LOCUS       NM_181644               4058 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens major facilitator superfamily domain containing 4A
            (MFSD4A), mRNA.
ACCESSION   NM_181644
VERSION     NM_181644.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Perland,E., Hellsten,S.V., Schweizer,N., Arapi,V., Rezayee,F.,
            Bushra,M. and Fredriksson,R.
  TITLE     Structural prediction of two novel human atypical SLC transporters,
            MFSD4A and MFSD9, and their neuroanatomical distribution in mice
  JOURNAL   PLoS One 12 (10), e0186325 (2017)
   PUBMED   29049335
  REMARK    GeneRIF: propose MFSD4A and MFSD9 to be novel transporters,
            belonging to disparate SLC families. Both proteins were located to
            neurons in mouse brain, and their mRNA expression levels were
            affected by the diet
            Erratum:[PLoS One. 2018 May 10;13(5):e0197417. doi:
            10.1371/journal.pone.0197417.. PMID: 29746586]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Kanda,M., Shimizu,D., Tanaka,H., Shibata,M., Iwata,N., Hayashi,M.,
            Kobayashi,D., Tanaka,C., Yamada,S., Fujii,T., Nakayama,G.,
            Sugimoto,H., Koike,M., Fujiwara,M. and Kodera,Y.
  TITLE     Metastatic pathway-specific transcriptome analysis identifies MFSD4
            as a putative tumor suppressor and biomarker for hepatic metastasis
            in patients with gastric cancer
  JOURNAL   Oncotarget 7 (12), 13667-13679 (2016)
   PUBMED   26872374
  REMARK    GeneRIF: Low MFSD4 expression is associated with hepatic metastasis
            in patients gastric cancer.
REFERENCE   3  (bases 1 to 4058)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC317463.1, AK091896.1, BC036549.2, AK090831.1 and BC016377.1.
            
            On Nov 23, 2018 this sequence version replaced NM_181644.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.235333.1,
                                           SRR1660803.81451.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367147.9/ ENSP00000356115.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                DC317463.1         31-59
            30-743              AK091896.1         1-714
            744-996             BC036549.2         724-976
            997-1984            AK091896.1         968-1955
            1985-1991           BC036549.2         1965-1971
            1992-2106           AK091896.1         1963-2077
            2107-3054           BC036549.2         2085-3032
            3055-4057           AK090831.1         1812-2814
            4058-4058           BC016377.1         937-937
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..4058
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /note="major facilitator superfamily domain containing 4A"
                     /db_xref="GeneID:148808"
                     /db_xref="HGNC:HGNC:25433"
                     /db_xref="MIM:620299"
     exon            1..286
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..1601
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /note="major facilitator superfamily domain-containing
                     protein 4; major facilitator superfamily domain containing
                     4"
                     /codon_start=1
                     /product="major facilitator superfamily domain-containing
                     protein 4A"
                     /protein_id="NP_857595.3"
                     /db_xref="CCDS:CCDS1455.1"
