LOCUS       NM_001321154            9770 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens methyltransferase 8, tRNA N3-cytidine (METTL8),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001321154 XM_006712763
VERSION     NM_001321154.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Lee,B.W.L., Chuah,Y.H., Yoon,J., Grinchuk,O.V., Liang,Y.,
            Hirpara,J.L., Shen,Y., Wang,L.C., Lim,Y.T., Zhao,T., Sobota,R.M.,
            Yeo,T.T., Wong,A.L.A., Teo,K., Nga,V.D.W., Tan,B.W.Q., Suda,T.,
            Toh,T.B., Pervaiz,S., Lin,Z. and Ong,D.S.T.
  TITLE     METTL8 links mt-tRNA m3C modification to the HIF1alpha/RTK/Akt axis
            to sustain GBM stemness and tumorigenicity
  JOURNAL   Cell Death Dis 15 (5), 338 (2024)
   PUBMED   38744809
  REMARK    GeneRIF: METTL8 links mt-tRNA m[3]C modification to the
            HIF1alpha/RTK/Akt axis to sustain GBM stemness and tumorigenicity.
            Erratum:[Cell Death Dis. 2024 Jul 31;15(7):547. doi:
            10.1038/s41419-024-06917-x.. PMID: 39085240]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   Huang,M.H., Peng,G.X., Mao,X.L., Wang,J.T., Zhou,J.B., Zhang,J.H.,
            Chen,M., Wang,E.D. and Zhou,X.L.
  TITLE     Molecular basis for human mitochondrial tRNA m3C modification by
            alternatively spliced METTL8
  JOURNAL   Nucleic Acids Res 50 (7), 4012-4028 (2022)
   PUBMED   35357504
  REMARK    GeneRIF: Molecular basis for human mitochondrial tRNA m3C
            modification by alternatively spliced METTL8.
REFERENCE   3  (bases 1 to 9770)
  AUTHORS   Lentini,J.M., Bargabos,R., Chen,C. and Fu,D.
  TITLE     Methyltransferase METTL8 is required for 3-methylcytosine
            modification in human mitochondrial tRNAs
  JOURNAL   J Biol Chem 298 (4), 101788 (2022)
   PUBMED   35247384
  REMARK    GeneRIF: Methyltransferase METTL8 is required for 3-methylcytosine
            modification in human mitochondrial tRNAs.
REFERENCE   4  (bases 1 to 9770)
  AUTHORS   Kleiber,N., Lemus-Diaz,N., Stiller,C., Heinrichs,M., Mai,M.M.,
            Hackert,P., Richter-Dennerlein,R., Hobartner,C., Bohnsack,K.E. and
            Bohnsack,M.T.
  TITLE     The RNA methyltransferase METTL8 installs m3C32 in mitochondrial
            tRNAsThr/Ser(UCN) to optimise tRNA structure and mitochondrial
            translation
  JOURNAL   Nat Commun 13 (1), 209 (2022)
   PUBMED   35017528
  REMARK    GeneRIF: The RNA methyltransferase METTL8 installs m(3)C32 in
            mitochondrial tRNAs(Thr/Ser(UCN)) to optimise tRNA structure and
            mitochondrial translation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9770)
  AUTHORS   Scholler,E., Marks,J., Marchand,V., Bruckmann,A., Powell,C.A.,
            Reichold,M., Mutti,C.D., Dettmer,K., Feederle,R., Huttelmaier,S.,
            Helm,M., Oefner,P., Minczuk,M., Motorin,Y., Hafner,M. and
            Meister,G.
  TITLE     Balancing of mitochondrial translation through METTL8-mediated m3C
            modification of mitochondrial tRNAs
  JOURNAL   Mol Cell 81 (23), 4810-4825 (2021)
   PUBMED   34774131
REFERENCE   6  (bases 1 to 9770)
  AUTHORS   Xu,L., Liu,X., Sheng,N., Oo,K.S., Liang,J., Chionh,Y.H., Xu,J.,
            Ye,F., Gao,Y.G., Dedon,P.C. and Fu,X.Y.
