LOCUS       NM_002410               8332 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens alpha-1,6-mannosylglycoprotein
            6-beta-N-acetylglucosaminyltransferase (MGAT5), transcript variant
            2, mRNA.
ACCESSION   NM_002410 NR_024275
VERSION     NM_002410.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Xi,X., Yang,Y., Chen,Q., Ma,J., Wang,X., Deng,Y., Wang,X. and Li,Y.
  TITLE     GnT-V-mediated aberrant N-glycosylation of TIMP-1 promotes diabetic
            retinopathy progression
  JOURNAL   Mol Biol Rep 51 (1), 428 (2024)
   PUBMED   38499842
  REMARK    GeneRIF: GnT-V-mediated aberrant N-glycosylation of TIMP-1 promotes
            diabetic retinopathy progression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   de-Souza-Ferreira,M., Ferreira,E.E. and de-Freitas-Junior,J.C.M.
  TITLE     Aberrant N-glycosylation in cancer: MGAT5 and beta1,6-GlcNAc
            branched N-glycans as critical regulators of tumor development and
            progression
  JOURNAL   Cell Oncol (Dordr) 46 (3), 481-501 (2023)
   PUBMED   36689079
  REMARK    GeneRIF: Aberrant N-glycosylation in cancer: MGAT5 and
            beta1,6-GlcNAc branched N-glycans as critical regulators of tumor
            development and progression.
            Review article
REFERENCE   3  (bases 1 to 8332)
  AUTHORS   Osuka,R.F., Hirata,T., Nagae,M., Nakano,M., Shibata,H., Okamoto,R.
            and Kizuka,Y.
  TITLE     N-acetylglucosaminyltransferase-V requires a specific noncatalytic
            luminal domain for its activity toward glycoprotein substrates
  JOURNAL   J Biol Chem 298 (3), 101666 (2022)
   PUBMED   35104505
  REMARK    GeneRIF: N-acetylglucosaminyltransferase-V requires a specific
            noncatalytic luminal domain for its activity toward glycoprotein
            substrates.
REFERENCE   4  (bases 1 to 8332)
  AUTHORS   Marhuenda,E., Fabre,C., Zhang,C., Martin-Fernandez,M.,
            Iskratsch,T., Saleh,A., Bauchet,L., Cambedouzou,J., Hugnot,J.P.,
            Duffau,H., Dennis,J.W., Cornu,D. and Bakalara,N.
  TITLE     Glioma stem cells invasive phenotype at optimal stiffness is driven
            by MGAT5 dependent mechanosensing
  JOURNAL   J Exp Clin Cancer Res 40 (1), 139 (2021)
   PUBMED   33894774
  REMARK    GeneRIF: Glioma stem cells invasive phenotype at optimal stiffness
            is driven by MGAT5 dependent mechanosensing.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8332)
  AUTHORS   Li,X., Zhou,G., Tian,X., Chen,F., Li,G. and Ding,Y.
  TITLE     The polymorphisms of FGFR2 and MGAT5 affect the susceptibility to
            COPD in the Chinese people
  JOURNAL   BMC Pulm Med 21 (1), 129 (2021)
   PUBMED   33879098
  REMARK    GeneRIF: The polymorphisms of FGFR2 and MGAT5 affect the
            susceptibility to COPD in the Chinese people.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8332)
  AUTHORS   Granovsky,M., Fata,J., Pawling,J., Muller,W.J., Khokha,R. and
            Dennis,J.W.
  TITLE     Suppression of tumor growth and metastasis in Mgat5-deficient mice
  JOURNAL   Nat Med 6 (3), 306-312 (2000)
   PUBMED   10700233
REFERENCE   7  (bases 1 to 8332)
  AUTHORS   Dennis,J.W., Granovsky,M. and Warren,C.E.
  TITLE     Glycoprotein glycosylation and cancer progression
  JOURNAL   Biochim Biophys Acta 1473 (1), 21-34 (1999)
   PUBMED   10580127
  REMARK    Review article
REFERENCE   8  (bases 1 to 8332)
  AUTHORS   Park,C., Jin,U.H., Lee,Y.C., Cho,T.J. and Kim,C.H.
