LOCUS       NM_012214               8388 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens alpha-1,3-mannosyl-glycoprotein
            4-beta-N-acetylglucosaminyltransferase A (MGAT4A), transcript
            variant 1, mRNA.
ACCESSION   NM_012214
VERSION     NM_012214.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Yang,D., Han,F., Cai,J., Sun,H., Wang,F., Jiang,M., Zhang,M.,
            Yuan,M., Zhou,W., Li,H., Yang,L., Bai,Y., Xiao,L., Dong,H.,
            Cheng,Q., Mao,H., Zhou,L., Wang,R., Li,Y. and Nie,H.
  TITLE     N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the
            interaction of integrin beta1 with vimentin and promotes
            hepatocellular carcinoma cell motility
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1870 (7), 119513 (2023)
   PUBMED   37295747
  REMARK    GeneRIF: N-glycosylation by N-acetylglucosaminyltransferase IVa
            enhances the interaction of integrin beta1 with vimentin and
            promotes hepatocellular carcinoma cell motility.
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Nishino,K., Yamamoto,E., Niimi,K., Sekiya,Y., Yamashita,Y. and
            Kikkawa,F.
  TITLE     N-acetylglucosaminyltransferase IVa promotes invasion of
            choriocarcinoma
  JOURNAL   Oncol Rep 38 (1), 440-448 (2017)
   PUBMED   28534963
  REMARK    GeneRIF: GnT-IVa may contribute to the malignancy of
            choriocarcinoma by promoting cell adhesion, migration and invasion
            through glycosylation of integrin beta1 and LAMP-2.
REFERENCE   3  (bases 1 to 8388)
  AUTHORS   Vaiana,C.A., Kurcon,T. and Mahal,L.K.
  TITLE     MicroRNA-424 Predicts a Role for beta-1,4 Branched Glycosylation in
            Cell Cycle Progression
  JOURNAL   J Biol Chem 291 (3), 1529-1537 (2016)
   PUBMED   26589799
  REMARK    GeneRIF: Data suggest microRNA-424 regulates expression of MGAT4A
            (mannoside beta-1,4-N-acetylglucosaminyltransferase A), OGT
            (O-linked N-acetylglucosamine transferase), and GALNT13
            (polypeptide N-acetylgalactosaminyltransferase 13) in mammary
            epithelium.
REFERENCE   4  (bases 1 to 8388)
  AUTHORS   Niimi,K., Yamamoto,E., Fujiwara,S., Shinjo,K., Kotani,T., Umezu,T.,
            Kajiyama,H., Shibata,K., Ino,K. and Kikkawa,F.
  TITLE     High expression of N-acetylglucosaminyltransferase IVa promotes
            invasion of choriocarcinoma
  JOURNAL   Br J Cancer 107 (12), 1969-1977 (2012)
   PUBMED   23169300
  REMARK    GeneRIF: Findings suggest that GnT-IVa is involved in regulating
            invasion of choriocarcinoma through modifications of the
            oligosaccharide chains of beta1 integrin.
REFERENCE   5  (bases 1 to 8388)
  CONSRTM   Psychiatric GWAS Consortium Bipolar Disorder Working Group
  TITLE     Large-scale genome-wide association analysis of bipolar disorder
            identifies a new susceptibility locus near ODZ4
  JOURNAL   Nat Genet 43 (10), 977-983 (2011)
   PUBMED   21926972
  REMARK    Erratum:[Nat Genet. 2012 Sep;44(9):1072. Fullerton, Janice M
            [added]; Hyoun, Phil L [corrected to Lee, Phil H]; Meng, Fan Guo
            [corrected to Meng, Fan]]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8388)
  AUTHORS   Kudo,T., Nakagawa,H., Takahashi,M., Hamaguchi,J., Kamiyama,N.,
            Yokoo,H., Nakanishi,K., Nakagawa,T., Kamiyama,T., Deguchi,K.,
            Nishimura,S. and Todo,S.
