LOCUS       NM_014275               2365 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens alpha-1,3-mannosyl-glycoprotein
            4-beta-N-acetylglucosaminyltransferase B (MGAT4B), transcript
            variant 1, mRNA.
ACCESSION   NM_014275
VERSION     NM_014275.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Song,W., Isaji,T., Nakano,M., Liang,C., Fukuda,T. and Gu,J.
  TITLE     O-GlcNAcylation regulates beta1,4-GlcNAc-branched N-glycan
            biosynthesis via the OGT/SLC35A3/GnT-IV axis
  JOURNAL   FASEB J 36 (2), e22149 (2022)
   PUBMED   34981577
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 2365)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2365)
  AUTHORS   Maszczak-Seneczko,D., Sosicka,P., Kaczmarek,B., Majkowski,M.,
            Luzarowski,M., Olczak,T. and Olczak,M.
  TITLE     UDP-galactose (SLC35A2) and UDP-N-acetylglucosamine (SLC35A3)
            Transporters Form Glycosylation-related Complexes with Mannoside
            Acetylglucosaminyltransferases (Mgats)
  JOURNAL   J Biol Chem 290 (25), 15475-15486 (2015)
   PUBMED   25944901
  REMARK    GeneRIF: UDP-galactose (SLC35A2) and UDP-N-acetylglucosamine
            (SLC35A3) Transporters Form Glycosylation-related Complexes with
            Mannoside Acetylglucosaminyltransferases (Mgats).
REFERENCE   5  (bases 1 to 2365)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   6  (bases 1 to 2365)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 2365)
  AUTHORS   Kudo,T., Nakagawa,H., Takahashi,M., Hamaguchi,J., Kamiyama,N.,
            Yokoo,H., Nakanishi,K., Nakagawa,T., Kamiyama,T., Deguchi,K.,
            Nishimura,S. and Todo,S.
  TITLE     N-glycan alterations are associated with drug resistance in human
            hepatocellular carcinoma
  JOURNAL   Mol Cancer 6, 32 (2007)
   PUBMED   17488527
  REMARK    GeneRIF: We investigated mRNA levels of glycosyltransferases,
            namely, N-acetylglucosaminyltransferase a (GnT)-IVb, and found that
            (GnT)-IVb expression was increased in HLE-cells Epirubicin
            resistant.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2365)
  AUTHORS   Oguri,S., Yoshida,A., Minowa,M.T. and Takeuchi,M.
  TITLE     Kinetic properties and substrate specificities of two recombinant
            human N-acetylglucosaminyltransferase-IV isozymes
  JOURNAL   Glycoconj J 23 (7-8), 473-480 (2006)
   PUBMED   17006639
  REMARK    GeneRIF: GnT-IVa is more active than GnT-IVb under physiological
            conditions and it primarily contributes to the biosynthesis of
            N-glycans.
REFERENCE   9  (bases 1 to 2365)
  AUTHORS   Ide,Y., Miyoshi,E., Nakagawa,T., Gu,J., Tanemura,M., Nishida,T.,
            Ito,T., Yamamoto,H., Kozutsumi,Y. and Taniguchi,N.
  TITLE     Aberrant expression of N-acetylglucosaminyltransferase-IVa and IVb
            (GnT-IVa and b) in pancreatic cancer
  JOURNAL   Biochem Biophys Res Commun 341 (2), 478-482 (2006)
   PUBMED   16434023
  REMARK    GeneRIF: analysis of expression of
            N-acetylglucosaminyltransferase-IVa and IVb (GnT-IVa and b) in
            pancreatic cancer
REFERENCE   10 (bases 1 to 2365)
  AUTHORS   Yoshida,A., Minowa,M.T., Takamatsu,S., Hara,T., Ikenaga,H. and
            Takeuchi,M.
  TITLE     A novel second isoenzyme of the human
            UDP-N-acetylglucosamine:alpha1,3-D-mannoside
            beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning,
            expression, and chromosomal assignment
  JOURNAL   Glycoconj J 15 (12), 1115-1123 (1998)
   PUBMED   10372966
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL537522.3, BC051835.1 and
            AY358984.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014275.4.