  TITLE     Three distinct 3-methylcytidine (m3C) methyltransferases modify
            tRNA and mRNA in mice and humans
  JOURNAL   J Biol Chem 292 (35), 14695-14703 (2017)
   PUBMED   28655767
  REMARK    GeneRIF: Data, including data from studies using mutant/knockout
            mice and cell lines from such mice, suggest that METTL2 and METTL6
            (but not METTL8) contribute to post-transcriptional methylation of
            cytidine (formation of 3-methylcytidine) in tRNA; METTL2 methylates
            tRNA(Thr) and tRNA(Arg); METTL6 interacts with seryl-tRNA
            synthetase and methylates tRNA(Ser); METTL8 catalyzes
            post-transcriptional methylation of cytidine in mRNA.
REFERENCE   7  (bases 1 to 9770)
  AUTHORS   Jakkaraju,S., Zhe,X., Pan,D., Choudhury,R. and Schuger,L.
  TITLE     TIPs are tension-responsive proteins involved in myogenic versus
            adipogenic differentiation
  JOURNAL   Dev Cell 9 (1), 39-49 (2005)
   PUBMED   15992539
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK024046.1, BC025250.1, AK124092.1, AC007739.2 and BM930105.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001321154.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7410570.210093.1,
                                           SRR1803611.131632.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375258.9/ ENSP00000364407.4
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-108               AK024046.1         121-228
            109-317             BC025250.1         252-460
            318-1728            AK124092.1         512-1922
            1729-9424           AC007739.2         25174-32869
            9425-9770           BM930105.1         263-608
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.1"
     gene            1..9770
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /note="methyltransferase 8, tRNA N3-cytidine"
                     /db_xref="GeneID:79828"
                     /db_xref="HGNC:HGNC:25856"
                     /db_xref="MIM:609525"
     exon            1..108
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     exon            109..263
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..1344
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /note="isoform 1 is encoded by transcript variant 2;
                     methyltransferase-like protein 8;
                     tension-induced/inhibited protein; mRNA
                     N(3)-methylcytidine methyltransferase METTL8;
                     methyltransferase like 8; tRNA N(3)-methylcytidine
                     methyltransferase METTL8, mitochondrial; methyltransferase
                     8, methylcytidine; tRNA N(3)-cytidine methyltransferase
                     METTL8, mitochondrial"
                     /codon_start=1
                     /product="tRNA N(3)-cytidine methyltransferase METTL8,
                     mitochondrial isoform 1"
                     /protein_id="NP_001308083.1"
                     /db_xref="CCDS:CCDS2242.2"
