LOCUS       NM_002406               8445 bp    mRNA    linear   PRI 05-AUG-2024
DEFINITION  Homo sapiens alpha-1,3-mannosyl-glycoprotein
            2-beta-N-acetylglucosaminyltransferase (MGAT1), transcript variant
            2, mRNA.
ACCESSION   NM_002406
VERSION     NM_002406.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Li,Y., Chen,Y., Wang,D., Wu,L., Li,T., An,N. and Yang,H.
  TITLE     Elucidating the multifaceted role of MGAT1 in hepatocellular
            carcinoma: integrative single-cell and spatial transcriptomics
            reveal novel therapeutic insights
  JOURNAL   Front Immunol 15, 1442722 (2024)
   PUBMED   39081317
  REMARK    GeneRIF: Elucidating the multifaceted role of MGAT1 in
            hepatocellular carcinoma: integrative single-cell and spatial
            transcriptomics reveal novel therapeutic insights.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8445)
  AUTHORS   Bi,W., Yang,M., Xing,P. and Huang,T.
  TITLE     MicroRNA miR-331-3p suppresses osteosarcoma progression via the
            Bcl-2/Bax and Wnt/beta-Catenin signaling pathways and the
            epithelial-mesenchymal transition by targeting
            N-acetylglucosaminyltransferase I (MGAT1)
  JOURNAL   Bioengineered 13 (6), 14159-14174 (2022)
   PUBMED   35758024
  REMARK    GeneRIF: MicroRNA miR-331-3p suppresses osteosarcoma progression
            via the Bcl-2/Bax and Wnt/beta-Catenin signaling pathways and the
            epithelial-mesenchymal transition by targeting
            N-acetylglucosaminyltransferase I (MGAT1).
REFERENCE   3  (bases 1 to 8445)
  AUTHORS   Akintayo,A., Mayoral,J., Asada,M., Tang,J., Sundaram,S. and
            Stanley,P.
  TITLE     Point mutations that inactivate MGAT4D-L, an inhibitor of MGAT1 and
            complex N-glycan synthesis
  JOURNAL   J Biol Chem 295 (41), 14053-14064 (2020)
   PUBMED   32763972
  REMARK    GeneRIF: Point mutations that inactivate MGAT4D-L, an inhibitor of
            MGAT1 and complex N-glycan synthesis.
REFERENCE   4  (bases 1 to 8445)
  AUTHORS   Li,Y., Liu,Y., Zhu,H., Chen,X., Tian,M., Wei,Y., Gong,Y. and
            Jiang,J.
  TITLE     N-acetylglucosaminyltransferase I promotes glioma cell
            proliferation and migration through increasing the stability of the
            glucose transporter GLUT1
  JOURNAL   FEBS Lett 594 (2), 358-366 (2020)
   PUBMED   31494931
  REMARK    GeneRIF: MGAT1 promotes complex N-glycosylation of glucose
            transporter 1 (Glut1) and increases Glut1 protein levels. In
            summary, our findings indicate that MGAT1 is highly expressed in
            glioblastoma and promotes glioma cells at least partly through
            upregulation of Glut1 protein.
REFERENCE   5  (bases 1 to 8445)
  AUTHORS   Akiva,I. and Birgul Iyison,N.
  TITLE     Identification of IFITM3 and MGAT1 as novel interaction partners of
            BRI3 by yeast two-hybrid screening
  JOURNAL   Turk J Biol 42 (6), 463-470 (2018)
   PUBMED   30983867
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8445)
  AUTHORS   Yip,B., Chen,S.H., Mulder,H., Hoppener,J.W. and Schachter,H.
  TITLE     Organization of the human beta-1,2-N-acetylglucosaminyltransferase
            I gene (MGAT1), which controls complex and hybrid N-glycan
            synthesis
  JOURNAL   Biochem J 321 (Pt 2) (Pt 2), 465-474 (1997)
   PUBMED   9020882
REFERENCE   7  (bases 1 to 8445)
  AUTHORS   Tan,J., D'Agostaro,A.F., Bendiak,B., Reck,F., Sarkar,M.,
            Squire,J.A., Leong,P. and Schachter,H.