            
            Summary: This gene encodes a key glycosyltransferase that regulates
            the formation of tri- and multiantennary branching structures in
            the Golgi apparatus. The encoded protein, in addition to the
            related isoenzyme A, catalyzes the transfer of N-acetylglucosamine
            (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the
            Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of
            R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,
            4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a
            role in regulating the availability of serum glycoproteins,
            oncogenesis, and differentiation. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the shorter isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051835.1, SRR1163658.230580.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000292591.12/ ENSP00000292591.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AL537522.3         87-124
            39-456              BC051835.1         1-418
            457-2365            AY358984.1         224-2132
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q35.3"
     gene            1..2365
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /note="alpha-1,3-mannosyl-glycoprotein
                     4-beta-N-acetylglucosaminyltransferase B"
                     /db_xref="GeneID:11282"
                     /db_xref="HGNC:HGNC:7048"
                     /db_xref="MIM:604561"
     exon            1..380
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     CDS             284..1930
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /EC_number="2.4.1.145"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UDP-N-acetylglucosamine: alpha-1,3-D-mannoside
                     beta-1,4-N-acetylglucosaminyltransferase IV;
                     alpha-1,3-mannosyl-glycoprotein
                     beta-1,4-N-acetylglucosaminyltransferase; mannosyl
                     (alpha-1,3-)-glycoprotein
                     beta-1,4-N-acetylglucosaminyltransferase, isoenzyme B;
                     aminyltransferase; N-glycosyl-oligosaccharide-glycoprotein
                     N-acetylglucosaminyltransferase IVb;
                     UDP-N-acetylglucosamine: alpha-1,3-D-mannoside
                     beta-1,4-N-acetylglucosaminyltransferase IVb; glcNAc-T
                     IVb; mannosyl (alpha-1,3-)-glycoprotein
                     beta-1,4-N-acetylglucosaminyltransferase, isozyme B"
                     /codon_start=1
                     /product="alpha-1,3-mannosyl-glycoprotein
                     4-beta-N-acetylglucosaminyltransferase B isoform 1"
                     /protein_id="NP_055090.1"
                     /db_xref="CCDS:CCDS4448.1"
                     /db_xref="GeneID:11282"
                     /db_xref="HGNC:HGNC:7048"
                     /db_xref="MIM:604561"
                     /translation="MRLRNGTFLTLLLFCLCAFLSLSWYAALSGQKGDVVDVYQREFL
                     ALRDRLHAAEQESLKRSKELNLVLDEIKRAVSERQALRDGDGNRTWGRLTEDPRLKPW
                     NGSHRHVLHLPTVFHHLPHLLAKESSLQPAVRVGQGRTGVSVVMGIPSVRREVHSYLT
                     DTLHSLISELSPQEKEDSVIVVLIAETDSQYTSAVTENIKALFPTEIHSGLLEVISPS
                     PHFYPDFSRLRESFGDPKERVRWRTKQNLDYCFLMMYAQSKGIYYVQLEDDIVAKPNY
                     LSTMKNFALQQPSEDWMILEFSQLGFIGKMFKSLDLSLIVEFILMFYRDKPIDWLLDH
                     ILWVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQAL
                     RKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFF
                     FRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYK
                     GVAEGEVDPAFGPLEALRLSIQTDSPVWVILSEIFLKKAD"
     exon            381..566
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            567..707
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            708..841
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            842..888