  TITLE     N-glycan alterations are associated with drug resistance in human
            hepatocellular carcinoma
  JOURNAL   Mol Cancer 6, 32 (2007)
   PUBMED   17488527
  REMARK    GeneRIF: We investigated mRNA levels of glycosyltransferases,
            namely, N-acetylglucosaminyltransferase a (GnT)-IVa, and found that
            (GnT)-IVa expression was decreased in HLE-cells Epirubicin
            resistant.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 8388)
  AUTHORS   Oguri,S., Yoshida,A., Minowa,M.T. and Takeuchi,M.
  TITLE     Kinetic properties and substrate specificities of two recombinant
            human N-acetylglucosaminyltransferase-IV isozymes
  JOURNAL   Glycoconj J 23 (7-8), 473-480 (2006)
   PUBMED   17006639
  REMARK    GeneRIF: GnT-IVa is more active than GnT-IVb under physiological
            conditions and it primarily contributes to the biosynthesis of
            N-glycans.
REFERENCE   8  (bases 1 to 8388)
  AUTHORS   Ide,Y., Miyoshi,E., Nakagawa,T., Gu,J., Tanemura,M., Nishida,T.,
            Ito,T., Yamamoto,H., Kozutsumi,Y. and Taniguchi,N.
  TITLE     Aberrant expression of N-acetylglucosaminyltransferase-IVa and IVb
            (GnT-IVa and b) in pancreatic cancer
  JOURNAL   Biochem Biophys Res Commun 341 (2), 478-482 (2006)
   PUBMED   16434023
  REMARK    GeneRIF: analysis of expression of
            N-acetylglucosaminyltransferase-IVa and IVb (GnT-IVa and b) in
            pancreatic cancer
REFERENCE   9  (bases 1 to 8388)
  AUTHORS   D'Arrigo,A., Belluco,C., Ambrosi,A., Digito,M., Esposito,G.,
            Bertola,A., Fabris,M., Nofrate,V., Mammano,E., Leon,A., Nitti,D.
            and Lise,M.
  TITLE     Metastatic transcriptional pattern revealed by gene expression
            profiling in primary colorectal carcinoma
  JOURNAL   Int J Cancer 115 (2), 256-262 (2005)
   PUBMED   15688387
  REMARK    GeneRIF: upregulation of mannosyl (alpha-1,3-)-glycoprotein
            beta-1,4-N-acetyl-glucosaminyl-transferase is associated with
            metastatic colorectal carcinoma
REFERENCE   10 (bases 1 to 8388)
  AUTHORS   Yoshida,A., Minowa,M.T., Takamatsu,S., Hara,T., Oguri,S.,
            Ikenaga,H. and Takeuchi,M.
  TITLE     Tissue specific expression and chromosomal mapping of a human
            UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1,
            4-N-acetylglucosaminyltransferase
  JOURNAL   Glycobiology 9 (3), 303-310 (1999)
   PUBMED   10024668
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC109826.4, AC064860.6 and
            AB000616.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012214.2.
