LOCUS       NM_005928               1936 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens milk fat globule EGF and factor V/VIII domain
            containing (MFGE8), transcript variant 1, mRNA.
ACCESSION   NM_005928
VERSION     NM_005928.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Liu,W., Ren,Y., Wang,T., Wang,M., Xu,Y., Zhang,J., Bi,J., Wu,Z.,
            Lv,Y. and Wu,R.
  TITLE     MFG-E8 induces epithelial-mesenchymal transition and anoikis
            resistance to promote the metastasis of pancreatic cancer cells
  JOURNAL   Eur J Pharmacol 969, 176462 (2024)
   PUBMED   38431242
  REMARK    GeneRIF: MFG-E8 induces epithelial-mesenchymal transition and
            anoikis resistance to promote the metastasis of pancreatic cancer
            cells.
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Mizote,Y., Inoue,T., Akazawa,T., Kunimasa,K., Tamiya,M.,
            Kumamoto,Y., Tsuda,A., Yoshida,S., Tatsumi,K., Ekawa,T., Honma,K.,
            Nishino,K. and Tahara,H.
  TITLE     Potent CTLs can be induced against tumor cells in an environment of
            lower levels of systemic MFG-E8
  JOURNAL   Cancer Sci 115 (4), 1114-1128 (2024)
   PUBMED   38332689
  REMARK    GeneRIF: Potent CTLs can be induced against tumor cells in an
            environment of lower levels of systemic MFG-E8.
REFERENCE   3  (bases 1 to 1936)
  AUTHORS   Datta,R., Mukherjee,D., Podolsky,M.J., Yang,C.D., Alba,D.L.,
            Singh,S., Arnold,T.D., Koliwad,S., Lizama,C.O. and Atabai,K.
  TITLE     PTP1B mediates the inhibitory effect of MFGE8 on insulin signaling
            through the beta5 integrin
  JOURNAL   J Biol Chem 300 (2), 105631 (2024)
   PUBMED   38199575
  REMARK    GeneRIF: PTP1B mediates the inhibitory effect of MFGE8 on insulin
            signaling through the beta5 integrin.
REFERENCE   4  (bases 1 to 1936)
  AUTHORS   Duran-Jara,E., Del Campo,M., Gutierrez,V., Wichmann,I., Trigo,C.,
            Ezquer,M. and Lobos-Gonzalez,L.
  TITLE     Lactadherin immunoblockade in small extracellular vesicles inhibits
            sEV-mediated increase of pro-metastatic capacities
  JOURNAL   Biol Res 57 (1), 1 (2024)
   PUBMED   38173019
  REMARK    GeneRIF: Lactadherin immunoblockade in small extracellular vesicles
            inhibits sEV-mediated increase of pro-metastatic capacities.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1936)
  AUTHORS   Xiong,Y., Wang,B., Jiao,J., Tang,M., Li,K., Jiang,R. and Huang,W.
  TITLE     Expression and clinical association of MFG-E8 and TAM receptors in
            diabetic patients with different stages of microvascular
            complication: An experimental study
  JOURNAL   Medicine (Baltimore) 102 (44), e35788 (2023)
   PUBMED   37933040
  REMARK    GeneRIF: Expression and clinical association of MFG-E8 and TAM
            receptors in diabetic patients with different stages of
            microvascular complication: An experimental study.
REFERENCE   6  (bases 1 to 1936)
  AUTHORS   Li,B.Z., Zhang,H.Y., Pan,H.F. and Ye,D.Q.
  TITLE     Identification of MFG-E8 as a novel therapeutic target for diseases
  JOURNAL   Expert Opin Ther Targets 17 (11), 1275-1285 (2013)
   PUBMED   23972256
  REMARK    Review article
REFERENCE   7  (bases 1 to 1936)
  AUTHORS   Aoki,N., Ishii,T., Ohira,S., Yamaguchi,Y., Negi,M., Adachi,T.,
            Nakamura,R. and Matsuda,T.
  TITLE     Stage specific expression of milk fat globule membrane
            glycoproteins in mouse mammary gland: comparison of MFG-E8,
            butyrophilin, and CD36 with a major milk protein, beta-casein
  JOURNAL   Biochim Biophys Acta 1334 (2-3), 182-190 (1997)
   PUBMED   9101712
REFERENCE   8  (bases 1 to 1936)
  AUTHORS   Collins,C., Nehlin,J.O., Stubbs,J.D., Kowbel,D., Kuo,W.L. and
            Parry,G.
