793..3813"
                     /db_xref="CCDS:CCDS11871.1"
                     /db_xref="GeneID:57534"
                     /db_xref="HGNC:HGNC:21086"
                     /db_xref="MIM:608677"
ORIGIN      
        1 msnsrnnrvm vegvgarvvr gpdwkwgkqd ggeghvgtvr sfespeevvv vwdngtaany
       61 rcsgaydlri ldsaptgikh dgtmcdtcrq qpiigirwkc aectnydlct vcyhgdkhhl
      121 rhrfyrittp gservllesr rkskkitarg ifagarvvrg vdwqwedqdg gngrrgkvte
      181 iqdwsassph saayvlwdng aknlyrvgfe gmsdlkcvqd akggsfyrdh cpvlgeqngn
      241 rnpgglqigd lvnidldlei vqslqhghgg wtdgmfetlt ttgtvcgide dhdivvqyps
      301 gnrwtfnpav ltkanivrsg daaqgaeggt sqfqvgdlvq vcydlerikl lqrghgewae
      361 amlptlgkvg rvqqiysdsd lkvevcgtsw tynpaavskv asagsaisna sgerlsqllk
      421 klfetqesgd lneelvkaaa ngdvakvedl lkrpdvdvng qcaghtamqa asqnghvdil
      481 klllkqnvdv eaedkdgdra vhhaafgdeg avievlhrgs adlnarnkrr qtplhiavnk
      541 ghlqvvktll dfgchpslqd segdtplhda iskkrddila vlleagadvt itnnngfnal
      601 hhaalrgnps amrvllsklp rpwivdekkd dgytalhlaa lnnhvevael lvhqgnanld
      661 iqnvnqqtal hlaverqhtq ivrllvraga kldiqdkdgd tplhealrhh tlsqlrqlqd
      721 mqdvgkvdaa wepskntlim glgtqgaekk saasiacfla angadlsirn kkgqspldlc
      781 pdpnlckala kchkekvsgq vgsrspsmis ndsetleecm vcsdmkrdtl fgpcghiatc
      841 slcsprvkkc lickeqvqsr tkieecvvcs dkkaavlfqp cghmcacenc anlmkkcvqc
      901 ravverrvpf imccggksse datddissgn ipvlqkdkdn tnvnadvqkl qqqlqdikeq
      961 tmcpvcldrl knmiflcghg tcqlcgdrms ecpicrkaie rrilly
//