  TITLE     The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2-
            N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic
            DNA, localization to chromosome 14q21, expression in insect cells
            and purification of the recombinant protein
  JOURNAL   Eur J Biochem 231 (2), 317-328 (1995)
   PUBMED   7635144
REFERENCE   8  (bases 1 to 8445)
  AUTHORS   Kumar,R., Yang,J., Eddy,R.L., Byers,M.G., Shows,T.B. and Stanley,P.
  TITLE     Cloning and expression of the murine gene and chromosomal location
            of the human gene encoding N-acetylglucosaminyltransferase I
  JOURNAL   Glycobiology 2 (4), 383-393 (1992)
   PUBMED   1421759
  REMARK    Erratum:[Glycobiology 1999 Aug;9(8):ix]
REFERENCE   9  (bases 1 to 8445)
  AUTHORS   Hull,E., Sarkar,M., Spruijt,M.P., Hoppener,J.W., Dunn,R. and
            Schachter,H.
  TITLE     Organization and localization to chromosome 5 of the human
            UDP-N-acetylglucosamine:alpha-3-D-mannoside
            beta-1,2-N-acetylglucosaminyltransferase I gene
  JOURNAL   Biochem Biophys Res Commun 176 (2), 608-615 (1991)
   PUBMED   1827260
REFERENCE   10 (bases 1 to 8445)
  AUTHORS   Kumar,R., Yang,J., Larsen,R.D. and Stanley,P.
  TITLE     Cloning and expression of N-acetylglucosaminyltransferase I, the
            medial Golgi transferase that initiates complex N-linked
            carbohydrate formation
  JOURNAL   Proc Natl Acad Sci U S A 87 (24), 9948-9952 (1990)
   PUBMED   1702225
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CN409895.1, M55621.1,
            CA426780.1 and AC022413.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_002406.3.
            
            Summary: There are believed to be over 100 different
            glycosyltransferases involved in the synthesis of protein-bound and
            lipid-bound oligosaccharides.
            UDP-N-acetylglucosamine:alpha-3-D-mannoside
            beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme
            essential for the synthesis of hybrid and complex N-glycans. The
            protein, encoded by a single exon, shows typical features of a type
            II transmembrane protein. The protein is believed to be essential
            for normal embryogenesis. Several variants encoding the same
            protein have been found for this gene. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 6. Variants 1-23 all encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and experimental data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.221990.1,
                                           SRR1803613.203737.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000307826.5/ ENSP00000311888.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-449               CN409895.1         40-488
            450-2682            M55621.1           358-2590
            2683-2684           CA426780.1         12-13               c
            2685-8445           AC022413.4         130078-135838
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q35.3"
     gene            1..8445
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
                     /note="alpha-1,3-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase"
                     /db_xref="GeneID:4245"
                     /db_xref="HGNC:HGNC:7044"
