LOCUS       NM_000900               1650 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens matrix Gla protein (MGP), transcript variant 2, mRNA.
ACCESSION   NM_000900
VERSION     NM_000900.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Caiado,H., Cancela,M.L. and Conceicao,N.
  TITLE     Assessment of MGP gene expression in cancer and contribution to
            prognosis
  JOURNAL   Biochimie 214 (Pt B), 49-60 (2023)
   PUBMED   37307958
  REMARK    GeneRIF: Assessment of MGP gene expression in cancer and
            contribution to prognosis.
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Li,X., Xu,X., Liu,L., Tian,Y., Gao,Y., Zhu,G., Lou,S., Zhong,W.,
            Li,D. and Pan,Y.
  TITLE     lncRNA MIR31HG Regulates Proliferation and Migration by Targeting
            Matrix Gla Protein in Nonsyndromic Cleft Lip With or Without Cleft
            Palate
  JOURNAL   DNA Cell Biol 42 (7), 390-398 (2023)
   PUBMED   37327028
  REMARK    GeneRIF: lncRNA MIR31HG Regulates Proliferation and Migration by
            Targeting Matrix Gla Protein in Nonsyndromic Cleft Lip With or
            Without Cleft Palate.
REFERENCE   3  (bases 1 to 1650)
  AUTHORS   Yuan,W., Liu,W., Zhang,Y., Wang,X., Xu,C., Li,Q., Ji,P., Wang,J.,
            Feng,P., Wu,Y., Shen,H. and Wang,P.
  TITLE     Reduced APPL1 impairs osteogenic differentiation of mesenchymal
            stem cells by facilitating MGP expression to disrupt the BMP2
            pathway in osteoporosis
  JOURNAL   J Biol Chem 299 (6), 104823 (2023)
   PUBMED   37187293
  REMARK    GeneRIF: Reduced APPL1 impairs osteogenic differentiation of
            mesenchymal stem cells by facilitating MGP expression to disrupt
            the BMP2 pathway in osteoporosis.
REFERENCE   4  (bases 1 to 1650)
  AUTHORS   Nieddu,V., Melocchi,V., Battistini,C., Franciosa,G., Lupia,M.,
            Stellato,C., Bertalot,G., Olsen,J.V., Colombo,N., Bianchi,F. and
            Cavallaro,U.
  TITLE     Matrix Gla Protein drives stemness and tumor initiation in ovarian
            cancer
  JOURNAL   Cell Death Dis 14 (3), 220 (2023)
   PUBMED   36977707
  REMARK    GeneRIF: Matrix Gla Protein drives stemness and tumor initiation in
            ovarian cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1650)
  AUTHORS   Xing,Y.M., Li,P.S. and Liu,Y.
  TITLE     1,25-(OH)2D3 participates and modulates airway remodeling by
            reducing MGP and TGF-beta1 expression in TNF-alpha-induced airway
            smooth muscle cells
  JOURNAL   Adv Clin Exp Med 31 (2), 151-155 (2022)
   PUBMED   35025148
  REMARK    GeneRIF: 1,25-(OH)2D3 participates and modulates airway remodeling
            by reducing MGP and TGF-beta1 expression in TNF-alpha-induced
            airway smooth muscle cells.
REFERENCE   6  (bases 1 to 1650)
  AUTHORS   Price,P.A., Rice,J.S. and Williamson,M.K.
  TITLE     Conserved phosphorylation of serines in the Ser-X-Glu/Ser(P)
            sequences of the vitamin K-dependent matrix Gla protein from shark,
            lamb, rat, cow, and human
  JOURNAL   Protein Sci 3 (5), 822-830 (1994)
   PUBMED   8061611
REFERENCE   7  (bases 1 to 1650)
  AUTHORS   Hale,J.E., Williamson,M.K. and Price,P.A.
  TITLE     Carboxyl-terminal proteolytic processing of matrix Gla protein
  JOURNAL   J Biol Chem 266 (31), 21145-21149 (1991)
   PUBMED   1939157
REFERENCE   8  (bases 1 to 1650)
  AUTHORS   Cancela,L., Hsieh,C.L., Francke,U. and Price,P.A.
  TITLE     Molecular structure, chromosome assignment, and promoter
            organization of the human matrix Gla protein gene
  JOURNAL   J Biol Chem 265 (25), 15040-15048 (1990)
   PUBMED   2394711
REFERENCE   9  (bases 1 to 1650)
  AUTHORS   Chen,L., O'Bryan,J.P., Smith,H.S. and Liu,E.
  TITLE     Overexpression of matrix Gla protein mRNA in malignant human breast
            cells: isolation by differential cDNA hybridization
  JOURNAL   Oncogene 5 (9), 1391-1395 (1990)
   PUBMED   2216462
REFERENCE   10 (bases 1 to 1650)
  AUTHORS   Kiefer,M.C., Bauer,D.M., Young,D., Hermsen,K.M., Masiarz,F.R. and
            Barr,P.J.
  TITLE     The cDNA and derived amino acid sequences for human and bovine
            matrix Gla protein
  JOURNAL   Nucleic Acids Res 16 (11), 5213 (1988)
   PUBMED   3387234
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC007655.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_000900.4.