                     /db_xref="GeneID:79828"
                     /db_xref="HGNC:HGNC:25856"
                     /db_xref="MIM:609525"
                     /translation="MNMIWRNSISCLRLGKVPHRYQSGYHPVAPLGSRILTDPAKVFE
                     HNMWDHMQWSKEEEAAARKKVKENSAVRVLLEEQVKYEREASKYWDTFYKIHKNKFFK
                     DRNWLLREFPEILPVDQKPEEKARESSWDHVKTSATNRFSRMHCPTVPDEKNHYEKSS
                     GSSEGQSKTESDFSNLDSEKHKKGPMETGLFPGSNATFRILEVGCGAGNSVFPILNTL
                     ENSPESFLYCCDFASGAVELVKSHSSYRATQCFAFVHDVCDDGLPYPFPDGILDVILL
                     VFVLSSIHPDRMQGVVNRLSKLLKPGGMLLFRDYGRYDKTQLRFKKGHCLSENFYVRG
                     DGTRAYFFTKGEVHSMFCKASLDEKQNLVDRRLQVNRKKQVKMHRVWIQGKFQKPLHQ
                     TQNSSNMVSTLLSQD"
     exon            264..355
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     exon            356..726
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     exon            727..776
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     exon            777..840
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     exon            841..980
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1087
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1153
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..9770
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1708..1713
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /note="hexamer: AGTAAA"
     polyA_site      1729
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /note="major polyA site"
     regulatory      3880..3885
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /note="hexamer: AATAAA"
     polyA_site      3901
                     /gene="METTL8"
                     /gene_synonym="TIP"
     polyA_site      5867
                     /gene="METTL8"
                     /gene_synonym="TIP"
     regulatory      6090..6095
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /note="hexamer: AATAGA"
     polyA_site      6115
                     /gene="METTL8"
                     /gene_synonym="TIP"
     regulatory      7146..7151
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /note="hexamer: AATAAA"
     polyA_site      7179
                     /gene="METTL8"
                     /gene_synonym="TIP"
     regulatory      8085..8090
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /note="hexamer: AATAAA"
     polyA_site      8114
                     /gene="METTL8"
                     /gene_synonym="TIP"
     regulatory      9746..9751
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL8"
                     /gene_synonym="TIP"
                     /note="hexamer: ATTAAA"
     polyA_site      9770
                     /gene="METTL8"
                     /gene_synonym="TIP"
ORIGIN      
        1 actcacaagc ttctcggccc cgaccttcgc cctgggaggt tctggccagg tgccgggagg
       61 ggcgctgtgt cgagggcgat ccccccaaag cagcgtcccg tgctaaaggt actgcttagg
      121 atgaatatga tttggagaaa ttccatttct tgtctaaggc taggaaaggt gccacacaga
      181 taccaaagtg gttaccaccc agtggcccct ctgggatcaa ggattttaac tgacccagcc