                     /db_xref="MIM:160995"
     exon            1..127
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
                     /inference="alignment:Splign:2.1.0"
     exon            128..8445
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             254..1591
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
                     /EC_number="2.4.1.101"
                     /note="glcNAc-T I; N-glycosyl-oligosaccharide-glycoprotein
                     N-acetylglucosaminyltransferase I; mannosyl
                     (alpha-1,3-)-glycoprotein
                     beta-1,2-N-acetylglucosaminyltransferase"
                     /codon_start=1
                     /product="alpha-1,3-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase"
                     /protein_id="NP_002397.2"
                     /db_xref="CCDS:CCDS4458.1"
                     /db_xref="GeneID:4245"
                     /db_xref="HGNC:HGNC:7044"
                     /db_xref="MIM:160995"
                     /translation="MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPAPGRPPSVSALD
                     GDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGRVPTAAPPAQPRVPVTPA
                     PAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQAIASYGSAVT
                     HIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPAAVVVEDDLEVAPDF
                     FEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELW
                     AELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKL
                     NQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRD
                     SFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPLTWEGYDPSWN"
     regulatory      2658..2663
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
                     /note="hexamer: AATAAA"
     polyA_site      2684
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
                     /note="major polyA site"
     regulatory      5698..5703
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
                     /note="hexamer: GATAAA"
     polyA_site      5719
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
     regulatory      8426..8431
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
                     /note="hexamer: AATAAA"
     polyA_site      8445
                     /gene="MGAT1"
                     /gene_synonym="GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I;
                     GnTI; MGAT"
ORIGIN      
        1 gcagaaaggg ctggcgagtc gaaaggcgag gcggccgcgg cagcgcttgg gacgcgcctg
       61 ggcaccgggc tcgctccctg cgccccggag caggccaagt tcggggccag gacgtcggga
      121 ggacctggtg catggctgcc tcctaatccc atagtccaga ggaggcatcc ctaggactgc
      181 gggcaaggga gccgggcaag cccagggcag ccttgaaccg tcccctggcc tgccctcccc
      241 ggtgggggcc aggatgctga agaagcagtc tgcagggctt gtgctgtggg gcgctatcct
      301 ctttgtggcc tggaatgccc tgctgctcct cttcttctgg acgcgcccag cacctggcag
      361 gccaccctca gtcagcgctc tcgatggcga ccccgccagc ctcacccggg aagtgattcg
      421 cctggcccaa gacgccgagg tggagctgga gcggcagcgt gggctgctgc agcagatcgg
      481 ggatgccctg tcgagccagc gggggagggt gcccaccgcg gcccctcccg cccagccgcg
      541 tgtgcctgtg acccccgcgc cggcggtgat tcccatcctg gtcatcgcct gtgaccgcag