  TITLE     Characterization of UDP-N-acetylglucosamine:alpha-6-d-mannoside
            beta-1,6-N-acetylglucosaminyltransferase V from a human hepatoma
            cell line Hep3B
  JOURNAL   Arch Biochem Biophys 367 (2), 281-288 (1999)
   PUBMED   10395745
REFERENCE   9  (bases 1 to 8332)
  AUTHORS   Buckhaults,P., Chen,L., Fregien,N. and Pierce,M.
  TITLE     Transcriptional regulation of N-acetylglucosaminyltransferase V by
            the src oncogene
  JOURNAL   J Biol Chem 272 (31), 19575-19581 (1997)
   PUBMED   9235963
REFERENCE   10 (bases 1 to 8332)
  AUTHORS   Saito,H., Nishikawa,A., Gu,J., Ihara,Y., Soejima,H., Wada,Y.,
            Sekiya,C., Niikawa,N. and Taniguchi,N.
  TITLE     cDNA cloning and chromosomal mapping of human
            N-acetylglucosaminyltransferase V+
  JOURNAL   Biochem Biophys Res Commun 198 (1), 318-327 (1994)
   PUBMED   8292036
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016906.7, D17716.1,
            AC013718.6, BU191961.1, BX647087.1, DB237089.1, DB223638.1 and
            AK056461.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002410.4.
            
            Summary: The protein encoded by this gene belongs to the
            glycosyltransferase family. It catalyzes the addition of
            beta-1,6-N-acetylglucosamine to the alpha-linked mannose of
            biantennary N-linked oligosaccharides present on the newly
            synthesized glycoproteins. It is one of the most important enzymes
            involved in the regulation of the biosynthesis of glycoprotein
            oligosaccharides. Alterations of the oligosaccharides on cell
            surface glycoproteins cause significant changes in the adhesive or
            migratory behavior of a cell. Increase in the activity of this
            enzyme has been correlated with the progression of invasive
            malignancies. [provided by RefSeq, Oct 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D17716.1, AF113921.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281923.4/ ENSP00000281923.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-190               AC016906.7         73804-73993
            191-2608            D17716.1           4-2421
            2609-3004           AC013718.6         36191-36586
            3005-3642           BU191961.1         1-638
            3643-4165           BX647087.1         614-1136
            4166-4509           DB237089.1         152-495
            4510-5006           DB223638.1         10-506
            5007-6407           BX647087.1         1976-3376
            6408-8331           AK056461.1         834-2757
            8332-8332           BX647087.1         5301-5301
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q21.2-q21.3"
     gene            1..8332
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /note="alpha-1,6-mannosylglycoprotein
                     6-beta-N-acetylglucosaminyltransferase"
                     /db_xref="GeneID:4249"
                     /db_xref="HGNC:HGNC:7049"
                     /db_xref="MIM:601774"
     exon            1..573
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     CDS             333..2558
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /EC_number="2.4.1.155"
                     /note="N-acetylglucosaminyl-transferase V; mannosyl
                     (alpha-1,6-)-glycoprotein
