LOCUS       NM_002405               2073 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens MFNG O-fucosylpeptide
            3-beta-N-acetylglucosaminyltransferase (MFNG), transcript variant
            1, mRNA.
ACCESSION   NM_002405
VERSION     NM_002405.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Gao,Y., Luo,L., Qu,Y. and Zhou,Q.
  TITLE     MFNG is an independent prognostic marker for osteosarcoma
  JOURNAL   Eur J Med Res 28 (1), 256 (2023)
   PUBMED   37496053
  REMARK    GeneRIF: MFNG is an independent prognostic marker for osteosarcoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Cheng,W.K., Kaur,G., Sjoberg,E., Frodin,M., Egevad,L.,
            Harmenberg,U., Li,J.L. and Oon,C.E.
  TITLE     Nuclear and stromal expression of Manic fringe in renal cell
            carcinoma
  JOURNAL   Exp Mol Pathol 122, 104667 (2021)
   PUBMED   34371013
  REMARK    GeneRIF: Nuclear and stromal expression of Manic fringe in renal
            cell carcinoma.
REFERENCE   3  (bases 1 to 2073)
  AUTHORS   Lopez-Arribillaga,E., Rodilla,V., Colomer,C., Vert,A., Shelton,A.,
            Cheng,J.H., Yan,B., Gonzalez-Perez,A., Junttila,M.R., Iglesias,M.,
            Torres,F., Albanell,J., Villanueva,A., Bigas,A., Siebel,C.W. and
            Espinosa,L.
  TITLE     Manic Fringe deficiency imposes Jagged1 addiction to intestinal
            tumor cells
  JOURNAL   Nat Commun 9 (1), 2992 (2018)
   PUBMED   30065304
  REMARK    GeneRIF: MFNG imposes a negative correlation between Jag1 and
            Notch, being high Jag1 in the absence of MFNG predictive of poor
            prognosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2073)
  AUTHORS   Zhang,S., Chung,W.C., Wu,G., Egan,S.E., Miele,L. and Xu,K.
  TITLE     Manic fringe promotes a claudin-low breast cancer phenotype through
            notch-mediated PIK3CG induction
  JOURNAL   Cancer Res 75 (10), 1936-1943 (2015)
   PUBMED   25808869
  REMARK    GeneRIF: Mfng is an oncogene acting through Notch-mediated
            induction of Pik3cg.
REFERENCE   5  (bases 1 to 2073)
  AUTHORS   Davila,S., Froeling,F.E., Tan,A., Bonnard,C., Boland,G.J.,
            Snippe,H., Hibberd,M.L. and Seielstad,M.
  TITLE     New genetic associations detected in a host response study to
            hepatitis B vaccine
  JOURNAL   Genes Immun 11 (3), 232-238 (2010)
   PUBMED   20237496
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 2073)
  AUTHORS   Moran,J.L., Johnston,S.H., Rauskolb,C., Bhalerao,J., Bowcock,A.M.
            and Vogt,T.F.
  TITLE     Genomic structure, mapping, and expression analysis of the
            mammalian Lunatic, Manic, and Radical fringe genes
  JOURNAL   Mamm Genome 10 (6), 535-541 (1999)
   PUBMED   10341080
REFERENCE   7  (bases 1 to 2073)
  AUTHORS   Van Tine,B.A., Knops,J., Shaw,G.M. and May,W.A.
  TITLE     Assignment of human MFNG, manic fringe Drosophila homolog, to
            22q13.1 using tyramide fluorescence in situ hybridization (T-FISH)
  JOURNAL   Cytogenet Cell Genet 87 (1-2), 132-133 (1999)
   PUBMED   10640833
REFERENCE   8  (bases 1 to 2073)
  AUTHORS   Egan,S., Herbrick,J.A., Tsui,L.C., Cohen,B., Flock,G., Beatty,B.
            and Scherer,S.W.
  TITLE     Mapping of the human Lunatic Fringe (LFNG) gene to 7p22 and Manic
            Fringe (MFNG) to 22q12
  JOURNAL   Genomics 54 (3), 576-577 (1998)
   PUBMED   9878264
REFERENCE   9  (bases 1 to 2073)
  AUTHORS   Cohen,B., Bashirullah,A., Dagnino,L., Campbell,C., Fisher,W.W.,
            Leow,C.C., Whiting,E., Ryan,D., Zinyk,D., Boulianne,G., Hui,C.C.,
            Gallie,B., Phillips,R.A., Lipshitz,H.D. and Egan,S.E.
