LOCUS       NM_020300                925 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens microsomal glutathione S-transferase 1 (MGST1),
            transcript variant 2, mRNA.
ACCESSION   NM_020300
VERSION     NM_020300.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Zhang,J., Ye,Z.W., Brautigam,L., Chakraborty,P., Luo,Z.,
            Culpepper,J., Aslam,M., Zhang,L., Johansson,K., Haeggstrom,J.Z.,
            Xu,J., Olsson,M., Townsend,D.M., Mehrotra,S., Morgenstern,R. and
            Tew,K.D.
  TITLE     A role for microsomal glutathione transferase 1 in melanin
            biosynthesis and melanoma progression
  JOURNAL   J Biol Chem 299 (8), 104920 (2023)
   PUBMED   37321450
  REMARK    GeneRIF: A role for microsomal glutathione transferase 1 in melanin
            biosynthesis and melanoma progression.
REFERENCE   2  (bases 1 to 925)
  AUTHORS   Peng,Z. and Peng,N.
  TITLE     Microsomal glutathione S-transferase 1 targets the autophagy
            signaling pathway to suppress ferroptosis in gastric carcinoma
            cells
  JOURNAL   Hum Exp Toxicol 42, 9603271231172915 (2023)
   PUBMED   37161854
  REMARK    GeneRIF: Microsomal glutathione S-transferase 1 targets the
            autophagy signaling pathway to suppress ferroptosis in gastric
            carcinoma cells.
REFERENCE   3  (bases 1 to 925)
  AUTHORS   Yang,B., Xia,S., Ye,X., Jing,W. and Wu,B.
  TITLE     MiR-379-5p targets microsomal glutathione transferase 1 (MGST1) to
            regulate human glioma in cell proliferation, migration and invasion
            and epithelial-mesenchymal transition (EMT)
  JOURNAL   Biochem Biophys Res Commun 568, 8-14 (2021)
   PUBMED   34171541
  REMARK    GeneRIF: MiR-379-5p targets microsomal glutathione transferase 1
            (MGST1) to regulate human glioma in cell proliferation, migration
            and invasion and epithelial-mesenchymal transition (EMT).
REFERENCE   4  (bases 1 to 925)
  AUTHORS   Kuang,F., Liu,J., Xie,Y., Tang,D. and Kang,R.
  TITLE     MGST1 is a redox-sensitive repressor of ferroptosis in pancreatic
            cancer cells
  JOURNAL   Cell Chem Biol 28 (6), 765-775 (2021)
   PUBMED   33539732
  REMARK    GeneRIF: MGST1 is a redox-sensitive repressor of ferroptosis in
            pancreatic cancer cells.
REFERENCE   5  (bases 1 to 925)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 925)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
REFERENCE   7  (bases 1 to 925)
  AUTHORS   Soderstrom,M., Morgenstern,R. and Hammarstrom,S.
  TITLE     Protein-protein interaction affinity chromatography of leukotriene
            C4 synthase
  JOURNAL   Protein Expr Purif 6 (3), 352-356 (1995)
   PUBMED   7663172
REFERENCE   8  (bases 1 to 925)
  AUTHORS   Cholon,A., Giaccia,A.J., Lewis,A.D., Hickson,I. and Brown,J.M.
  TITLE     What role do glutathione S-transferases play in the cellular
            response to ionizing radiation?
  JOURNAL   Int J Radiat Oncol Biol Phys 22 (4), 759-763 (1992)
   PUBMED   1544849
REFERENCE   9  (bases 1 to 925)
  AUTHORS   DeJong,J.L., Mohandas,T. and Tu,C.P.
  TITLE     The gene for the microsomal glutathione S-transferase is on human
            chromosome 12
  JOURNAL   Genomics 6 (2), 379-382 (1990)
   PUBMED   2307478
REFERENCE   10 (bases 1 to 925)
  AUTHORS   DeJong,J.L., Morgenstern,R., Jornvall,H., DePierre,J.W. and Tu,C.P.
  TITLE     Gene expression of rat and human microsomal glutathione
            S-transferases
  JOURNAL   J Biol Chem 263 (17), 8430-8436 (1988)
   PUBMED   3372534
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG617746.1, BC005923.1 and
            AW166965.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020300.4.
            
            Summary: The MAPEG (Membrane Associated Proteins in Eicosanoid and
            Glutathione metabolism) family consists of six human proteins, two
            of which are involved in the production of leukotrienes and
            prostaglandin E, important mediators of inflammation. Other family
            members, demonstrating glutathione S-transferase and peroxidase
            activities, are involved in cellular defense against toxic,
            carcinogenic, and pharmacologically active electrophilic compounds.