            
            Summary: This gene encodes a key glycosyltransferase that regulates
            the formation of tri- and multiantennary branching structures in
            the Golgi apparatus. The encoded protein, in addition to the
            related isoenzyme B, catalyzes the transfer of N-acetylglucosamine
            (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the
            Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of
            R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,
            4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a
            role in regulating the availability of serum glycoproteins,
            oncogenesis, and differentiation. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.83449.1,
                                           SRR1660805.233529.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393487.6/ ENSP00000377127.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                AC109826.4         23985-24069         c
            86-414              AC109826.4         19176-19504         c
            415-582             AC064860.6         64134-64301         c
            583-723             AC064860.6         60865-61005         c
            724-857             AC064860.6         48876-49009         c
            858-904             AC064860.6         44048-44094         c
            905-1018            AC064860.6         42182-42295         c
            1019-1094           AC064860.6         41275-41350         c
            1095-1209           AC064860.6         31258-31372         c
            1210-1340           AC064860.6         29753-29883         c
            1341-1448           AC064860.6         25951-26058         c
            1449-1642           AC064860.6         25638-25831         c
            1643-1721           AC064860.6         22347-22425         c
            1722-1788           AC064860.6         21052-21118         c
            1789-1901           AC064860.6         11553-11665         c
            1902-2300           AB000616.1         1717-2115
            2301-8388           AC064860.6         4936-11023          c
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q11.2"
     gene            1..8388
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /note="alpha-1,3-mannosyl-glycoprotein
                     4-beta-N-acetylglucosaminyltransferase A"
                     /db_xref="GeneID:11320"
                     /db_xref="HGNC:HGNC:7047"
                     /db_xref="MIM:604623"
     exon            1..85
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            86..414
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     CDS             321..1928
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /EC_number="2.4.1.145"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UDP-N-acetylglucosamine:alpha1,3-d-mannoside
                     beta1,4-N-acetylglucosaminyltransferase;
                     alpha-1,3-mannosyl-glycoprotein
                     beta-1,4-N-acetylglucosaminyltransferase; mannosyl
                     (alpha-1,3-)-glycoprotein
                     beta-1,4-N-acetylglucosaminyltransferase, isoenzyme A;
                     mannosyl (alpha-1,3-)-glycoprotein
                     beta-1,4-N-acetylglucosaminyltransferase, isozyme A;
                     UDP-GlcNAc:a-1,3-D-mannoside