  TITLE     Fringe boundaries coincide with Notch-dependent patterning centres
            in mammals and alter Notch-dependent development in Drosophila
  JOURNAL   Nat Genet 16 (3), 283-288 (1997)
   PUBMED   9207795
REFERENCE   10 (bases 1 to 2073)
  AUTHORS   Johnston,S.H., Rauskolb,C., Wilson,R., Prabhakaran,B., Irvine,K.D.
            and Vogt,T.F.
  TITLE     A family of mammalian Fringe genes implicated in boundary
            determination and the Notch pathway
  JOURNAL   Development 124 (11), 2245-2254 (1997)
   PUBMED   9187150
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC094814.1 and AI363008.1.
            
            On May 17, 2019 this sequence version replaced NM_002405.3.
            
            Summary: This gene is a member of the glycosyltransferase 31 gene
            family. Members of this gene family, which also includes the LFNG
            (GeneID: 3955) and RFNG (GeneID: 5986) genes, encode evolutionarily
            conserved glycosyltransferases that act in the Notch signaling
            pathway to define boundaries during embryonic development. While
            their genomic structure is distinct from other
            glycosyltransferases, these proteins have a fucose-specific
            beta-1,3-N-acetylglucosaminyltransferase activity that leads to
            elongation of O-linked fucose residues on Notch, which alters Notch
            signaling. The protein encoded by this gene may control Notch
            signaling in claudin-low breast cancer. [provided by RefSeq, May
            2018].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.59001.1,
                                           SRR1163655.244209.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356998.8/ ENSP00000349490.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2068              BC094814.1         18-2085
            2069-2073           AI363008.1         1-5                 c
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.1"
     gene            1..2073
                     /gene="MFNG"
                     /note="MFNG O-fucosylpeptide
                     3-beta-N-acetylglucosaminyltransferase"
                     /db_xref="GeneID:4242"
                     /db_xref="HGNC:HGNC:7038"
                     /db_xref="MIM:602577"
     exon            1..462
                     /gene="MFNG"
                     /inference="alignment:Splign:2.1.0"
     CDS             208..1173
                     /gene="MFNG"
                     /EC_number="2.4.1.222"
                     /note="isoform 1 is encoded by transcript variant 1;
                     beta-1,3-N-acetylglucosaminyltransferase manic fringe;
                     O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase"
                     /codon_start=1
                     /product="beta-1,3-N-acetylglucosaminyltransferase manic
                     fringe isoform 1"
                     /protein_id="NP_002396.2"
                     /db_xref="CCDS:CCDS13947.1"
                     /db_xref="GeneID:4242"
                     /db_xref="HGNC:HGNC:7038"
                     /db_xref="MIM:602577"
                     /translation="MQCRLPRGLAGALLTLLCMGLLCLRYHLNLSPQRVQGTPELSQP
                     NPGPPKLQLHDVFIAVKTTRAFHRLRLELLLDTWVSRTREQTFVFTDSPDKGLQERLG
                     SHLVVTNCSAEHSHPALSCKMAAEFDTFLASGLRWFCHVDDDNYVNPRALLQLLRAFP
                     LARDVYVGRPSLNRPIHASEPQPHNRTRLVQFWFATGGAGFCINRKLALKMAPWASGS
                     RFMDTSALIRLPDDCTMGYIIECKLGGRLQPSPLFHSHLETLQLLRTAQLPEQVTLSY
                     GVFEGKLNVIKLQGPFSPEEDPSRFRSLHCLLYPDTPWCPQLGAR"