      241 aaagtttttg aacacaacat gtgggatcac atgcagtggt ctaaggaaga agaagcagca
      301 gccagaaaaa aagtaaaaga aaactcagct gtgcgagtcc ttctggaaga gcaagttaag
      361 tatgagagag aagctagtaa atactgggac acattttaca agattcataa gaataagttt
      421 ttcaaggatc gtaattggct gttgagggaa tttcctgaaa ttcttccagt tgatcaaaaa
      481 cctgaagaga aggcgagaga atcatcatgg gatcatgtaa aaactagtgc tacaaatcgt
      541 ttctcaagaa tgcactgtcc tactgtgcct gatgaaaaaa atcattatga gaaaagttct
      601 ggttcttcag aaggtcaaag caaaacagaa tctgattttt ccaacctaga ctctgaaaaa
      661 cacaaaaaag gacctatgga gactggattg tttcctggta gcaatgccac tttcaggata
      721 ctagaggttg gttgtggagc tggaaatagt gtgtttccaa ttttgaacac tttggagaac
      781 tctccggagt cctttctgta ttgttgtgat tttgcttctg gagctgtgga gctcgtaaag
      841 tcacactcgt cctacagagc aacccagtgt tttgcctttg ttcatgatgt atgtgatgat
      901 ggcttacctt acccttttcc agatgggatc ctggatgtca ttctccttgt ctttgtgctc
      961 tcttctattc atcctgacag gatgcaaggt gttgtaaacc gactgtccaa gttactgaaa
     1021 cctgggggaa tgctgttatt tcgagactat ggaagatatg ataagactca gcttcgtttt
     1081 aaaaagggac attgtttatc tgaaaatttt tatgttcgag gagatggtac cagagcatat
     1141 ttctttacaa aaggggaagt ccacagtatg ttctgcaaag ccagtttaga tgaaaagcaa
     1201 aatctggttg atcgccgctt acaagttaat aggaaaaaac aagtgaaaat gcaccgagtg
     1261 tggattcaag gcaaattcca gaaaccattg caccagactc agaatagctc caatatggta
     1321 tctacactcc tttcacaaga ctgaactttg taacatgtta aggtacaaag ccagaggact
     1381 gtgctattca aggactactg taagtctatt gtttctcaaa agacaatgag aaaaaaagaa
     1441 gagaatttgt atttcctgcc gttttgtcat aggtgagctc ctttgtgcat tttaagcaca
     1501 tgtaagtggt tcagcacagt atgccttttt ctgtgctttg aaaacttgat atgctcaagc
     1561 ttgtttgaat ttattacatc taaccatttt gcttgttcct tgatttttat aagcattcaa
     1621 ttaagttagt attatgtcaa gtaattttga gaaaatgtaa cttgacattt tttgcaagta
     1681 aaaaaaattg tttatttgtt taggcttagt aaaccagttc ccaaacacag tcagactctt
     1741 cccattgtca tctgattgca gagagaaagc acaccttatt tccagggaaa gctacaacaa
     1801 gcccaaggtc aaagtgtatt attttttgtc ttgttgttgt ctattttctc ccaatttttt
     1861 tttgaaattc agaggctcat atctgaaata gaattttagt tcctctttcc tttcctaaaa
     1921 ttggggaagt acagcccatg ctgacattat tttcaggcta ttcttagata tacaagttgt
     1981 taggccaggt gcaatggctc gcacctgtaa tcccagcact ttggggggct gaggcaggca
     2041 gatcggttga gctcaggagt tcaagaccag cctggacaac atggcaaaac cctgtctctc
     2101 ccaagaatac aaaaattagc caggcatggt ggcacacacc tgtggtccca gctactcagg
     2161 agactgaggt gggaggatcg cttgagcctc ggaggcggag gttgcagtga gctgagattg
     2221 taccactgcg ctccaaactg ggtgacatgg tgagaccttg tctccaaaaa aaaaaaaaaa
     2281 aaaaaaaaag gtatgtaagc tgacaagctc ttgtatcaag ggcttttgaa ccagagcaac
     2341 ttcatcttga atagggactg tgtaaaatga agctgagacc tactaggctg caatcccagg
     2401 aggttaagac attcttagtc acaggatgag ataggaagtc agtacaagat acaggtcata
     2461 cagaccttgc tgataaaaca ggttgcagtg aagaagccgg ccaaatgcca ccaaaaccaa
     2521 gatggcaatg agagtgtcct ctggctgtcc tcactgctac actcccacca gtgccatgat