                     b-1,4-acetylglucosaminyltransferase IV;
                     N-acetylglucosaminyltransferase IVa;
                     N-glycosyl-oligosaccharide-glycoprotein
                     N-acetylglucosaminyltransferase IVa;
                     UDP-N-acetylglucosamine: alpha-1,3-D-mannoside
                     beta-1,4-N-acetylglucosaminyltransferase IVa; glcNAc-T
                     IVa"
                     /codon_start=1
                     /product="alpha-1,3-mannosyl-glycoprotein
                     4-beta-N-acetylglucosaminyltransferase A isoform 1"
                     /protein_id="NP_036346.1"
                     /db_xref="CCDS:CCDS2036.1"
                     /db_xref="GeneID:11320"
                     /db_xref="HGNC:HGNC:7047"
                     /db_xref="MIM:604623"
                     /translation="MRLRNGTVATALAFITSFLTLSWYTTWQNGKEKLIAYQREFLAL
                     KERLRIAEHRISQRSSELNTIVQQFKRVGAETNGSKDALNKFSDNTLKLLKELTSKKS
                     LQVPSIYYHLPHLLKNEGSLQPAVQIGNGRTGVSIVMGIPTVKREVKSYLIETLHSLI
                     DNLYPEEKLDCVIVVFIGETDIDYVHGVVANLEKEFSKEISSGLVEVISPPESYYPDL
                     TNLKETFGDSKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNF
                     ALQLSSEEWMILEFSQLGFIGKMFQAPDLTLIVEFIFMFYKEKPIDWLLDHILWVKVC
                     NPEKDAKHCDRQKANLRIRFRPSLFQHVGLHSSLSGKIQKLTDKDYMKPLLLKIHVNP
                     PAEVSTSLKVYQGHTLEKTYMGEDFFWAITPIAGDYILFKFDKPVNVESYLFHSGNQE
                     HPGDILLNTTVEVLPFKSEGLEISKETKDKRLEDGYFRIGKFENGVAEGMVDPSLNPI
                     SAFRLSVIQNSAVWAILNEIHIKKATN"
     exon            415..582
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            583..723
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            724..857
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            858..904
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1018
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1094
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1209
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1340
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1448
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1642
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1721
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1788
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..1901
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..8388
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8323..8328
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /note="hexamer: AATAAA"
     polyA_site      8344
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /note="major polyA site"
     regulatory      8367..8372
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /note="hexamer: AATAAA"
     polyA_site      8388
                     /gene="MGAT4A"
                     /gene_synonym="GnT-4a; GNT-IV; GNT-IVA"
                     /note="major polyA site"
ORIGIN      
        1 gcggcccgca gcggcggcgg cagcggcggc ggcggctaca gcagctccgc cggcagccgc
       61 gggagcacgg cgacgccagc ggcgggaagg gaaaaggccg aggcatcagc gtgtgaagac
      121 cgcaaagacg atcccgagta cagttgtgaa cagcattgct gctaggctcc tcctgcagat
      181 catctgaaat gaacctctct tattgatttt tattggccta gagccaggag tactgcattc
      241 agttgacttt cagggtaaaa agaaaacagt cctggttgtt gtcatcataa acatatggac
      301 cagtgtgatg gtgaaatgag atgaggctcc gcaatggaac tgtagccact gctttagcat