      601 cactgttcgg cgctgcctgg acaagctgct gcattatcgg ccctcggctg agctcttccc
      661 catcatcgtt agccaggact gcgggcacga ggagacggcc caggccatcg cctcctacgg
      721 cagcgcggtc acgcacatcc ggcagcccga cctgagcagc attgcggtgc cgccggacca
      781 ccgcaagttc cagggctact acaagatcgc gcgccactac cgctgggcgc tgggccaggt
      841 cttccggcag tttcgcttcc ccgcggccgt ggtggtggag gatgacctgg aggtggcccc
      901 ggacttcttc gagtactttc gggccaccta tccgctgctg aaggccgacc cctccctgtg
      961 gtgcgtctcg gcctggaatg acaacggcaa ggagcagatg gtggacgcca gcaggcctga
     1021 gctgctctac cgcaccgact ttttccctgg cctgggctgg ctgctgttgg ccgagctctg
     1081 ggctgagctg gagcccaagt ggccaaaggc cttctgggac gactggatgc ggcggccgga
     1141 gcagcggcag gggcgggcct gcatacgccc tgagatctca agaacgatga cctttggccg
     1201 caagggtgtg agccacgggc agttctttga ccagcacctc aagtttatca agctgaacca
     1261 gcagtttgtg cacttcaccc agctggacct gtcttacctg cagcgggagg cctatgaccg
     1321 agatttcctc gcccgcgtct acggtgctcc ccagctgcag gtggagaaag tgaggaccaa
     1381 tgaccggaag gagctggggg aggtgcgggt gcagtatacg ggcagggaca gcttcaaggc
     1441 tttcgccaag gctctgggtg tcatggatga ccttaagtcg ggggttccga gagctggcta
     1501 ccggggtatt gtcaccttcc agttccgggg ccgccgtgtc cacctggcgc ccccactgac
     1561 gtgggagggc tatgatccta gctggaatta gcacctgcct gtccttcctg ggcccctcct
     1621 tgccacatca tgagctgagg tgggaccaca gtccccaggc tgcatcggcc tgcctgtgtt
     1681 tccctcttag gtgcatttat ctttttgatt tttccgagtg gcatttaagt gcacaaatga
     1741 taacaagagg attattctcc cgttctcaag ggagtcagat caggggaact attctagggt
     1801 atgttgcggg gtattaagca ggaaaccact gtgtggtggg gggcactggg cttgttgggg
     1861 ccagaaatgt ccacgtcctg agctttctcc tggagcatgt gcagagagtt tggcaacgtt
     1921 cgctctcttg accagacccc ttctccctga cctggctctt ccagccaggg cacgagccct
     1981 ccttctatac ctgctcccct tcccccagtg gggactgagt tatgggagaa ggggacatat
     2041 ttgtggccaa aatgatacta accaaagggg cttccttgtc agggcctggt ggagttggtg
     2101 ggtcatcggg gctcactgcc tcctgccctt ctctcctgtc tgacccccac ttagcccttc
     2161 tctccttgca gcctagcagt ttatagttct gagatggaaa gttgaagggg gcaagcaaga
     2221 cctctcctca gcccatgccc agctgtcagg agagaggtgc agggaggaag gccttgtgct
     2281 gggacaacct ctctcttgcc ttacctcaga gagggactat gccctgaccc ctcctttctg
     2341 aaaatcagtg ccctccctgt tgctctagga ggctcctgct ggcttggtag aagacagaat
     2401 tcgatctgcc tgtccctttt tcccctgggg tttgacacac aggctcctct cagcatgagg
     2461 tggagcagtg accaggtgga gcagtgacca ggacgcctct ggcccagtgc tgcccagcct
     2521 ccccgcccgc tcccaggcgc cccatgtcct cacaggccag gacgccatgg caggatggag
     2581 aggacttggt ggatttttgt ttcttgcctg acctcagttt catgaaagaa agtggaagct
     2641 acagaattat tttctaaaat aaaggctgaa ttgtctgaaa aatatttatg tgtgtgtgtc
     2701 ctggaaaagg aggtggcagg cagggaaaga aaggaaaagg gagaatgaag agttaaggag
     2761 agggctagac gggtgggagg aagcaaagtt gaggaaagaa agcagatgag tgggagggaa
     2821 gagccaggga cagcccaggg gatgggggcc aggtgggaaa ggaagtccag gccacaaggt
     2881 ggagaggagc cccggactgt gctcctgctg atcgtcctgt agagccagga ggccgcagca
     2941 ggaaagctgg tgagtgtctc ctgcttctgc ctcctgctgg ctgggcaggc ctggtctggg
     3001 gaagaaccag gtcctctgat cagggtggtc cctgagtggt gctttgtgcc cggtgccctc
     3061 tgctggccca ggcagtgctt attgagtgcc ctttatgacg tgccggggag cctcagggta
     3121 cacagctgat accagggttg gcctcacagt ggcgcttgga ctcccaaacg caaccccagt
     3181 gagagtgcct gttttgctaa tacccagaac acagacagac tgttacctct gcatatcacg