                     /db_xref="GeneID:148808"
                     /db_xref="HGNC:HGNC:25433"
                     /db_xref="MIM:620299"
                     /translation="MGCDGRVSGLLRRNLQPTLTYWSVFFSFGLCIAFLGPTLLDLRC
                     QTHSSLPQISWVFFSQQLCLLLGSALGGVFKRTLAQSLWALFTSSLAISLVFAVIPFC
                     RDVKVLASVMALAGLAMGCIDTVANMQLVRMYQKDSAVFLQVLHFFVGFGALLSPLIA
                     DPFLSEANCLPANSTANTTSRGHLFHVSRVLGQHHVDAKPWSNQTFPGLTPKDGAGTR
                     VSYAFWIMALINLPVPMAVLMLLSKERLLTCCPQRRPLLLSADELALETQPPEKEDAS
                     SLPPKFQSHLGHEDLFSCCQRKNLRGAPYSFFAIHITGALVLFMTDGLTGAYSAFVYS
                     YAVEKPLSVGHKVAGYLPSLFWGFITLGRLLSIPISSRMKPATMVFINVVGVVVTFLV
                     LLIFSYNVVFLFVGTASLGLFLSSTFPSMLAYTEDSLQYKGCATTVLVTGAGVGEMVL
                     QMLVGSIFQAQGSYSFLVCGVIFGCLAFTFYILLLFFHRMHPGLPSVPTQDRSIGMEN
                     SECYQR"
     exon            287..485
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     exon            486..746
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     exon            747..915
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1031
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1205
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1353
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1424
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1545
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..4058
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4040..4045
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /note="hexamer: AATAAA"
     polyA_site      4058
                     /gene="MFSD4A"
                     /gene_synonym="MFSD4; SLC60A1; UNQ3064"
                     /note="major polyA site"
ORIGIN      
        1 ggcactcggg caccgggctc gccgggacca gatccgcgag cccgtcagcc tgcgccatgg
       61 gctgcgacgg ccgcgtgtcg gggctgctcc gccgcaacct gcagcccacg ctcacctact
      121 ggagcgtctt cttcagcttc ggcctgtgca tcgccttcct ggggcccacg ctgctggacc
      181 tgcgctgtca gacgcacagc tcgctgcccc agatctcctg ggtcttcttc tcgcagcagc
      241 tctgcctcct gctgggcagc gccctcgggg gcgtcttcaa aaggaccctg gcccagtcac
      301 tatgggccct gttcacctcc tctctggcca tctccctggt gtttgccgtc atccccttct
      361 gccgcgacgt gaaggtgctg gcctcagtca tggcgctggc gggcttggcc atgggctgca
      421 tcgacaccgt ggccaacatg cagctggtaa ggatgtacca gaaggactcg gccgtcttcc
      481 tccaggtgct ccatttcttc gtgggctttg gtgctctgct gagccccctt attgctgacc
      541 ctttcctgtc tgaggccaac tgcttgcctg ccaatagcac ggccaacacc acctcccgag
      601 gccacctgtt ccatgtctcc agggtgctgg gccagcacca cgtagatgcc aagccttggt
      661 ccaaccagac gttcccaggg ctgactccaa aggacggggc agggacccga gtgtcctatg
      721 ccttctggat catggccctc atcaatcttc cagtgcccat ggctgtgctg atgctgctgt
      781 ccaaggagcg gctgctgacc tgctgtcccc agaggaggcc cctgcttctg tctgctgatg
      841 agcttgcctt ggagacacag cctcctgaga aggaagatgc ctcctcactg cccccaaagt
      901 ttcagtcaca cctagggcat gaggacctgt tcagctgctg ccaaaggaag aacctcagag
      961 gagcccctta ttccttcttt gccatccaca tcacgggcgc cctggtactg ttcatgacgg
     1021 atgggttgac gggtgcctat tccgccttcg tgtacagcta tgctgtggag aagcccctgt
     1081 ctgtgggaca caaggtggct ggctacctcc ccagcctctt ctggggcttc atcacactgg
     1141 gccggctcct ctccattccc atatcctcaa gaatgaagcc ggccaccatg gttttcatca
     1201 acgtggttgg cgtggtggtg acgttcctgg tgctgcttat tttctcctac aacgtcgtct
     1261 tcctgttcgt ggggacggca agcctgggcc tgtttctcag cagcaccttc cccagcatgc