      361 ttatcacttc cttccttact ttgtcttggt atactacatg gcaaaatggg aaagaaaaac
      421 tgattgctta tcaacgagaa ttccttgctt tgaaagaacg tcttcgaata gctgaacaca
      481 gaatctcaca gcgctcttct gaattaaata cgattgtgca acagttcaag cgtgtaggag
      541 cagaaacaaa tggaagtaag gatgcgttga ataagttttc agataatacc ctaaagctgt
      601 taaaggagtt aacaagcaaa aaatctcttc aagtgccaag tatttattat catttgcctc
      661 atttattgaa aaatgaagga agtcttcaac ctgctgtaca gattggcaac ggaagaacag
      721 gagtttcaat agtcatgggc attcccacag tgaagagaga agttaaatct tacctcatag
      781 aaactcttca ttcccttatt gataacctgt atcctgaaga gaagttggac tgtgttatag
      841 tagtcttcat aggagagaca gatattgatt atgtacatgg tgttgtagcc aacctggaga
      901 aagaattttc taaagaaatc agttctggct tggtggaagt catatcaccc cctgaaagct
      961 attatcctga cttgacaaac ctaaaggaga catttggaga ctccaaagaa agagtaagat
     1021 ggagaacaaa gcaaaaccta gattactgtt ttctaatgat gtatgctcaa gaaaagggca
     1081 tatattacat tcagcttgaa gatgatatta ttgtcaaaca aaattatttt aataccataa
     1141 aaaattttgc acttcaactt tcttctgagg aatggatgat tctagagttt tcccagctgg
     1201 gcttcattgg taaaatgttt caagcgccgg atcttactct gattgtagaa ttcatattca
     1261 tgttttacaa ggagaaaccc attgattggc tcctggacca tattctctgg gtgaaagtct
     1321 gcaaccctga aaaagatgca aaacattgtg atagacagaa agcaaatctg cgaattcgct
     1381 tcagaccttc ccttttccaa catgttggtc tgcactcatc actatcagga aaaatccaaa
     1441 aactcacgga taaagattat atgaaaccat tacttcttaa aatccatgta aacccacctg
     1501 cggaggtatc tacttccttg aaggtctacc aagggcatac gctggagaaa acttacatgg
     1561 gagaggattt cttctgggct atcacaccga tagctggaga ctacatcttg tttaaatttg
     1621 ataaaccagt caatgtagaa agttatttgt tccatagcgg caaccaagaa catcctggag
     1681 atattctgct aaacacaact gtggaagttt tgccttttaa gagtgaaggt ttggaaataa
     1741 gcaaagaaac caaagacaaa cgattagaag atggctattt cagaatagga aaatttgaga
     1801 atggtgttgc agaaggaatg gtggatccaa gtctcaatcc catttcagcc tttcgacttt
     1861 cagttattca gaattctgct gtttgggcca ttcttaatga gattcatatt aaaaaagcca
     1921 ccaactgatc atctgagaaa ccaacacatt ttttcctgtg aatttgttaa ttaaagatag
     1981 ttaagcatgt atcttttttt tatttctact tgaacactac ctcttgtgaa gtctactgta
     2041 gataagacga ttgtcatttc cacttggaaa gtgaatctcc cataataatt gtatttgttt
     2101 gaaactaagc tgtcctcaga ttttaacttg actcaaacat ttttcaatta tgacagcctg
     2161 ttaatatgac ttgtactatt ttggtattat actaatacat aagagttgta catattgtta
     2221 cattctttaa atttgagaaa aactaatgtt acatacattt tatgaagggg gtacttttga
     2281 ggttcactta ttttactatt atagaccctc ttttatagat tatcagggat tatatatata
     2341 aatatataaa tatacataaa aatgttatgg aattaattta ttagaaacac ttaagaagta
     2401 catatttttg tgcagtagat ttttgaatcg atactttttt tgaaaaccag tttccttgct
     2461 tttttaagtt ctttctatat ttttctttgg aaatgcaaac attacaaacc aatgccattt
     2521 ttcaaatgca ctgccattta agattgatta tagatggatt tcttaattga agtactttta
     2581 taatcacagt gactgaacaa aatattttca aagacatttg tcattcctta aagccaagat
     2641 tttaaagact aatgtccttc ctgagggtta ctttactata ctgtgtatgg tgtatagcca
     2701 cagaaagtca gtctgataaa ttttcaatgt gtaagtgtga tgcattcaac ccagattatt
     2761 gtagacttaa cttattatat ttcctctgat atgtaaaatg acaaactctt ctttcctttt
     2821 taatctttgt ctttaatcca tgaattaggt gcattatctg ctgttgattc ttttatatct
     2881 gcactcattt tatgacgtca gacaaaatgt tttctaggtg ttgctattgt atgagtcata
     2941 attggtataa ttttaaagaa aattgtaata ttgttttttg gtgaaatata ttattagtag