                     beta-1,6-N-acetyl-glucosaminyltransferase; mannoside
                     acetylglucosaminyltransferase 5; alpha-mannoside
                     beta-1,6-N-acetylglucosaminyltransferase V"
                     /codon_start=1
                     /product="alpha-1,6-mannosylglycoprotein
                     6-beta-N-acetylglucosaminyltransferase A"
                     /protein_id="NP_002401.1"
                     /db_xref="CCDS:CCDS2171.1"
                     /db_xref="GeneID:4249"
                     /db_xref="HGNC:HGNC:7049"
                     /db_xref="MIM:601774"
                     /translation="MALFTPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQPESSS
                     MLREQILDLSKRYIKALAEENRNVVDGPYAGVMTAYDLKKTLAVLLDNILQRIGKLES
                     KVDNLVVNGTGTNSTNSTTAVPSLVALEKINVADIINGAQEKCVLPPMDGYPHCEGKI
                     KWMKDMWRSDPCYADYGVDGSTCSFFIYLSEVENWCPHLPWRAKNPYEEADHNSLAEI
                     RTDFNILYSMMKKHEEFRWMRLRIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGL
                     LTKESGFKIAETAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGN
                     RSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYA
                     QSKGHKTPWGKWNLNPQQFYTMFPHTPDNSFLGFVVEQHLNSSDIHHINEIKRQNQSL
                     VYGKVDSFWKNKKIYLDIIHTYMEVHATVYGSSTKNIPSYVKNHGILSGRDLQFLLRE
                     TKLFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRELTSQHPY
                     AEVFIGRPHVWTVDLNNQEEVEDAVKAILNQKIEPYMPYEFTCEGMLQRINAFIEKQD
                     FCHGQVMWPPLSALQVKLAEPGQSCKQVCQESQLICEPSFFQHLNKDKDMLKYKVTCQ
                     SSELAKDILVPSFDPKNKHCVFQGDLLLFSCAGAHPRHQRVCPCRDFIKGQVALCKDC
                     L"
     exon            574..738
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            739..815
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            816..905
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            906..977
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1139
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1309
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1444
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1578
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1712
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1862
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1863..2009
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2126
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            2127..2201
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2359
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..8332
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8313..8318
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /note="hexamer: AATAAA"
     polyA_site      8332
                     /gene="MGAT5"
                     /gene_synonym="glcNAc-T V; GNT-V; GNT-VA; MGAT5A"
                     /note="major polyA site"
ORIGIN      
        1 ataattgctt agcttcttgt tgcctagcca gatttcccct cagcttacag ttcctgaatc
       61 ataagatatt gaaccagcaa atttaagagt tgacatttta cttagaggta ttcaagtgaa
      121 aacatggctt ctggtttatt ttgctgtatt gtgccatgac cacttggcta attcttctcc
      181 tccttcacag cagaatggaa gtgaggaaag gcaaccagct gacacaggag ccagagtgag
      241 accagcagac tctcacactc aacctacacc atgaatttgt gtctatcttc tacgcgttaa
