LOCUS       NM_002413                740 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens microsomal glutathione S-transferase 2 (MGST2),
            transcript variant 1, mRNA.
ACCESSION   NM_002413
VERSION     NM_002413.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Thulasingam,M., Orellana,L., Nji,E., Ahmad,S., Rinaldo-Matthis,A.
            and Haeggstrom,J.Z.
  TITLE     Crystal structures of human MGST2 reveal synchronized
            conformational changes regulating catalysis
  JOURNAL   Nat Commun 12 (1), 1728 (2021)
   PUBMED   33741927
  REMARK    GeneRIF: Crystal structures of human MGST2 reveal synchronized
            conformational changes regulating catalysis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 740)
  AUTHORS   Ahmad,S., Thulasingam,M., Palombo,I., Daley,D.O., Johnson,K.A.,
            Morgenstern,R., Haeggstrom,J.Z. and Rinaldo-Matthis,A.
  TITLE     Trimeric microsomal glutathione transferase 2 displays one third of
            the sites reactivity
  JOURNAL   Biochim Biophys Acta 1854 (10 Pt A), 1365-1371 (2015)
   PUBMED   26066610
  REMARK    GeneRIF: Trimeric microsomal protein MGST2 displays one third of
            the sites reactivity.
REFERENCE   4  (bases 1 to 740)
  AUTHORS   Sahni,N., Yi,S., Taipale,M., Fuxman Bass,J.I.,
            Coulombe-Huntington,J., Yang,F., Peng,J., Weile,J., Karras,G.I.,
            Wang,Y., Kovacs,I.A., Kamburov,A., Krykbaeva,I., Lam,M.H.,
            Tucker,G., Khurana,V., Sharma,A., Liu,Y.Y., Yachie,N., Zhong,Q.,
            Shen,Y., Palagi,A., San-Miguel,A., Fan,C., Balcha,D., Dricot,A.,
            Jordan,D.M., Walsh,J.M., Shah,A.A., Yang,X., Stoyanova,A.K.,
            Leighton,A., Calderwood,M.A., Jacob,Y., Cusick,M.E.,
            Salehi-Ashtiani,K., Whitesell,L.J., Sunyaev,S., Berger,B.,
            Barabasi,A.L., Charloteaux,B., Hill,D.E., Hao,T., Roth,F.P.,
            Xia,Y., Walhout,A.J.M., Lindquist,S. and Vidal,M.
  TITLE     Widespread macromolecular interaction perturbations in human
            genetic disorders
  JOURNAL   Cell 161 (3), 647-660 (2015)
   PUBMED   25910212
REFERENCE   5  (bases 1 to 740)
  AUTHORS   Ahmad,S., Niegowski,D., Wetterholm,A., Haeggstrom,J.Z.,
            Morgenstern,R. and Rinaldo-Matthis,A.
  TITLE     Catalytic characterization of human microsomal glutathione
            S-transferase 2: identification of rate-limiting steps
  JOURNAL   Biochemistry 52 (10), 1755-1764 (2013)
   PUBMED   23409838
  REMARK    GeneRIF: characterized the substrate specificity and catalytic
            properties of purified MGST2 by steady-state and pre-steady-state
            kinetic experiments
REFERENCE   6  (bases 1 to 740)
  AUTHORS   Jakobsson,P.J., Morgenstern,R., Mancini,J., Ford-Hutchinson,A. and
            Persson,B.
  TITLE     Membrane-associated proteins in eicosanoid and glutathione
            metabolism (MAPEG). A widespread protein superfamily
  JOURNAL   Am J Respir Crit Care Med 161 (2 Pt 2), S20-S24 (2000)
   PUBMED   10673221
  REMARK    Review article
REFERENCE   7  (bases 1 to 740)
  AUTHORS   Jakobsson,P.J., Mancini,J.A., Riendeau,D. and Ford-Hutchinson,A.W.
  TITLE     Identification and characterization of a novel microsomal enzyme
            with glutathione-dependent transferase and peroxidase activities
  JOURNAL   J Biol Chem 272 (36), 22934-22939 (1997)
   PUBMED   9278457
REFERENCE   8  (bases 1 to 740)
  AUTHORS   Jakobsson,P.J., Scoggan,K.A., Yergey,J., Mancini,J.A. and
            Ford-Hutchinson,A.W.
  TITLE     Characterization of microsomal GST-II by western blot and
            identification of a novel LTC4 isomer
  JOURNAL   J Lipid Mediat Cell Signal 17 (1), 15-19 (1997)
   PUBMED   9302651
REFERENCE   9  (bases 1 to 740)
  AUTHORS   Scoggan,K.A., Jakobsson,P.J. and Ford-Hutchinson,A.W.
  TITLE     Production of leukotriene C4 in different human tissues is
            attributable to distinct membrane bound biosynthetic enzymes
  JOURNAL   J Biol Chem 272 (15), 10182-10187 (1997)
   PUBMED   9092565
REFERENCE   10 (bases 1 to 740)
  AUTHORS   Jakobsson,P.J., Mancini,J.A. and Ford-Hutchinson,A.W.
  TITLE     Identification and characterization of a novel human microsomal
            glutathione S-transferase with leukotriene C4 synthase activity and
            significant sequence identity to 5-lipoxygenase-activating protein
            and leukotriene C4 synthase
  JOURNAL   J Biol Chem 271 (36), 22203-22210 (1996)
   PUBMED   8703034
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC112236.3 and AC108053.3.
            