     2581 agtttacaaa tgccatggca acatcaggaa gttacccata tggtctacaa aggggaggcc
     2641 tgaataatcc accccttgtg tagcatataa tcaagaaata accttaaaaa tgggcaacca
     2701 gtagcccttg gggctgctct gcctatggag tagccattct ttatgtttta cttttttttt
     2761 tttttttttt ttgagatgga gtcttgctct ggcacccagg ctggagtgca gtggcgtgat
     2821 ctcagctcac tgccagctcc acctcccggg ttcacgccat tctcttgctt cagcctcctg
     2881 agtagctggg actacaagca cccaccacca tgcccagcta attttttttt tttttgtatt
     2941 tttagtagag acggggtttc accatgttag ccaggatggt ctcaatctcc tgaccttgtg
     3001 atccgcccgc ctcggcctcc caaagtgctg ggattacagg tgtgagccac cgcgcccagc
     3061 ctattcttta cttaaacttg ttttcacttt gctgtatgga ctcaattctt tcttgcacaa
     3121 gatccaagaa tcctctcttg gcgtctggat ccagaccctt tctggtatca gtggtaagct
     3181 ctgtttaaga caactgtcat acccaagaga attcttaaat tgtggcagtt aaaaatgtat
     3241 ttatcccctc gagggccggg cgcagtcgct caagcccgta atcccagcac tttgggaggc
     3301 cgaggaggcg gatcatgagg tcaggagttc aagaccagcc tgaccaacac tgtgaaaccc
     3361 tgtctctact aaaaatacaa aaaaaattag ccaggcgtgg tggtgggcac ctgtaatccc
     3421 agctactcgg gaggctgagg caggagaatc acttgaaccc gggaggcaga ggttgcagtg
     3481 agccgagatg acaccactgc cctccagcct gggtgacagt tgttagactg cgtatcaaaa
     3541 aaaaaaaaaa aaatttattt actcccttgc ttcaaaaagg acataagttg gcttgcaaat
     3601 cacattttgt agaaatgaag tttttaagat tagtctcata ctcattacat aatatttatt
     3661 gactacttac atattgagtg ctaaattcag tggaataata tgaacaataa agacccagcc
     3721 ttgtttatgc tcttcaggga gcataaatag agtacagctg gcaaaacaaa atgtgcatat
     3781 cccaaacctg taaatcatga tctaagacaa tatacaaaca ctcaaatagg gaacttacca
     3841 gctagcagac tttgattatg aaccacagaa cctagaataa ataaatactc tcttgaaaga
     3901 accatcattg gaataattaa gttccaactg ttttttcttt ctgaattcac ttaaaatcca
     3961 atttctagaa gaatatcatt ggtccagctt tagtcattgg ttcatctctt aacaggctgg
     4021 ggcagggcag cttaattgat gatgccacca gaatgggcaa catgggaaaa accctatctc
     4081 taccaaaaat acaaaaatta gccagtgatg gtggcacgca cctgttgtcc cagctactca
     4141 ggaggctgag ctgggaggat cttttgagcc tgggaggcag aggttgcagt gagctgagat
     4201 cgcaccactg cactccaacc tggagtggaa ggaaatttca cttcctggaa tggaagaaag
     4261 ttcttcaaag gaaaatcaag acactgctgt caaaaaacac caggggaggg atgctgtcca
     4321 gtcacagcca acacatcata tagcagtggt agcagtagta atagtatttc cagagagaga
     4381 gagagagatt agctctgaaa aagaatggag aagaatcagg tttcttgggg gaggtaggtc
     4441 tcaggctagt tcttgaatgt acattctcag cagatggtga gaagacaagt gtggtgtggg
     4501 acattaatga tgagggatag gtagaaaaga tgggaagggg gtcagcttgt agataaccta
     4561 gactagcagg tgtatgactt ctagtctctg aactggagcc atctgagatc agtttgagat
     4621 atggtgtctt ataaatgtat tgagagaggt tgaggagttg tctatagaat ggaatggagt
     4681 gaggtcagag atcagtggca aggaagacca cataaaggtt tttgcacaaa cttgccttga
     4741 tggcaagtcc tcaaaagtac aattatcaag ctttctgttt aaacatgcat aagtcagtaa
     4801 atataaacat taaaatttgc ccaagactct taactcagtt gtatttcata attaaagtgt
     4861 acattaaaaa gccaaactag tcatgaaagt attacataca gcaagggcag tttagataag
     4921 ttaatgttgc tatgggaacc ccagcagatc tccttgtgtc tccgtttaaa aatcatggcc