            
            Summary: This gene encodes a member of the osteocalcin/matrix Gla
            family of proteins. The encoded vitamin K-dependent protein is
            secreted by chondrocytes and vascular smooth muscle cells, and
            functions as a physiological inhibitor of ectopic tissue
            calcification. Carboxylation status of the encoded protein is
            associated with calcification of the vasculature in human patients
            with cardiovascular disease and calcification of the synovial
            membranes in osteoarthritis patients. Mutations in this gene cause
            Keutel syndrome in human patients, which is characterized by
            abnormal cartilage calcification, peripheral pulmonary stenosis and
            facial hypoplasia. [provided by RefSeq, Sep 2016].
            
            Transcript Variant: This variant (2) represents the shorter
            transcript and encodes the shorter isoform (2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.283314.1, BC093078.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000539261.6/ ENSP00000445907.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-124               AC007655.1         96456-96579         c
            125-157             AC007655.1         94938-94970         c
            158-233             AC007655.1         93697-93772         c
            234-1650            AC007655.1         91589-93005         c
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.3"
     gene            1..1650
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
                     /note="matrix Gla protein"
                     /db_xref="GeneID:4256"
                     /db_xref="HGNC:HGNC:7060"
                     /db_xref="MIM:154870"
     exon            1..124
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..375
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
                     /note="isoform 2 preproprotein is encoded by transcript
                     variant 2; cell growth-inhibiting gene 36 protein"
                     /codon_start=1
                     /product="matrix Gla protein isoform 2 preproprotein"
                     /protein_id="NP_000891.2"
                     /db_xref="CCDS:CCDS8669.1"
                     /db_xref="GeneID:4256"
                     /db_xref="HGNC:HGNC:7060"
                     /db_xref="MIM:154870"
                     /translation="MKSLILLAILAALAVVTLCYESHESMESYELNPFINRRNANTFI
                     SPQQRWRAKVQERIRERSKPVHELNREACDDYRLCERYAMVYGYNAAYNRYFRKRRGT
                     K"
     exon            125..157
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
                     /inference="alignment:Splign:2.1.0"
     exon            158..233
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
                     /inference="alignment:Splign:2.1.0"
     exon            234..1650
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      598..603
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
                     /note="hexamer: AATAAA"
     polyA_site      623
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
                     /note="major polyA site"
     regulatory      1587..1592
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
                     /note="hexamer: TATAAA"
     polyA_site      1609
                     /gene="MGP"
                     /gene_synonym="GIG36; MGLAP; NTI"
ORIGIN      
        1 gtaggagcct ctctccctac tgctgctaca caagaccctg agactgacct gcaggacgaa
       61 accatgaaga gcctgatcct tcttgccatc ctggccgcct tagcggtagt aactttgtgt
      121 tatgaatcac atgaaagcat ggaatcttat gaacttaatc ccttcattaa caggagaaat
      181 gcaaatacct tcatatcccc tcagcagaga tggagagcta aagtccaaga gaggatccga
      241 gaacgctcta agcctgtcca cgagctcaat agggaagcct gtgatgacta cagactttgc
      301 gaacgctacg ccatggttta tggatacaat gctgcctata atcgctactt caggaagcgc
      361 cgagggacca aatgagactg agggaagaaa aaaaatctct ttttttctgg aggctggcac
      421 ctgattttgt atccccctgt agcagcatta cagaaataca taggcttata tacaatgctt
      481 ctttcctgta tattctcttg tctggctgca cccctttttc ccgcccccag attgataagt
      541 aatgaaagtg cactgcagtg agggtcaaag gagagtcaac atatgtgatt gttccataat
      601 aaacttctgg tgtgatactt tcatcttgta aatctgcttt cttttgggaa gatattgaga
      661 tatttaaatc atggcccacc ttacccaaaa taggagattc tgttcatctc atatctagta
      721 ttaattagaa aaataactac ataaaaagaa ggaagctaag aaggcactca ctcagccata
      781 aattctctaa accctctcta ccttggaatc cgtgaatgga atctggtatg ttttttgcag
      841 gattttccta ttgtaaattg tggcaaatac agggctccct tcatttgctt ttcatctctt
      901 atgcatcaaa gtcaaaaaca tttctgaatc aagataatct agaagagaaa aaaggaggaa
      961 aaggaaaaga gaaagcagaa gggacaaata aaagcaattg gcaaaaactg tcaataatag
     1021 tttatacact taactatatc aataatcaca ttatatgtaa atagtctaaa caatccaatt
     1081 attttttact tctacttatg ttatattttt acttctacat ttgttaaagg ttccacgcta
     1141 catttttact attcttgctt taaataattt taataatttc ttttaaagtt tagataataa
     1201 gaaaatatcc cgggccaggc acagtggcca cacctgtaat ctcagtagcc atgaccatac
     1261 caatgcactc cagcctgtga aacagagtga gactctgtct ctacagaaaa ataaaaaaga
     1321 aaagaaagaa aagatctcat atatttaccc atgtaatttt catttcctgt tttcttcatt
     1381 cttctttcca tctggtgtca cttcctttct gcctgacgac ttcctttaac gttttttata
     1441 gttcaggtct gcaggattct ttaagttttg tatgcttgtt tttattcttg aaagatattt
     1501 tcactgtata ttgaatccca agttgccacg tttcttttaa ttattttaag gtattccact
     1561 gaattctagc ttgcacaatt tttcgatata aaatttgttg taattcttac ctttgttcca
     1621 ctgtgtatac tgcatctttt atctctggct
//