     3001 tatattttcc aaaatgattt tattgtagta tgaactgata agtgagtccc gtgtccaaga
     3061 gactgtattt attactctta aacacgctat tccaaatgat attactacta taaacaaaga
     3121 atattaccgg tctcagcaat cagtgatctt tataaaagta cttttaaaaa atgatgaatt
     3181 atcaccaggc gctgtggctc acgcctgtaa tcccagcact ttgggaggcc gaggcgggcg
     3241 gatcacgagg tcaggagatc aagaccatcc tggctaacac ggtgaaaccc tgtctctact
     3301 aaaaatacaa aaaattagcc aggcgtggtg gcaggtgcct gtaatcccag ctacttggga
     3361 ggctgaggca ggagaatggc gtgaacccag gaggcagagc tggcagtgag ctgagatcgc
     3421 accactgcac tccagcctgg gcgacacagc tagactccgt ctcaaaaaaa aaaaaagatg
     3481 aagtatcaac atcagttccc agccagtctg cttggacaca ctggggtgcc acaagtgggt
     3541 tgcaggttgg ttgcagacgg gcagagatgg attctgctga gctttcagag tgaccactgg
     3601 ctggggccta gcgctggggc aaggttccag ctggctgcct ctagcctgga ggagcttcat
     3661 ctagttaccc aatgtgctga accaacagca tgaaatagca tcatttcctg tgctgtgtta
     3721 caaagtgcaa acgtttggaa acactgatac agtacaatta tttccatcac tccatgttgt
     3781 tataattttt agttactttt cttgattgaa aagtcaatca cattgccaaa aatgattttt
     3841 atgtcagtta aaggccagtt gaaatttttt tattaggatg ttttaaattt acttttcact
     3901 ttcttgaatt tttttcttct caaatttcct tggcccagtg cccatgagca aatttcattc
     3961 aaaaacagga ataacctttt gtcataaaat tttggcacca gcttggtata ctaaattact
     4021 gcaaagataa ctgagtacca gaagtaaaaa tctctgcagg tgggcttagg agtactcttg
     4081 ggtccctggt tcctcagttg ccaactctgg tttcatgaag aaagaccatg ctctgaaaat
     4141 ctagggagga gaaagttatt gcagcacctg gtgtgatttg ttacaacttc tttaaagaca
     4201 gcctgcctgg ctagcttttt cctgttttca tgcagtttgc cctcccagca ttggtccacc
     4261 tgttgttccc aagaaactca ttcttgttcc caagactcct tttagccaaa tctttcctat
     4321 cattccagga cttttttgtg cttctctccc catgaatcta cccgtgccta gggtgtgtgc
     4381 caggcacgtg gcaggtggcc agtcagcatc tggatgtgag accaattatt ccagcctgcc
     4441 ctcctctttc tctttttatc attttgtatt atttttgaga cagggtctcg ctttgttgcc
     4501 caggctggag tgcagtggtg ctctcatagc tcactgcagc ctcgatctct ggcctcaagc
     4561 aatcctcctg cctcagcctc ccaaagtgct gggattatag gtgtgtgcca ccatgtccag
     4621 ccccctttct ctctcttaac tccctctccc tgctgtcgtt gccatactct tgcatacaca
     4681 atcggttgtc tagtttttga ttgtttgtgt gtatggtgtg tgctgtctct ctgaccagat
     4741 ttcaggttcc tgaggcgagc ctgcagctca tactgctcat ctgtcctctc ctatggtggg
     4801 tgctcagggc ctctcactgt tagttactcc ctcctttctg cccagttctg cactcaacta
     4861 gtagaagcag ccatcctttc cccaagcagg aaattgtagt ggtcgccctt aagagcagtg
     4921 tgagggcaga agattaaggg aggggaagag tccctggaac tggaagaagg taaatacttt
     4981 gccttgagag ggcgccgaat cattttacca aaatagtaaa tggaaaaagt gtcaaaggtt
     5041 gggactagtt ttaaaaacac atatagccag tagacacatg ggggctgttt aaataaaaac
     5101 atttttttac ccggttctgc catcacacac actagtgaca ttccaagtac tcagtggcca
     5161 tgtggagcta gtggccacca cacttgacac cacggatgca gaacttttcc atcactgcag
     5221 aaagctctgt tgaacagtac tgtgtccaga gtgctaatta tgaacagctt agaaaccaca
     5281 attaatacat tctcatgagg ttaaaagcag acatgggaat agaacataat gcaaatgatg
     5341 atgaatattt tgggtgagaa gagaaagctg aggacccatt tctaggattc taagatgtaa
     5401 gatttcttaa gttctttatc ttagtctcat gcattctcca catcacgcgc tgtaccatac
     5461 tgtgtagtca gaacagacag tgtgattgaa aagctttgga aaaagttaac acaaaggatt
     5521 atttagcaca taggctgtag atacgtatgt gtgtatttgt tcaacaattg gagatggttg
     5581 aatacccttg aacaaagtgt gtatcttctc aaatcagtgg ttgcactagt caataattag
     5641 aaggtgttgt tatttttaaa actataagca aaattatgaa ggcctttaaa aaatctatca
