LOCUS       NM_032889               1763 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens major facilitator superfamily domain containing 5
            (MFSD5), transcript variant 2, mRNA.
ACCESSION   NM_032889
VERSION     NM_032889.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Rogers,M.A., Bartoli-Leonard,F., Zheng,K.H., Small,A.M., Chen,H.Y.,
            Clift,C.L., Asano,T., Kuraoka,S., Blaser,M.C., Perez,K.A.,
            Natarajan,P., Yeang,C., Stroes,E.S.G., Tsimikas,S., Engert,J.C.,
            Thanassoulis,G., O'Donnell,C.J., Aikawa,M., Singh,S.A. and
            Aikawa,E.
  TITLE     Major Facilitator Superfamily Domain Containing 5 Inhibition
            Reduces Lipoprotein(a) Uptake and Calcification in Valvular Heart
            Disease
  JOURNAL   Circulation 149 (5), 391-401 (2024)
   PUBMED   37937463
  REMARK    GeneRIF: Major Facilitator Superfamily Domain Containing 5
            Inhibition Reduces Lipoprotein(a) Uptake and Calcification in
            Valvular Heart Disease.
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1763)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1763)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 1763)
  AUTHORS   Tejada-Jimenez,M., Galvan,A. and Fernandez,E.
  TITLE     Algae and humans share a molybdate transporter
  JOURNAL   Proc Natl Acad Sci U S A 108 (16), 6420-6425 (2011)
   PUBMED   21464289
REFERENCE   6  (bases 1 to 1763)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 1763)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA428788.1, DA695979.1, AK074684.1 and BC067795.1.
            
            On Jun 2, 2019 this sequence version replaced NM_032889.4.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1. The encoded isoform
            (2) is shorter at the N-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY358604.1, BC067795.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000329548.5/ ENSP00000332624.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 DA428788.1         4-9
            7-125               DA695979.1         7-125
            126-661             AK074684.1         126-661
            662-1763            BC067795.1         593-1694
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.13"
     gene            1..1763
                     /gene="MFSD5"
                     /gene_synonym="hsMOT2; SLC61A1"
                     /note="major facilitator superfamily domain containing 5"
                     /db_xref="GeneID:84975"
                     /db_xref="HGNC:HGNC:28156"
                     /db_xref="MIM:620345"
     exon            1..174
                     /gene="MFSD5"
                     /gene_synonym="hsMOT2; SLC61A1"
                     /inference="alignment:Splign:2.1.0"
     exon            175..1763
                     /gene="MFSD5"
                     /gene_synonym="hsMOT2; SLC61A1"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..1545
                     /gene="MFSD5"
                     /gene_synonym="hsMOT2; SLC61A1"
                     /note="isoform 2 is encoded by transcript variant 2; major
                     facilitator superfamily domain-containing protein 5;
                     molybdate transporter 2 homolog; molybdate-anion
                     transporter"
                     /codon_start=1
                     /product="molybdate-anion transporter isoform 2"
                     /protein_id="NP_116278.3"