     4981 caaggtggtt tcaaaaacaa cagaaggtaa aattattctc acattctcac acatgtaaat
     5041 tgtactgtct ctctcagttc taaatagcgg attcaagcca gcctcttgac tggcacagta
     5101 aaattcggct tcgtgtcttt ctataatgta ttacagcatg tgagttaacc tttagaagct
     5161 tcaaaaatac atcaaccaaa tttggactgt gagacacaaa ctggtctctt ttgggctctg
     5221 tccagcatag ccttcctatt ccacatggct gcgcagcagg gccatttcca ccagtgtctt
     5281 ctgcttgcct ggtcagtctt tctgtctctt aacctaccta caacaacttc cttctcgggg
     5341 tttgctgcta gatagaaatg tagattttgg cacagaaaga aaagggaaag acctatttgg
     5401 aattttgtct atagatgtta agaaatcatg aagcgttgca aatgtaactg cttgggtatt
     5461 agtttaaaat atttgataac tgcatgaaat tctaatttac ccctcacatc cataaagcta
     5521 ggaccagttg gttaggcaga gtttgatatt taaaaattca tcttctgaga gagctgaatt
     5581 tcaaaaggac cattttcttt ccaggagtaa aaggtatatt ctttcatctt cttacagtac
     5641 agaaacaatg gaatatattg tttaaatgca cccttcattt ttttctccat ttctaaaaat
     5701 tcacttttga gcatcgtcca aatgtaaaaa ttctcacaca tgaggaactg tttccgaagg
     5761 ttgtgagaaa gtcaagataa cagtttctat tattaaaagt tttgcattcg gtatgacaca
     5821 tttgcctgta ttgaaacacc catttaacat tgacttctga gaacttattt gctttgcctc
     5881 tctcgattcc atatgctttg tctacaaatt gtgaacacgt cccggtttct acccgtgtgt
     5941 gattctaagt cgttgctgta ctctagcttg tacgtgaaaa ttcacccata tgcagatagc
     6001 tgccctcttg gaggcaaaac ttaaagctat cagaaccatc tggattgtta aaccccaaaa
     6061 cttccaactc atcaaagtcc tgaagcctga atagatcaca cattgttact tctaattggt
     6121 tttgatgttt gttatgctcc tcacaagtgc agataacttc acatttgcat cactaatcga
     6181 cctctttcaa agcctctgct cctcctgctt cctgcctctg ttagtcatca ggagacttag
     6241 tgttggcgtg ggtattttcc ccgcttgtga aagtgcattg tcaattctag agcattaaat
     6301 atatgtgagg tattgttatt aatctacaag gcagtacaca cagtattcca gcaacagtga
     6361 ctttttcaaa ggttttatga gacattgtaa gaaagacagt atctttccat ggaaaaaagt
     6421 gttgcctcag agtcaaacag gaagcatggg tcctagttcc tattgaactg cttagctact
     6481 cttaatttag ctctgaatgc tagattttgt gtatactata cacattattt aatcctcaaa
     6541 ctcctaggaa ttcattttga tgataaccat ttacagaaga gatgttgggt aacttgctca
     6601 ggataccaca tctagtaagt agcagagtca caactcggac ccagaactct ctgattccaa
     6661 aacctctact tgctgctctt gtaatggatg ggcccaaatg ctgctgggaa aggcaccagg
     6721 gaagaaagag ctttctgagt caagcagaaa acgatagcag tggagaggag tttgtttcag
     6781 acgaccttca aggttatggc tcattctaga tccaatcatt ctagcaaata agtaggttac
     6841 acttgtccac aattacaaat tggaaaagaa gagaaggaaa ggaactgaga ggaatgacaa
     6901 agcaaaaagc tttcaatcag ttcttttcag cattctgctt ctgcctagtt ctgcactcgc
     6961 cccaccccct caggcttcag ccttccactt ggcatccctc ctcgcagagc actctcactt
     7021 gcatactcct cgcttaaaga aaaaaagcat tctgtactgc agtggggaaa aaaaaccctt
     7081 aaaatgtctt caaaaggatt cagctctagg gtaatgtaaa atatgtgaaa tcaggaggtg
     7141 tccataataa aatatgagtg aaaacatcgt tatttaaaac attgtatgta cattaatgcc
     7201 acattaatac caattcaggc atgaaatgtt gagcatttct tggaatacgg tttcttggaa
     7261 tatggttggg gatttgagag gcgcaaaggt acccgcaaga tggctttggt gtagttataa
     7321 atataaatag aagtaaaata acagttgcat cttcatttat aatgaatcta tattgtggcc