            On Nov 23, 2018 this sequence version replaced NM_002413.4.
            
            Summary: The MAPEG (Membrane Associated Proteins in Eicosanoid and
            Glutathione metabolism) family consists of six human proteins,
            several of which are involved in the production of leukotrienes and
            prostaglandin E, important mediators of inflammation. This gene
            encodes a protein which catalyzes the conjugation of leukotriene A4
            and reduced glutathione to produce leukotriene C4. Alternatively
            spliced transcript variants encoding different isoforms have been
            identified in this gene. [provided by RefSeq, Feb 2011].
            
            Transcript Variant: This variant (1) is a predominant transcript
            and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK130948.1, SRR5189655.24472.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265498.6/ ENSP00000265498.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-259               AC112236.3         125581-125839
            260-359             AC112236.3         138305-138404
            360-430             AC112236.3         154959-155029
            431-512             AC108053.3         4600-4681
            513-740             AC108053.3         5161-5388
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q31.1"
     gene            1..740
                     /gene="MGST2"
                     /gene_synonym="GST2; MGST-II"
                     /note="microsomal glutathione S-transferase 2"
                     /db_xref="GeneID:4258"
                     /db_xref="HGNC:HGNC:7063"
                     /db_xref="MIM:601733"
     exon            1..259
                     /gene="MGST2"
                     /gene_synonym="GST2; MGST-II"
                     /inference="alignment:Splign:2.1.0"
     CDS             202..645
                     /gene="MGST2"
                     /gene_synonym="GST2; MGST-II"
                     /EC_number="2.5.1.18"
                     /EC_number="4.4.1.20"
                     /note="isoform 1 is encoded by transcript variant 1;
                     microsomal GST-2; microsomal GST-II; glutathione
                     peroxidase MGST2; leukotriene C4 synthase MGST2;
                     microsomal glutathione S-transferase II"
                     /codon_start=1
                     /product="microsomal glutathione S-transferase 2 isoform
                     1"
                     /protein_id="NP_002404.1"
                     /db_xref="CCDS:CCDS3749.1"
                     /db_xref="GeneID:4258"
                     /db_xref="HGNC:HGNC:7063"
                     /db_xref="MIM:601733"
                     /translation="MAGNSILLAAVSILSACQQSYFALQVGKARLKYKVTPPAVTGSP
                     EFERVFRAQQNCVEFYPIFIITLWMAGWYFNQVFATCLGLVYIYGRHLYFWGYSEAAK
                     KRITGFRLSLGILALLTLLGALGIANSFLDEYLDLNIAKKLRRQF"
     exon            260..359
                     /gene="MGST2"
                     /gene_synonym="GST2; MGST-II"
                     /inference="alignment:Splign:2.1.0"
     exon            360..430
                     /gene="MGST2"
                     /gene_synonym="GST2; MGST-II"
                     /inference="alignment:Splign:2.1.0"
     exon            431..512
                     /gene="MGST2"
                     /gene_synonym="GST2; MGST-II"
                     /inference="alignment:Splign:2.1.0"
     exon            513..740
                     /gene="MGST2"
                     /gene_synonym="GST2; MGST-II"
                     /inference="alignment:Splign:2.1.0"
     regulatory      715..720
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGST2"
                     /gene_synonym="GST2; MGST-II"
                     /note="hexamer: AATAAA"
     polyA_site      740
                     /gene="MGST2"
                     /gene_synonym="GST2; MGST-II"
ORIGIN      
        1 ataaagatct gtgaccggca gccccagacc tgcctgcctt cctgacttct gttccagagc
       61 aaaggtcatt cagccgcttg aatcagcctt ttccccccac ccggtcccca actttgttta
      121 cccgataagg aaggtcagca ttcaaagtca agaagcgcca tttatcttcc cgtgcgctct
      181 acaaatagtt ccgtgagaaa gatggccggg aactcgatcc tgctggctgc tgtctctatt
      241 ctctcggcct gtcagcaaag ttattttgct ttgcaagttg gaaaggcaag attaaaatac
      301 aaagttacgc ccccagcagt cactgggtca ccagagtttg agagagtatt tcgggcacaa
      361 caaaactgtg tggagtttta tcctatattc ataattacat tgtggatggc tgggtggtat
      421 ttcaaccaag tttttgctac ttgtctgggt ctggtgtaca tatatggccg tcacctatac
      481 ttctggggat attcagaagc tgctaaaaaa cggatcaccg gtttccgact gagtctgggg
      541 attttggcct tgttgaccct cctaggtgcc ctgggaattg caaacagctt tctggatgaa
      601 tatctggacc tcaatattgc caagaaactg aggcggcaat tctaactttt tctcttccct
      661 ttaatacttg cagaagctgt tcccaccatg aaggtaatat ggtatcattt gttaaataaa
      721 aataaagtct ttattctgtt
//