  TITLE     Mapping of a newly discovered human gene homologous to the
            apoptosis associated-murine mammary protein, MFG-E8, to chromosome
            15q25
  JOURNAL   Genomics 39 (1), 117-118 (1997)
   PUBMED   9027496
REFERENCE   9  (bases 1 to 1936)
  AUTHORS   Couto,J.R., Taylor,M.R., Godwin,S.G., Ceriani,R.L. and
            Peterson,J.A.
  TITLE     Cloning and sequence analysis of human breast epithelial antigen
            BA46 reveals an RGD cell adhesion sequence presented on an
            epidermal growth factor-like domain
  JOURNAL   DNA Cell Biol 15 (4), 281-286 (1996)
   PUBMED   8639264
REFERENCE   10 (bases 1 to 1936)
  AUTHORS   Larocca,D., Peterson,J.A., Urrea,R., Kuniyoshi,J., Bistrain,A.M.
            and Ceriani,R.L.
  TITLE     A Mr 46,000 human milk fat globule protein that is highly expressed
            in human breast tumors contains factor VIII-like domains
  JOURNAL   Cancer Res 51 (18), 4994-4998 (1991)
   PUBMED   1909932
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK222735.1 and BU726667.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005928.3.
            
            Summary: This gene encodes a preproprotein that is proteolytically
            processed to form multiple protein products. The major encoded
            protein product, lactadherin, is a membrane glycoprotein that
            promotes phagocytosis of apoptotic cells. This protein has also
            been implicated in wound healing, autoimmune disease, and cancer.
            Lactadherin can be further processed to form a smaller cleavage
            product, medin, which comprises the major protein component of
            aortic medial amyloid (AMA). Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Jul 2015].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY141974.1, SRR3476690.512107.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000268150.13/ ENSP00000268150.8
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1764              AK222735.1         1-1764
            1765-1936           BU726667.1         17-188              c
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q26.1"
     gene            1..1936
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /note="milk fat globule EGF and factor V/VIII domain
                     containing"
                     /db_xref="GeneID:4240"
                     /db_xref="HGNC:HGNC:7036"
                     /db_xref="MIM:602281"
     exon            1..133
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1224
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /note="isoform a preproprotein is encoded by transcript
                     variant 1; sperm associated antigen 10; O-acetyl
                     disialoganglioside synthase; medin; sperm surface protein
                     hP47; breast epithelial antigen BA46; lactadherin; milk
                     fat globule-EGF factor 8 protein"
                     /codon_start=1
                     /product="lactadherin isoform a preproprotein"
                     /protein_id="NP_005919.2"
                     /db_xref="CCDS:CCDS10347.1"
                     /db_xref="GeneID:4240"
                     /db_xref="HGNC:HGNC:7036"
                     /db_xref="MIM:602281"
                     /translation="MPRPRLLAALCGALLCAPSLLVALDICSKNPCHNGGLCEEISQE
                     VRGDVFPSYTCTCLKGYAGNHCETKCVEPLGLENGNIANSQIAASSVRVTFLGLQHWV
                     PELARLNRAGMVNAWTPSSNDDNPWIQVNLLRRMWVTGVVTQGASRLASHEYLKAFKV
                     AYSLNGHEFDFIHDVNKKHKEFVGNWNKNAVHVNLFETPVEAQYVRLYPTSCHTACTL
                     RFELLGCELNGCANPLGLKNNSIPDKQITASSSYKTWGLHLFSWNPSYARLDKQGNFN