      301 gagccaagga caggtgaagt tgccagagag caatggctct cttcactccg tggaagttgt
      361 cctctcagaa gctgggcttt ttcctggtga cttttggctt catttggggt atgatgcttc
      421 tgcactttac catccagcag cgaactcagc ctgaaagcag ctccatgctg cgcgagcaga
      481 tcctggacct cagcaaaagg tacatcaagg cactggcaga agaaaacagg aatgtggtgg
      541 atgggccata cgctggagtc atgacagctt atgatctgaa gaaaaccctt gctgtgttat
      601 tagataacat tttgcagcgc attggcaagt tggagtcgaa ggtggacaat cttgttgtca
      661 atggcaccgg aacaaactca accaactcca ctacagctgt tcccagcttg gttgcacttg
      721 agaaaattaa tgtggcagat atcattaacg gagctcaaga aaaatgtgta ttgcctccta
      781 tggacggcta ccctcactgt gagggaaaga tcaagtggat gaaagacatg tggcgttcag
      841 atccctgcta cgcagactat ggagtggatg gatccacctg ctcttttttt atttacctca
      901 gtgaggttga aaattggtgt cctcatttac cttggagagc aaaaaatccc tacgaagaag
      961 ctgatcataa ttcattggcg gaaattcgta cagattttaa tattctctac agtatgatga
     1021 aaaagcatga agaattccgg tggatgagac tacggatccg gcgaatggct gacgcatgga
     1081 tccaagcaat caagtccctg gcagaaaagc agaaccttga aaagagaaag cggaagaaag
     1141 tcctcgttca cctgggactc ctgaccaagg aatctggatt taagattgca gagacagctt
     1201 tcagtggtgg ccctcttggt gaattagttc aatggagtga tttaattaca tctctgtact
     1261 tactgggcca tgacattagg atttcagctt cactggctga gctcaaggaa atcatgaaga
     1321 aggttgtagg aaaccgatct ggctgcccaa ctgtaggaga cagaattgtt gagctcattt
     1381 acattgatat tgtaggactt gctcaattca agaaaactct tggaccatcc tgggttcatt
     1441 accagtgcat gctccgagtc cttgattcat ttggtactga acccgaattt aatcatgcaa
     1501 attatgccca atcgaaaggc cacaagaccc cttggggaaa atggaatctg aaccctcagc
     1561 agttttatac catgttccct cataccccag acaacagctt tctggggttt gtggttgagc
     1621 agcacctgaa ctccagtgat atccaccaca ttaatgaaat caaaaggcag aaccagtccc
     1681 ttgtgtatgg caaagtggat agcttctgga agaataagaa gatctacttg gacattattc
     1741 acacatacat ggaagtgcat gcaactgttt atggctccag cacaaagaat attcccagtt
     1801 acgtgaaaaa ccatggtatc ctcagtggac gggacctgca gttccttctt cgagaaacca
     1861 agttgtttgt tggacttggg ttcccttacg agggcccagc tcccctggaa gctatcgcaa
     1921 atggatgtgc ttttctgaat cccaagttca acccacccaa aagcagcaaa aacacagact
     1981 ttttcattgg caagccaact ctgagagagc tgacatccca gcatccttac gctgaagttt
     2041 tcatcgggcg gccacatgtg tggactgttg acctcaacaa tcaggaggaa gtagaggatg
     2101 cagtgaaagc aattttaaat cagaagattg agccatacat gccatatgaa tttacgtgcg
     2161 aggggatgct acagagaatc aatgctttca ttgaaaaaca ggacttctgc catgggcaag
     2221 tgatgtggcc acccctcagc gccctacagg tcaagcttgc tgagcccggg cagtcctgca
     2281 agcaggtgtg ccaggagagc cagctcatct gcgagccttc tttcttccag cacctcaaca
     2341 aggacaagga catgctgaag tacaaggtga cctgccaaag ctcagagctg gccaaggaca
     2401 tcctggtgcc ctcctttgac cctaagaata agcactgtgt gtttcaaggt gacctcctgc
     2461 tcttcagctg tgcaggcgcc caccccaggc accagagggt ctgcccctgc cgggacttca
     2521 tcaagggcca ggtggctctc tgcaaagact gcctatagca gctacctgct cagccctgca
     2581 ccatgctgct ggggaagaca gtggccccag ccccgtcagg cagggccagg gacagaagtc
     2641 atgcagggac tctggcaaga gcctgaactt tttcgtagaa ggttctgaat tggcattgcc
     2701 cttgctgcac tccgagcaac ccagtggagt cttcaccaaa acaaaacaag agcgtatgtc
     2761 aggccaggag cctggcttgt ccctggcaca acatcatttc tgtttctcaa ggagcaactg
     2821 tgggaagact gtcactgcag ctgctccagg gcaaaagaaa gtctcaagag tcctttaaaa
     2881 caaaacagga ggaattgagc tgatgggaaa gaactctgaa tgggaatatt cctaaaccca
     2941 ttaacttatt tattcgggtg ggagggaggg gaccgcggga gggagaggag ggattgatca