            This gene encodes a protein that catalyzes the conjugation of
            glutathione to electrophiles and the reduction of lipid
            hydroperoxides. This protein is localized to the endoplasmic
            reticulum and outer mitochondrial membrane where it is thought to
            protect these membranes from oxidative stress. Several transcript
            variants, some non-protein coding and some protein coding, have
            been found for this gene. [provided by RefSeq, May 2012].
            
            Transcript Variant: This variant (2, also called 1b) differs in the
            5' UTR compared to variant 1. Variants 1-5 all encode the same
            isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.26820.1,
                                           SRR5189658.3526.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000396210.8/ ENSP00000379513.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-490               BG617746.1         4-493
            491-923             BC005923.1         461-893
            924-925             AW166965.1         18-19               c
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.3"
     gene            1..925
                     /gene="MGST1"
                     /gene_synonym="GST12; MGST; MGST-I; PMAN"
                     /note="microsomal glutathione S-transferase 1"
                     /db_xref="GeneID:4257"
                     /db_xref="HGNC:HGNC:7061"
                     /db_xref="MIM:138330"
     exon            1..66
                     /gene="MGST1"
                     /gene_synonym="GST12; MGST; MGST-I; PMAN"
                     /inference="alignment:Splign:2.1.0"
     exon            67..214
                     /gene="MGST1"
                     /gene_synonym="GST12; MGST; MGST-I; PMAN"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..556
                     /gene="MGST1"
                     /gene_synonym="GST12; MGST; MGST-I; PMAN"
                     /EC_number="2.5.1.18"
                     /note="isoform a is encoded by transcript variant 2;
                     glutathione S-transferase 12; microsomal GST-1; microsomal
                     GST-I; peritoneal metastasis associated long noncoding
                     RNA"
                     /codon_start=1
                     /product="microsomal glutathione S-transferase 1 isoform
                     a"
                     /protein_id="NP_064696.1"
                     /db_xref="CCDS:CCDS8677.1"
                     /db_xref="GeneID:4257"
                     /db_xref="HGNC:HGNC:7061"
                     /db_xref="MIM:138330"
                     /translation="MVDLTQVMDDEVFMAFASYATIILSKMMLMSTATAFYRLTRKVF
                     ANPEDCVAFGKGENAKKYLRTDDRVERVRRAHLNDLENIIPFLGIGLLYSLSGPDPST
                     AILHFRLFVGARIYHTIAYLTPLPQPNRALSFFVGYGVTLSMAYRLLKSKLYL"
     exon            215..309
                     /gene="MGST1"
                     /gene_synonym="GST12; MGST; MGST-I; PMAN"
                     /inference="alignment:Splign:2.1.0"
     exon            310..925
                     /gene="MGST1"
                     /gene_synonym="GST12; MGST; MGST-I; PMAN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      905..910
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGST1"
                     /gene_synonym="GST12; MGST; MGST-I; PMAN"
                     /note="hexamer: AATAAA"
     polyA_site      925
                     /gene="MGST1"
                     /gene_synonym="GST12; MGST; MGST-I; PMAN"
                     /note="major polyA site"
ORIGIN      
        1 agtccctgca ttgcgcgcga cccggcggcg ggacaggctt gctgcttcct cctcctcggc
       61 ctcaccattc cagaccaaaa ttgaaaaaat ggttgacctc acccaggtaa tggatgatga
      121 agtattcatg gcttttgcat cctatgcaac aattattctt tcaaaaatga tgcttatgag
      181 tactgcaact gcattctata gattgacaag aaaggttttt gccaatccag aagactgtgt
      241 agcatttggc aaaggagaaa atgccaagaa gtatcttcga acagatgaca gagtagaacg
      301 tgtacgcaga gcccacctga atgaccttga aaatattatt ccatttcttg gaattggcct
      361 cctgtattcc ttgagtggtc ccgacccctc tacagccatc ctgcacttca gactatttgt
      421 cggagcacgg atctaccaca ccattgcata tttgacaccc cttccccagc caaatagagc
      481 tttgagtttt tttgttggat atggagttac tctttccatg gcttacaggt tgctgaaaag
      541 taaattgtac ctgtaaagaa aatcatacaa ctcagcatcc agttggcttt ttaagaattc
      601 tgtacttcca atttataatg aatactttct tagattttag gtaggagggg agcagaggaa
      661 ttatgaactg gggtaaaccc attttgaata ttagcattgc caatatcctg tattcttgtt
      721 ttacatttgg attagaaatt taacatagta attcttaagt cttttgtctg atttttaaag
      781 tactttctta taaatttgga tcatgttatg atttgtaaca ttcacacaac acctcacttt
      841 tgaatctata aaagaattgc acgtatgaga aacctatatt tcaatactgc tgaaacagac
      901 atgaaataaa gaatttaaag aatga
//