     3241 tgcaacattc tgagagtttc tgttctccac taattcattt aaaagaacaa aacgcggccg
     3301 ggcgcagtgg cttacacatg taatcccagc actttgggag gctgaggcgg acggatcacg
     3361 aagtcaggag ttcgggacca tcctggccaa catggtgaaa cctaaaaata ctctactaaa
     3421 aatacaaaaa cttgctgggc atggtggcac acacctgtaa tcccagctac tcaggaagct
     3481 gaggctggag aatcacttga acccgggagg cagaggttgc agtaatccaa gatcatacca
     3541 ctgcactcca gcctgggcga cagagcaaga ctctgtctcg gaaaaacaaa acgctttttg
     3601 tgactcacga acttgatttc atgagtgggt ttgaaaaccc tgggctagtc caggcgcggt
     3661 ggctcacgcc tgtaatccca gtactttggg aggctgaggc gggtggatca cttcaggagt
     3721 ttgagaccag cctggctaac atggtgaaac cccatctcta ctaaaaatac aaaaattagc
     3781 tgggtgtggt ggcacatgcc tgtaattcca gctacttggg aggctgaggc aggagaattg
     3841 cttgaacccg ggacggggtg gttgcagtga cctgagatcg caccactgca ctccagcctg
     3901 ggcggtaaga gcgaaactgc gtctcaaaac aaaacaaacc aaaaaccctg ggctaggcaa
     3961 ctttcttggc agaaactact ttggagctcg acaccagatg gtggcagagt tgtctgggga
     4021 gctgtgtcga gggccgtggc cttgggagca gctccaacct cccatctggg ctcagaaacc
     4081 agtgtcagac attggtttta ttttaacctt tgagatttgt gtggagcggg ttttgccttc
     4141 ccctgttgct tagatggatt atttagccat attttttgtt aaataagggg aggtggtcat
     4201 tatccgtgat ttcagacgtg gaatcggccc ggcgatagct gcattgtgcc ctttccggtg
     4261 gagccaggag gagtttggcc gccacgctcc atgcgcatag catgtcatgc agctagccaa
     4321 gtgcattgtt ctgagtgctc caggataagt accaacttac ttagtgctcc aggataagta
     4381 ccaacttagt gctccaggat aagtaccaac ttacttagtg ctccaggata agtaccaact
     4441 tacttagtgc tccaggataa gtaccaactt acttagtgct ccaggataag taccaactta
     4501 cttagtgctc caggataagt accaacttac ttagtgctcc aggataagta ccaacttact
     4561 tagtgctcca ggataagtac caacttactt gatcctcaga gcaagcctag gcggtgggta
     4621 ctgttttgct cctgttttac cagtgaggaa gcaggcacac agaggtgaag ttgcccaagg
     4681 tcacacagat ggttgaggga gagccggatt ggaatgaact ctgtgctgtt agccactgtg
     4741 tgaggctgga ctgttccatt ccagccttta cggcctcttc caggccattc cccagctgca
     4801 cttggattct caggccaggc accggcggtc tcctgtgagg gtggagagga agagggggct
     4861 gcgtggctcg gggaggtcca ggagagatgg cgggaggagt agagtttggg gcctgcggtg
     4921 ccagaggacc cccttacaag acgaaagact gggttctcag gctcctgaag caaggagcac
     4981 cttcaggggt ctcctggtat tcacccatca aggcttgctc aggtggcatg cgtgtgtgcg
     5041 tggcggctgc ccccgggccc ggaagcgcct gtggatacca aagcaagtgg aaatgccaga
     5101 tggaaaagcc ccgtgttagt caatgctgta gagttcagcc agcctcgagt ttgcaggttt
     5161 acagagctgc cttgcgcctg gctgctgctg ctgctgcatt cctgcttctg cctccagctg
     5221 tgaccacctc ctgccactcc tgctctcact gtgggttcct gctcactctg tctccaggac
     5281 agactgcctt ccgggcagcc ttccctcctc aagcctcttg gcctttgcaa tcctcttacc
     5341 tgggtcactg gtgggatctc atttgtggat caagtcgccc ccagtggaga caaatgtctg
     5401 atggagtctg gccaaggccc tcctgagcct tcactgggcc ctgcatgaga agcctgccaa
     5461 ggctgagggg ccagggaaac ggagacgaag ccctcagagc aggccaagag ggtgggaggt
     5521 ggggggcatt tagagggtta gatacagagc acccctttac aaataccagc tactggcttt
     5581 tcgtattaca tgttcatttt agatacaaca tactgcacac gctcccttaa cgtgtgttta
     5641 cgcgtgggtg gtgtatcctt ccagacgtct tcctcacatc tatacataat atgcatagat
     5701 aaaacattta ccaccaaaac ccatagtgtt atgtacatat ttgtctggct gtgtaacttt
     5761 aaataacata caaatgtata gaacagaagc ttgccctctt tctgaaatat ctaacgtggg