     3001 caggcttctt ttatttccac tgttaatcat ccaccttcac tatactgttg ttctgtctgc
     3061 tttgggcagg gtgcaggaga gagaccgtgt gccgtggggc tggtggcctg ctcagggaca
     3121 gccatgggga cctggcagct caaaacagat ggcagcagaa caaaagaaac ttttgctttg
     3181 gaagcacagc caaactcccc agggcatcgt catagatggc acctagagca tgggctgcct
     3241 cagtcagggg gacgtgcctg tgttgtccag agagcccagc cagggacgaa aaaggagatg
     3301 aggccccttt cctccatgtc cccatgccca gacacatacc ttggccgtaa tttcttttag
     3361 aatccctttg ggaaggaaat acaggattga gggattttgg agtttctggt aaactcaccc
     3421 tccctccagc cccgctatga ggaggaaagt agagatgaaa gactcacgct gttcatggac
     3481 ttggagagga ttatctttga gccaagattt ggacaggagt gaagtcggtc cttaagaatt
     3541 tgtggcgcga ccggagctgg gctcctgaca ggtgggcttg tgttgtgtcc cctctgatgg
     3601 caccaaagtc cagggaaggg ggctcttgat gtctgctggg ggagtggggg cagctcagga
     3661 tgaatgcagt gccctgtcct ggctactcac ctgagggtgt agctcgcaaa ggtgggaatc
     3721 tggtgctggc ttttccttca ggcaggatca ctctgacttg ttgagtaatc agtcatcagg
     3781 ttggcctggt cagacacact ggaccccacc ttccaaaccc tggacccccc catgctctgc
     3841 ctttgtataa gttcccaccc cactgaatca ttgctgccat gctctgtaca agtttgtaag
     3901 tttctgaaaa gcccctgaca tctccatgct gatgcaagtg agaccccttc tgcctaatgt
     3961 gagcggttgg cgtcctccac ttggcctctg ctgggcgcct cagtcgctca atgatgtgct
     4021 ctgtgccggg gcttccaagc acccttcctg ctaacagagg gcggcccagt ggagcctccg
     4081 ctgggcagaa ctgatgggca aggcgtaatg gctggtagct ttcagaatgt gagggaaagg
     4141 aagaattccg cttcatgggg actagagtta gtgtggggcc tttaagtctg ggaagttaca
     4201 ttctgcttct ttctcaattg ctacacaaat gtgcagccag ccttttttct taggcccact
     4261 gagatccctg ctcagtgcgt ctcaattgta tctccagtct aagaggaggg tggggggcac
     4321 ccggggcctc ctccactcct taggtggccc ccaagcacat ctgccaggta gagtaccatg
     4381 gggaggcctc tgccctgagc atcatcacag gggcgcctcc agacctggct gagaatagcc
     4441 tctgcttggg cctaggaaga agggcagaag tccaaaggaa accttggtga gtgagtgtgt
     4501 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtatgagcct gtgcatttct
     4561 tttaagcaag ggcagtttcc tgcagtagga gggcagatga ctggcatcct tgctgtaagg
     4621 aagagctttt tccttgtgaa tgggcctctt ggctgtaccc tagggtgtgg gaatttgcta
     4681 gagttccctg acctgcaatc cctgggccag tgccgccctc tcctgggctc ttccaggagc
     4741 attgaacact gaggatgtca atgcaagtat ctgaccaaca gggggagcct gcaggccgag
     4801 caaagtttaa cccacttagc cactgctact taagcaagga agctgaaagg taaccttagc
     4861 cctgccttgt gttccaaaag ctgcaaggat catttgctgg ctgtccctaa gttcaaggcc
     4921 tccctacctt ccccttcttt tccaagtcac aacccgccct gcccgggcca gacagcccag
     4981 aagaacactt ttctgccatt taaaaatccc tctttcatcc cccccagtcc agtattgagt
     5041 ggaagtgcgc cggagttcca ctgacagggc agctgctctt ggagcaaaca agcccacact
     5101 ccagttgttt agggcattat ctgaagagtc agtgacccct gaatgtgtac tggctcccag
     5161 gcctcctcag acttggccca ggtctagtca gtgatcctgg agaggaaact ggggaggggg
     5221 cttccaactg ccctgcatgg aagaggagag gaaggtgccc ctgtgtgggc tggaagcctg
     5281 cggaggtttt gccattgctg atcttgatag aatcctaaac cgatttatag ctgataacag
     5341 ttccatgggg agagaaatcc taagttatga ttaatgtttt tccccgctat aatatcttgc
     5401 ctgttttttt ggattatttt ccccattgtg atcctaagct cttaaaaaac ttgagggaaa
     5461 acattcatct aatttactaa aagagaaagc tttccattga aaggtagata ctttgaggag
     5521 taaaaagact tctttgaatg ctggtaaaca ccgcatttat tttgtgtatg cagtttgatt
     5581 tgcacatgta taaatggaga tgcttttcat ttttgtttgg actgggtttg tgtcactgct