                     /db_xref="CCDS:CCDS8851.1"
                     /db_xref="GeneID:84975"
                     /db_xref="HGNC:HGNC:28156"
                     /db_xref="MIM:620345"
                     /translation="MLVTAYLAFVGLLASCLGLELSRCRAKPPGRACSNPSFLRFQLD
                     FYQVYFLALAADWLQAPYLYKLYQHYYFLEGQIAILYVCGLASTVLFGLVASSLVDWL
                     GRKNSCVLFSLTYSLCCLTKLSQDYFVLLVGRALGGLSTALLFSAFEAWYIHEHVERH
                     DFPAEWIPATFARAAFWNHVLAVVAGVAAEAVASWIGLGPVAPFVAAIPLLALAGALA
                     LRNWGENYDRQRAFSRTCAGGLRCLLSDRRVLLLGTIQALFESVIFIFVFLWTPVLDP
                     HGAPLGIIFSSFMAASLLGSSLYRIATSKRYHLQPMHLLSLAVLIVVFSLFMLTFSTS
                     PGQESPVESFIAFLLIELACGLYFPSMSFLRRKVIPETEQAGVLNWFRVPLHSLACLG
                     LLVLHDSDRKTGTRNMFSICSAVMVMALLAVVGLFTVVRHDAELRVPSPTEEPYAPEL
                     "
     regulatory      1742..1747
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MFSD5"
                     /gene_synonym="hsMOT2; SLC61A1"
                     /note="hexamer: AATAAA"
     polyA_site      1763
                     /gene="MFSD5"
                     /gene_synonym="hsMOT2; SLC61A1"
                     /note="major polyA site"
ORIGIN      
        1 acgtgatgaa gccatcatgg cggcggccag aggcctgccc ggctcccgga agcaggctgt
       61 gaggggcggg agcgctgctg gaacccgagc cggagccgga gccacagcgg ggagggtggc
      121 ctggcggcct ggagccggac gtgtccgggg cgtccccgca gaccggggca gcaggtcgtc
      181 cgggggccca ccatgctggt gactgcctac cttgcttttg taggcctcct ggcctcctgc
      241 ctggggctgg aactgtcaag atgccgggct aaaccccctg gaagggcctg cagcaatccc
      301 tccttccttc ggtttcaact ggacttctat caggtctact tcctggccct ggcagctgat
      361 tggcttcagg ccccctacct ctataaactc taccagcatt actacttcct ggaaggtcaa
      421 attgccatcc tctatgtctg tggccttgcc tctacagtcc tctttggcct agtggcctcc
      481 tcccttgtgg attggctggg tcgcaagaat tcttgtgtcc tcttctccct gacttactca
      541 ctatgctgct taaccaaact ctctcaagac tactttgtgc tgctagtggg gcgagcactt
      601 ggtgggctgt ccacagccct gctcttctca gccttcgagg cctggtatat ccatgagcac
      661 gtggaacggc atgacttccc tgctgagtgg atcccagcta cctttgctcg agctgccttc
      721 tggaaccatg tgctggctgt agtggcaggt gtggcagctg aggctgtagc cagctggata
      781 gggctggggc ctgtagcgcc ctttgtggct gccatccctc tcctggctct ggcaggggcc
      841 ttggcccttc gaaactgggg ggagaactat gaccggcagc gtgccttctc aaggacctgt
      901 gctggaggcc tgcgctgcct cctgtcggac cgccgcgtgc tgctgttggg caccatacaa
      961 gctctatttg agagtgtcat cttcatcttt gtcttcctct ggacacctgt gctggaccca
     1021 cacggggccc ctctgggcat tatcttctcc agcttcatgg cagccagcct gcttggctct
     1081 tccctgtacc gtatcgccac ctccaagagg taccaccttc agcccatgca cctgctgtcc
     1141 cttgctgtgc tcatcgtcgt cttctctctc ttcatgttga ctttctctac cagcccaggc
     1201 caggagagtc cggtggagtc cttcatagcc tttctactta ttgagttggc ttgtggatta
     1261 tactttccca gcatgagctt cctacggaga aaggtgatcc ctgagacaga gcaggctggt
     1321 gtactcaact ggttccgggt acctctgcac tcactggctt gcctagggct ccttgtcctc
     1381 catgacagtg atcgaaaaac aggcactcgg aatatgttca gcatttgctc tgctgtcatg
     1441 gtgatggctc tgctggcagt ggtgggactc ttcaccgtgg taaggcatga tgctgagctg
     1501 cgggtacctt cacctactga ggagccctat gcccctgagc tgtaacccca ctccaggaca
     1561 agatagctgg gacagactct tgaattccag ctatccggga ttgtacagat ctctctgtga
     1621 ctgactttgt gactgtcctg tggtttctcc tgccattgct ttgtgtttgg gaggacatga
     1681 tgggggtgat ggactggaaa gaaggtgcca aaagttccct ctgtgttact cccatttaga
     1741 aaataaacac ttttaaatga tca
//