     5821 atttcaggct ttctgatgag acctctgtag aggtcagtgt tttgccacca gaggtccttc
     5881 agcctccatc tgttctccca cctacccaca ttctggcccc tggcaattca cagggagttt
     5941 aggaagtaga caccagggcc ctatttcctg gagaccagcc tggtggccga tgtcatagag
     6001 tgcggtgggc ctgggccggc cctgctctgg gcccgtgacc tgcgtgtctg actcgggtcc
     6061 agctgttctt cctgccagag cctcttcctg tgaggcgggg gtaacacaac cttctgtggt
     6121 ggctgtggga ttaatccgta tggctgcatt gtgagtaaag cctggtggaa atctctgtct
     6181 ccactgcttt ccttttcctg ctgttcacct tggctccctc gacacttaat ccccctccac
     6241 atgtgtctgg agcgggcaga gcgggggcag ttgatagaaa gggggccggg tagtgccaag
     6301 gctgttactt tcccccactg cctcccttgg ctaacttgaa gtctgaccct ttataccaga
     6361 actgtaagac agtgtgtcag aatggattca gttgcagagc aggaagcctg gatcccagcg
     6421 ccggacggaa atgagaggtc gatggtgcag gctggtgtgg gggttcctcc accgtcaggg
     6481 cccaggctcc ttctcaggtc tcagtctcac tcttcttagc acgaggtcag gtctcattcc
     6541 ttggctgcgg gagctccagc cagcacacgt tttaggaaga aggaggaagg aagaaggtaa
     6601 agggtggatg gcagctgcct cctcctctct tggaagctca cagagcgctg ggtcacaatt
     6661 ccctgggtag aactggatca cacaggcacc cctgtcagca aaagaggctg gaaaaagtat
     6721 ttctgaagct gaatcaaatt aagattcctg ttactaagaa gggagtgatt gtatgggcga
     6781 ctacagggac cacggccata cacggtgcct ggaagaccaa gcttgtgaca gtgacgagga
     6841 tttggagagg gcagggtgat ggggagcttt ggggggagtg cattaggaac cctgacctgg
     6901 agaggggcag aatgaaacct gcgggcccct aaccctagtg ggttgtttga gaaacctgtc
     6961 ctactgtggc agggggctga gtcttctcag ggtcatgcat gaaaactgaa attcctgaag
     7021 gccacacaca gccccggaag tgctgactga caagggcaca tgccagtgat gcagttttct
     7081 tttttgtctt cacttttgtt gttccgccgt cttcaacaca tggctcccag ctcacagcaa
     7141 atgctgccct ggttcagcta ccaggaagca ggagagggga agggagggca agcctcattc
     7201 tttatggaac aaaagcagtg ggcagacagc cttccctcac gttccactca ctggaatgtg
     7261 gtctggctgc cttccctgca ggaggtggga tgcaccctca gtgcagcagc cctgccccag
     7321 ccaaaaacgt ctcagcacag ctgtaagaag aggaagatgg ctagagacag gcagccttcc
     7381 ctgcaagggc aatgaggcaa gcctgccaaa agcctccttt ggaaatactg tttgattact
     7441 tttcagagtt tctatttagg aaaagggcaa aacctttcag gatcaggagt aggtgagcgg
     7501 cgatcaggcc tcactcttca ccgagggcct tccctgagga ggggcctgca ggacgctggc
     7561 agcggaatgg ggctgtgtgg ctgctagatt aggtcatggc agggagacag cctcttcctg
     7621 gttcttgcta gaacaggcac acttggggcc ttgagctcca gggagttcat ggaagacatc
     7681 tgactaccct gagccccttg ctggagggtt ctggggagtg gcccacggcc accgtcaccc
     7741 atggccaccg tcacccacag gggccgtgag gacatccctg gccagcatct aacacaacca
     7801 catgggatgc cctaagcaac tactgcccag ccgagggctg cctgaatttc tgactcacaa
     7861 atggtgggca atgtgaaacg ggtattttaa gctgctgagt tctgcgggag tttgctgagc
     7921 aacagtagaa accagaactc tcggatactg tgttctgtcc ttgaaacggt ggaagatttt
     7981 atgtgaccag aatggaatgt ttgaacaaat attttgagaa tagaatttta aaacatttac
     8041 tcatcagcat ccaagggtgg ggagcagtgt catctaggag actggttcta ttatttacgt
     8101 agatgttctt gaattaaagg atgggaatat tttttccttc caaaactttt tttcaatttt
     8161 atattctgtt tttcaagttt ttattatgaa aatattcaaa ctcacaggga agttgaaaga
     8221 ctaatacaaa gaagaaccat actctttcca ctgacatgat ccaacgagga atattttgcc
     8281 acacggcaaa tatataccgt ataattttct gtaccacttg atagaaaggt aaagaatgaa
     8341 tattgttttt taaataactt ttttcgtata taagtaatat gtgcttatcg tgtaactacc
     8401 taaaaacatt gaaaaataaa aggaaaataa aattcataca taacc
//