     7381 tcaaagctac agagaaaagt ggatgccaaa atctgtcttt cattaacata tgtcttaaat
     7441 gacaaggaag aggtgttgaa gataaaatta ctttttccta tgagtatgaa tcagcaacat
     7501 gcagtaacaa gcaagtaata atagttctgc gtgtgagtgc ctacagaggg taagaaaaat
     7561 agcatcagat tgaaaaagca agtgcacctg cagaagggac ccaggaggct gcagagcaca
     7621 gctcccttga gcatcatctc aaagtgtctc agagcacagt gaccctaatt ctggcaagtc
     7681 aggttggcat gagatggatg gagcacactg aaatctccag atggtcagac agcccaccct
     7741 cagctgcccc tggagctcgg caaaggcaag ctacaaggca ctgctacttg gccaagagtt
     7801 taaaacaaca gcttctgatt ctacagtgtc tgtggtttca caaccactta accttaagta
     7861 aggaacagct agagttagct gaatgaataa atacacgtta acgttacata aagagggaaa
     7921 aaaagcaata cttgagcaca tagacttcaa atagtgtcca gctagctgtg tccttggcat
     7981 cccagtgatc tgggtcttta tctttgattt tctccagcag gtaggcactg agtagatcca
     8041 gggcccaggg cgggaaatgc ttctgtatca gatgttacct atcaaataaa atgagccttt
     8101 gattgcctcc taaagctcca gcatcgtctt tcctagttgg gctgatgagg tttggtccag
     8161 aaaaatggaa ttttgttatg cttataaaaa ttatttggcc aaatattttc aatccattgc
     8221 tcctagttca ttgcagaaaa cgcagaaatt aattttttcc tcagttaacc tttatatatc
     8281 aagtgttggt tgtttcaaaa aacactggag gacagtcacc ctttgggctt ttaaggcttt
     8341 tccgctggaa gcaagcacta aacctgtgtg acttcctaaa attagcaatg agcccgctgc
     8401 tgagtccact gcattagtgg ggcttttttg ttttttgttt ttaatgctct gtacttagca
     8461 caagcaacac tatgtaattg ctgcctcatt tctctcacct tctgcctcct gagagcaact
     8521 tgtgttcctc ctgaggcagt cctgctaaac agaatgcttg gcgtaaagaa tgcgatcgct
     8581 ctccttacat ctttagttgt gccctgctcc ctcaggtgag cagagacata tccccctaag
     8641 cagcatttgg cactcgcact gaaaattgcc tgaaagagaa tggctgaaat tgccagtatc
     8701 acatgctgca ttgaaacaag aaagaaattt ctgcctccaa ggataattgc ttttctcatt
     8761 gctcatactt tttctttcgc agccctcatt tattttaata tttatatctt tactttgact
     8821 ttctacaaga tattttttaa gtttcttttt aatccaaatg aaagatgaaa ttcaggatcc
     8881 caaaaagcaa ggaagatcta tcctttccat gaatatattc ttaatccacc actctctgag
     8941 atgtctcttc atttgcagct ttgtttctta atagaacacc tgggctctgg agattagtga
     9001 gacaaagctt cattataatc ttccttgttt tactcacatt tcttccatgc tttctctccc
     9061 taactcactc tccaggtgct tctttctctt gccaaatagt gcaaggaaat acttttgcag
     9121 atcttttgtt tactttagga aaaaaccatg gatccaggct tccccattca gcactgctgc
     9181 tcatttcctg actggccttt acagtttggc tcagtagcaa cttggggact tgttttctac
     9241 ccacaggaaa ggagctgggt gcccggcagc cccatgccgg catgagaatt aagtcctgct
     9301 cattccctgg ataagcaagc tgacgttttc ctcatagatt ttcttttcca tttctcaaag
     9361 ctttattgca tactcattta gctttgactt ttaaaaagac attttaatag tgttgttttt
     9421 cctccaagca tgtgcaaaag ctgatcatgt gcacgaggaa gctctcgaga ataaagaatt
     9481 acctctttgc tgtttttcac ctactacgtc acacactaca caacacaccc agggcttacc
     9541 cctactccag tgtcattggc aagtgcctgg cctggtatag gaggaactgt gacaccagag
     9601 gaatttggct tgggttacct cactttctag gttcccaaga ttcccaagtt aaggaagctt
     9661 tggttattac atagttgaac attttcttat ttcatgtaag ttttgtaaat cttctgtgat
     9721 ttcttctcct ctttggcaca tttacattaa attttattct agttatgtaa
//