     5701 taataatgaa aaagaggttg tctcccaaca gtgctgtccc tcaaagaaaa gactggttat
     5761 gtggaaacag cacgtttgga gagattattc tagtgaataa cagtgtatat ttgtggtagg
     5821 caaatttcta agaatgaccc ctactgacac ccccaccaat gaccttgtat aatatcctcc
     5881 cactttgagc atgggtggaa cctgtttgat ccatgattat atcatgttat gtagcaaaag
     5941 gaagactgtc tgggggtggc taatcaaatc ttgtgactcc tttaaaagca gagagttttc
     6001 tcgggctggt ggcagaagtc aagagacaaa gcagaaggac ctcaggcacc attgctgttt
     6061 taaggtagag ggggctgcat gagaaggaat gcacatggct ttgaggagct gagagaggcc
     6121 ttggctaaca gcaaaggagc tgggaactgc agtcctacag ccacgggaac tgaattcagc
     6181 ccataagctc attcataggc ttggaaaggc attcatctcc agtctccaga taagacccag
     6241 cctggccaac actttgaatc cagccttgtg agaccctaag ccgagaactc acttaagcct
     6301 gcttggactt ctgacctaca gaactatgag ataataaatc agtggtgttt taagctgcta
     6361 aatttgtggc aatttgttat gtaggaatag gaaatgaata cagtatttta aaagaggtaa
     6421 agaggaaatc ttcatacttg attccctgta gtatgctgga tatttcttgg taaatgcatg
     6481 aataaagtct ttagtagcaa gtcatgtacc tggaccttgt tggggatgtt ctcgccagag
     6541 atctcaatta gtcttcattg gacgtttgtt ggcaaagttg cagcatctta ctgagtagcg
     6601 tttggtaaag gaactgctag aatgagacta taggaatagg actgaaacag tgtgtaaggg
     6661 atctttttta tttctagaag aggtgttact ttatactgat ttagactgct ctgagcctgt
     6721 cagaagtaaa ttcaagaatg attaacagtg gtattttaga tacattaaga gcaaaaataa
     6781 cctttgcctt gtagagatca gataatctgt gtatcgaggg atttaatttt ttttaaccaa
     6841 aacttagttt ctctttttat catctagcac agatcaaatc ctgtatgggc cagccaaatg
     6901 tttgtagttt gagaatgttt tgcaaatacc tctctgcttc caagccatta atagctaatg
     6961 aagttttatt ttggtttgtc cattaatttt tctgcttatt tttactgtat tgccatgtcc
     7021 ttgatacgag acaagtagaa ctaaaagaga gaaaatgaga ggttaagact gtcagaagat
     7081 gagaaatgtg agtcataacc ataccttagt gttttaaggg aactttatcc agtccaatcc
     7141 ctttatagaa aaggaacttt tccaagttat gttgcagtat ttatagtgtg taaaattttg
     7201 tttgactttg gtagttgtgc attttaaatt atgaatctta tatgctacga taactagtat
     7261 gcttactttc tttaatgcat gtgatcatac aatgctaaaa aggtattttt taaagaccca
     7321 accaaactga ggattttgtt atacatatct gtatcaacac ctatatataa actcttcttc
     7381 ttacctgaaa cactccttca cccggtttgg gcactaacta gatcattttc aaaggaacaa
     7441 acaggaagga gcaagctggc actccagggt gcatctgagc tgagaattta gaagacctca
     7501 ttcaagaaag taaactgagc attgatggga aagcactttg cgcgaagtaa cattcccact
     7561 gttaatgttg cagtcaaaat tgtggaaaaa aaaaagtggt gctaaaattg ttctgaaatg
     7621 aagattgttt gttgtgggga atattttaat actcttggac tttagggagg ctattttatg
     7681 tgtcacttgc tcctagttgt ttcattgtat tagtaattag caaactgtcc tggaaccaag
     7741 ggtttggtac ttttgcctga ttagcattcc attatttatt tgaaatgctc agtgtttgct
     7801 caaatttggt gaaaatatct tttgaagaca tattctctga gccttaaaaa tggctgctat
     7861 cgacagtact gtaagatgca ctgccagtag atttgatact tttcatttca gtgtaggcag
     7921 taatgttttc taagaaagat ttagaaatca gcaaagtgta acaattgtgt ttcatttatg
     7981 gaatggaaaa actgaagtgt cccattcaga acactttgga aaggttgtct gcattagggg
     8041 atgtttctgg aagcatccct tagagaagct gcctgaggaa tctccttgcc aggttgtacc
     8101 agattttttt ttttcttaaa tgtattgtat atattttcct taaaatgtct tcattggctg
     8161 aatgattcct atccgtagta atattttagg tacttgttgg atttttaaag gaagattatt
     8221 tattttcaga tttgctgcta taattgaaaa cctagtaact ggtcctgttg attgtgcctt
     8281 catcactgtt tctctgtgcc acgaccgttc atgtgtattt gaaataaaga agtttaaaaa
     8341 gccatgttga attcaagatt ccttctaata aaaataggtg aaacttaa
//