     1321 tggcctacac ggaggactcg ctgcagtaca aaggctgtgc aaccacagtg ctggtgacag
     1381 gggcaggagt tggcgagatg gtgctgcaga tgctggttgg ttcgatattc caggctcagg
     1441 gcagctatag tttcctggtc tgtggcgtga tctttggttg tctggctttt accttctata
     1501 tcttgctcct gtttttccac aggatgcacc ctggactccc atcagttcct acccaagaca
     1561 gatcaattgg aatggaaaac tctgagtgct accagaggta aaactgggtg aagaaggcaa
     1621 gagaagactt tcagcctctt gatcaccagc acgaccatac tgtttcagaa agctgggtgg
     1681 tggtggaggc gctctctcaa tggctattca agtcttctcc actaaaactt ggttgggtag
     1741 aggaaattaa attgagtcct ggtacctggt caaaatcatt agaagtttac ctggcttctc
     1801 aagttatctt cttccctggt tcagactgtt ggtaagagct gtccagatac ccagatggga
     1861 aggaaggaga cagccgcgcg cttcactcca tttgtcacct catgcatgga ccatactctg
     1921 ggtttgagat cattcttcat tgaagtttgt aaaaataggt tgaaattgta aagctccatg
     1981 atcattgcta tatgtagata tatttcaatt taagcaaaac aagctgcaag ttattccctg
     2041 gcatgctcaa aggattttcg tgcttttcac ttaatagtcc aaagtctctt aaattcctgc
     2101 tgcagacatc aatagcttat ctatattctc aaacaccaaa aggaaaagtt gaatcttgct
     2161 ctctttggta tactaatgta gtggtatgct aagctggctc ataccaactt agaaaagctg
     2221 attgtaaaat tttcattttg acagctggtt attaaatgca gccattatta aaaatcaaat
     2281 catacaaact tataattaaa tcaattacat ttaaaacaaa ggtaataaat attcaaagca
     2341 tatcacttcc taatttgatc ttgatgctct tgaggtaatt tacgtccatg gtacctgtgt
     2401 ggtggaatta ctatatatga tggtgtgcta ctgtgcacct tgtctcaact ccactcttcg
     2461 tgatagcatg ttggtagctt gaaatcagcc tggtgggagt attaccatgg acactggcaa
     2521 aagctacaga tcccggagag ccagtggtta aacatttacc agcataccac tgctagtaat
     2581 caaggctaac tggtccagaa atcgcccagg agaatgaaat ggatgttcca tttttttcta
     2641 ctgacattga ctagcatata aaaggtatag aaacagcact aagactttct gaaaatacct
     2701 aatgaaaatt ttacatcttt ttttgttttt gtttttgttt ttgagacaga gtctcactct
     2761 gttgccaggc tggaatgcag tggcgtcatc tcggctcact gcaacctccg cctcccagtt
     2821 tcaagcgatt ctcctgcctc agcctcccaa gtagctgtga ctacaggcgc atgccatcac
     2881 gcacagctaa ttttttgtat ttttagtaga gacgggattt caccgtgtta gccaggatgg
     2941 tcttgatctc ctgacctcat gatctgcccg tctcggcctc ccaaagtgct gggattacag
     3001 gcgtgagcca ctgcacccag ccaaaaattt tacatctttt atagagggaa aaaaactctt
     3061 tataccatgg caaggccttt tctttcacaa aaagctgggc ctactgaaca attcaagctg
     3121 tgcagtagta gactgaaagc aggatttgtt gaggagttac agctcctgtc cagagcaaat
     3181 cctgtagtga tacaaggaga atgtaaactt gccagcttag acagggatca gtcctgagac
     3241 tgctggcagt agcaaatggc tattagagta actgtataat ggttttgcct gcactttctc
     3301 tatgtatata caaatgtaca tgtataaata taaaaattaa gtgatcatgg ttcttggtaa
     3361 cctgtcccaa gtgctgtgat tcacacgcct gacactaaaa ggttcttcct ggtccagtca
     3421 gccagctgtg accaccagca gcacagctga gtgctgagaa tctggctgga aagagaaacg
     3481 tggctcaagt gctggctcac cttctagctg tgtggccctg ggcaggttac tgaggctctt
     3541 ccaacctcac ttttcacatg taaaatggca tttctaaaag tacctaccct acctcaaagg
     3601 aattctgaaa aaagtaagtg aatttgtata aagcaaagca ctcacaatat gcctgacaca
     3661 caaactgctt aataaatgtt agtaattttt aaaacctcct cataagcggc ttagaaatta
     3721 ttcatgtttg gtacatattt attgcaagtc aagcagatcc aacttttttg aaagctggtg
     3781 gtctaggagt atagttttaa ttatatttgt aaatactcaa aagtattaga tgccagtcta
     3841 atttaaatgc agtctgactg tagcgagtaa ctgacctggg caatgaaaaa gcagttgtat
     3901 ttttcttgtg gtttttcaag aaaatatcct ttttatacag atttagaaag aagaatcaat
     3961 ggctatatcg aagttgattg tgactaattt gttaatacag aaaacaacat taacacaaat
     4021 ctaagagtac ctttcctaaa ataaaatatt taaactta
//