                     AWVAGSYGNDQWLQVDLGSSKEVTGIITQGARNFGSVQFVASYKVAYSNDSANWTEYQ
                     DPRTGSSKIFPGNWDNHSHKKNLFETPILARYVRILPVAWHNRIALRLELLGC"
     exon            134..265
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /inference="alignment:Splign:2.1.0"
     exon            266..447
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /inference="alignment:Splign:2.1.0"
     exon            448..600
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /inference="alignment:Splign:2.1.0"
     exon            601..745
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /inference="alignment:Splign:2.1.0"
     exon            746..930
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1086
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1936
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1912..1917
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /note="hexamer: AATATA"
     polyA_site      1936
                     /gene="MFGE8"
                     /gene_synonym="BA46; EDIL1; HMFG; hP47; HsT19888; MFG-E8;
                     MFGM; OAcGD3S; SED1; SPAG10"
                     /note="major polyA site"
ORIGIN      
        1 agaaccccgc ggggtctgag cagcccagcg tgcccattcc agcgcccgcg tccccgcagc
       61 atgccgcgcc cccgcctgct ggccgcgctg tgcggcgcgc tgctctgcgc ccccagcctc
      121 ctcgtcgccc tggatatctg ttccaaaaac ccctgccaca acggtggttt atgcgaggag
      181 atttcccaag aagtgcgagg agatgtcttc ccctcgtaca cctgcacgtg ccttaagggc
      241 tacgcgggca accactgtga gacgaaatgt gtcgagccac tgggcctgga gaatgggaac
      301 attgccaact cacagatcgc cgcctcgtct gtgcgtgtga ccttcttggg tttgcagcat
      361 tgggtcccgg agctggcccg cctgaaccgc gcaggcatgg tcaatgcctg gacacccagc
      421 agcaatgacg ataacccctg gatccaggtg aacctgctgc ggaggatgtg ggtaacaggt
      481 gtggtgacgc agggtgccag ccgcttggcc agtcatgagt acctgaaggc cttcaaggtg
      541 gcctacagcc ttaatggaca cgaattcgat ttcatccatg atgttaataa aaaacacaag
      601 gagtttgtgg gtaactggaa caaaaacgcg gtgcatgtca acctgtttga gacccctgtg
      661 gaggctcagt acgtgagatt gtaccccacg agctgccaca cggcctgcac tctgcgcttt
      721 gagctactgg gctgtgagct gaacggatgc gccaatcccc tgggcctgaa gaataacagc
      781 atccctgaca agcagatcac ggcctccagc agctacaaga cctggggctt gcatctcttc
      841 agctggaacc cctcctatgc acggctggac aagcagggca acttcaacgc ctgggttgcg
      901 gggagctacg gtaacgatca gtggctgcag gtggacctgg gctcctcgaa ggaggtgaca
      961 ggcatcatca cccagggggc ccgtaacttt ggctctgtcc agtttgtggc atcctacaag
     1021 gttgcctaca gtaatgacag tgcgaactgg actgagtacc aggaccccag gactggcagc
     1081 agtaagatct tccctggcaa ctgggacaac cactcccaca agaagaactt gtttgagacg
     1141 cccatcctgg ctcgctatgt gcgcatcctg cctgtagcct ggcacaaccg catcgccctg
     1201 cgcctggagc tgctgggctg ttagtggcca cctgccaccc ccaggtcttc ctgctttcca
     1261 tgggcccgct gcctcttggc ttctcagccc ctttaaatca ccatagggct ggggactggg
     1321 gaaggggagg gtgttcagag gcagcaccac cacacagtca cccctccctc cctctttccc
     1381 accctccacc tctcacgggc cctgccccag cccctaagcc ccgtccccta acccccagtc
     1441 ctcactgtcc tgttttctta ggcactgagg gatctgagta ggtctgggat ggacaggaaa
     1501 gggcaaagta gggcgtgtgg tttccctgcc cctgtccgga ccgccgatcc caggtgcgtg
     1561 tgtctctgtc tctcctagcc cctctctcac acatcacatt cccatggtgg cctcaagaaa
     1621 ggcccggaag cgccaggctg gagataacag cctcttgccc gtcggccctg cgtcggccct
     1681 ggggtaccat gtggccacaa ctgctgtggc cccctgtccc caagacactt ccccttgtct
     1741 ccctggttgc ctctcttgcc ccttgtcctg aagcccagcg acacagaagg gggtggggcg
     1801 ggtctatggg gagaaaggga gcgaggtcag aggagggcat gggttggcag ggtgggcgtt
     1861 tggggccctc tatgctggct tttcacccca gaggacacag gcagcttcca aaatatattt
     1921 atcttcttca cgggaa
//