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1002
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1078
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1193
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1324
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1432
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1626
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1705
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1793
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1906
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..2365
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2348..2353
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /note="hexamer: AATAAA"
     polyA_site      2365
                     /gene="MGAT4B"
                     /gene_synonym="GNT-IV; GNT-IVB"
                     /note="major polyA site"
ORIGIN      
        1 agtgtcccgc gcccctgata tctgcagtga gcctgatacc tgcctctgcc cttctgagcc
       61 tgttcctctt ccctgagtac agggcacaaa gcttgcgccc tgaggggcgg ccggcgcgct
      121 ccctggcccg gtccccgccc ggccccgggc cccccgcccc tccccgaccc ggggccgggg
      181 cccctgccgc cgccgccgcc gccttccgac ccctgcgccc cggccccggt cccccgggcc
      241 atgcagcctc ggccccgcgg gcgcccgccg cgcacccgag gagatgaggc tccgcaatgg
      301 caccttcctg acgctgctgc tcttctgcct gtgcgccttc ctctcgctgt cctggtacgc
      361 ggcactcagc ggccagaaag gcgacgttgt ggacgtttac cagcgggagt tcctggcgct
      421 gcgcgatcgg ttgcacgcag ctgagcagga gagcctcaag cgctccaagg agctcaacct
      481 ggtgctggac gagatcaaga gggccgtgtc agaaaggcag gcgctgcgag acggagacgg
      541 caatcgcacc tggggccgcc taacagagga cccccgattg aagccgtgga acggctcaca
      601 ccggcacgtg ctgcacctgc ccaccgtctt ccatcacctg ccacacctgc tggccaagga
      661 gagcagtctg cagcccgcgg tgcgcgtggg ccagggccgc accggagtgt cggtggtgat
      721 gggcatcccg agcgtgcggc gcgaggtgca ctcgtacctg actgacactc tgcactcgct
      781 catctccgag ctgagcccgc aggagaagga ggactcggtc atcgtggtgc tgatcgccga
      841 gactgactca cagtacactt cggcagtgac agagaacatc aaggccttgt tccccacgga
      901 gatccattct gggctcctgg aggtcatctc accctccccc cacttctacc ctgacttctc
      961 ccgcctccga gagtcctttg gggaccccaa ggagagagtc aggtggagga ccaaacagaa
     1021 cctcgattac tgcttcctca tgatgtacgc gcagtccaaa ggcatctact acgtgcagct
     1081 ggaggatgac atcgtggcca agcccaacta cctgagcacc atgaagaact ttgcactgca
     1141 gcagccttca gaggactgga tgatcctgga gttctcccag ctgggcttca ttggtaagat
     1201 gttcaagtcg ctggacctga gcctgattgt agagttcatt ctcatgttct accgggacaa
     1261 gcccatcgac tggctcctgg accatattct gtgggtgaaa gtctgcaacc ccgagaagga
     1321 tgcgaagcac tgtgaccggc agaaagccaa cctgcggatc cgcttcaaac cgtccctctt
     1381 ccagcacgtg ggcactcact cctcgctggc tggcaagatc cagaaactga aggacaaaga
     1441 ctttggaaag caggcgctgc ggaaggagca tgtgaacccg ccagcagagg tgagcacgag
     1501 cctgaagaca taccagcact tcaccctgga gaaagcctac ctgcgcgagg acttcttctg
     1561 ggccttcacc cctgccgcgg gggacttcat ccgcttccgc ttcttccaac ctctaagact
     1621 ggagcggttc ttcttccgca gtgggaacat cgagcacccg gaggacaagc tcttcaacac
     1681 gtctgtggag gtgctgccct tcgacaaccc tcagtcagac aaggaggccc tgcaggaggg
     1741 ccgcaccgcc accctccggt accctcggag ccccgacggc tacctccaga tcggctcctt
     1801 ctacaaggga gtggcagagg gagaggtgga cccagccttc ggccctctgg aagcactgcg
     1861 cctctcgatc cagacggact cccctgtgtg ggtgattctg agcgagatct tcctgaaaaa
     1921 ggccgactaa gctgcgggct tctgagggta ccctgtggcc agccctgaag cccacatttc
     1981 tgggggtgtc gtcactgccg tccccggagg gccagatacg gccccgccca aagggttctg
     2041 cctggcgtcg ggcttgggcc ggcctggggt ccgccgctgg cccggaggcc ctaggagctg
     2101 gtgctgcccc cgcccgccgg gccgcggagg aggcaggcgg cccccacact gtgcctgagg
     2161 cccggaaccg ttcgcacccg gcctgcccca gtcaggccgt tttagaagag cttttacttg
     2221 ggcgcccgcc gtctctggcg cgaacactgg aatgcatata ctactttatg tgctgtgttt
     2281 tttattcttg gatacatttg attttttcac gtaagtccac atatacttct ataagagcgt
     2341 gacttgtaat aaagggttaa tgaag
//