     exon            463..511
                     /gene="MFNG"
                     /inference="alignment:Splign:2.1.0"
     exon            512..614
                     /gene="MFNG"
                     /inference="alignment:Splign:2.1.0"
     exon            615..768
                     /gene="MFNG"
                     /inference="alignment:Splign:2.1.0"
     exon            769..854
                     /gene="MFNG"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1020
                     /gene="MFNG"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1106
                     /gene="MFNG"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..2073
                     /gene="MFNG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2048..2053
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MFNG"
                     /note="hexamer: ATTAAA"
     polyA_site      2073
                     /gene="MFNG"
                     /note="major polyA site"
ORIGIN      
        1 agcaggtgga ggactggcgg cggtcgagat cgtgctgcca tggctcagcc tctgggtcca
       61 gagcctcagc tcctacctct tccctccttg ccagcccctg atgcctgcca gacttttgcc
      121 tctgctggag cccctgcctg accagcttcc cctccctgtc tggttgggat ttgggggctg
      181 agctgtctgg ggtcccaggg ccaaccaatg cagtgccggc tcccgcgggg cctggctgga
      241 gccctcctca ccctcctgtg catggggctc ctgtgtctgc ggtaccactt gaacctgtcc
      301 ccgcagcggg tacaagggac ccccgagctg agccagccga acccggggcc ccctaagcta
      361 cagctacacg atgtcttcat tgcagtgaag acgacccggg ctttccaccg cttgcgcctg
      421 gagctgctgc ttgacacgtg ggtttccagg accagggaac agacatttgt cttcaccgac
      481 agcccagaca aaggcctcca ggagagactg gggtcccacc ttgtggtcac caactgctcc
      541 gcggaacaca gccacccagc tctgtcctgc aagatggctg ctgagttcga caccttcttg
      601 gccagtgggc ttaggtggtt ctgccatgtg gacgatgaca actatgtgaa cccaagggcg
      661 ctgctgcagc ttctgagagc cttcccgctg gcccgcgacg tctatgtggg aaggcccagc
      721 ctgaaccggc ccatccatgc ctcagagcca cagccccaca accgcacgag gctggtacag
      781 ttctggtttg ccactggggg tgctggcttc tgcatcaatc gcaaactggc tttgaagatg
      841 gctccgtggg ccagtggctc ccgtttcatg gacacatctg ctctcatccg gctgcctgat
      901 gactgcacca tgggctatat cattgagtgc aagctgggcg gccgcctgca gcccagcccc
      961 ctctttcact cccacctgga gaccctgcag ctgctgagga ctgcacagct cccagaacag
     1021 gtcaccctca gctacggtgt ctttgagggg aaactcaacg tcattaagct acagggcccc
     1081 ttctccccgg aggaggaccc ctccagattt cgctccctcc attgtctgct ctatccagat
     1141 acaccctggt gtccccagct gggtgcccga tgaatcctga actgctgggc aaaggttggg
     1201 cagagacttc tgggtgtgcc ttggctccca aggtggcact gtgggtccct ggcaagtgtc
     1261 ttgtgatagg cagtccctgg cagggccttc gggtggttgg caagcccagg atctgagtgg
     1321 caattggcac tgaaggcacc ccaggcccct gggaggtgag ttagacagcc caggggacca
     1381 ggtggaccag gtggtggcca gagaggctcc aggggctaga ctccctcagg aggctgaatt
     1441 gaaaaagggc agggggcact tgagctgggc tggggctcag gggtcctaac cctttaggca
     1501 gtgacatggc ctctgggtgg ggtctggccg ttggccctgg ctaatgtctc tcagtcattc
     1561 ccctggggct caagcgctgg gccgcccact cctgcctccc tcatctgtgt cccgagttcc
     1621 tgaagggaca tgggtggaat gatggcagaa tccagggtct gcagcacctg ctgttgttgc
     1681 caaccagtct cccaaagctc cttgctcccc accccttgcg aacaggacca gattttgttt
     1741 ggagcctcag catgccgggg cccagatgat ggagcataac gggtcccagc caattgtgat
     1801 gatccttttt gctcatttcc cagcctttct tgctgttagg ggctaccatg ggaccagctc
     1861 tggccagagg gaactaagca aatccaatag agatgtttct ggggaaggtt ttgcagccca
     1921 ctccccatct tcctgctata aatgtgggtg tgatggctgg atctggggca gccaccttgc
     1981 taccatgaag gaaaggccaa gacaatcatc cacagctatt ccctccagca tctggttctg
     2041 tacaaaaatt aaatgcttat ttgtttaagt ctc
//