     5641 cattacagtt tgcttttttg tgtgtttgct gtgcgtttgg agatattagt cagtttcttt
     5701 agtgatattt gtttccttga tgtgcctttt cgtttttctt tggggttttt ggaatccgga
     5761 tgctgttgaa gggcaatagc agactcctcc agctaagaga caggacatgt tcttgagcca
     5821 ctgtagctgt tgaagctgga caccagacgc tccctataac ccccccgcca ggccatagcg
     5881 tgtatgcatg tgcacttcca cccacagagg agggtgtgaa gccttgagaa cctcaagaaa
     5941 gggctggatt ctgccatacc tttgggtcta ccttgggact gctggttgcc aacgtgtcaa
     6001 ccagcctgtg ttccctgcca cccacgcact tgctgaggtg tggctgaggc agaatcatgt
     6061 gaatgggtgc atccaaggag ttcagggccc tgcttggaga agaaatactt tagcatcatg
     6121 aaagggaaag aacgtgcacc ccttttttgt ttctttagtg aatgcaagat ttaataaaag
     6181 tgaataatga gcttcccctt tgggagtgga gcccagtgca gctcactgac agggttgaca
     6241 tcagtatgat gtgttggact gaaactgtat gtctgtaggt aggtgtgtgc cttttagggc
     6301 agaccacggt ggccacccca tttctccaag gtggtttacc tagcttgtgt atattagaca
     6361 ttgccaccct cacctctggc caaaaattct tgatttaaaa agaaaagtct attttgttaa
     6421 cgacaggctc tgttgtatgt gttactatcc caagcctgga ttattttatt tatttaaaag
     6481 tattttaatt tccatattgg ctttattcta atcccatcca tccctgtgga gctgcagagc
     6541 atcttcatgt gagtagacgg atggacataa atagattcat gctcatttag gaagctggga
     6601 gtttcgtgaa gctgagggtg agttcctgtg attcttgttc gcttcaacaa aaagtgggag
     6661 accaagtttt tatagcaaaa gaccaaatta gctgtagagt cttgaatgca gaaaaaaatt
     6721 accctagctt tcttagcact tagggttttg tgaggattca gtgtttagca cagtgcttgg
     6781 cacatagtaa gccctagtaa atgttaaata ttgttattag tgtttcgtaa aacttgagaa
     6841 atagagctga gctcattccc ttcctgttga ttcaaaaata atacctacat gaaaacatga
     6901 ttccaagttg attgaatgtt gtaggaatta ctggtttaga gtagcccagt tctcggccta
     6961 ccctgctggt tgggatctta ctgtattctt gaatgcactg gtttgaaaat atgccagact
     7021 tcagccccca aggaaacaag gctgcaagaa tttatgaact ccagctggaa aaggtaaagg
     7081 tgacctttgg ctagccacat actggacctt accccactga cgtctttcag aacattccaa
     7141 gggttttcct caaggaacat ttttgagcta gaaattaaaa tgggttctct ggcagactgc
     7201 accccttgag tcaaagttaa cagtattcct ttgaatgcaa taatagaggc ttttctgcgt
     7261 taagggagaa ggaatgacca attgaactta cacattcccc aggcaggtcc ctttgccggc
     7321 ccctacaggc tggggtggcc cctcctgtcc tcagggatca gactcccaga ctggttagtt
     7381 ctgcatgttt ccatcaaatt aaaggttatt ccctggccgc ctcctggaga aaaccaaccc
     7441 caccctgcca gctgggggca atggggcagg gattttggcc tctcagaaca gctcctagag
     7501 gctgctcatg actgaatgtt ttcccaaatc acctaaatat cggtttgctt tttgttttgg
     7561 gggagaggat ttagcctctt acttccctga tggattcaaa gttttatcta tctccttatc
     7621 tcctgccctg tcttggcaca actctggata gattgcaggt gtggaatttg ctggagtttg
     7681 gtgacttcgt caaattcctt tggattctgt tccgccaaat cagcagtctc gtcctgtgga
     7741 tgcagtgact ggaatttccc atctgcaaag catctctgta gcccagattt tgtggagcct
     7801 taagacactc cctcaatgcc accctgaccc cacggctgga gaaccctgtg cttatgtggt
     7861 gggcagggcc actgttgatg gaggatggcg gggacggggt ggtgctcaaa ggatctgtgg
     7921 tgctggaggt ctacacgcct ctcaggacag cgtgtcagga acctcagagc agcttcacat
     7981 ctgccaaagc tgagagggag cgaacttggg aagcattttg ctcattgtcc tacccaagta
     8041 ttaatagcat aatagttgat gccaaaggag atggtgacgt cccttccact gtagttgctg
     8101 tcacaacctt gacgtcttta agctaatggc cgtttgcatc tgtgtcttca aacagatcct
     8161 ggttacagcc attttgtgtg attcacttcg ggggttaagt aatgcaggat tctgcaaaca
     8221 aggtgtcgcc gtccaaatgt actgtcctgg catagagagc actgctttgt tttccactgt
     8281 tgtagagaaa actagggaga actttatttt tcaataaact tttcttgtgt ga
//
